Highly active antiretroviral therapy (HAART) can effectively suppress thehuman immunodeficiency virus (HIV) replication andsignificantly reduce morbidity and mortality of HIV-infectedpatients,which howevercan't completely remove the virus,and eventually progressinto chronic viral-infection disease.Chronic HIVinfection destroys host immune system,leading to intestinal barrier damage,intestinal mucosal dysfunction,microbial translocation,and further accelerates the disease progress.The reconstruction of intestinalmicroflora balance and improvement of intestinal mucosa function areessential to reestablish the host immune system.This paper will review the current research advanceson intestinal barrier damage of HIV infection and gut-target therapy of AIDS.The aim is to provide valid evidences for further research targeting improvement of treatment strategiesandreduction of morbidity and mortality in HIV infection.

Modern fully automatic biochemical analyzer makes enzyme measurement accurate, specific and practical, but alarm failure happens sometimes and thus determinate error appears. To find out the inducement to alarm failure, Olympus AU2700 and ADVIA 1650 fully automatic biochemical analyzer are used to measure the serum liver enzyme from 450 cases of post-liver-transplantation patients.

Yaoyangguan ( GV 3 ), Shiqizhui ( Ex-B 8) and Yanglingquan (GB 34) were selected as main points and a three-part needle insertion technique was used to treat 32 cases of lumbar intervertebral disc protrusion. The curative rate was 96.9%. Deep puncture is the key to the treatment.

Objective To explore the expressions of histamine receptor subtypes (H1, H2, H3, H4 receptor) in children's mid-urethral striated muscles and during mouse C2C12 striated myogenesis.Methods Children's mid-urethral striated muscle samples were paraffin embedded and tissue sections were made, then immunohistochemical staining was used to check H1, H2, H3, H4 receptors.C2C12 myogenesis was induced, the early differentiation early markers of desmin, middle marker of myogenin, late marker of myosin heavy chain and histamine 4 receptor subtype mRNA were checked by quantitative real-time polymerase chain reaction.Immunofluorescence staining was done to check 3 differentiation markers and histamine H3 receptor protein.Results During myogenesis, the expression of desmin mRNA in the differentiation of 2,4,6 days were 12,68,60 times as many as that of the undifferentiated myoblasts;the expression of myogenin mRNA in the differentiation of 2,4,6 days were 631,1 408,914 times as many as that of the undifferentiated myoblasts;the expression of myosin heavy chain mRNA in the differentiation of 2,4,6 days were 7 718,9 448,286 288 times as many as that of the undifferentiated myoblasts.The expression level of H1 receptor mRNA in the differentiation of 6 days was about 25％ to undifferentiated cells;the expression of H2 receptor mRNA in undifferentiated cells and differentiated cells groups had no significant difference (F =1.47, P ＞ 0.05);the expression of H3 receptor mRNA in the differentiation of 2,4,6 days was 28,103,198 times to undifferentiated cells;H4 receptor mRNA was not detected.In immunofluorescence staining, H3 receptor protein staining intensity increased with the differentiation.Immunohistochemistry of pediatric urethral striated staining indicated that H1, H2, H3 receptor staining was positive,H1 receptor showed strong positive staining, H3 receptor moderate positive staining,and H2 receptor showed weak positive staining.Conclusions Histamine receptor subtypes of H1 receptor, H2 receptor and H3 receptor were found during mouse striated myogenesis and in the children's mid-urethral striated muscles.The increasing expression of H3R with myogenesis might indicate it plays a role in mature striated muscle cells.

Objective To investigate the number and activity changes of endothelial progenitor cells(EPCs) in hemodialysis patients,and explore its correlation with the risk factors of coronary heart disease.Methords Total mononuclear ceils(MNCs) were isolated from peripheral blood of patients with chronic renal failure in long-term hemodialysis and from a normal control group by Ficoll density gradient centrifugation and then were plated on humanfibronectin-coated dishes.Mter 7 days of culture,EPCs were characterized as adherent cells by double staining with FITC-UEA-I and DI-LDL,and were further identified by the expression of CD34,CD133 and KDR with flow cytometry.EPCs migration was determined with modified Boyden chamber assay.EPCs adhesive assay was performed by replanting EPCs on humanfibronectin-coated plates and then counting the adherent cells.The relationship of the EPCs' number and activity with Framingham Risk Score of ten years was also be assessed.Results Number of EPCs and the migratory & adhesive capacity were significantly lower in patients than in the control(P

This paper aimed to present the surface modification of tissue engineering materials and its correlation with cell compatibility from the aspects of cell-compatibility polymer surface group transformation and bioactive molecule immobilization.

