1.Brow position and shape in young Chinese women
Li ZHUANG ; Bo CHEN ; Haihuan MA
Chinese Journal of Medical Aesthetics and Cosmetology 2014;20(2):85-87
Objective To evaluate the the brow position and shape in young Chinese women using a photography measurement.Methods A total of 100 Chinese women aged 20 to 30 years were photographed to determine the distance between brow and eye.Measurements were made from a horizontal plane through the lateral canthus of right eye to three vertical points on the upper brow margin at the medial canthus,lateral limbus of the iris,and lateral canthus.Results The distance between brow and eye in young Chinese women was 2.12,2.52 and 2.46 cm at medial canthus,lateral limbus of the iris and lateral canthus,respectively.The distances were higher between the brows and eyes at lateral limbus of the iris and lateral canthus than that at medial canthus (P<0.05).Conclusions The measurement used in this study is a simple and practical quantitative method.The results can be used to guide the surgical choice and help to evaluate the surgical techniques.
2.Effects of salvia miltiorrhizae on L-Ca current in ventricular myocyte of guinea pig during normoxia, acute hypoxia and reoxygenation
Bo CHEN ; Jihua MA ; Peihua ZHANG
Chinese Journal of Pathophysiology 1986;0(01):-
AIM: To observe the changes of Ca-L current (ICa-L) and to investigate the mechanism of salvia miltiorrhizae (SM) for eliminating Ca~2+ overloaded in cells during acute hypoxia/reoxygenation. METHODS: The whole cell patch clamp technique was applied to study the changes of ICa-L. Different concentrations (32, 320, ~3 200 mg/L) of SM were added to the ventricular myocytes isolated from guinea pigs by enzyme digestion. RESULTS: SM (32, 320, ~3 200 mg/L) decreased the amplitude of ICa-L in a concentration-dependent manner regardless of these cells were under normoxia, hypoxia or reoxygenation. Furthermore, SM at low concentration (32 mg/L) was more effective to hypoxia or reoxygenation-treated cells than that to the cells under normoxia condition. CONCLUSION: These results indicate that SM effectively decreases the abnormal raised amplitude of ICa-L in ventricular myocytes under hypoxia or reoxygenation conditions, preventing Ca~2+ overloaded in the cells. [
3.High-value medical consumables informatized management based on two-dimensional barcode
Chaoqiong MA ; Gang FENG ; Bo CHEN
Chinese Medical Equipment Journal 2015;(9):135-137
The present situation and the deficiencies of high-value medical consumables were analyzed. Two-dimensional barcode technology was suggested to be involved in the whole-course dynamic tracing of the high-value consumables to combine the high-value consumables information with the patient information. The course included the links of acquisi-tion, warehousing, barcode preparation, delivering and charging. Two-dimensional barcode technology may enhance the medical consumables management and decrease medical dispute, and thus contributes to the development of digital hospi-tal.
4.Study on STR Genotyping of Cell Free DNA in Plasma
Yang CHEN ; Liping HU ; Bo MA ; Liyu MA ; Shengjie NIE
Journal of Kunming Medical University 2014;(1):140-143
Objective The purpose of this study was to investigate the feasibility of short tandem repeat(STR) genotyping of cell free DNA in plasma for individual identification and paternity testing. Methods EDTA-Na2 DNA anti-coagulant blood samples were collected from 36 unrelated healthy volunteers,and both DNA in leukocytes and cell free DNA in plasma were extracted respectively using phenol-chloroform method. Target DNA in blood cells and plasma were amplified using regular STR typing and fluorescent multiplex STR assay separately,accordingly,the PCR products were analyzed by polyacrylamide gel electrophoresis and capillary electrophoresis. Results Using either normal PCR-STR or fluorescent multiplex STR assay,the consistent STR genotyping results were detected with similar efficiency for cell DNA and plasma DNA samples from the same individual. Conclusion Cell free DNA in plasma samples can be used as useful biological samples for STR genotyping,which can be applied to individual identification and paternity testing in forensic practice.
6.Correction of minor breast ptosis by subfascia breast augmentation with periareolar incision and anatomic mammary implant.
Jie CAI ; Bo CHEN ; Yang ZHOU ; Haihuan MA
Chinese Journal of Plastic Surgery 2014;30(3):175-178
OBJECTIVETo correct the minor breast ptosis with minimal breast scar.
METHODS32 cases with minor breast ptosis were corrected by subfascia breast augmentation with periareolar incision and anatomic mammary implant.
RESULTBreast ptosis was completely or mostly corrected in all the patitents with periareola scar, avoiding the vertical breast scar. Except for short-time effusion in 2 cases, no other complication happened.
