Objective To compare the clinical efficacy of intraspinal tumor resection under quadrant channel and hemilaminectomy surgery.Methods We retrospectively studied 33 patients with intraspinal tumor with the lesion length of less than 5 cm hospitalized in our hospital from July 2013 to July 201 5.Of the 33 patients,1 6 patients received intraspinal tumor resection under quadrant channel and 1 7 patients received intraspinal tumor resection under hemilaminectomy surgery.We compared various indicators in the two groups,including the length of surgical incision,surgical operation time,bleeding volume during operation,time for the first ground activity after surgery,length of hospital stay (LOS)after surgery,visual pain simulation (VAS)score 1 week after surgery, and JOA score excellent and good rates.Results The patients who received quadrant channel intraspinal tumor resection outperformed those who received hemilaminectomy surgery regarding the length of surgical incision, bleeding volume during operation,time for the first ground activity after surgery,LOS after surgery,and VAS score 1 week after surgery (P <0.05 ).Conclusion Intraspinal tumor resection under quadrant channel is a safe and effective therapy because of small trauma,less bleeding,quick recovery and curative effect.

Objective To investigate the effects of low molecular weight polypeptide(LMP) extracted from chicken embryo on learning and memory in senile mice induced by D-galactose(Gal). Method The 16 d old chicken embryo was acid and alkali hydrolyzed,enzymolyzed and ultrafiltered to formulate LMP. 48 mice were divided randomly into four groups: control,aging model ,aging+low dose LMP,aging+high dose LMP. The aging model and two LMP groups were treated with Gal 80 mg/ (kg bw?d) by nape subcutaneous injection,while the control group with normal saline 8ml/(kg bw?d). Control and model groups were given i.g. normal saline 20 ml/(kg bw?d),and two LMP groups 10 ml or 20 ml/(kg bw?d) LMP respectively. Learning and memory of mice were tested with Morris water maze. Results The escape latency of model group was longer and the percentage of swimming distance in platform region higher than that of control group (P

Objective To investigate the role of Ki-67,c-myc,p53 in human in esophageal cancer.Methods 25 specimens of human esophageal cancer as observe group and 25 adjacent normal specimens as control group were selected.Expressions of Ki-67,c-myc,p53 protein were detected by immunohistochemical staining.Results Protein expressions levels of Ki-67,c-myc and p53 in human esophageal cancer were significantly higher than those in control group 5.0,2.0,3.0 vs 1.0,0.5,0.3,respectively (all P ＜ 0.05).The expression level of Ki-67 protein in high differentiation group were significantly lower than that of moderate and low differentiation group (3.0 vs 6.0,P ＜ 0.05).In human esophageal cancer specimens,the expression level of Ki-67 protein was positively correlated with that of c-myc (P ＜ 0.05).Conclusion The expressions of Ki-67,c-myc and p53 are higher in esophageal cancer,which are increased with the higher differentiation.Ki-67,c-myc and p53 proteins may be taken as potential markers in human esophageal cancer.

Objective:To explore the feasibility and safety of laparoscopic radical gastrectomy for gastric cancer through analysising the laparoscopic radical gastrectomy and open radical gastrectomy.Methods:Retrieval the Cochrane Library,PubMed,EMBASE,CBM,VIP,CNKI,WANFANG DATA,the time is from January 2010 to August 2015.Bring into the comparative studies about laparosopic radical gastrectomy and laparotomye.Processing data using the revman 5.2 to reaserch the feasibility and safety of laparoscopic radical gastrectomy.Results:7 clinical comparative studies were included in this study.The observation group was the group of laparoscopic radical gastrectomy and the control group was the group of laparotomye.Results showed that the length of incision of the observation group was shorter[MD=-12.93,95％ CI (-13.16,-12.70),P＜0.00001];The observation group bleeding was lesser[MD=-129.98,95％C1(-220.54,-39.42),P=0.005];The number of lymph node of the two groups had no significant difference [MD=0.77,95％ CI (-2.56,3.60),P=0.56];The operation time of the observation group was longer [MD=49.17,95％ CI (25.32,73.02),P＜0.0001];The hospitalization time of the observation group is shorter [MD=-2.27,95％ CI (-3.77,0.77),P=0.003].Conclusion:Laparoscopic radical gastrectomy is feasible and it is superior to laparotomye within the thoroughness,minimally invasiving and safety.

