Objective To investigate the efficacy of flupentixol and melitracen combined with pinaverium bromide in the treatment of patients with irritable bowel syndrome. Methods A total of 64 patients with irritable bowel syndrome from October 2014 to October 2015 in department of gastroenterology of Zhuji People’s Hospital were collected and randomly divided into two groups, with 32 cases in each group.Patients in control group received with pinaverium bromide by oral, patients in combination group received flupentixol and melitracen by oral on the basis of control group.The gastrointestinal symptom rating scale (GSRS), self-rating anxiety scale (SAS), self-rating depression scale (SDS) score and clinical efficacy pre-and post-treatment were compared between two groups.Results Compared with pre-treatment, the main symptoms score in two groups decreased significantly (P<0.05); compared with control group, the main symptoms score in combination group decreased significantly(P <0.05).Compared with pre-treatment, the SAS and SDS scores decreased significantly(P <0.05); compared with control group, the SAS and SDS scores in combination group decreased significantly ( P <0.05 ) .There was no significant difference in total efficacy between control group and combination group ( 93.75%vs.81.25%, χ2 =1.237,P=0.266).There was no adverse event in two groups during treatment.Conclusion Flupentixol and melitracen combined with pinaverium bromide could significantly improve the anxiety and depression in the treatment of irritable bowel syndrome, which could promote the recovery of gastrointestinal function.

Objective To elaborate the progress on prevention and treatment of Chinese medicine to delayed diarrhea induced by Irinotecan. [Methods] In recent 5 years, traditional Chinese medicine treatment of the disease literature was reviewed . [Results] Traditional Chinese Medicine in prevention and treatment of this disease has unique advantages.[Conclusion] Traditional Chinese Medicine in prevention and treatment of this disease should be unified on syndrome type and evaluation criteria,enhance the efficacy, accepted by the majority of patients.

Objective To explore the extraction and assaying of the alkaloids from Bozhou Peony Paeonia lactiflora. Methods Dry root powder of the medical material was extracted by 90%ethanol. The extraction was first acidified by hydrochloric acid after recovering alcohol, and then extracted by dichloromethane. The acid solution was adjusted pH to alkalescence by ammonia water, then extracted by chloroform to get crude alkaloid. Bozhou Peony alkaloids were confirmed by TLC along with different colouration method, and its content was detected by acid-base titration. Results Four alkaloid-representative salmon patches were observed by colored with improving potassium heptaiodobismuthate method, and two prunosus blotches, four dark prunosus spots were delected by concentrated sulfuric acid and formaldehyde-concentrated sulfuric acid method, respectively. However, no chromogenic spots were found by colored with nitric acid as well with ninhydrin. The content of alkaloids in Bozhou Peony Paeonia lactiflora was about 0.079%. Conclusion Bozhou Peony Paeonia lactiflora contained alkaloid, and may including aconitum alkaloids.

Objective To analyse and solve the difficulties and countermeasures in continuously proving medical gas. Methods Aiming at the difficulties of continuously proving medical gas, oxygen produced are mostly canned by oxygen compressor, and are transported to every function module through connecting oxygen jars. Results Medical gas module was taken into full play to meet reasonable demand for continuously providing oxygen. Conclusion It is suitable for other field module hospital to use for reference in the action of medical succor of disaster.

ObjectiveTo review the experience with surgical removal of meningiomas in the falx cerebri and sagittal sinuses.MethodsThirteen patients with meningiomas in the falx cerebri and sagittal sinuses were retrospectively analyzed.ResultsTumors in eleven patients were totally removed( Simpson Grade Ⅰ and Ⅱ ),while in two patients they were subtotally resected( Simpson Grade Ⅲ and Ⅳ ).After operations,no patient died.ConclusionAdequate surgical exposure,efficient management of operative bleeding,protection and replacement of the sagittalsinus,and avoidence of damage to the brain tissues were the key factors to improve operative effect.

Objective To evaluate the effect of antler polypeptides from Cervus elaphus yarkandensis on MC3T3-E1 cells. Methods The concentration of antler polypeptides of Cervus elaphus yarkandensis was measured by BCA protein assay kit. MC3T3-E1 cells were cultured and analyzed by BCIP/NBT chromogenic alkaline phosphatase (ALP) staining kit. After being induced to form mineralized knot, the cells were stained by using Alizarin red staining. Three different concentrations (10, 1.0, 0.1 μg/mL) of antler polypeptides were analyzed by MTT method and micronutrients enzymes standard method to determine the effect of cell proliferation and ALP synthesis. Results The concentration of antler polypeptides was 0.07 mg/mL. The results of in vitro cell activity analysis showed that the positive rate of ALP was 90%and the mineralization knot was stained red. Compared with the control group, the different concentrations of antler polypeptides all showed the function of cell proliferation and the effect was dose-dependent after 3 d and 7 d. Compared with the control group, at the 3 d, three groups of antler polypeptides promoted synthesis and secretion of ALP (P<0.01) and the results showed a dose-dependent effect. Conclusion Antler polypeptides could obviously promote the proliferation of MC3T3-E1 cells and secretion of ALP, which indicated that antler polypeptides have certain effect on osteoporosis.

