1.One case report and analysis of patient with renal immunoglobulin light and heavy chain deposited disease
Guang YIN ; Yan WU ; Caihong ZENG ; Huiping CHEN ; Zhihong LIU
Clinical Medicine of China 2014;30(10):1063-1066
Objective To report 1 case with renal immunoglobulin light and heavy chain deposited disease (IgG-κ light chain and γ1 heavy chain).Methods The clinical manifestations,serum immunofixation electrophoresis,light and heavy chain abnormalities of blood and urine,bone marrow biopsy and renal biopsy data laboratory data were recorded and analyzed.Results A 63-year-old woman presented with massive proteinuria,microscopic hematuria,hypertension,anemia and serum IgG-κ light chain.Bone marrow aspirate revealed 4% plasma cells.Renal biopsy revealed nodular glomerulopathy with congo red staining-negative.Immunofluorescence showed that κ light chain and IgG1 (γ1 heavy chain) were deposited along the glomerular basement membranes (GBM) and tubular basement membranes (TBM).Electron-microscopy revealed electrondense deposits that delineate the outer aspect of TBM and endothelial aspect of GBM.Conclusion The diagnosis of renal immunoglobulin light and heavy chain deposited disease (IgG-κ light chain and γ1 heavy chain) should be addressed combine with clinical and is pathology,especially with immune pathological examination.
2.The expressions of aquaporins in chronic stress depression model rat hippocampus and gastrointestinal mucosa
Weixi SONG ; Tingting CHEN ; Guang ZENG ; Yanping TANG ; Beiyang CHEN ; Guomin ZHANG
Chinese Journal of Behavioral Medicine and Brain Science 2013;22(9):794-796
Objective To study the mechanisms of depression by exploring expressional differences of AQPs in the tissues of chronic stress depression rats.Methods Depression model was replicated by unpredicted chronic stress.20 rats were randomly separated into normal control group and model control group,AQP4 and AQP3,AQP8 expressions on hippocampus and gastrointestinal mucosa of rat model with depression were detected by immunochemical staining method.Results Means of optical density of AQP4 of normal group and model group hippocampus were 0.28 ± 0.02,0.22 ± 0.06 respectively,and the difference between two groups was significant statistically(t value was 2.756,P<0.05).The expression of AQP3 on gastric mucosa and colonic mucosa between two groups had no significant statistically(t value were 1.814,1.812,P>0.05).Two groups'means of optical density of AQP3 on small intestinal mucosa were 0.15 ± 0.02,0.17 ± 0.02,and the difference was significant statistically (t value was 2.769,P<0.05).Two groups'means of AQP8 optical density in gastric mucosa were 0.15± 0.01,0.19 ± 0.04 ;0.16 ± 0.01 and 0.21 ± 0.04 in small intestinal mucosa;0.16 ± 0.01 and 0.22 ± 0.04 in colonic mucosa,and the differences were significant statistically(t values were 3.139,5.113,4.534,P<0.05,P<0.01,P<0.01).Conclusion The expression of AQP4 on depression model rat hippocampus are lower than those of the normal group ; and the expression of AQP3 on gastric mucosa and colonic mucosa are no change obviously,but it(')s up-regulation on small intestinal mucosa.The expressions of AQP8 on gastric mucosa and small intestinal mucosa and colonic mucosa are up-regulating.
3.Design of a wireless ambulatory blood pressure monitoring system based on ARM.
Jian-guo ZENG ; Guang-meng CHEN ; Jian WANG
Chinese Journal of Medical Instrumentation 2006;30(6):424-427
A design of the wireless ambulatory blood pressure monitoring system has been presented based on ARM embedded microprocessor and GPRS module for the extention of native mobile telemedicine. The whole system framework and flow chart have been introduced in detail. Based on RBF neural networks, the ambulatory blood pressure data curve has been simulated, and the trend curve provides a reliable gist for the prevention, treatment, diagnosis and therapeutic efficacy of cerebrocardiac diseases.
