To introduce the international registration condition of acupuncture clinical research. With the examples of World Health Organization International Clinical Trials Registry Platform and the U. S. National Institutes of Health Clinical Registration Platform, the registration method and current condition of acupuncture clinical trials in international clinical trials registration platform were analyzed. The results indicate that the number of acupuncture clinical trials registration is gradually increased and the registration number from China is on the rise as well. But most domestic acupuncture clinical researches haven't been registered arid the researchers' valuing degree for clinical trials registration and methodology research needs to be improved.
Acupuncture Therapy ; standards ; Biomedical Research ; legislation & jurisprudence ; standards ; Clinical Trials as Topic ; legislation & jurisprudence ; standards ; Humans ; Registries

Atherosclerosis ; complications ; pathology ; Humans ; Thrombosis ; complications ; pathology

BACKGROUND: Biliary complications are the common complication of liver transplantation. However, it is difficult to find the complications, since no obvious characteristic signs on the patients. Magnetic resonance imaging (MRI), especially,magnetic resonance cholangiopancreatography (MRCP) may provide a comprehensive evaluation of the functions and complications of the transplanted liver as a safe, noninvasive and exact technique.OBJECTIVE: To evaluate the manifestation of biliary system after liver transplantation by MRCP and its value of diagnosis of biliary complications.DESIGN: Comparative observation.SETTING: Medical Imaging Center, Nanfang Hospital, Southern Medical University.PARTICIPANTS: A total of 13 male liver transplantation patients aged from 40-58 years, who received imaging examination at Medical Imaging Center, Nanfang Hospital, Southern Medical University from January 2003 to January 2005, were enrolled. Original causes of liver failure included cirrhosis (n =11) and primal hepatic carcinoma (n =2). Ten received orthotopic liver transplantation, while 3 received piggyback orthotopic liver transplantation. Anastomotic mode of bile duct was common bile duct end-to-end anastomosis. The interval between transplantation and MRI examination ranged from 15 days to 1 year. All patients knew the items of examination and agreed to participate in the experiment.METHODS: Thirteen patients with orthotopic liver transplantation underwent turbo spin-echo (TSE) and fast low angle shot (FLASH), MRCP, Gd-DTPA enhanced MRI to analyze the characteristics of each imaging after liver transplantation.MAIN OUTCOME MEASURES: The imaging findings of the reestablished bile duct and complications of bile duct at MRCP.RESULTS: The diameters of bile duct and caliber were normal, no stricture on anastomotic stoma in 3 patients.Thick-section planes of MRCP did not develop biliary duct of one case. Biliary dilation occurred in 8 patients. There were 5 cases of biliary strictures and 2 cases of choledochus stone. Mucocele of bile duct remnant was identified in 2 cases.Bile leakage occurred in 1 case after T-tube was removed. Rejection reaction occurred in 2 cases.CONCLUSION: MRCP not only can display the image of reestablished biliary tract after liver transplantation, but also can exactly diagnose complications rapidly.

This paper deals with the analysis of the phospholipids and fatty acids in Hippocomnpus kelloggi Jordan et Synder, H. Histrix Kaup, II. trimaculalus Leach, II. kuda Bleeder, II. japonicus Kaup, Solenognathus hardwickii(Gray), Syngnathoides biacufcatus(Bloch) and Tracliyrliamphus serralus (Temminck &. Schlegel).The total phospholipids were extracted with Folch solvent and determined by molybdate blue spec-trophotometric method. The result showed that the contents of total phospholipids in these spccimins were 2. 56-7. 82mg/g.The nine kinds of phospholipid standards of absorbance proportional coefficient were detected in this paper. The phospholipid components were separated and determined by using thin - layer chro-matographic method and Vaskovsky solvent as a specific spray reagent. The quantitative analysis of the distribution of various phospholipids components in these specimen ts were carried out, by using TLC scanning and the corrective method of absorbance proportional coefficient. The resut showed that the Hippocampus contains about five phospholipids: lysophosphatidylcholine, sphingornyelin, phophatidylcholine, phosphatidylinositol, and phosphatidylethanolamine. The phosphatidylserine , phosphatidylglycerol, diphosphatidylglycerol and phosphatidic acid were more in Syugnathus than in Hippocampus, But in all these specimens, the main phospholipid components were lysophos- phatidylcholine, sphingornyelin and phosphatidylcholine.Fourteen fatty acid compositions in these specimens were analysed by GC -MS -DS. The result showed that the main fatty acid were hexadecanoic acid, 9 - octadecenoic acid, 8,11- octadeca-dienoic acid and 1,7,10,13,16,19 - docosahexaenoic acid.

