Purpose:To study the salvage treatment of recurrent intermediate—high grade non Hodgkin lymphoma(NHL). Methods:24 cases of recurrent intermediate—high NHL were divided into two groups: 10 cases with poor prognostic factors in group one(G1), 14 cases without poor prognostic factors in group two(G2).All cases were treated with ifosfamide(IFO), mitoxantrone(Mx), pingyangmycine(PYM), prednisone(Pred) (IMPP). IFO 1.5 g/m 2 iv on days 1 to 4, and mesna iv at hours;0, 4, 8 Mx 8 mg/m 2 on day 1; PYM 5mg/m 2 im on days 8 and 15; and Pred 100mg/d po on days 1 to 5. Every 4 weeks as one cycle. Assessment was given after 2 cycles. Results:The total CR rate was 58.3%(14/24), RR 79.2%(19/24). The CR rate was 30%(3/10), RR 60%(6/10) in G1 and the CR rate was 78.5% (11/14), RR 92.8%(13/14) in G2 ,respeetively. The difference of the two groups in CR and RR was significant( P

Non-invasive transcutaneous monitoring(TCM) of oxygen and carbon dioxide,with continuous,non-invasive characteristics,is in common use and significant in the neonatal intensive care unit (NICU),which can directly reflect critically ill newborns'changes of respiratory and circulatory function.This review describes how to use TCM appropriately,compares the advantages and disadvantages of TCM with other monitoring devices,introduces its application in neonatal transport and NICU.

Objective: To explore the efficacy and mechanism of warm needling moxibustion plus percutaneous kyphoplasty (PKP) for osteoporotic vertebral compression fracture (OVCF) due to kidney deficiency and blood stasis. Methods: A total of 70 OVCF patients were randomized into a control group and an observation group, with 35 cases in each group. The control group was given PKP treatment, and the observation group was treated with warm needling moxibustion on the basis of the treatment in the control group. The visual analog scale (VAS) and Oswestry disability index (ODI) were scored and the Cobb angle of fractured vertebrae was measured before and after treatment. The re-fracture rate of the adjacent vertebrae was recorded at 1-year follow-up. The serum levels of transforming growth factor (TGF)-β1 and omentin-1 were measured before and after treatment. Results: After treatment, the VAS and ODI scores in both groups decreased (all P<0.05), and all the scores in the observation group were lower than those in the control group (both P<0.05). After treatment, the Cobb angle of fractured vertebrae in both groups decreased (both P<0.05), and the Cobb angle in the observation group was smaller than that in the control group (P<0.05). At 1-year follow-up, the re-fracture rate of the adjacent vertebrae in the observation group was lower than that in the control group (P<0.05). After treatment, the serum levels of TGF-β1 and omentin-1 in both groups increased significantly (all P<0.05), and the serum levels of TGF-β1 and omentin-1 in the observation group were higher than those in the control group (both P<0.05). Conclusion: The treatment of warm needling moxibustion plus PKP can relieve pain, improve dysfunction, promote healing of the injured vertebrae, and reduce the re-fracture rate of the adjacent vertebrae in patients with OVCF due to kidney deficiency and blood stasis, which may be related to the increase of serum TGF-β 1 and omentin-1 levels.

Humans ; Child ; Brain Diseases ; Influenza, Human/diagnosis* ; Influenza A virus

In the scientific,systematic,practical and feasible principles,the quality control needs and management needs of health authorities and medical institutions were analyzed.The study probed into the real-time monitoring functions of existing hospital information systems and information products of IT technology firms,and the feasibility of setting up key monitoring points during medical service processes.685 real-time monitoring indicators have been set up for real-time monitoring of medical process quality.

The article describes the development of new magnetic bead separation and purification instrument. The main application of the instrument is to capture tubercle bacillus from sputum. It is a pretreatment instrument and provides a new platform to help doctors to diagnose bacillary phthisis. Not only could it be used for tubercle bacillus capturing, but also for gene, protein and cell separating and purification. Because the controller of the instrument is 16-bit single chip microcomputer, the cost could be greatly reduced and it will be widely used in China.
Cell Separation ; instrumentation ; Equipment Design ; Magnetics

