Objective To observe the effects of aerosol imiquimod on the level of Interleukin-12 (IL-12) and interleukin-13 (IL-13) in bronchial alveolar lavage fluid(BALF) and serum immunoglobulin E(IgE) level in mouse asthma models.Methods Thirty BALB/c mice were divided into 3 groups, control group, asthma group and imiquimod group, ten in each group.The asthma group and imiquimod group were established by sensitization and challenge with ovalbumin (OVA) ,imiquimod group was interfered with aerosol imiquimod 30 min before challenge.Serum in each group was collected by excising eyeball 24 h after the last challenge.The level of IgE in serum was measured by ELISA.Bronchoalveolar lavage fluid (BALF) was performed, and total cell counts,cell differential were examined.The level of IL-12 and IL-13 in BALF was measured by ELISA and total cell counts, cell differential were examined in BALF.The inflammation changes of lung was observed by HE staining.Results (1) By hematoxylin-eosin (HE) staining, the result of asthma mice were small airway wall thickening ,secretion increased, epithelial cells fall off, arranging disorderly, and submucosal had plenty of inflammatory cells infiltration, mainly with lymphocytes.But imquimod group attenuated the airway inflammation of asthmatic mice.(2) Total cell counts and all kinds of inflammatory cells of asthma group were increased greatly compared with normal group;Eosinophil and lymphocyte cells were much lower in imquimod group than those in asthma group (P ＜0.05).(3) The level of IL-13 in BALF and the level of IgE in serum in imiquimod group was obviously higher than that in control group,IL-13 level were positively correlated with IgE level(r =0.774 ,P ＜ 0.05), but imiquimod group was greatly decreased compared with control group and increased in comparison with that in group A;The level of IL-12 in asthma group was reduced and the level of IgE was increased compared with normal group.IL-12 level in imiquimod group was evidently higher and IgE level was lower than that in asthma group.IL-12 level negatively correlated with IgE level(r =-0.802, P ＜ 0.05).Conclusion Imiquimod aerosol inhalation may up-regulate the level of Th1 chemokine IL-12,down-regulates the level of Th2 chemokine IL-13 and decrease the level of IgE in serum so that it may suppress antigen-induced airway inflammation.

Objective To investigate the inhibition of Sophora flavescens extract on the degree of inflammatory swelling of guinea pigs'skin with eczema, observe pathological changes and explore the effects of the anti-in-flammatory action, in order to provide experimental basis for clinical treatment.Methods Eczema models of 40 guinea pigs were set up by smearing 2, 4-dinitrochlorobenzene on their napes to cause sensitization and motivating in the inside of their right ears.All guinea pigs were randomly divided into 5 groups equally,including the model com-pared group, positive control group and high, medium, low concentration groups of sophora flavescens.After eczema skin was continuously administered drugs for 14 days, the same part with equal area of the left and right ears was taken and weighed.The difference between the left and right ears was used to evaluate the swelling degree of skin inflammation.The pathological changes were observed by making pathological slices with HE staining with the tissue blocks of right ears.Results Compared with the model group, the swelling degree of skin inflammation was significantly relieved and dermal edema and inflammatory cell infiltrating were significantly improved in the high and medi-um concentration groups of sophora flavescens (p<0.05).Compared with the positive group, the swelling degree of skin inflammation, dermal edema and inflammatory cell infiltrating were significantly alleviated in the high concentra-tion group of sophora flavescens ( p<0.05) .Conclusion Sophora flavescens extract can effectively reduce inflam-matory response of guinea pigs'skin with eczema, having a certain significance for the clinical treatment of eczema.

