Objective To establish HPLC fingerprint for the identification of Semen Cassiae. MethodsChromatographic fingerprint of Semen Cassiae was investigated by RP-HPLC and the gradient elution mode was applied to chromatographic separation. Data were analysed by fingerprint similarity evaluation software to compare the similarity of the samples. ResultsThe HPLC fingerprint of Semen Cassiae was established preliminarily. ConclusionHPLC Fingerprint method is repeatable and can be used in quality control of Semen Cassiae.

Facial Neoplasms ; surgery ; Head and Neck Neoplasms ; surgery ; Humans ; Jaw Neoplasms ; surgery ; Mouth Neoplasms ; surgery ; Oral Medicine ; Otolaryngology ; Specialties, Surgical

Objective To evaluate the effects of controlling arterial oxygen partial pressure (PaO 2) at the beginning and during cardiopulmonary bypass (CPB) on cyanotic myocardial protection. Methods 40 Children suffering from tetralogy of Fallot(TOF) under 3 years of age with SpO 2

6, 39 cases were with 3～6, 13 cases were with the number less than 3. The diameter of stones was 0.5～1.2 cm. Five cases suffered from bile leakage, 2 cases from peritonitis and 2 cases from remnant stones in the common bile duct. Forty-one patients were followed up and doing well. Conclusions Surgical approach is choosen on the basis individually. Laparoscopic common bile duct exploration for gallstone concomittant with choledocholithiasis is safe.

Objective To evaluate the effect of biofeedback therapy for the treatment of myogenic fecal incontinence. MethodsA general assessment about anorectal function was made on 17 cases receiving biofeedback therapy including muscle power training,sensory training and coordination training. Results The clinical scores before and after biofeedback therapy were 1.66?0.23,3.80?0.42 respectively,with an effective rate of 82%. The anus maximum contracting pressure elevated,(73?20) mm Hg vs. (123?30) mm Hg; myoelectric amplitude increased,(122?32) ?V vs. (230?41) ?V;Contracting time prolonged,(4.1?2.0) s vs. (9.4?3.0) s; The sensory threshold was lowered,(50?12) ml vs. ( 20? 10) ml;The feel-contract time increased,(3.1?0.4) s vs. (1.2?0.3) s. Positive rectal contraction reflex was seen in 41% patients before therapy compared with 82% after therapy. Conclusions Biofeedback therapy increases contractility of sphincter,decreases threshold of rectal sensory,and is a therapy of choice for myogenic fecal incontinence.

Objective To prepare the anti-hUART1 polyclonal antibody and investigate the subcellular localization of hURAT1 protein. Methods The full-length hURAT1 gene was obtained by RT-PCR and inserted into the fusion expression vector pEGFP-N3,the predicated antigen epitope was cloned into GST fusion protein expression plasmid pGEX-5X-1, transformed into E. coli BL21 cells for expressing recombinant GST-hURAT1 protein induced by IPTG. The purified hURAT1-GST fusion protein was employed to immunize rabbit for preparing the polyclonal antibody. The expression of hURAT1 was analyzed by western-blot and immunohistochemistry in human kidney. pEGFP-hURAT1 was transfected into the LLC-PK1 cell in order to observe the subcellular localization of the gene using confocal microscopy. Results Specific anti-hURAT1 rabbit polyclonal antibody was obtained, and both Western-blot and immunohistochemistry showed that hURAT1 was expressed in the human kidney brush border,localized in the apical membrane of the LLC-PK1 cell. Conclusions hURAT1 protein was a membrane protein located in renal proximal tubule, which could be detected in the apical membrane. The anti-hURAT1 polyclonal antibody could be used for studying the physiological function of hURAT1 and its pathology.

Objective To review our experience of surgical treatment for postinfarction ventricular septal defect. Methods From 1990 to 2004, 22 patients with postinfarction ventricular septal defect underwent surgical treatment. There were 15 males and 7 females with a mean age of (62.2?7.3) years (from 52 years to 72 years),19 had anterior ventricular septal defect and 3 had posterior VSD, and there were 18 cases with left ventricular aneurysm.10 patients also had introaortic balloon pumping in the early experience. In all the patients cardiac function were in NYHA class III～IV. Results There were 8 operative deaths (35%). There were 7 operative deaths before 1999 and 1 case after 1999. The other patients are clinically asymptomatic after surgery, and the cardiac function is in NYHA class I～II. Conclusion Postinfarction ventricular septal defect is an infrequent but serious complication of myocardial infarction. Surgery is the best way to treat this condition. A coronary angiography should be performed in all patients who can be stabilized hemodynamically and myocardial revascularization should be performed in patients with significant coronary artery stenosis.

Congenital Abnormalities ; diagnosis ; Ear, Inner ; abnormalities ; Humans ; Vestibular Evoked Myogenic Potentials

Objective To know the blood level and the influencing factors for the children living in the area near highway in the mountain region. Methods From December,2006 to April,2007,one hundred and eighteen children aged 8-12 years (exposed group) living in the area near highway in the mountain region and fifty-one children aged 8-12 years (control group) living in the area far away from the highway were selected by random cluster sampling. Lead level of the soil and crops on the highway-sides (pollution area)and the area far away from the highway (control area) was determined. The basic situation was investigated by the questionnaire, the blood lead level was determined by graphite furnace atomic absorption spectrometry. The influence of lead on children's intelligence, physical growth and the influencing factors for blood lead level were analyzed. Results The average lead level of the soil [(96.89?15.05) mg/kg] and crops [(0.86?0.29) mg/kg] in the polluted area was significantly higher compared with the control [soil lead: (29.83?1.41)mg/kg,crops lead:(0.17?0.03) mg/kg], and revealed statistical significance respectively (P