Algorithms
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Blood Pressure Monitoring, Ambulatory
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instrumentation
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Computer Communication Networks
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Equipment Design
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Humans
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Neural Networks (Computer)
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Signal Processing, Computer-Assisted
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instrumentation
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Software
4.Changes of C-type natriuretic peptide and neurotensin in rabbits brain injury induced by endotoxin.
Yu-cai ZHANG ; Ding-hua TANG ; Xue-guang ZHANG ; Liang XU ; Li-qin CHEN ; Jihua ZENG
Chinese Journal of Pediatrics 2003;41(2):144-145
Animals
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Brain Injuries
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blood
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cerebrospinal fluid
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chemically induced
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Endotoxins
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toxicity
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Female
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Male
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Natriuretic Peptide, C-Type
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analysis
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Neurotensin
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analysis
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Rabbits
5.Effects of chemokine CCL18 on the proliferation and invasion of a human melanoma cell line A375
Chongmei XIN ; Guang LI ; Jianfang SUN ; Yiqun JIANG ; Hao CHEN ; Xuesi ZENG ; Xiulian XU
Chinese Journal of Dermatology 2010;43(12):833-836
Objective To investigate the effects of chemokine CCL18 on the proliferation and invasion of a human melanoma cell line A375. Methods Human peripheral blood monocytes were isolated from healthy volunteers, cultured in vitro and divided into two groups to be induced by IL-4 for 48 hours or remain untreated. A375 cells were classified into 3 groups to be cultured with IL-4-induced monocytes, untreated monocytes or CCL18 of 200 g/L for various durations. A375 cells receiving no treatment served as the control.MTT assay was performed to detect the proliferation of A375 cells, chemotaxis test and Matrigel-transwell assay to evaluate the chemotaxis and invasion ability of A375 cells, and chicken chorioalllantoic memebrane (CAM)was used to detect the effect of CCL18 on tumor angiogenesis. Results The proliferation of A375 cells was statistically accelerated by IL-4-induced monocytes and untreated monocytes, but unaffected by CCL18.Matrigel-transwell assay revealed that IL-4-induced monocytes and CCL18 promoted the chemotaxis and invasion ability of A375 cells (all P<0.05). Tumor angiogenesis was also increased by IL-4-induced monocytes and CCL18 (both P < 0.05). Conclusion The chemokine CCL18 can promote the invasion ability of A375 cells and tumor angiogenesis.
6.Role of intervention in combination with choledochoscopic in treatment of biliary obstruction after liver transplantation
Lin WEI ; Wentao JIANG ; Zhijun ZHU ; Guang CHEN ; Zhigui ZENG ; Zhongyang SHEN
Chinese Journal of Hepatobiliary Surgery 2010;16(5):363-365
Objective To evaluate intervention in combination with choledochoscopic in treatment of biliary obstruction after orthotopic liver transplantation.Methods A retrospective review was performed in the 36 patiants with biliary strictures and bilary cast after the liver tiamsplantalion.All the patients received changing catheters,T-tube sinus tract dilation,choledochoscopic treatment,keeping catheters drainage and supporting.Result 26 patiants got satisfactory results and 8 controlled.The left 3 patients underwent retransplantation.Conclusion Intervention in combination with choledochoscopic treatment is effective to deal with biliary obstruction after liver transplantation.
7.Cloning and Expression of Vibrio cholerae CTB Gene and the Recombinant CTB Protein Activation Assay
Guo-Guang ZHANG ; Ya-Ming ZENG ; Dong-Xiao LI ; Hong-Xin ZHANG ; Liang CHEN ;
China Biotechnology 2006;0(10):-
CTB protein possessed mucosal adjuvant immunoactivity. The CTB gene was amplified by PCR method from a strain V. cholerae. The nucleotide sequence of CTB gene was 375 bp and shared 96.0%~99.2% homology with other 6 CTB genes. The recombinant plasmid pTWIN1-CTB transformed E. coli strain BL21(DE3) expressed with 0.8 mmol/L IPTG. The molecular weight of expression products was identical with expectative weight by SDS-PAGE electrophoresis. The CTB fusion proteins mainly assembled inclusion bodies and the outputs of proteins were approximately 20% of the total bacterial proteins. The CTB proteins possessed mucosal immunoactivity by GM1-ELISA assay.
