1.Determination of 39 Polybrominated Diphenyl Ethers in Coastal Sediments by Ultrasonic Extraction Coupled with Gas Chromatography-Mass Spectrometry
Chinese Journal of Analytical Chemistry 2009;37(11):1627-1632
A method was developed for the determination of 39 polybrominated diphenyl ethers(PBDEs) from mono- to hepta-brominated in coastal sediment by gas chromatography/mass spectrometry. The sample was extracted with a solution of dichloromethane: n-hexane( 1:1,V/V) ,followed by ultrasonic extraction for 60 min at 25℃. The clean-up step was optimized using silica gel and alumina. The detection limit of the method was in the range of 0.003 - 0.10μg/kg. The results indicate that the average recovery of the internal standard was from 66.2% to 118. 6%. The relative standard deviation RSD( % ) was from 0. 8 % to 18. 2%. The method for the detection of 39 PBDE congeners in coastal sediment was sensitive,high reproducible with satisfactory recoveries.
2.Artificial liver support system used in the perioperative period of liver transplantation
Tao JIANG ; Gang CHEN ; Weiwei JIANG
Chinese Journal of Tissue Engineering Research 2007;0(31):-
An artificial liver support system (ALSS) is a body circulatory system aims to blood purification, which consists of plasma exchange, hemodialysis, hemoperfusion, in combination with hemodiafiltration. The clinical application of non-biological artificial liver, especially plasma exchange technology, can solve the problem such as hyperbilirubinemia, endotoxemia, toxic substance accumulation and disorder of amino acid metabolism, which would save patients’ lives. ALSS can significantly improve the general physical condition, reduce operative risk, as well as relieve physiologic derangement after liver transplantation. Therefore, artificial liver serves as a bridge for liver transplantation. This paper analyzes the characteristics of ALSS, explores the methods of ALSS application in perioperative period of liver transplantation, and discusses its developing orientation.
3.Formation and characteristics of crystals on the surface of mineral trioxide aggregate in vitro
Wenxia CHEN ; Bing FAN ; Tao JIANG
Journal of Practical Stomatology 2001;0(03):-
Objective:To examine the surface of mineral trioxide aggr eg ate (MTA) when treated in different conditions in vitro. Method: Root blocks were prepared. MTA and other three dental materials (Dycal, Dyr act and amalgam) were respectively filled into the root canals of prepared root blocks. The roots with the materials were maintained in distilled water or simul ated body fluid (SBF) at 37 ℃ for 5 days. Scanning electron microscopy(SEM) and X-ray energy dispersive spectroscopy(EDS) were employed to investigate the mor phological changes and chemical components. Results:There was pr ecipitate of simple crystal units or crystalline structure in the MTA specimens. EDS showed one or two peaks corresponding to calcium or calcium and phosphorus. Conclusions:Apatite may produce and grew by MTA on its surface.
4.Role of SLB elements presented at 5 UTR of dengue virus on translation and RNA synthesis
Xuedong YU ; Tao JIANG ; Shuiping CHEN
Medical Journal of Chinese People's Liberation Army 2001;0(07):-
Objective To investigate the effects of SLB elements presented at 5' UTR of dengue virus genome on viral translation and RNA replication.Methods The 5 end of RNA secondary structures of dengue virus genome were predicted using mfold 3.2,and three mutants with different modifications in SLB elements were designed:SLB1,part deletion of the 5' UAR sequence(82-87nt);SLB2,mutation of the sequence on stem(Mut 77G/C);SLB3,mutation of the sequence on stem(M77-78AG/GA),respectively.The mutants described above were constructed by OL-PCR based on DEN-R.luc2A-RP,respectively.Replicon RNAs corresponding to DEN-R.luc2A-RP,DEN-R.luc2AGDD-RP and the 3 mutants mentioned above were in vitro transcribed and equal amounts of RNA were transfected into BHK cells with Lipofectamine 2000.After RNA transfection,the replicons were detected and characterized by RT-PCR,IFA,Renilla Luciferase assay system and real time RT-PCR,respectively.Results It was shown that SLB1 mutant did not significantly affect the translation of the input RNA,but seriously compromised RNA synthesis;SLB2 mutant did not significantly affect the translation of the input RNA either,but its RNA replication was abolished;both the translation and replication of SLB3 mutant were abolished.Conclusion Both the nucleotide sequence and the RNA secondary structure of the second loop and short stem of SLB element are highly conserved.SLB element may play an essential role on viral translation and RNA replication.The findings of present study may set a foundation for elucidating the role of SLB during viral translation and replication.
5.The cilinic discussion of 120 the female condyloma acuminatum cases to cure with the microwave adds ?-interferon
Yaomu JIANG ; Tao LU ; Youren CHEN
Chinese Journal of Primary Medicine and Pharmacy 2006;0(06):-
Objective To observe the microwave adds the curative effect and function mechanism of the female condyloma acuminatum treatment in ?-interferon treatment.Methods 120 cases of condyloma acuminatum were treat and compared with 120 cases with the microwave treatment with the microwave and ?-interferon.Results Two clinical cure rates made no difference,relapsing after curing the rate compare,the treatment set was 2.67%,the matched control was 31.54%.It had obvious difference(P
6.Operating Principle and Troubleshooting of Data Acquisition Section of TOSHIBA Aquilion 16
Yi CHEN ; Tao JIANG ; Liqi XIE
Chinese Medical Equipment Journal 2004;0(08):-
The operating principle of data acquisition section of TOSHIBA Aquilion 16 is introduced.The operating principles of the main detector,the reference detector,the data acquisition section and its power supply are analyzed.The troubleshooting methods for DAS are put forward.