Objective To optimize the extraction technology of Morchella polysaccharide.Methods With GIM5.69(Morchella esculenta(L.) Pers.) and GIM5.29(Morchella elata) as the research object,the difference of polysaccharide yield with lixiviation in polysaccharide extraction between ultrasound and enzyme was observed.At the same time,the difference of polysaccharides yield of these two Morchella was compared.Result Ultrasound and enzyme both can improve the polysaccharides yield,and the effect of 10 min of ultrasound was the best.The polysaccharides yield of GIM5.69 was higher than that of GIM5.29,which means different strain has different polysaccharides yield.Conclusion The best extraction condition is 10 min of ultrasound at the frequency of 40 kHz,heating 150 min at 90 ℃ and lixiviation twice.

Objective To investigate the effects of USP4 on the proliferation of human hypertrophic scar fibroblasts(HSFB).Methods HSFB were cultured in vitro.The fourth generation of HSFB in logarithmic growth phase was selected in the experiment.HSFB were intervened by Vialinin A which was the inhibitor of USP4 for 0,12,24,48 h,then collected cells and the their expression of USP4 and TβRI and Smad7 protein was detected by Western blot.MTT assay was used to detect the effect of Vialinin A on the proliferation of HS fibroblasts and the cells were divided into experimental group and control group(without intervention).Results After treatment with Vialinin A in HSFB,the expression of USP4 and TβRI protein in HSFB decreased gradually,especially in 12 h(P<0.05),and Smad7 protein expression was increased gradually(P<0.05).The proliferative activity of intervened HSFB reduced gradually,the difference between experimental group and control group was statistically significant(P<0.05).Conclusion USP4 might inhibit the proliferation of scar cells and down-regulation of USP4 expression in hyperplastic scar fibroblasts,which can slow proliferative activity of intervened HSFB by regulating TGF-β/Smad signaling pathway.

It showed that physical therapy is important to the old people with type 2 diabetes mellitus by reviewing literature. The mechanism maybe lose body weight, raise glucose utilization, and reduce complication. Physical therapy is useful to decline the level of blood glucose, relieve complication, and improve quality of life.

Adult ; Ear Canal ; pathology ; Ear Neoplasms ; Female ; Humans ; Neurofibromatoses

Child ; Erythropoietin ; adverse effects ; Humans ; Male ; Recombinant Proteins ; Red-Cell Aplasia, Pure ; chemically induced

Objective To describe the recommended nursing undergraduate students' real feelings during participating in the selection test of comprehensive quality,so as to provide reference for improving the quality of nursing undergraduates education.Methods 12 recommended nursing undergraduate students were surveyed by using deep half-structural interviews and were recorded,noted during the whole interviews.Data were analyzed by coding step by step.Results The primary feelings of the participants included:Reflection on theory gap of imbalanced knowledge structure,competence of English language affected by periodical study and lack of real situation,and comprehensive quality training was weak after classes.Conclusions Nursing humanistic education,English learning styles and comprehensive quality training should be taken seriously and implement deep reform in nursing higher education.

To explore the relationship between serum total bile acid concentration and fetal prognosis in patients with intrahepatic cholestasis of pregnancy (ICP).A total of 131 ICP patients were divided into mild and severe ICP groups according to serum total bile acid concentration.And another 100 normal subjects during the same period were selected as control group.Their medical records and fetal prognoses were analyzed.The serum total bile acid,liver enzyme and lipid concentration of ICP patients were all significantly higher than those of the controls (P ＜ 0.01).The fetal prognoses of ICP patients were significantly different with those of controls (P ＜ 0.05).A high serum level of total bile acid of ICP patients may produce serious adverse effects on fetal prognosis.

Objective To study the differences in serum antioxidant ion status of adults living in different regions and relat-ed influential factors.Methods Serum levels of total antioxidation capacity(TAOC),superoxide dismutase(SOD)and malon-dialdehyde(MDA)were determined in37local residents in Xinjiang(Xinjiang group)and37Tianjin residents(Tianjin group).Results Serum levels of TAOC and SOD were significantly higher in the Xinjiang group than that in the Tianjin group(P0.05)for the MDA between the two groups.The TAOC levels were sig-nificantly higher in the females than that in the males(P