Objective To investigate the quality of marriage and sexual function of cervical cancer patients following radical hysterectomy and vaginal extension.Methods Case-control and questionnairebased methods were used in this study.Forty patients of early-stage (Ⅰ b 1 ～ Ⅰ b2) cervical cancer who had undergone vaginal extension following classic radical hysterectomy were included in the observation group,while 40 patients with matching factors and radical hysterectomy only during the same period were included in the control group.The quality of marriage and sexual function were compared with OLSON marriage prolapse and incontinence sexual function questionnaire Shon Fonn (PISQ-12).Results The operation time and postoperative vaginal length in the observation group were higher than those in the control group (P ＜ 0.05 or P ＜ 0.01).The emotional,physical,and total scores during postoperation were lower than those during preoperation (P ＜ 0.01).The emotional,physical,and total scores in the control group were lower than those in the observation group (P ＜ 0.01).The marital satisfaction,husband and wife communication,conflict resolution methods,extracurricular activities and sex scores in the observation group were higher than those in the control group (P ＜ 0.05 or P ＜ 0.01).Conclusions Patients with peritoneovaginoplasty following radical hysterectomy had much longer vagina and less self-perceived short vagina.Vaginal extension following radical hysterectomy does not worsen the pelvic floor symptoms.

OBJECTIVE:To optimize the extraction conditions of volatile oil from Chrysanthemum indicum and the processing techniques of its ?-yclodextrin(?-CD) inclusion compound.METHODS:The extraction conditions of volatile oil from Chrysanthemum indicum was optimized taking soaking time,ultrasonic treatment time,water amount as influencing factors and with the yield of the volatile oil as index.The processing techniques of the ?-yclodextrin (?-CD) inclusion compound was optimized taking the ratio of ?-CD to volatile oil,the time and temperature of the inclusion process as indexes and with the yield of the inclusion compound and the inclusion rate of the volatile oil as indexes. RESULTS:The optimal process for oil extraction was as follows:the soaking time=1.5 h,the ultrasonic treatment time= 40 min,heating time=6 h,water amount=600 mL.The optimal processing conditions for(?-CD) inclusion compound of Chrysanthemum indicum were as follows: the ratio of ?-CD to volatile oil=8∶1,the inclusion time of the compound was 2 hours at 40 ℃.CONCLUSION:High extraction yield of vlatile oil and stable inclusion compound were obtained from the above method.

Most neonatal diabetes mellitus (NDM) is caused by genetic abnormality. Sulfonylurea (SU) has been successfully applied in NDM patients;The pathogenesis of NDM and mechanism of sulfonylurea on molecular level have been illuminated, and recent studies revealed that glycated albumin is a useful glycemic indicator. In this review, the research progress of NDM is summarized.

Objective To investigate the effect of transfusion of allogeneic apoptotic splenocytes on immunological tolerance to pancreatic islets transplantation in rat. Method Recipient SD rats were rendered diabetic mellitus by a single intraperitoneal injection of streptozotocin (STZ). The apoptosis of donor Wistar rat splenocytes was induced by X-irradiation with electron linear accelerator. Pancreatic islets were isolated by intraductal collagenase P, and were purified by Ficoll-400 discontinuous density gradient after culturing for 1-2 days from donor Wistar rat. The diabetic SD rats were randomly divided into 4 groups, and respectively received infusion of Hanks solution, donor normal splenocytes, donor apoptotic splenocytes, and donor necrotic splenocytes via dorsal veins of penis, and then they received pancreatic islets from the same donor rat underneath their renal subcapsular space 7 days later. Mixed lymphocyte reaction (MLR) was used as immunological parameter of transplantation tolerance besides the observation of blood glucose level and allograft survival time. Islet graft failure was defined as the presence of a plasma glucose concentration of at least 11.0mmol/L on 2 successive days. Results The pre-infusion of donor apoptotic splenocytes significantly prolonged the survival time of allograft as shown by the median survival time (MST) of graft of 31days, and the longest survival time of 42 day, and it apparently decreased the rejection response in recipient SD rat in one-way mixed lymphocyte culture. Conclusion Transfusion of donor apoptotic splenocytes could induce immunological tolerance of allogenic islets transplantation in rat.

Objective To determine the planning target volume margins of head and neck cancers treated by image guided radiotherapy (IGRT).Methods 464 sets cone beam computed tomography (CBCT) images before setup correction and 126 sets CBCT images after correction were obtained from 51 head and neck cancer patients treated by IGRT in our department.The systematic and random errors were evaluated by either online or offline correction through registering the CBCT images to the planning CT.The data was divided into 3 groups according to the online correction times.Results The isocenter shift were 0.37 mm±2.37 mm, -0.43 mm±2.30 mm and 0.47 mm±2.65 mm in right-left (RL), anterior-posterior (AP) and superior-inferior (SI) directions respectively before correction, and it reduced to 0.08 mm±0.68 mm, -0.03 mm±0.74 mm and 0.03 mm±0.80 mm when evaluated by 126 sets corrected CBCT images.The planning target volume (PTV) margin from clinical target volume (CTV) before correction were:6.41 mm,6.15 mm and 7.10 mm based on two parameter model, and it reduced to 1.78 mm,1.80 mm and 1.97 mm after correction.The PTV margins were 3.8 mm,3.8 mm,4.0 mm;4.0 mm,4.0 mm,5.0 mm and 5.4 mm,5.2 mm,6.1 mm in RL, AP and SI respectively when online-correction times were more than 15 times, 11-15 times,5-10 times.Conclusions CBCT-based on online correction reduce the PTV margin for head and neck cancers treated by IGRT and ensure more precise dose delivery and less normal tissue complications.