Objective To investigate the effect of low molecular weight substance extracted from chick embryo on mitochondrial DNA(mtDNA)deletion in senile mice induced bv D-galactose.Methods Senile mice induced by D-galactose were treated with low molecular weight substance extracted from chick embryo.The deleted fragment of mtDNA was examined by using polymerase chain reaction technique and agarose gel electrophoresis.A relative quantitation of band densities was performed by using densitometry scanning techniques.The deletion was identified by using direct sequencing analysis. Results The 4239 bD mtDNA deletion were present in liver,cerebral cortex and hippocampus tissues in all of the mice.and it was significantly higher in senile model mice than in control mice(all P＜0.01).Low molecular weight substance extracted from chick embryo reduced the mtDNA deletion in various tissues in senile model mice(all P＜0.05).Deletion was more abundant in liver than in cerebral cortex and hippoeampus. Conclusions The 4239 bp mtDNA deletion is a common deletion,and low molecular weight substance extracted from chick embryo could decrease the incidence of 4239 bD mtDNA deletion.

ObjectiveTo investigate the dosage of protamine to counteract heparin in patients with different pH values of after - surgery plasma of congenital heart defect.MethodsThe clinical data of 108 patients during March 2011 to April 2011 with congenital heart diseases who underwent cardiopulmonary bypass(CPB) surgery were reviewed.The volumes of chest tube drainage were analyzed to investigate the dosage of protamine in patients with different pH values in plasma.ResultsThe dosages of protamine and the volumes of chest tube drainage[ ( 136.8 ± 22.8 ) ml] in patients with an acidic environmental plasma were higher than the patients in another group [ ( 112.6 ± 22.7 ) ml ] ( P＜ 0.01 ).In patients with non-acidic environments,the mean ratio of dosage of protamine to heparin was 1.23:1 ; meanwhile in patients with pH＜7.30 or base excess (BE) ＜ -6,the mean ratio was 1.86:1.It suggested the dosage of protamine increased significantly in patients with an acidic environmental plasma.ConclusionsDifferent plasma pH values could change the dosage of protamine after cardiopulmonary bypass,and the acidic environment would increase the dosage of protamine and increase the volume of chest tube drainage after surgery.When pH ＜ 7.30 or BE ＜ - 6 at the end of CPB,to correct acid-base balance first and then calculate the dose of protamine was recommended.

Objective To understand the therapeutic effect of clindamycin combined with compound sulfamethoxazole tablets on pneumocystis pneumonia(PCP)associated with acquired immunodeficiency syndrome (AIDS).Methods 97 AIDS patients with PCP in a hospital from January 2014 to March 2015 were randomly divided into control group (n=49,received compound sulfamethoxazole )and trial group(n=48,received clindamycin on the basis of com-pound sulfamethoxazole ),levels of partial pressure of oxygen in arterial blood (PaO2 ),arterial blood oxygen satu-ration(SaO2 ),serum albumin(ALB),and lactic dehydrogenase (LDH)in two groups of patients before and after treatment were recorded.Results Levels of PaO2 ,SaO2 ,ALB,and LDH between two groups of patients before treatment was not significantly different(all P >0.05).After treatment,PaO2 in control group and trial group were (73.01 ±4.62)mmHg and(84.92 ±5.34)mmHg respectively,SaO2 were (75.81 ±4.28)% and(90.86 ±5.94)%respectively,ALB were (32.62±4.41 )g/L and(43.95 ±5.03)g/L respectively,LDH were(416.53 ±30.77)U/L and(331 .58±20.86)U/L respectively,levels of PaO2 and SaO2 in trial group were both higher than control group , difference in ALB and LDH between two groups of patients after treatment were both statistically significant(both P <0.05).The total effective rate of trial group was 89.58% (n=43),which was higher than 69.39%(n=34)in control group (χ2 =6.04,P =0.014).Conclusion Clindamycin combined with compound sulfamethoxazole tablets has good therapeutic effect on AIDS and PCP,which is worthy of clinical popularization and application.

