[Objective]To investigate the protective effects of rhEPO on neuromotor function and apoptosis of neural cell,after it was peritoneally injected at different time in rats with traumatic spinal cord injury(SCI).[Method]A total of 48 adult male Sprague-Dawley rats weighting 270 ?10 g were randomly divided into four groups.Rats in normal group(n=6)and sham group(n=6)underwent laminectomy producure only.In control group,rats(n=18)received normal saline epidurally at 1,6 and 24 hours after injury.Treatment group(n=18)received 5000 iu/kg body weight of recombinant humane erythropoietin administered epidurally at 1,6 and 24 hours after injury.SCI was induced with 70g/cm impact according to the improved Allen method.Behavioral evaluation of the rats was made 48 hours after trauma using the Basso,Beattie,and Bresnahan(BBB)scoring system and Rivlin's tiltboard experiment.Injured spinal cord tissue cell apoptosis was examined by the terminal deoxynucleotidal transferase-mediated dUTP-biotin nick end abeling(TUNEL)reaction at 48 hour.Fasl and Caspase-3 expression was determined by immunohistochemical analysis at 48 hours after injury.The results were observed by light microscope and analyzed by SPSS statistics software.[Result]Compared to the control group,neuromotor function was significantly improved at 1,6,and 24 hours after injury in the experiment group.The indexes of neural cell decreased significantly(P

Atherosclerosis ; complications ; pathology ; Humans ; Thrombosis ; complications ; pathology

Budd-Chiari Syndrome ; diagnosis ; diagnostic imaging ; Humans ; Magnetic Resonance Angiography ; Magnetic Resonance Imaging ; Tomography, X-Ray Computed ; Ultrasonography

This paper is prepared to discuss the common issues in data management, such as building and training of data management team, standard operation procedure, document management, execution and communication, strategies to correct and prevent mistakes, and measures to improve the quality and efficiency of clinical trials and data management.
Clinical Trials as Topic ; Data Collection ; standards ; Information Storage and Retrieval ; standards

Objective To investigate effect of guanosine diphosphate (GDP) on the mitochondrial respiratory oxygen consumption and the mitochondrial membrane potential (MMP) of rat brain and explore the relationship of the change of uncoupling proteins (UCPS) activity with the oxygen consumption and MMP. Methods The mitochondria of rat brain were isolated by centrifugation. Mitochondria oxidative respiratory consumption was measured by Clark electrode after the treatment of GDP at different concentrations so as to calculate mitochondrial state 3 respiration (ST3), mitochondrial state 4 respiration (ST4), respiratory control rate (RCR), and the rate of oxidative phosphorylation (OPR). MMP was detected by Rhodamine 123 method at the different concentrations of GDP. Results With the increase of GDP concentration form 0 to 1.0 mmol/L, the values of ST3, ST4 and OPR were reduced while RCR was elevated. But when the concentration increased to 1.4 mmol/L, the former 3 indexes begun to increase while the later declined. When the GDP concentration reached to 1 mmol/L, the inhibitory rate was only 35.1%, 51.3%, 14.2% to ST3, ST4 and OPR respectively, while RCR was increased to 133.2%. No matter the concentration was over 1 mmol/L or under 1 mmol/L, the ability of inhibition was attenuated. MMP reached to the highest point when GDP exerted the highest inhibitory rate on mitochondrial respiratory oxygen consumption. Conclusion GDP, an inhibitor of UCPS, can regulate the respiratory oxygen consumption and MMP of the isolated rat brain mitochondrial directly in a dose-effect fashion. The change of UCPS activity can affect the respiratory oxygen consumption and MMP.

Objective To discuss the issue of the limb salvage versus the amputation of serious lower limb injury. Methods The history, the age, the clinical presentation, the treatment time and method of the 72 severe open fractures of the lower limb were studied. Following the patients admission, they were examined completely in emergency room and were sent to radiological department for taking X ray films. According to Gustilo and Andserson classification, there were 48 limbs of type ⅢB and 24 limbs of type ⅢC.16 limbs had multiple trauma as well as severe lower extremity injury. Among them, there were 7 (9.7%) limbs with head or spine injury, 5(6.9%) limbs had abdominal trauma, 4(5.6%)limbs had thoracic trauma. Results 16 limbs in 72 limbs were amputated, in which 7 limbs were type ⅢB and 9 limbs were ⅢC. 4 limbs in 8 limbs of more than 50 years old were amputated. The ratio of amputation was 50.0 %. 12 limbs in 64 limbs younger than 50 years old were amputated. The ratio of amputation was 18.8%.Their comparison has statistically significance(?2=4.018, P