Objectives To culture and isolate Malassezia furfur which were suspected in sputum smears,and then to identify the isolates systematically.Methods From March 2010 to September 2011,133 lower respiratory tract secretion samples were collected in Beijing Hospital of the Ministry of Health which were suspected containing Malassezia furfur by Parker ink staining.The samples were inoculated and cultured on CHROMagar mediums containing 1％ Tween 60 ( which was modified from Candida chromogenic media) in the atmospheric environment at 35 ℃.The suspected colonies were selected and cultured on both SDA plates containing 0.5％ Tween 40 and SDA plates containing 0.5％ Tween 60.The traditional phenotypic identification methods were used to identify the pure colonies,including staining,growth on Sabouraud agar plates,Tween test,decomposing Escalin,Catalase test,Tween precipitation test and castor oil test.Then the suspected colonies were identified by analyzing the sequences of 18s rRNAs.Results The isolation rate of Malasseziafurfur from lower respiratory tract secretions was 47.4％ (63/133) and 63 strains were also identified as Malassezia furfur by traditional phenotypic identification methods combined with the 18s rRNA gene sequence analysis.Malassezia can be significantly distinguished from Candida by direct smear of the specimen with Parker ink staining.Conclusions Malassezia furfur was found as pink colonies on modified Candida chromogenic media which were significantly different from other Candida colonies.The isolation rate of lower respiratory tract secretions can be improved by modified Candida chromogenic media.The identification accuracy can be further improved by combining traditional phenotypic identification methods with 18s rRNA sequence analysis.

Ischemia-reperfusion injury is common in clinical treatment.Free radical induced oxidative stress plays an important role in this process.Recently,it is reported that hydrogen can selectively scavenging hydroxyl radical in rodents,thus alleviate ischemia-reperfusion injury.Since hydrogen is easy to penetrate the barriers in the body with littie adverse effects,it is hopefully to become a promising agent against ischemia-reperfusion injury in the future.

Objective To investigate neuro-biochemical changes of bilateral anterior cingulate cortex (ACC) in the patients with major depressive disorder (MDD) and the correlation between abnormal metabolism and the cognitive function before and after treatment.Methods Fifteen patients with major depression and 15age-,sex-and education-matched healthy controls (HC) were involved.The neurochemical abnormalities including the levels of N-acetylaspartate (NAA),choline-containing compounds,glutamate/glutamine and myoinostol were measured by single-voxel proton magnetic resonance spectroscopic (1H-MRS).The subjects were then assessed with executive function with neuropsychological tests including Wisconsin cards sorting test (WCST),verbal fluency Task (vF),StrooP Color and Word Test (SCWT).After this,the patients took selectivity serotonin reuptake inhibitors (SSRIs) for eight weeks.Then,we examined the changes in NAA,Cho,Glx and MI in ACC of patients and assessed their executive function with the neuropsychological tests again.Results 1) In ACC,baseline NAA ((7.36 ± 1.67) mmol/L),GLx ((11.68 ± 1.65) mmol/L) and MI levels ((5.28 ± 0.66) mmol/L) were significantly lower in MDD compared to those of HC (NAA (9.27 ± 1.37)mmol/L,Glx (15.20 ± 1.91)mmol/L,MI (7.80 ± 2.73) mmol/L) (P ＜0.01) ; After treatment,the NAA ((9.34 ± 2.45) mmol/L) and Glx ((16.79 ±3.96) mmol/L) increased significantly after treatment compared to those prior to pretreatment (NAA(7.36 ± 1.67)mmol/L,Glx(11.68±1.65)mmol/L,P＜0.05).2) The normal controls exhibited better in Performance of WCST and Performance of completion time of SCWT than our MDD patients (P＜0.05 or P＜0.01).The patients made significant improvements in Performance of WCST and completion time of SCWT(C form) after treatment (P＜0.05 or P＜0.01).3) In post-treatment MDD,the ACC Glx level was significantly positively correlated with the categories completes of WCST (r=0.739,P=0.009) and inversely related with the numbers to complete the first categories of WCST (r=-0.699,P=0.017) and the completion time of SCWT(C form) (r=-0.651,P=0.030) ;elevated MI(r=-0.705,P=0.023) and NAA(r=-0.735,P=0.010) levels in ACC of post-treatment MDD were both negatively correlated with the numbers to complete the first categories of WCST.Conclusion The ACC may be a key region involved in antidepressant treatment in MDD.The increased ACC NAA,Glx and MI in MDD after treatment may be significantly correlated with the improve of executive function.

The da Vinci robotic surgical system assisted colorectal radical resection has been widely applied in many countries and regions.The advantages of the da Vinci robotic surgical system include stable and distinct stereoscopic vision,short learning curve,convenient operation and feasibility of remote manipulation,while its disadvantages are expensive cost,lack of tremor and prolonged operation time.The advantage of da Vinci robotic surgical system is not distinct when compared with traditional laparoscope in radical resection of colorectal cancer.With the accumulation of clinical data and the update of the system,the prospect of da Vinci robotic surgical system assisted colorectal radical resection is promising.