Objective To investigate the prevalence rate of hypertension ,overweight ,obesity and obesity/overweight ,thus to explore the relationship between hypertension and sleeping time .Methods Healthy individuals having physical examinations in Health Management Center of Southwest Hospital were randomly selected to take questionnaire and physical examination ,and then we analyzed the correction relationship of hypertension and sleeping time .Results The prevalence of overweigh ,obesity ,hyperten‐sion and perhypertension was 36 .68% ,11 .36% ,22 .14% and 33 .43% respectively .The prevalence rate of hypertension for men was 2 fold as that of women .There was a negative correlation between hypertension and sleeping time .The prevalence of stage 2 hypertension tended to U.People who sleep time less than 5 h have more risk for Isolated systolic hypertension than who sleeping 6 h .Conclusion There is a high risk of hypertension and stage 2 and stage 3 hypertention for people whose sleep time less than 5 h or more than 10 h .

BACKGROUND:The large watercress molded piece is longer than that of the normal molded piece, which is suitable for dental restoration of elderly patients with gingival recession and relatively long crown.
OBJECTIVE:To test and verify the effect of large watercress molded piece on the auxiliary repair of approximal caries lesions in molars of the elderly.
METHODS:Eighty-seven senile patients with molar approximal caries were randomly divided into two groups: those were repaired by large watercress molded piece as experimental group, and those repaired by traditional amalgam molded piece ascontrol group. Then we compared the comfortable satisfaction rate, the excelent and good rate of repair and the gingival index between the two groups immediately, 1, 3, 6 and 12 months after repair.
RESULTS AND CONCLUSION:The comfortable satisfaction rate of the experimental group was significantly higher than that of the control group at 1, 3, 6 and 12 months after repair (P< 0.05), and the gingival index was significantly lower than that of the control group (P< 0.05). The excelent and good rate of the experimental group was significantly higher than that of the control group at different time points after repair (P< 0.05). No loss or fracture of the restorations occurred in the two groups. These results demonstrate that the large watercress molded piece for repairing approximal caries lesions in molars of the elderly can better restore the anatomical shape and approximal points of teeth, and reduce food impaction and gingival inflammation.

Objective To investigate the expression of aquaporin-4 (AQP4) in contused brain and contralateral side and correlation of AQP4 level with brain edema.Methods A total of 70 health adult SD rats were divided into sham operation group (n =10) and brain contusion group (n =60) according to the random number table.Except for no impact in sham operation group,the remaining operations were the same in both groups.Ten rats in brain contusion group were separately sacrificed at 1,6,24,48 and 72 hours,and 7 days.Pathological changes in brain tissues of rats were detected and immunohistochemistry staining and Western-blot were used to measure AQP-4 expression.Results There were no abnormalities of brain tissue structure in sham operation group with AQP4 expressed mainly in glial cells and vascular endothelial cells.With respect to the injured side in brain contusion group,mixed brain edema that consisted of angioedema and cellular edema occurred along with tissue necrosis,massive inflammatory cell infiltration,and microgial proliferation.AQP4 was apparently expressed in gliocytes and vascular endothelial cells,which showed a fall at 1 hour,the lowest at 6 hours,the peak at 24 hours,slow fall at 48 hours,a second peak at 72 hours,and then a fall until 7 days.Level of AQP4 other than that at 1 hour had significant difference compared with sham operation group (P ＜ 0.01).Whereas the contralateral side in brain contusion group showed no pathological abnormalities at 1 hour,but cellular edema emerged at 6 hours followed by mixed brain edema mainly characterized as cellular edema at 24 hours,continued cellular edema but alleviated angioedema at 48 and 72 hours,and alleviated cellular edema at day 7.AQP4 was up-regulated in gliocytes and vascular endothelial cells,which decreased at 1 hour (1.313 ± 0.01),minimized at 6 hours (0.922 ± 0.03),peaked at 24 hours (2.848 ± 0.020),fell at 48 hours,peaked again at 72 hours (2.662 ± 0.02) and fell to almost normal level at day 7.By contrast with sham operation group,level of AQP4 had significant difference other than that at 1 hour and 7 days (P ＜ 0.01).Conclusions After brain contusion,angioedema and subsequent cellular edema emerge in the contused side.Pathological changes are delayed in non-contused side with cellular edema ahead of angioedema.Level of AQP4 is closely related with traumatic brain edema.