Respiratory tract infection is a common disease in childhood.Virus plays an important role in respiratory disease.With the progresses in molecular technologies in these years,newly discovered viruses have been identified including human metapneumovirus,human coronaviruses,human Bocavirus,polyomaviruses,new enterovirus and rhinovirus strains.These viruses have been identified to cause childhood respiratory infection.This review overviews the newly recognized respiratory viruses and seek to focus on their contribution to infection and co-infection in respiratory tract infection in childhood.

In this paper,a method of solid-phase luminescence immunoassay(LIA)of human alpha-fetoprotein(AFP)is described.The IgG of horse anti-human AFP-IgG was labelled with 6[N-(4-aminobutyl)-N-enthyl]-amino 2,3-dihydrophthalazine 1,4-dione (ABEI).The sensitivity of the method was 6.25ng/ml.The intra and inter coefficientsof variation was 7.9% and 9.8% respectively.The value of AFP of 20 patients assayedby LIA was compared with that assayed by radioimmunoassay(RIA).Although,the datafrom LIA were lower than that assayed by RIA,the results obtained by two methodscorrelated fairly well with a correlation coefficient(r)of 0.95.

Purpose:To compare the response and toxic reaction of 3-weeks’ and weekly taxotere/cisplation treatment in non-small-cell lung cancer. Methods:Group A: Taxotere 75mg/m~2 IV d 1,cisplatin 25mg/m~2 d 1-3 q3-4w; Group B: Taxotere 60 mg IV d1, taxotere 40 mg IV d8,d15, cisplatin 25 mg/m~2 IV d 1,d 8,d 15 q3-4w. The clinical responses were assessed after two cycles. Toxicity was assessed every cycle. Results:There was no CR in the 71 cases . There were 14 PR, 13 SD in group A; there were 16 PR, 14 SD in group B. The overall response rates were 41.2% and 44.4% in group A and B. The rates of grade Ⅲ/Ⅳ neatropenia were 70.6% and 25% in group A and B. The major nonhematologic toxicity was weakness. The rates of weakness were 44.1% and 19.4% in group A and B. Conclusions:The response rates were similar between groups A and B. The occurrence of hematologic toxicity and weakness were lower in weekly treatment.

Blood transfusion in liver surgery still plays an important role.However,blood transfusions may cause immunosuppression recipients and hinder the body' s immune response against the tumor,promotes tumor metastasis and recurrence.How to resolve this contradiction? We must study and research the effects of allogeneic blood transfusion on liver cancer metastasis and recurrence after hepatic resection,and to find strategies to improve the liver cancer prognosis.

Network resources can exactly make up for it. Making good use of the search engine can expand extracurricular professional English learning for students;provide teachers with lesson plan-ning material and learners with the correct pronunciation of the vocabulary. Network digital repository offer students learning platform and PBL teaching in the QQ group can be tried. Electronic dictionary software helps to read the professional literature and E-mail is a convenient means of communication. These help to create language environment and enhance bilingual teaching effect.

Astrocyte elevated gene (AEG) -1 is cloned as a human immunodeficiency virus (HIV) -1-inducible and tumor necrosis factor-alpha (TNF-α)-inducible transcript in primary human fetal astrocytes (PHFA) by a rapid subtraction hybridization approach. AEG-1 has been reported to be up-regulated in various types of human cancers. Multivariate analyses indicat that AEG-1 correlates with the ability of growth, invasion, metastasis, angiogenesis and chemoresistance of tumors. AEG-1 over-expression activates the PI3K-Akt,nuclear factor kappa B (NF-κB) and Wnt-β-catenin signaling pathways in several crucial aspects of tumorprogression. AEG-1 represents a viable potential target for the therapy of human cancers.

Objective To investigate whether polymorphisms of apolipopretein D gene (APOD) have an effect on the risk for sporadic Alzheimer's disease (SAD).Methods Combination of polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) and DNA sequencing technique to screen all exons (1-5),along with flanking exon-intron boundaries of the ApoD gene.We investigated the polymorphisms of ApoD in 256 SAD patients and 294 healthy controls from North China by PCR-RFLP technique.Association of every polymorphism with AD was analyzed in this case-control study.Results Two ApoD (rs5952 and rs1568566) polymorphisms were detected and there were significant differences in the genotype or allele frequencies for the 2 ApoD polymorphisms respectively between cases and controls.Logistic analysis showed that rs5952C or rs1568566T allele carrier increased the risk for SAD (rs5952 adjusted OR=1.817,95% CI 1.237--2.669,χ2=9.282,P=0.002 ; rs1568566 adjusted OR=1.563,95% CI 1.060-2.306,χ2=5.072,P=0.024).The APOD polymorphisms showed gender specific associations.The linkage disequilibrium of the 2 single nucleotide polymorphism loci was found between rs5952 and rs1568566 of ApoD.Conclusion Polymorphisms of rs5952 and rs1568566 in APOD might play an important role in modifying risk for SAD.

Digital technology is a hot spot in the orthopedic research in recent years.Computer-aided orthopaedic surgery is a kind of interdisciplinary and frontier technology,usually involving preoperative design and plan,virtual operation,intraoperative navigation and implementation.The application of computer-aided techniques in the treatment of proximal humeral fractures was summarized in this article.We intended to inform surgeons of the digital techniques which have been efficiently used in the clinic and improved the efficacy of PHF treatment.

Objective Quantitative evaluation was conducted to assess the development level and trend,as well as discipline distribution of basic medical research in China,based on SCI publications.Methods Descriptive analysis and trend analysis by 5-year moving time window were applied to analyze the quantity,quality,discipline distribution and current status and trend of development of medical research papers published by Chinese scientists during 2004 and 2013;Results Among a total of 280,200 medical research papers published from 2004 to 2013 period,China ranked # 5 in the world in terms of the quantity of papers.However,the average citation of 8.34 for Chinese paper was far below the global average of 14.11.Among all papers published by Chinese scientists,the percentages of papers in the field of psychiatry/psychology,neuroscience/behavior,immunology and clinical medicine were 2.20％,7.59％,3.93％ and 40.67％,respectively,lower than those of global average (6.71％,9.41％,4.62％ and 47.45％).The percentages of papers in other disciplines were higher for China than for global average.Regarding to the development trend,the annual increase in the number of publications of 5 years was 22.24 ％,while the 5-year citation per paper was only 2.03 ％.Conclusions The quantity of basic medical research in China is large and growing rapidly,while the quality of these researches remains in relatively lower level,and need to be improved substantially.With the discipline distribution different from the global average,psychiatry/psychology,neuroscience/behavior,immunology and clinical medicine should be strengthened in the future.