Objective To investigate the relationship between homocysteine(Hcy)and blood uric acid(UA)in the patients with type 2 diabetes mellitus(T2DM)complicating hypertension.Methods 85 patients with T2DM complicating hypertension were se-lected as the groupⅠ,78 patients with simple T2DM as the group Ⅱ and 74 individuals with healthy physical examination as the group Ⅲ.Hcy,UA,TC,TG and HDL-C were measured in all the subjects.Results Compared with group Ⅲ(control),the levels of Hcy,UA,TC and TG in the group Ⅰand groupⅡwere significantly increased(P <0.05),but the level of HDL-C was significantly decreased(P <0.05).Compared with the group Ⅱ(T2DM),the levels of Hcy,UA,TC and TG in the group Ⅰwere significantly in-creased(P <0.05 ),but the level of HDL-C was obviously decreased,the difference had statistical significance (P <0.05 ).There was good positive correlation between the Hcy level and the UA level in the patients with T2DM complicating hypertension (r =0.658,P <0.05).Conclusion The levels of Hcy and UA are significantly increased in the patients with T2DM especially complica-ting hypertension.High Hcy and high UA are the important risk factors of T2DM complicating cardiocerebrovascular diseases.

Actinomycosis ; pathology ; Adult ; Diagnosis, Differential ; Female ; Humans ; Mycoses ; pathology ; Staphylococcal Skin Infections ; microbiology ; pathology ; surgery ; Staphylococcus aureus ; isolation & purification ; Young Adult

Results of experiments such as ammonia steaming test in mice and citr ic acid test in guinea pigs, phenol red secretion test in mice and capillary exp ectorant test in rats, in-vivo and in-vitro antiasthmatic tests in guinea pigs p roved that Tankeqing capsule had good antitussive, expectorant and antiasthmatic effects and a significant time-effect relationship was showed. The antitussive effect and expectorant effect arrived to the peak in 1-6h after oral administrat ion, and the antiashmatic effect in about 1h.

Results of experiments such as ammonia steaming test in mice and citric acid test in guinea pigs, phenol red secretion test in mice and capillary expectorant test in rats, in-vivo and in-vitro antiasthmatic tests in guinea pigs proved that Tankeqing capsule had good antitussive, expectorant and antiasthmatic effects and a significant time-effect relationship was showed. The antitussive effect and expectorant effect arrived to the peak in 1-6h after oral administration, and the antiashmatic effect in about 1h.

Objective To investigate the effect of Shuyusan on the behavior of chronic stress induced depression model rats, as well as concentrations changes of norepinephrine (NE), dopamine (DA) and serotonin (5-HT) in cerebral cortex. Method Fifty mice were randomly divided into 5 groups:normal control group, model group, amitriptyline group, and high/low dose prescription group of Shuyusan. Except normal group, the other groups were given the chronic mild unpredicted stress for 21 days. After 21 days different stimulation, open-Field test was used to estimate the behavior of rats, the concentrations of NE, DA and 5-HT were detected with fluorescence spectrometer method. Results Horizontal and vertical motion of the model group was significantly lower than that of the control group (P

Objective To investigate the influences of 5-azacytidine (5-Aza) with different concentrations on mesenchymal stem cells (MSCs) proliferation and differentiation into myoblasts. Methods Bone marrow-derived MSCs of 4 weeks Wistar rats were separated and purified, and then treated with 5-Aza with different concentrations. The growth ability of cell was assayed with methyl thiazolyl tetrazolium (MTT) method. The expression of skeletal muscle actin in MSCs was determined with reverse transcription polymerase chain reaction (RT-PCR) and electrophoresis after MSCs were induced. Results The cell proliferation was not affected by 0 and 1 ?mol ? L-1 5-Aza . There were expressions of skeletal muscle actin treated with 3 -12 ?mol ? L-1 5-Aza. Some cells were obviously enlarged at the 9th day after induction and myotubu-like cells were found at the 12th day when treated with 9 and 12 ?mol ? L-1 5-Aza. 20 - 30 ?mol ? L-1 5-Aza induced the toxic effect on proliferation of MSCs. With the increase of concentration, the proliferation ability of MSCs was weakened. Conclusion 5-Aza affects the expression of regulatory gene to the stem cells and regulate MSCs to orientationally differentiate into myotube-like cells.

Objective To develop a balloon-type head fixation pillow for interventional neuroradiology operation.Methods Some radiotransparent sponge with high elasticity and strength and some nonopaque cloth with high skin compatibility were employed to make pillow base adaptable to the posterior fossa, neck and shoulder. A inflatable balloon made of anti-tensile airtight nylon was used to fix the underjaw.Results The fixation pillow gained advantages over the common one in imaging times, X-ray exposure dose and abnormal condition.Conclusion The head fixation pillow may decrease operating time, exposure dose and complications during interventional neuroradiology operation, and thus is worth popularizing clinically.

Objective To explore the exercise induced immunity changes during incremental load training from the view of the expression of helper T cells (Th) mRNA. Methods Using the technique of real-time quantified PCR to observe T-helper-special cytokine mRNA of peripheral blood mononuclear cell dynamically in healthy young male who have been trained with incremental exercise for five weeks. Results There was no obvious change in IL-2 mRNA during five weeks training. Meanwhile IL-4 mRNA increased greatly in the second, third and fourth week. IFN-? mRNA as well as IFN-? mRNA/IL-4 mRNA increased remarkably in the third, fourth and fifth week . Conclusion The expression of T-helper1/ T-helper2 cytokine mRNA were up-regulated after five weeks and the subjects were gradually adapt to the increased load which indicated the enhancement of immunity function .

Objective To construct prokaryotic recombinant expression plasmid carrying Pneumocystis carinii Mr 55 000 antigen(p55) gene fragment and express the recombinant protein. Methods P. carinii pneumonia(PcP) rat models were established by subcutaneous injection of dexamethasone for 14 weeks. Total RNA was extracted from lung of P. carinii rat and p55 antigen gene fragment was cloned by RT-PCR,which was identified by sequencing. The 690 bp fragment was cloned to pGEX-4T-1,the recombinant plasmid was screened and identified by restriction analysis and PCR. The recombinant plasmid was finally induced with IPTG to express a new fusion protein,and the products were analyzed by SDS-PAGE and Western blot. Results A fragment of 690 bp was obtained by RT-PCR. The recombinant pGEX-4T-1/690 was constructed. SDS-PAGE revealed that the molecular weight of the recombinant protein was approximately Mr 62 000,the maximum amount of the fusion protein produced was 11.6% of the total protein. The recombinant protein can be recognized by GST antibody and by the sera from P. carinii infected rats using Western blotting. Conclusion Prokaryotic expression plasmid pGEX-4T-1/690 has been constructed and the recombinant fusion protein shows antigenicity.