A 15-year-old male patient presented with intermittent red secretion on the face, upper limbs, nail grooves, fingers and toes for more than 10 months. Ten months prior to the presentation, red secre-tions began to emerge on the face, especially ears, eyes and the surrounding area of nose, following a severe psychical stimulus. The attack typically lasted 2-3 minutes before spontaneous stop with the formation of blood crusts. The frequency was 1-4 per day during the attack of hemathidrosis which might last 1 week. The interval was irregular and varied from 10 days to months. There was no blood stasis, bruise or wound left on the skin after the wipe of bloodstain, and no itch or pain was complained of. Before the secretion of bloody sweat, the patient was always fidgety, irritable, and sometimes excited or difficult to sleep, but the secretion never occurred at night. After several episodes, bloody sweat also appeared in succession and symmetrically on the forearms, extensor crura, fingers, toes and perionychium. The cell components of bloody sweat were identical to those of peripheral blood. The patient was diagnosed as hemathidrosis. No abnormality was found in labora-tory tests, histopathological examination, electron microscopy or radiology. No relief of symptoms was achieved after treatment with vitamin C, rutoside and oryzanol. Hemathidrosis is a rare disease, and to clarify its patho-genesis is essential for its treatment.

Objective To study the influence of dietary test designed by Nutritional Department of Henan Provincial People's Hospital on diabetic inpatients' dietary cognition. Methods A total of 134 diabetic inpatients were selected and tested with standard diabetic meaL Self-reporting of oil, salt, staple food, vegetables and meat intake was evaluated. Nonparametric tests were used for statistical analysis. Results There were significant differences in self-reporting of oil,salt,staple food and meat intake between pre and post test (Z= -4.642, -8.700, -2.218, -5. 528,all P<0.05) ; however,no significant change of self reporting vegetable intake was found ( Z = - 1. 385, P > 0. 05 ). Conclusion Dietary test can significantly change patients' cognition about their daily dietary intake.

Objective:To screen and identify the potential targets of carthamin against sepsis by studying the characteristics of carthamin.Methods:The pharmacological parameters and molecular characteristics of carthamin were analyzed with the aid of Traditional Chinese Medicine Systems Pharmacology (TCMSP). The targets of carthamin were screened by SwissTargetprediction (a website providing compound target prediction) and Drug Repositioning and Adverse drug Reaction via Chemical-Protein Interactome (DRAR-CPI). The anti-sepsis targets were selected from the three databases of Online Mendelian Inheritance in Man (OMIM), Comparative Toxicogenomics Database (CTD) and Therapeutic Targets Database (TTD). The targets of carthamin screened by the two websites and disease targets selected from the three databases were matched to screen the targets of carthamin against sepsis. The anti-sepsis potential targets of carthamin were identified by molecular docking software.Results:The oral bioavailability of carthamin was 41.15%, the drug-likeness was 0.24, and the rotational bond number was 1, which indicated that carthamin was well absorbed by oral administration and showed good drug formation. A total of 115 potential targets of carthamin were screened by SwissTargetprediction and DRAR-CPI; 149 disease targets were found from OMIM, CTD and TTD databases; 115 target proteins of carthamin screened by the two websites were matched with the disease targets , and 10 target proteins were found to be both molecular targets and disease targets. The 10 target proteins were coagulation factor Ⅸ (F9), adenosine A1 receptor (ADORA1), nitric oxide synthase 2 (NOS2), mitogen activity protein kinase 1 (MAPK1), cathepsin G (CTSG), neutrophil elastase (ELANE), protein C (PROC), lipocalin 2 (LCN2), glucose-6-phosphate dehydrogenase (G6PD) and prostaglandin endoperoxidase 2 (PTGS2). Molecular docking software analysis showed that carthamin had the ability to bind to the above 10 target proteins, which were potential targets of carthamin against sepsis. Carthamin could interact with the key amino acid residues of the targeted proteins, so as to play the corresponding efficacy.Conclusion:Carthamin combines with the targets could reduce the tissues and organs damage of sepsis by regulating CTSG, ELANE and LCN2, reduce inflammatory response of sepsis by regulating ADORA1, PTGS2, NOS2, MAPK1 and mediating PROC and F9 to inhibit clotting, and improve oxidative stress, reduce the incidence of sepsis by regulating G6PD, finally, prevented and treated sepsis.

