Objective To investigate the expression of an inhibitor gene of apoptosis, clusterin, in prostate cancer and its relationship with the genesis and progression of prostate cancer and with the expressions of bcl 2 and p53. Methods The expressions of clusterin, bcl 2, and p53 in 10 cases of normal prostatic tissues, 15 cases of benign prostatic hyperplasia (BPH), and 49 cases of prostate cancer were examined by immunohistochemical staining. Results The rates of strong positive or weak positive of clusterin in normal prostatic tissues, BPH, and prostate cancer were 10% (1/10), 66.6% (10/15), and 91.8% (45/49), respectively. The expression level of clusterin in prostate cancer tissues was significantly higher than that in normal prostatic tissues ( P

The article summarizes the questions of clinical teaching of advanced urologists,and find out some measures to improve the clinical teaching quality of advanced urologists.

Objective To investigate the characteristics of V3 loop amino acid sequences of human immunodeficiency virus type 1 (HIV-1) quasi-species in long-term non-progressors (LTNP)infected with HIV. Methods End-point limiting dilution polymerase chain reaction (EPLD PCR) was used to amplify the env gene c2-v3-c3 region of single HIV-1 provirus from five LTNPs at sequential time points. The PCR products were then sequenced and the amino acid sequences of V3 loop were analyzed by sequence confirm analysis technology. Results The results showed that there were one to ten kinds of polymorphisms in the V3 region of HIV-1 quasi-species which were found from the serial samples of the five LTNP. However, the sequences of the predominant strains were either completely consistent or at most changed at one or two residues in the serial samples of individual patient. The tetramer compositions of the tip of V3 loop were consistent in each patient. It was GPGR in four patients and GPGK in one patient. It was speculated the co-receptor of HIV-1 was CC chemokine receptor (CCR)-5 based on the amino acids at the residue 11 and residue 25 of V3 loop and the net charge of V3 loop. Conclusions There are various polymorphisms at the HIV V3 loop in LTNP. However, the tetramer composition of the tip part of V3 loop is stable. The LTNP are very likely infected with non-syncytium inducing (NSI) strain.

detection of HIV-1 quasispecies in HIV-1 infected populations with low level viral load.

Medical clinical education is really the beginning of clinical work,and it should be reformed accordingly based on actual changes in health care.This article discusses that the clinical medical education should be reformed to face serious medical problems.

Along with contemporary development of life support technology in perinatology and neo-natal intensive care,mortality of preterm infants has been reduced.However,the problems caused by the brain damage have been attracting more and more attention.The long-term outcome of prematures depends on their nervous system sequelae among the total complications.Preterm infants'brain damage mainly includes intravent-ricular hemorrhage(IVH)and periventricular leukomalacia(PVL).IVH and its complications are the main causes of early death,mental and physical developmental disorder of neonatals.Thus,the early diagnosis of brain dam-age,especially PVL,has become a serious problem that we are going to face.The scholars in or aboard hao payed close attention to these markers,such as MBP、S100B、ACT A、NF-L、NGB、MMP、IL-6、IL-10、IL-1 1 、NSE.The paper reviews these biological markers.

Purpose To propose a novel compression method for region of interest (ROI) based on Curvelet transform and SPIHT algorithm. Materials and Methods The ROI was firstly extracted without compression, and Curvelet transform was applied for the background regions. The Curvelet coefifcients were coded using SPIHT algorithm. Then the images after compression are obtained by inverse Curvelet transform. The ROI and the background were ifnally overlapped to get the full compressed image. Effect of ROI compression and overall compression were compared, as well as the Curvelet transform and wavelet transform, based on peak signal noise ratio. Results The ROI compression highlighted the region of interest and the visual effect was superior to the overall compression. The peak signal to noise of Curvelet transform was higher than that of wavelet transforms, and the compressed images were more clear for the same proportion. Conclusion ROI compression based on Curvelet transform and SPIHT algorithm can achieve efficient compression images without losing important diagnostic information, which complies with the requirement of high precision and high quality of medical image compression.

Objective To determine the relationship between the content of microelement and the health of children.Methods Atomic absorption spectrometry,aas.Children were divided into 3 groups:physically weak group,minimal brain dysfunction(MBD) group and control group.Peripheral blood gathered from all the children.Put 40?l of blood sample into the specific reagent,detect the content of zinc(Zn),cuprum(Cu),ferret(Fe),calcium(Ca),magnesium(Mg),lead(Pb),cadmium(Cd).The result was compared between the 3 groups.Results Lack of Zn,Fe,Ca and Mg mostly be detected in physically weak group(P

Objective To investigate the effects of Naoluoxintong which is representative prescription of supplementing Qi and activating blood therapy(Yiqihuoxue),the prescription of supplementing Qi therapy(Yiqi) and the prescription of activating blood therapy(Huoxue) on local cerebral ischemia-reperfusion injury rats.To observe the changes of neural stem cells(NSCs) and correlated SVZ factors.Methods Models of local cerebral ischemia-reperfusion were established by thread-occluding the MCA.The time points were observed at 1,3,7 d after reperfusion following 2 h of MCAO respectively.The expression levels of Nestin were measured by immunohistochemical staining.Results The expression of Nestin of treatment groups increased significantly compared with model group at 7 d(P

Objective To explore the clinical manifestation of stroke caused by central nervous system vasculitis (CNSV). Methods 49 patients who suffered from stroke by CNSV were reviewed retrospectively. Results Among the 49 patients, 5 were intracerebral hemorrhage, 15 were ventricular hemorrhage, 7 were subarachnoid hemorrhage and 22 were cerebral infarction; the mean of age was (26.0±12.7) years old. Conclusion The clinical manifestation of stroke caused by CNSV is variform, and usually happens in young age. CNSV should be considered as the cause of stroke in adolescent.