Objective To investigate the difference of the glial cell line-derived neurotrophic factor and its receptor content of proximal neurons after nerve grafting was used to reconstruct C5 root in young rats.Methods Model of C5 resection was set up in 12 18-day-old SD rats.Experimental animals were divided in to two groups, one group for C5 resection, another for nerve grafting to reconstruct the C5 defection.At 4 weeks postoperatively, the immunohistochemical staining was performed and the number of GDNF and GFRa1 immunohistochemical positive neurons were calculated respectively.Results The number of GDNF positive neurons in spinal cord and dorsal root ganglion of C5 repairing group was 786.3 ± 176.84 and 2997.0 ±357.99, and that of C5 resection group was 335.0 ± 49.50 and 1632.0 ± 305.55.On the other hand, the number of GFRa1 positive neurons in spinal cord and dorsal root ganglion of C5 repairing group was 787.5 ±178.55 and 3111.0 ± 445.72, that of the other group was 397.3 ± 41.78 and 1588.3 ± 229.00.The statistical analysis result showed GDNF and GFR immunohistochemical positive neurons in spinal cord and dorsal root ganglion of C5 repairing group was statistically more than that of C5 resection group(P < 0.01 ).Conclusion The neuronal protective effect of nerve grafting after reconstructing brachial plexus nerve injury in young rats may be attributed to the increase of GDNF and its receptor GFRa1 content of proximal neuron.

Objective To investigate the effect of nerve grafting to enzyme histochemical changes on neurons after brachinal plexus nerve in jury in young rats. Methods Model of C5 resection was set up in 24 18-day-old SD rats. Experimental animals were divided in to two groups, one group for C5 resection, another for nerve grafting in repairing the C5 defection. At 4 weeks postoperatively, cholinesterase (CHE) and acidphosphatase (ACP) histochemical stain of neurons in C5 anterior horn and dorsal root ganglia (DRG) were detected. Results Compared with C5 resection group, bio-activity of CHE of C5 repairing group was statistically higher, and that of ACP was statistically lower. Conclusion Nerve grafting has protective effect on survival of neurons after brachial plexus nerve injury in young rats.

Objective To disclose the relationship of the target muscle function and different time interval after nerve grafting reconstructed C5 root resection in young rats.Methods Model of C5 resection was set up in 48 18-day-old SD rats.The rats were randomly divided into C5 resection group,immediate repairing group,3 days delayed repairing group,and 6,9,12,15,18 days delayed repairing groups.Each group experienced nerve grafting bridged the C5 nerve root defection at its time interval.At 6 weeks postoperatively,electrophysiological and histochemical experiment were performed.Results There was no statistical difference among the data of CMAP amplitude and latency and weight of target muscles and number of distal myelinated fiber of immediate repairing group and those of 3,6 days delayed repair group at 6 weeks postoperatively,but compared with C5 resection group,the dates was statistically higher.There was no statistical difference between the data of C5 resection group and that of 15,18 days delayed repairing group.Conclusion Nerve reconstruction for C5 root injury in young rats within 0-6 days (equal to 0-4 months in human beings) has a satisfactory protective effect on target muscles.It suggests that the OBPP children who have the operation indication should undergo surgical management in 4 months after their birth.

Objective To disclose the relationship of neuronal protective effect and different time interval after nerve grafting reconstructed C5 root resection in young rats.Methods Model of C5 resection was set up in 18-day-old SD rats from Jauary 2009 to December 2009.Forty-eight rats with C5 resection were randomly divided into C5 resection group,immediate repairing group,three days delayed repairing group,and 6,9,12,15,18 days delayed repairing groups.Each group experienced nerve grafting bridged the C5 nerve root defection at its time interval.At 4 weeks postoperatively,the numbers of True Blue positively labeled neurons in all groups were counted respectively.Results There was no statistical difference among the number of proxinal neuron of immediate repairing group and those of 3,6 days delayed repair group (P ＞ 0.05),but compared with C5 resection group,the number of neurons was statistically higher (P ＜ 0.05).There was no statistical difference between the number of motoneurons of immediate repairing group and that of 9 days delayed repairing group(P ＞ 0.05),but there was statistical difference between sensory neurons of this two groups(P ＜ 0.05).The neuron number of inmediate repairing group was statistically higher than those of 12,15,18 days delayed repairing group(P ＜ 0.05).Conclusion Nerve reconstruction for C5 root injury in young rats within 0-9days (equal to 0-6 months in human beings) has a satisfactory protective effect on proximal neuron.It suggests that the OBPP children who have the operation indication should undergo surgical management in 6 months after their birth.