OBJECTIVE:To establish the quality standards of processed Sophora japonica.METHODS:Different batches of processed S. japonica products were detected in respect of property,microstructure,TLC,moisture,total ash,acid insoluble ash,extract inspection,content of total flavonoids and rutin. RESULTS:The limit value of test term for S. japonica,stir-baked S. japonica,S. japonica carbon were confirmed. CONCLUSION:Established standard is applicable for the quality control of processed S. japonica.

Objective To assess the effects of different statins in the prevention of contrast induced nephropathy (CIN)by comparing the changes of early renal injury biomarker serum levels in patients of with coronary artery angiography (CAG). Methods 183 patients who had normal renal function with CAG with normal renal function were randomly divided into the control group (n=61)、the atorvastatin group (n=60) and the rosuvastatin group (n=62). Renal injury biomarker levels (Scr,mALB, Cys C)and serum inflammatory factor (high sensitivity C reactive Protein,hs-CRP) of three groups were monitored and analyzed before and after CAG. Results The mALB(mg/L, 18.19 ± 8.25 vs. 12.07 ± 6.15,14.81 ± 6.23 vs. 12.32 ± 5.19,15.31 ± 7.53vs. 11.39 ± 5.38), Cys C(mg/L, 1.36 ± 0.32 vs. 0.90 ± 0.38,1.18 ± 0.25 vs. 0.91 ± 0.31,1.15 ± 0.21 vs. 0.93 ± 0.30), hs-CRP(mg/L,5.52 ± 2.09 vs. 1.96 ± 0.55, 3.86 ± 1.15 vs. 2.01 ± 0.34,3.93 ± 1.09 vs. 2.03 ± 0.23)levels in three groups raised up-regulated after angiography with statistical significance (P <0.05). Levels of mALB(mg/L,18.19 ± 8.25 vs. 14.81 ± 6.23, 18.19 ± 8.25 vs. 15.31 ± 7.53), Cys C(mg/L, 1.36 ± 0.32 vs. 1.18 ± 0.25,1.36 ± 0.32 vs. 1.15 ± 0.21), hs-CRP(mg/L, 5.52 ± 2.09 vs. 3.86 ± 1.15, 5.52 ± 2.09 vs. 3.93 ± 1.09)in the statin treatment groups was were lower than that in control group with statistical significance (P < 0.05). But Whereas there was were no significantly difference between the atorvastatin group and the rosuvastatin group [mALB (14.81 ± 6.23) mg/L vs. (15.31 ± 7.53) mg/L, CysC (1.18 ± 0.25) mg/L vs. (1.15 ± 0.21) mg/L,hs-CRP (3.86 ± 1.15) mg/L vs. (3.93 ± 1.09) mg/L,P﹥0.05). Conclusion Satains Statins may prevent this procedure by anti-inflammatory and anti-oxidative-stress mechanism. Different statins with conventional doses may have the same effect of in the prevention of CIN in patients with normal renal function.

Cervical cancer is the second most common malignant tumor of women all over the world.Infection with oncogenic human papilloma virus ( HPV) types is the most important risk factor to cause cervical cancer.The viral genome of HPV consists of three regions:the early region (E), the late region (L) and the long control region (LCR).The early proteins (E1, E2, E4, E5, E6, E7 ) play vital roles in the continuous virus expression and replication in cells.Among these proteins, E6 protein has the closest relationship with the tumorogenesis and development of cervical cancer.Many mechanisms of the HPV encoded early protein E6 in in-ducing cervical cancer have been characterized, including degradation of p53 protein, activation of telomerase, interaction with PDZ protein, disturbance of cellular signaling pathways, inhibition of immune recognition and blockage of cellular apoptosis.HPV E6 pro-tein may be a preferred target in prevention and treatment of cervical cancer.