CONCLUSIONSSubfascia breast augmentation with periareolar incision and anatomic mammary implant can effectively correct minor breast ptosis with minimal breast scar and less complication.
Adult ; Breast Implantation ; methods ; Cicatrix ; Female ; Follow-Up Studies ; Humans ; Middle Aged ; Nipples ; surgery
7.Clinical applications of dual plane breast augmentation under endoscope through transaxillary incision
Jie CAI ; Yang ZHOU ; Bo CHEN ; Haihuan MA
Chinese Journal of Medical Aesthetics and Cosmetology 2014;20(2):91-94
Objective To introduce the technical key points and clinical efficacy of dual plane breast augmentation under endoscope through a transaxillary incision.Methods By analyzing the 89 surgical cases,we have summarized the key points of the pre-operative design,surgical skills and postoperative management.Results All patients had no uncontrolled bleeding during operation and loss of nipple and areola sensation.71 patients got 6-12 months follow-up,showing that only one case presented with unilateral capsular contraction,and the others had satisfied results.Conclusions Blunt dissection can reduce the risks of uncontrolled bleeding on the inner side of the pocket and the damage of the nerves on the lateral side of the dissecting pocket.The lower inner part,lower part and lower lateral part are the areas that could be sharply dissected by electrocautery under endoscope to achieve the dual plane Ⅰ.The drainage and bandage are necessary after the operation.
8.Chest imaging characteristics of hand-foot-mouth disease in children
Bo MA ; Ruigang CHEN ; Shewei DOU ; Xiaonian ZHU ; Dapeng SHI
Chinese Journal of Radiology 2010;44(9):943-945
Objective To study radiological characteristics of hand-foot-mouth disease(HFMD) in children. Methods The chest X-ray films of 1295 children patients of HFMD were analyzed,for the general X-ray manifestations and the evolution. Results A total of 1427 films was obtained from all patients, in which 1203 cases were normal and 224 cases were abnormal. The interstitial changes characterized the abnormal group, mainly as increased and vague lung markings, increased hilar shadows (137 cases).The parenchyma changes appeared as patchy exudative shadows(49 cases). Short-term dynamic observation was applied in 62 cases, 38 cases pulmonary disease progression manifested as normal and the interstitial type changing into the parenchyma type and the mixed type, the localized type changing into the diffuse type. Conclusions Most children patients of HFMD showed normal chest films, while the abnormal patients were characterized by interstitial and parenchyma pulmonary edema. Serial chest X-ray examination and short-term dynamic observation were important to identify the severe cases and assess patients' condition.
9.Case Report and Literature Review of Hypoparathyroidism-Deafness-Renal Dysplasia Syndrome in Children
nai-jun, WAN ; xiao-bo, CHEN ; xin, MA
Journal of Applied Clinical Pediatrics 2004;0(08):-
Objective To explore the diagnosis and treatment of hypopara thyroidism-deafness-renal dysplasia(HDR) syndrome in children.Methods According to the symptoms,signs,laboratory examination and the previous published papers of other authors,the disease of the child was diagnosed and treated,and some related literatures were reviewed.Results The 12-years-old boy presented with tetany and deafness and Chvostek's and Trousseau's signs were positive.The initial laboratory studies showed that the serum concentration of calcium was lower,parathormone was in the low limit of normal range and alkalin phosphatase was normal.An audiography revealed the pattern of sensorineural deafness in all frequencies.Abdominal CT revealed that his right kidney was not observed and creatinine clearance rate was low.After treated with calcium carbonate and vitamin D,the symptoms and the signs were improved.Conclusion HDR syndrome is a rare disorder and less recognized in children,and therefore more attention should be paid to avoid missing diagnosis.
10.Purification and N-terminal Amino Acid Sequencing of the ESM Protease Isolated from an Eggshell Mem-brane-degrading Bacteria
Bo LI ; Yong DANG ; Yu MA ; Ying-Yi CHEN ;
Microbiology 2008;0(08):-
A strain producing eggshell membrane protease (ESM protease) was isolated from the soil and identified as Pseudomonas aeruginosa. The enzyme isolated from the fermentation liquid of this strain and purified by ammonium sulfate precipitation, quadratic anion-exchange chromatography exhibited eggshell membrane degrading activity of 304.5 U/mg. By SDS-PAGE, the protein molecular mass is 32 kD. The N-terminal amino acid sequence of this protease is: Ala, Glu, Ala, Gly, Gly, Val, Ala, Gly, Lys, Glu, Asp, Ala, Ala, Glu, Leu.