BACKGROUND: The development of tissue engineering has provided a possibility for repairing and reconstructing tissues or organs. However, studies on biomedical tissue-engineered and polymer tissue-engineered materials need to be investigated. OBJECTIVE: To clarify the content of tissue engineering and the application of polymer material in tissue engineering from the point of biocompatibility. RETRIEVAL STRATEGY: Using the terms "tissue engineering, tissue engineering materials, Polymers materials, bio-compatibility, bio-compatibility materials, cell-compatibility, cell-compatibility materials", we retrieved PubMed database to identify studies published between January 1990 and December 2007 in the English language. At the same time, we searched Wanfang database with the same terms in the Chinese language. After primarily selected, 81literatures were kept. Inclusive criteria: studies, whose contents are related to biocompatibility of tissue-engineered materials. Exclusive criteria: repetitive studies or Meta analysis. Thirty literatures corresponded to the inclusive criteria, and fifty-one were rejected due to obsolete or repetitive contents. Among the 30 included literatures, 19 were about biocompatibility, and the remaining 11 about cellular compatibility materials. LITERATURE EVALUATION: The included studies were mainly from Pubmed database and Wanfang database. A total of 25 treatises and 5 reviews were kept. DATA SYNTHESIS: The content of tissue engineering consisted of seeded cell inoculation, biomaterial implanting and cell transplantation. Allogenic, autogenous, and xenogenous tissues were in vitro broken into cells, and then reconstructed through inoculation and proliferation by gene reconstruction technique. Much attention should be focused on how to reconstruct tissue-engineered materials with materials and living cells, I.e. To reconstruct active materials with biological functions. Tissue-engineered materials should have the best interface reaction effect between material surface and cells. Therefore, the core of studying tissue-engineered materials is to design a device, which has chemical molecular level and three-dimensional molecular level cell/material mixed surface, and also has a three-dimensional molecular level appearance corresponding to biomechanical requirement. Polymer materials have good physical mechanical functions, and their molecular structures are closer to living body. Therefore, polymer materials are widely used as biomaterials and exert an important role in the field of tissue engineering. CONCLUSION:To study biomaterials with good tissue compatibility is the basis for tissue engineering development. Polymer materials are widely used in the tissue engineering due to their good property and molecular structure closer to living body.

DNA, Fungal ; isolation & purification ; Humans ; Mass Screening ; Occupational Diseases ; diagnosis ; microbiology ; Polymerase Chain Reaction ; Skin ; microbiology ; Tinea ; diagnosis ; microbiology

Objective:To investigate the mechanism of the changes of the intestinal mucosal barrier in Severe acute pancreatitis.Methods:Twenty patients with Severe acute pancreatitis were admitted and 10 volunteers as the control group.The levels of serum tumor necrosis factor alpha(TNF_?)、nitric oxide(NO)、diamine oxidase(DAO) and the concentration of plasma endotoxin(ET) were measured.The ratio of lactulose to mannitol in urine was detected by HPLC with Pulsed Electrochemical Detection.Results:Compared with the control group,significantly increasing parameters could be seen in all the patients with Severe acute pancreatitis,including the ratio of lactulose to mannitol in urine (P

Objective To observe the effect of comprehensive community intervention on cardiovascular risk factors in metabolic syndrome (MS) high risk group.Methods One hundred MS high risk patients were divided into intervention group (50 patients) and control group (50 patients) by random digits table.The intervention group accepted intervention on health education,health action,health habit and individual intervention on drug.The control group accepted natural intervention.The changes of waistcircumference,body mass index (BMI),blood pressure,fasting plasma glucose (FPG),lipids and serum uric acid (UA) were observed.Results In intervention group,the number of physical exercise,alimentary control and medication compliance increased 12％,16％ and 18％,and the number of smoking and alcohol drinking decreased 8％ and 6％.But in control group,the number of physical exercise,alimentary control,medication compliance increased 2％,0 and 4％,and the number of smoking and alcohol drinking decreased 4％ and 2％.There was significant difference (P＜ 0.05).Before treatment,the level of waist circumference,BMI,systolic pressure,diastolic pressure,FPG,total cholesterol,triacylglycerol,high density lipoprotein cholesterol (HDL-C),low density lipoprotein cholesterol (LDL-C) and UA between two groups had no significant difference (P ＞ 0.05).After 1 year's intervention,except HDL-C,the level of other index had significant difference between two groups (P ＜ 0.05 or ＜ 0.01).Conclusion Comprehensive community intervention can improve the status of obesity and dyslipidemia,and reduce blood pressure,plasma glucose and UA.