The changes of hemodynamics and hypoxic pulmonary vasoconstriction (HPV) inducedby cigarette smoking and the role of prostaglandins(PGs) and leukotrienes (LTs) were studied in rats. During smoking, systemic and pulmonary vascular resistance (SVR and PVR) increased 47% and 39%, respectively. The level of TXB_2 rose by 144% in plasma and 69% in lung tissue. HPV increased two-fold after smoking (△PVR increased from 55% to 102%). LTs receptor antagonist, LY-171883, prevented the smoking-in duced increase of SVR and PVR and augmentation of HPV. Cyclooxygenase inhibitor, in domethacin, prevented partly increase of PVR but not HPV induced by smoking. Our results suggest that smoking leads to pulmonary and systemic vasoconstriction and augmentation of HPV mediated by LTs, the increase of PVR during smoking is also mediated by TXA_2, This study also found that LY-171883 inhibited HPV by 72%, the result indictates that LTs mediate HPV in Wistar rats.

In this paper, the role and significance of adaptive sports on rehabilitation education of special children were redefined, and the concept and modules of adaptive sports course were introduced, in order to provide a theoretical reference for adaptive sports course of the special school under the theory of International Classification of Functioning, Disability and Health for Children and Youth.

Tumor tissue hypoxia is taken as an important biologic character of malignant tumors,it could reduce the therapeutic efficacy of radiotherapy and chemotherapy. Presenting a noninvasive approach in detecting hypoxic state in tumors,hypoxia imaging of nuclear medicine can be used in the differential diagnosis,prognosis evaluation and guiding therapy of malignant tumors.

In order to cultivate students' quality of scientific research thinking,self-learning ability,innovation ability and practical ability,Open Innovation Experiment Project which is suitable for the undergraduate students has been carried out in Jilin university.The feature of experiment is based on the teacher's scientific research project,and the experimental design is completed by the students independently.By the stage inspection as a monitoring mechanism,the overall effect evaluation of the experimental project has been conducted by the combination of the process and the results.Through the selection,design,implementation and results of practice,students can not only cultivate innovative consciousness and comprehensive thinking and the spirit of unity and cooperation,but also have a basic understanding of scientific research work.

Objective To study the relationship between polymorphic (CAG)n repeats of androgen receptor(AR) gene and occurrence of prostate cancer (PC). Methods (CAG)n repeats of both malignant cells and adjacent nonmalignant cells from 34 paraffin embedded PCs, and that of peripheral blood cells from 2 PC patients were assessed using dsDNA cycle sequencing. Results The number of (CAG)n repeats in malignant cells was equal to that in adjacent nonmalignant cells from the same paraffin embedded PC; the mean of (CAG)n repeats in the 36 PC patients was 20.06 which was significantly smaller than that of normal men ( P 0.05). Conclusions Shortening of (CAG)n repeats within AR gene may be associated with the occurrence of PC.

Purpose To improve the understanding of features and diagnostic value of PET/CT imaging in primary tracheal cancer as it is a rare disease.Materials and Methods The18F-FDG PET/CT image data of 11 patients with primary tracheal cancer confirmed pathologically were retrospectively analyzed. The contrast-enhanced CT scan was performed in 9 patients. The PET/CT and contrast-enhanced CT imaging data were analyzed.Results Out of the 11 cases, 5 were adenoid cystic carcinoma, 4 were squamous cell carcinoma and 2 were adenocarcinoma. Endotracheal round or elongated uptake lesions were found in PET imaging. The maximum standardized uptake value of adenoid cystic carcinoma, squamous cell carcinoma and adenocarcinoma were 4.5±1.2, 8.1±1.7 and 4.5±2.4, respectively. The accuracy of diagnosis of PET/CT was 100%(11/11). Soft tissue density nodule or mass in trachea was found in CT scan. The accuracy of CT diagnosis was 81.8% (9/11). Among the 9 patients who underwent enhanced CT scan, 3 had significantly enhanced lesions and 6 had moderate enhanced ones. Mediastinal lymph node metastasis was found in 1 patient. There was no distant metastasis in all the 11 patients.Conclusion Primary tracheal cancer has features of PET/CT imaging such as soft tissue nodules or mass in tracheal lumen or on its walls accompanied by FDG increase. The utilization of metabolic and anatomic imaging of PET/CT has great significance in clinical diagnosis and therapy of primary tracheal cancer.

This article discusses how to use teaching platform provided by information technique to integrate information technique with clinical teaching of college courses,strengthen material collection in clinical teaching and train students' thinking and cognitive ability.

Objective To investigate the possibility of measuring ABO blood group antibody levels using renal cortical tubular epithelial cells (RCTECs) of cynomolgus monkey.Method The primary RCTECs were isolated from cynomolgus monkey kidneys and identified by Western blotting and flow cytometry (FACS).FACS was applied to detect the expression of ABO histo-blood group antigens on RCTECs,the binding of blood group antibodies in human serum to RCTECs,and to compare the difference of measuring ABO blood group antibody levels between using human erythrocytes and RCTECs as target cells.Result The majority of cells derived from the kidney cortex were renal tubular epithelial cells.39.90％-73.80％ of RCTECs were found to express the ABO histoblood group antigens with the capability to bind the human blood group antibodies specifically.The expression level of ABO histo-blood group antigen on RCTECs was not influenced by long-term cryopreservation,and the percentage of B antigen positive cells was 39.90％ before frozen storage and 39.65％ after a 10-month cryopreservation respectively.The normal serum and allergic serum presensitized to synthetic A antigen-keyhole limpet hemocyanin (KLH-A) of cynomolgus monkey were used to compare the abilities of RCTECs and human erythrocytes to detect antibody titers.No agglutination was observed in RCTECs test group.The mean fluorescence intensity (MFI) values of antibody were highest when the dilution fold was lowest (1 ∶ 16) and gradually decreased with increased serum dilution in both serum groups.MFI fell towards baseline value at 1 ∶ 128 dilution in normal serum group while at 1∶8 192 dilution in allergic serum group.Between 1 ∶ 16 and 1∶8 192 dilutions,MFIs of normal serum group were all lower than those of allergic serum.In human red cells test group,obvious agglutination appeared at high concentrations of antibodies,and MFIs reached the peak at 1∶64 dilution in normal serum group and at 1∶32 dilution in allergic serum group,but fluctuated irregularly thereafter.Between 1∶64 and 1∶512 dilutions,MFIs of normal serum group were all higher than those of allergic serum.Data above showed that MFI values in RCTECs group could reflect the levels of blood group antibodies more exactly.Conclusion The RCTECs of cynomolgus monkey express ABO tissue-blood group antigens and would be more suitable to be used to measure ABO blood group antibody levels by FACS.

As a novel Golgi protein,GOLPH2 expression is associated with maintaining the structural integrity of the Golgi apparatus.As an oncoprotein,GOLPH2 expression is associated with malignant biological behavior of cancer cells.Important roles of GOLPH2 in the tumorigenesis and metastasis make it become a new target for tumor diagnosis and treatment.

With growing dependence on picture archiving and communication systems(PACS) for viewing images,a quality assurance program to the condition of workstation displays and image quality guidelines for better cost-efficacy and diagnostic accuracy has become increasingly important.This article analyses and synthesizes the quality control of the PACS phantom workstation mainly from such aspects as the monitor resolution and brightness,the environment degree of illumination,statistics assessment method(ROC curve) and so on.