Objective To explore the value of CT in diagnosing abdominal leiomyosarcoma.Methods CT findings of abdominal leiomyosarcomas conformed pathologically in 9 patients were analyzed.Results The tumors localized in peritoneum in 3 cases,in stomach in 3 cases,in rectum,colon and uterus in 1 case respectively.On plain CT scan,the tumors appeared as unhomogeneous dense masses with mecrosis at the centre,the periphery of tumors was flocculation or ringlike,and having fluid-fluid level in 1 case.The delayed peripheral enhancement on contrast CT could be seen.Conclusion CT imaging can help to diagnosis the abdominal leiomyosarcoma.

Objective To explore the short-term clinical outcome of percutaneous posterior lumbar interbody fusion using B-twin stand-alone expandable spinal spacer in the degenerative lumbar diseases.Methods From January 2006 to March 2009,41 consecutive patients with lumbar degenerative disc diseases were enrolled and 44 intervertebral spaces were observed in this study.There were 20 males and 21 females,with an average age of 57.51(ranging from 31 to 77 years old).Indications for treatment included degenerative lumbar disc herniation(LDH) with intervertebral instability or Ⅰ? spondylolisthesis(25 cases),LDH with intervertebral space collapse(15 cases) and lumbar discogenic pain(1 case).Fusion segments included 3 cases of L3,L4 and L5,3 cases of L3 and L4,27 cases of L4 and L5,and 8 cases of L5 and S1.After percutaneous discectomy and endplate,curettage were meticulously carried out.A B-twin expandable spinal spacer was introduced into the intervertebral space and then expanded and an allograft cancellous bone of 4 to 5 g was implanted around the B-twin in the intervertebral space.The whole operation was monitored by C-arm fluoroscopy.The clinical outcome was evaluated by JOA scale and X-rays and CT examination before the operation,and 1 week,3 and 12 months after the operation.Results After follow-up of 3 to 38 months(mean 16 months),all the patients were evaluated,and excellent was obtained in 13 cases,good in 21 cases,fair in 5 cases,and poor in 2 cases.The excellent and good rate was 82.93% according to the JOA scale.X-rays and CT showed that the subsidence of the B-twin was found in 3 cases,and 1 case was significantly subsided by more than 20%.Cage shift was seen in 1 case.Conclusion Percutaneou posterior lumbar interbody fusion with B-twin is efficient with minimal surgical trauma,blood loss,better rehabilitation and reliable short-term outcome.Strict indications and pinpoint skills for surgery are the precondition to obtain satisfactory outcome in the treatment of degenerative disc disease.

ObjectiveTo investigate the effects of erythro po ietin on prevention hypoxic-ischemic brain damage (HIBD) and activation of Casp ase-3 in Hippocampal CA1 region in newborn rats. Methods7 d Sprague-Dawley rats were divided into hypoxic-ischemic (HI) group (n=11), recombinant human erythropoietin (rhEPO) treated group (n=11), and sham-op erated control group (n=9). HIBD was established in both HI group and rhEPO treated group. The number of rats animals with spontaneous left-turn in two gro ups was counted respectively at subsequent different time: 0, 6, 12 and 24 h. Th e expression and distribution of activated Caspase-3 was detected by immunohist ochemistry analysis. The positive cells were calculated in hippocampal CA1 regio n of every groups.ResultsTwo rats in HI group and one in rhE PO treated group died from continuous convulsion during hypoxia. all survival ra ts in up two groups had spontaneously left-turn Compared with HI group, the r ate of spontaneous left-turn was dramatically lower in rhEPO treated group (HI group vs rhEPO treated group, 1/10 vs 6/9, P=0.0198) at 24 h after hypox ia. The positive stained cells were distributed dispersively in the brain of con trol group, and more intensively in the hippocampus and cerebral cortex of the o ther two groups. In CA1 region, the number of positive cells in HI group, was si gnificant higher than that in control group ( 41.38 ?2.09 vs 10.52?2.70 , P