Objective To explore serratus anterior muscle transplantation for the repair of soft tissue defects in foot and ankle. Methods Seventeen patients consisted of 11 males and 6 females aging from 13 to 58 years with the mean age of 35.2 years. The anatomical parameters of serratus anterior of 17 cases were measured, the thickness of the muscle was (1.24?0.65) cm, and the diameter of origin point of the nutrient artery to the muscle was (1.4?0.6) mm. There were two veins arising from the muscle, the diameter of larger one was (2.5?0.8) mm. The length of vascular pedicle was (8.31?1.48) cm. 17 patients with soft tissue defect of foot and ankle were treated with free serratus anterior muscle transfer and split skin graft overlying the muscle. The primary lesions were soft tissue defect around the ankle and Pilon fracture in 5 cases, non-union of distal tibial fracture in 3, Achilles tendon and soft tissue defect in 3, metatarsal fracture and soft tissue defect in 4, and calcaneal fracture with soft tissue defect in 2. All of the patients suffered from infection or exposure of bone or applied plate, the area of soft tissue defect ranged from 4.5 cm?6 cm to 11 cm?13 cm. Results The average operative time was (6.5?1.2) hours. The complications included local hematoma in 2 cases, superficial infection in 2, winged scapula without function loss in 1,scar pain in 2, and numbness of lateral thoracic wall in 1. Furthermore, weakness and decreased shoulder mobility were noted in 3 patients comparing with the contralateral side, but the condition improved half year following the operation. The transferred muscle flap appeared thinner and less bulky , and healed intimately with the adjacent foot tissue, the patients resumed good function in walking. Conclusion The serratus anterior muscle flap is a good choice for treatment of soft tissue defects in foot and ankle.

Objective The activity and cyclooxygenase (COX-2) protein expression of colorectal adenoc arcinoma cell treated by deoxycholic acids (DCA) were examined in order to find out the carcinogenesis mechanism of DCA. Methods The proliferation of colorectal cancer cells (SW 1116) was detected by MTT metho d . COX-2 protein expression was measured by immunohistochemistry and Western blo t. Results DCA lower than 100 ?mol/L concentration can stimulate the growth of the adenoca rcinoma cells with effect reversible, while higher concentration shows the long -acting effect of inhibition. DCA of 10,50 and 100 ?mol/L concentration can up -regulate the expression of COX-2 in cells, while only 10 ?mol/L can maintain this effect long than 72 hours. The level of COX-2 protein treated by the late r two concentration descends after 48 hours. Conclusion DCA affects the proliferation of SW1116 in two sides. D CA concentration lower than 100 ?mol/L can promote COX-2 protein expression, w hich may be the mechanism of its carcinogenesis.

Objective To investigate the effects of serotonin(5-HT) on the ionic channels currents and membrane potentials of single guinea pig proximate colon myocytes. Methods Cell images before and after contraction were captured and analyzed with an imag e analysis software. Channels currents, action potential (AP) and resting potent ial (RP) were recorded with an EPC-9 amplifier. Results The direct actions of 10 ?mol/L 5-HT to myocytes resulted in followin g effects: Firstly, the exogenous stimulated peak values of AP reduced to 60%?7 .3%(n=7,P

Objective To study the one-month and one-year effect of 2006' Fujian International "Quit&Win" Competition,and to explore the influencing factors for smoking-quitting behaviors.Methods A equidistant sampling survey was conducted among the 4619 participants at the end of one-month and one-year after the competition by telephone,mailing and face to face interview.The survey contents include personal information,smoking-quitting and relapse information.The data were input with EPIDA3.1 software,?2 test and logistic stepwise regression were done with SPSS 11.5.Result The rate of smoking-quitting sustained for one-month was 51.9%,and the rate of smoking-quitting sustained for one-year was 38.0%.With one-way Chi-square test,the influencial factor on smoking-quitting succession for one-month was their intention of joining in the competition(?2=75.799,P=0.000);and the influencing factors on smoking-quitting succession for one-year were cigarette consumption per day(?2=9.953,P=0.019),their intention of joining in the competition(?2=52.227,P=0.000) and the method of smoking-quitting(?2=7.882,P=0.019).With logistic regression analysis,the influencial factors on smoking-quitting succession for one-month were age,their intention of joining in the competition and the method of smoking-quitting,the OR values were 0.98(0.964～0.997),1.198(1.195～3.080) and 2.812(2.152～3.675),respectively;the influencial factors on smoking-quitting succession for one-year were marriage,cigarette consumption per day,their intention of joining in the competition and the method of smoking-quitting,the OR values were 1.915(1.085～3.381),0.720(0.546～0.951),0.529(0.330～0.847),3.171(2.233～4.501) and 1.901(1.294～2.793),respectively.The major factors for relapse were the surrounding people smoking,lack of support,et al.Conclusion The 2006' Fujian International "Quit & Win" Competition gained a great success and has important significance for smoking-control work.