Objective To explore the efficacy and safety of conversion from calcineurin inhibitor (CNI) to sirolimus (SRL) as major immunosuppressive therapy in lung transplant recipients.Method Retrospective analyses were conducted for the clinical data of all the patients undergoing lung transplantation in Wuxi People's Hospital between January 2011 and December 2014.Sixteen were given conversion treatment with Sirolimus in the postoperative irnmunosuppressive therapy.We analyzed the opportunity and reasons in the conversion treatment,and the safety,effectiveness and complications of the conversion treatment.Result The follow-up period was 8 to 25 months,and the median time of conversion was 6 months after operation (2-18 months).The indications of conversion concluded:malignant tumor (n =8),renal dysfunction (n =5),lymphangioleiomyomatosis (n =1) and intractable diarrhea caused by CNIs (n =2).Four cases suffered from interstitial pneumonitis associated with Sirolimus and one case suffered from spontaneous pneumothorax,and they all conversed back to CNIs.In those patients,cancer recurrence occurred in 4 cases (of them,there were 3 deaths),and 3 patients developed chronic rejection.Those recipients receiving the conversion treatment due to renal dysfunction showed recovery of renal function to some extent.Conclusion It's effective and safe to converse the immunosuppressive therapy based on Sirolimus.Sirolimus should be reduced or withdrawn when interstitial pneumonitis associated with Sirolimus occurred.

[Abstract ] Objective The tension-free vaginal tape is the main method for treatment of stress urinary incontinence (SUI). The aim of the present study was to analyze the effect and safety of tension-free vaginal tape ( TVT) and tension-free vaginal tape-obtu-rater ( TVT-O) for female stress urinary incontinence and to sum up the experiences . Methods Clinical data of 142 patients who re-ceived anti-incontinence surgery ( TVT/TVT-O ) between January 2011 and May 2014 were retrospectively analyzed , 111 cases re-ceived TVT and 31 cases received TVT-O.The effect and the complication were analyzed at least 6 months post surgery .Incontinence quality of life ( I-QOL) and organ prolapse/urinary incontinence sexual function questionnaire ( P-ISQ) were used to observe the sub-jective symptoms .One hour pad test value was used to assesse the objective response . Results The effective rate post-TVT/TVT-O was 88.03%, and the cure rate was 95.07%6 months post surgery.In the group of TVT, I-QOL and P-ISQ score were 52.1 ±16.8 and 60.1 ±36.5 at time of pre-operation, and they were 92.1 ±17.1 and 62.2 ±37.8 at time of post-operation, respectively.In the group of TVT-O, they were 61.2 ±15.8 and 57.1 ±36.8 at time of pre-operation, and they were 91.3 ±20.1 and 62.1 ±35.9 at time of post-operation, respectively.Compared with pre-operation, I-QOL and P-ISQ score were obviously rised at time of post-operation ( P<0.05 ). The total incidence rate of surgery complication was 21.83%.And,in proper was urinary tract infection (16.9%),OAB (14.08%), bladder injury(11.26%), retention of urine(7.74%), nerve pain(2.81%), retropubic hematoma(1.41%), urethral injury(1.41%).All patients′surgeries were successful except three cases.One patient was cured after reoperation .The other two was improved with dislodge sling urinary incontinence . Conclusion TVT/TVT-O was a highly effective and safe method in the treatment of SUI .But we should not ignore the complications;the timely and effective treatment measures could improve the operation rate of success .

Hypoxia is a general characteristic of most solid malignancies and intimately related to cancer progression. Homeostatic response to hypoxia is primarily mediated by hypoxia inducible factor-1alpha (HIF-1alpha) that elicits transcriptional activity through recruitment P300 coactivator. Targeting the interaction of HIF- alpha and P300 would thus constitute a novel approach for cancer treatment by suppressing tumor angiogenesis and metastasis. Here, a screening assay was developed for inhibitors targeting the interaction between HIF-1alpha and P300. The nucleotide sequence of human HIF-1alpha and P300 were cloned into pBIND and pACT vectors, named pBIND-HIF1alpha and pACT-P300. The interaction of HIF-1alpha and P300 was identified in HEK293 cell using mammalian two-hybrid system. And compound chetomin decreased their interaction in this mammalian two-hybrid system. We further verified HIF-1 inhibition effect of chetomin in U251-HRE cells. Therefore, we established a screening assay combined HIF-1alpha and P300 mammalian two-hybrid system and U251-HRE reporter assay for HIF-1 selective inhibitors.

Objective To investigate the effect of DHA on proliferation and killing activity of γδT cells against SW1990 cells in vitro.Methods γδT cells were generated in vitro by stimulating peripheral blood mononuclear cells of healthy donors in RPMI 1640 completed medium containing IPP and IL-2 for 8 d,and then co-cultured with different concentrations of DHA for 48 h.Proliferation rates of γδT cells for each group were detected by MTT method.The perforin,granzyme B and CD107a expression in γδT cells were verified by flow cytometer.The cytotoxic activity of γδT cells against SW1990 cells were analyzed by CCK-8 kit.Results The purity of γδT cells in each group reached 75.46％ ±5.32％ after 8 d of culture.Compared with the control group,the proliferative capability of γδT cells were enhanced significantly after treated with 50-100 μmol/ml DHA for 48 h,moreover,cytotoxicity against SW1990 cells and perforin,granzyme B and CD107a expression of the γδT cells treated with DHA were higher than the control group.Conclusion DHA could enhance the antitumor activity of γδT cells,which may be associated with the upregulation of perforin and granzyme B expression in γδT cells.