8.Pharmacokinetics and relative bioavailability of tramadol hydrochloride tabletin Chinese healthy volunteers
Bo CHEN ; Zhao-Hong ZENG ; Guo-Guang MAO ; Yue-Ming MA ; Qiu ZHONG ;
Chinese Journal of Clinical Pharmacology and Therapeutics 1999;0(04):-
0. 05). Conclu-sion The THT and THC have bioequivalence.
9.Preventive effects and mechanism of heme oxygenase-1 activation on acute respiratory distress syndrome in rats.
Jing CHEN ; Jin-yuan ZHAO ; Guang-zeng WANG
Chinese Journal of Industrial Hygiene and Occupational Diseases 2005;23(3):199-202
OBJECTIVETo explore the possible mechanism of heme oxygenase-1 (HO-1) induction for its protective effects on acute respiratory distress syndrome (ARDS).
METHODSThe production of HO-1 was induced by hemoglobin (Hb) injection into oleic acid (OA) induced ARDS rats. Western blot and RT-PCR techniques were used to observe the induction of HO-1 in vivo. Both the levels of tumor necrosis factor-alpha (TNF-alpha) and interleukin-8 (IL-8) in serum and bronchoalveolar lavage fluid (BALF) were measured by enzyme linked immunosorbent assay (ELISA). Combined with blood gas analysis and other indexes, the effect of HO-1 on ARDS could be clearly manifested.
RESULTSThe expression of HO-1 mRNA was increased significantly 16 h after Hb injection, and the protein expression of HO-1 was also obviously increased 24 h later (P < 0.01). The levels of TNF-alpha and IL-8 in both serum and BALF were significantly lower in Hb + OA group than in OA group (P < 0.01). Arterial blood PaO(2), oxygen saturation, and oxygenated index in Hb + OA group [(56.28 +/- 6.71) mm Hg, (79.53 +/- 5.82)%, and (258.81 +/- 29.37) mm Hg respectively] were higher than in OA group [(35.08 +/- 4.59) mm Hg, (55.80 +/- 12.76)%, and (167.86 +/- 21.94) mm Hg]. Lung wet and dry weight, and pathological changes were also improved.
CONCLUSIONThe production of HO-1 was successfully induced by hemoglobin in vivo. Protective effects of HO-1 on ARDS might be related to the decreasing in inflammatory factors, such as TNF-alpha and IL-8.
Animals ; Blotting, Western ; Bronchoalveolar Lavage Fluid ; chemistry ; Enzyme-Linked Immunosorbent Assay ; Heme Oxygenase-1 ; biosynthesis ; genetics ; Hemoglobins ; pharmacology ; Interleukin-8 ; metabolism ; Male ; Oleic Acid ; RNA, Messenger ; genetics ; Rats ; Rats, Sprague-Dawley ; Respiratory Distress Syndrome, Adult ; metabolism ; prevention & control ; Reverse Transcriptase Polymerase Chain Reaction ; Tumor Necrosis Factor-alpha ; metabolism
10.Study on the establishment of data management system of emergency response in fighting against flooding in China.
Wei CHEN ; Li-Jia BAI ; Guang ZENG
Chinese Journal of Epidemiology 2004;25(12):1028-1031
OBJECTIVETo make the emergency response to the diseases control and prevention with flooding more scientific ally-based and efficient.
METHODSHubei and Jiangxi province were chosen as the study fields and to establish a data management system of emergency response on flooding using remote sensing (RS) and geographical information system (GIS).
RESULTSSpatial and basic data together with data on flooding were linked to the code of location, and established on the platform of GIS. Through this system, information on flooding was able to be more rapidly and visually analysed.
CONCLUSIONUsing this system, the extent of flooding and the possible epidemic of some communicable diseases during flooding could be ascertained and predicted scientifically and the resource and capacity of emergency response to the disease control and prevention in flooding areas could also be evaluated.
China ; Databases, Factual ; Disaster Planning ; organization & administration ; Disasters ; Environmental Monitoring ; Female ; Geographic Information Systems ; organization & administration ; Humans ; Male ; Management Information Systems