This review summarizes the research related to early rehabilitation after cardiac surgery, Including the methods, programs, evaluation and effects of rehabilitation.It is proposed that individualized early rehabilitation after cardiac surgery should be provided; the effect of early rehabilitation needs to be further explored; future research should focus on the medium and long-term effects of rehabilitation.

ZJ-XD-I multifunctional internal sterilization machine for medical instrumentation is applied in medical instrumentations with circulatory loop such as anesthetic machine and respirator. The machine can generate ozone and hydrogen peroxide, and then the two gases are mixed and introduced into the internal loop to eliminate adnexed hydrogen peroxide such as the virus and germ, and thus the nosocomial infection due to repeated uses of medical instrumentations. The machine is composed of the gas compression component, atomization unit, gas extraction component, control circuit and the filtrating unit.

Objective: To evaluate the potential dose influence to organs at risk (OARs) and targets of adaptive radiotherapy (ART) for non-small cell lung cancer (NSCLC). Methods:Twice positional CT images of 12 patients with locally advanced NSCLC were captured during radio-(n=3) or radio-chemotherapy (n=9) for ART simulation. The twice positional scanningplan was fused using MIM software. The variation of irradiation doses for the lung, heart, and spinal cord was evaluated, and the prescription doses for the targets were escalated. Results:Adaptive radiation enabled dose reduction by an average of 3.53%for lung V20 and by 2.55%for V30. The mean dose for the lung decreased by 2.11 Gy. The dose was reduced by an average of 4.17%for heart V30 and by 3.37%for V40. Meanwhile, the maximum dose for the spinal cord was reduced by 3.52 Gy on average. Lung sparing with ART enabled an iso-mean lung dose escalation of the Planning gross tumor target volume dose, which improved by an average of 1.25 Gy. Conclusion:The adap-tation of radiotherapy for continuous tumor shrinkage during the treatment course for NSCLC reduces doses to OARs, enables signifi-cant dose escalation, and has the potential to increase local control.

To investigate the mechanism of electroacupuncturein treating chronic fatigue syndrome(CFS) in term of the neuro-endocrine system by observing the regulative effect of EA on hypothalamic-pituitary-adrenal index (HPA index) and corticotropin releasing hormone mRNA (CRH mRNA) in CFS model rats. Methods: The rats were randomly divided into control group, model group, and electroacupuncture group. Model and electroacupuncture groups were forced to swim in cold water to make CFS model, while rats in electroacupuncture group were treated with electroacupuncture treatment at the same time. Electroacupuncture group was given at Baihui(GV 20) and Zusanli(ST 36). Evaluation of the model was processed according to the behavior changes of the rats. Hypothalamic, pituitary, and adrenal were weighted to calculate HPA index. CRH mRNA in hypothalamic was measured by fluorescence quantitative polymerase chain reaction (FQPCR). Results: The model rats had behavior changes,and both physical and mental fatigue was observed. HPA index raised and expression of CRH mRNA in hypothalamus increased in CFS rats. After electroacupuncture treatment, the physical and mental fatigue was improved, and the hypothalamic index and CRH mRNA decreased significantly, while the pituitary and adrenal index only had little decrease. Conclusion: Cold water swim press could mimic the pathogenesis and make similar manifestations in rats with the common clinical symptoms. CFS has close relationship with increase of HPA index and CRH mRNA expression. Electroacupuncture can regulate the function of HPA axis to deal with CFS.

0.05).The optimal combination was PCR-CTPP for MDR1 C3435T and PCR-SSP for G2677T/A.Conclusions PCR-CTPP and PCR-SSP are simple,accurate,rapid and economical methods for detection of SNP of MDR1 C3435T and G2677T/A,and can be applied in clinical research.

OBJECTIVE To study the molecular cytogenetic alterations of transitional cell carcinoma of the urinary bladder with exfoliated cells by fluorescence in situ hybridization(FISH) analysis of chromosome-specific probes.METHODS FISH was performed using 3,7,9 and 17 of chromosome-specific probes to examine chromosome aberration of exfoliated cells in 50 urine samples from patients with transitional cell urinary bladder carcinoma.RESULTS The frequency of numerical aberration of chromosomes 3,7,9 and 17 was 28%,32%,56% and 38% in urinary exfoliated cells,respectively.Loss of chromosome 9 was the most common finding,but it was not correlated with pathological grade of cancer and stage of the disease.Abnormality of chromosomes 3,7 and 17 was associated with the clinical stage.CONCLUSIONS A number of chromosome aberrations are detected in transitional cell carcinoma of the urinary bladder by FISH technique which provides a basis for further understanding of its molecular pathogenesis.It is a rapid,accurate and very sensitive method and can be used in clinical diagnosis.