7.Multiple primary cancer of digestive tract
Youheng XIA ; Xuezhong CHEN ; Tao JIANG
Chinese Journal of General Surgery 2001;10(3):250-252
Objective To study the clinical features, diagnosis and treatment of multiple primary cancer of digestive tract (MPCDT). Methods The clinical data of 33 cases of MPCDT were analysed retrospectively. Results The incidence of MPCDT was 2.7% in all patients with primary cancer of digestive tract. 24 cases was synchronous carcinoma (SC), of whom, 20 was misdignosis. All the cases of SC received comprehensive treatment, including radical resection, traditional medicine and radiotherapy respectively. 9 cases were metachronous carcinoma (MC), of them, only 1 case misdiagnosed. All the MC cases received radical resection, palliative resection and radiotherapy. The 5-year survival rate in cases of MPCDT, SC and MC were 45.4%, 37.5% and 55.5% respectively. Conclusions The incidence of MPCDT is low, but misdiagnosis rate is hight. For MPCDT, comprehensive treatment, and radical resection and re-operation should be done. It is advisable not to diagnose MC as recurrence or metastasis.
8.Role of expression of protein kinase C in spinal dorsal horn in metabotropic glutamate receptor 5's participation in development of morphine tolerance in rats
Moxi CHEN ; Tao XU ; Wei JIANG
Chinese Journal of Anesthesiology 2010;30(9):1048-1050
Objective To evaluate the role of the expression of protein kinase Cγ and Cα in spinal dorsal horn in the metabotropic glutamate receptor subtype 5 (mGluR5)'s participation in the development of morphine tolerance in rats. Methods Thirty-two male SD rats in which the intrathecal (IT) catheter was successfully placed were randomly divided into 4 groups (n = 8 each): control group (group C), morphine tolerance group (group M), antisense oligodeoxynucleotide (ODN) plus morphine group (group ANT) and mismatch ODN plus morphine group (group MIS). Rats in group M received 0.9% normal saline (NS) 5 μl twice a day for 8 days, and IT morphine 15 μg was injected simultaneously at 6, 7 and 8 days twice a day. Rats in group ANT and MIS received IT antisense and mismatch ODN 30 nmol (in 0.9% NS 5 μl) twice a day for 8 days respectively and IT morphine 15 μg was injected simultaneously at 6, 7 and 8 days twice a day. Rats in group C received NS instead twice a day for 8 days. Paw-withdrawl threshold (PWT) to thermal and mechanical stimulation was measured at 6, 7 and 8 days after ODN injection (T1-3). The animals were killed on 9th day (T4) and the lumbar segment of the spinal cord was removed for determination of the expression of mGluR5, PKCα and PKCγ mRNA (by RT-PCR), PKCαand PKCγ ( by Western blot). Results PWT to thermal and mechanical stimulation was significantly higher at T1.2in group M and MIS and at T1.3 in group ANT, the expression of mGluR5 mRNA, PKCα and PKCγ was significantly higher at T4 in group M and MIS, and mGluR5 mRNA expression was significantly lower at T4 in group ANT than in group C ( P < 0.05). PWT to thermal and mechanical stimulation was significantly higher at T2.3, while the expression of mGluR5 mRNA, PKCα and PKCγ lower at T4 in group ANT than in group M ( P < 0.05). Conclusion The expression of protein kinase C in spinal dorsal horn plays an important role in the mGluR5's participation in the development of morphine tolerance in rats.
9.Preparation and targeting evaluation of reductant-sensitive oxaliplatin-loaded Fe3O4 nanoparticles
Yu WANG ; Qin-jun CHEN ; Tao SUN ; Chen JIANG
Acta Pharmaceutica Sinica 2022;57(1):188-199
The non-specific accumulation and release of drugs are the main factors affecting the therapeutic effect as well as causing toxic side effects of chemotherapeutic drugs. Nowadays, the application of nanotechnology and responsive drug release is an important strategy to improve the tumor-specific accumulation of drugs and reduce their side effects. In this study, an
10.Ultrastructural changes of the retina after ocular blast injury in rabbits
Suihua CHEN ; Chunyan XUE ; Tao JIANG ; Hui ZHANG ; Yingjuan ZHENG ; Zhongcui JIANG ; Shaojun JIANG
Journal of Medical Postgraduates 2004;0(02):-
Objective: To investigate the ultrastructural pathogenesis of retina injury by observing the ultrastructural changes under the transmission electron microscope(TEM) after ocular blast injury in rabbits.Methods: Ocular blast injury models were set up in 20 rabbits by the bow wave produced with a bioshock tube.The rabbits were sacrificed at scheduled times after injury,their retinas obtained and their ultrastructural changes observed by TEM.Results: The axonal ultrastructural changes of the retina induced by blast were summarized as follows.The microfilaments and microtubules were swollen and distorted in the early stage,followed by reactive swelling of the ganglion cells.The swollen mitochondria and endoplasmic reticula focally accumulated and the cytoskeleton was destroyed.Finally the intraaxonal cellular structure disappeared and the axon disconnected.Conclusion: Ocular blast injury may cause retinal ultrastructural changes.The pathological changes of ganglion cells in the optic nerve may be associated with the direct effect of the blast and/or ischemia and are possibly important factors in the pathogenesis of vision disturbance.