Animals ; Dietary Supplements ; Electron Transport ; Hypoxia ; Iron ; pharmacology ; Liver ; Mitochondria, Liver ; drug effects ; Rats

Adult ; Anti-Bacterial Agents ; adverse effects ; Female ; Humans ; Laryngeal Diseases ; etiology ; microbiology ; Mycosis Fungoides ; etiology ; Prescription Drug Misuse ; Substance-Related Disorders ; complications ; Young Adult

Objective To study the changes of serum and ascitic procalcitonin (PCT ) level in the patients with liver cirrhosis complicating spontaneous bacterial peritonitis (SBP) and to investigate the value in its early diagnosis .Methods 92 patients with cirrhotic and ascites were detected the levels of serum and ascitic PCT by the immunofluorescence method and the detection results were compared with the ascites routine and the bacterial culture results .Results Serum levels of PCT in the SBP group and the non-SBP group were obviously higher than those in the control group ,the difference was statistically significant (P<0 .05);Serum and ascitic levels of PCT in the SBP were significantly higher than those in the non-SBP group ;The sensitivity of serum PCT for di-agnosing peritonitis was 89 .3% ,the sensitivity of ascitic PCT for diagnosing peritonitis was 71 .4% .Conclusion The levels of ser-um and ascitic PCT have the important value for the early diagnosis of liver cirrhosis ascites ,moreover serum PCT level has higher value for early diagnosing liver cirrhosis ascites .

Adolescent ; Constriction, Pathologic ; Female ; Fibromatosis, Aggressive ; complications ; pathology ; Humans ; Pharyngeal Diseases ; etiology ; pathology

The therapy to different type of breast cancer has resulted in chemoresistance which is induced by auto-phagy.Autophagy, to tumor’s generation, is a double edged sword, and it can result to chemoresistance and auto-phagic cell death through interaction with apoptosis in breast cancer chemotherapy.However, autophagy may be unrelated to chemoresistance.It will be a key research point to further explore the relationship and mechanism be-tween autophagy and chemoresistance.

BACKGROUND:How to preserve the biological properties and osteogenic activity of bone xenograft materials after antigen removal has become one of the hot spots. OBJECTIVE:To investigate the effect of bone xenograft extract on proliferation, migration and osteogenic differentiation of bone marrow mesenchymal stem cel s. METHODS:Bone marrow mesenchymal stem cel s from Sprague-Dawely rats were cultured respectively in bone xenograft extract (experimental group) and DMEM/F12 medium containing 10%fetal bovine serum (control group). MTT, Transwel chamber and flow cytometry were used to detect cel proliferative ability, migration ability and cel cycle changes. Passage 3 bone marrow mesenchymal stem cel s were selected in the two groups and cultured in osteogenic induction medium. At 7 and 14 days of osteogenic induction, alkaline phosphatase staining was done;at 21 days of osteogenic induction, alizarin red staining was performed to observe calcium nodule formation. RESULTS AND CONCLUSION:After 1, 3, 5, 7 days of culture, the cel proliferative activity and cel cycle had no significant difference between the two groups, but the number of migrating cel s was significantly lower in the control group than the experimental group (P<0.05). At 7 and 14 days of culture, the alkaline phosphatase level was significantly higher in the experimental group than the control group (P<0.05);at 21 days of culture, calcium nodes formed in both two groups, but the calcium nodule area was larger in the experimental group than the control group. These findings indicate that bone xenograft materials have no cytotoxicity but possess good cytocompatibility, which can promote the proliferation, migration and osteogenic differentiation of bone marrow mesenchymal stem cel s.

Objective To investigate the relationship between motor abnormality of sphincter of Oddi(SO) and hepatolithiasis.Methods The hepatolithiasis rabbit models were established in adult rabbits(n=64) which were randomly divded into 2 groups:control group(n=16)and experimental group(n=48),including 2 wk,4 wk and 8 wk group(n=16 per group).During the formation process of hepatolithiasis,bile character and ratio of stone formation were both observed,then the myoelectric waves of SO were recorded.Finally,The SO segment was sliced and observed by transmissional electron microscope.Results The rate of true stone formation in group 8 wk(68.75%,11/16)was higher than that in group 4wk(12.50%,2/16)and in group 2wk(0.00%,0/16).Compared with the control group,there was significant decrease of both SO myoelectric peak amplitude and SO myoelectric frequency in the experimental group.Electron microscopy showed that SO cells in control group and 2wk group had no change,but the 4wk and 8wk groups underwent changes including fibrolysis of myofilament,increase of collagen fibers,fusion and thickening of microfilament,decrease and array disorder of macula densa,swelling of chondrosome and endoplasmic reticulum,widening of perinuclear space and deviation and pyknosis of nucleus,and formation of inclusion body in cytoplasm;and the degeneration degree in 8wk group was obvious than that in group 4wk.Conclusions The functional abnormality of SO may contribute important effects on the formation of hepatolithiasis.Fibrous degeneration of SO maybe the pathogenesis of functional abnormality of SO.

Objective To investigate the expression and significance of vascular endothelial growth factor(VEGF) in the various types of degenerated lumbar discs and the normal control.Methods Forty-one cases of degenerated intervertebral disc tissue(the contained group with 17 cases and uncontained group with 24 cases) from surgical intervention for lumbar disc herniation and 13 cases of normal disc tissue from anterior approach for scoliosis and lumbar fracture were stained with hematoxylin and eosin.The expression of VEGF was tested by immunohistochemistry method.Results There was no capillary infiltration and VEGF positive expression in the control group.The VEGF positive rate was 41.2%(7/17) in the contained group,and 79.2%(19/24) in the uncontained group.In the contained group and uncontained group,the VEGF positive rate was higher than that in the control group(P

Objective To summarize the recent progress in pathogenetic,diagnostic and therapeutic researches on the intestinal barrier dysfunction(IBD) of severe acute pancreatitis(SAP).Methods The advancement of IBD in SAP,which was published recently at home and abroad,was collected and reviewed.Results The pathogenesis of IBD in patients with SAP was complex.Ischemia-reperfusion injury,endotoxin,inflammatory mediators and gastrointestinal hormone played an important role in the process of IBD.There were many ways to detect IBD,and the ratio of lactulose and mannitol,plasma diamine oxidase were relatively ideal markers.Medical therapies,such as treatment of SAP and maintaining the perfusion of intestines,were essential to cure IBD.On this basis,the propulsives,nutritional support and traditional Chinese drugs should be administered reasonably.Conclusions IBD is a sophisticated process of pathophysiology.In recent years,abundant of animal experiments and clinical researches have provided new clue for prevention and cure of IBD,but further researches are still needed on the mechanism of the cells and molecules implicated.