Objective To explore the best plan of perioperative individualized fluid therapy in the elderly patients with coronary heart disease undergoing gastrointestinal surgery.Methods Eighty elderly patients with coronary heart disease undergoing gastrointestinal surgery were divided into experimental group and control group by random digits table method with 40 cases each.Conventional capcity treatment was given during surgery and postoperative period in control group.Individualized fluid therapy was used during surgery and postoperative period in intensive care unit of 24 h in experimental group,which was determined based on target controlled fluid therapy according to the cardiac index,stroke volume,and stroke volume variation.Traditional fluid therapy was used in control group in the intraoperative and postoperative period.The two groups were compared in terms of postoperative hemodynamic parameters,total fluid volume,incidence of adverse cardiac events,and recovery of gastrointestinal function.Results The mean arterial pressure at the beginning of the operation,the cardiac index at operation begining 1 h and at the end of operation were (80.4 ± 6.4) mmHg (1 mmHg =0.133 kPa),(4.0 ± 0.6) L/(min ·m2),(4.1 ± 0.8) L/(min ·m2) in experimental group and (76.9 ± 8.0) mmHg,(3.5 ± 0.4) L/(min· m2),(3.6 ± 0.3) L/(min · mè) in control group,and there were significant differences between two groups (P ＜ 0.05).During surgery and 24±hour stay in intensive care unit,the total fluid volume,crystal usage,urine and colloid usage were (2 915 ± 650),(2 715 ± 415),(1 515 ± 315),(1 225 ± 385),(419 ± 233),(619 ± 285),(1 015 ± 220),(1 535 ± 455) ml in experimental group and (3 645 ±770),(3 355 ±750),(2 315 ±320),(1 855 ±325),(602 ±31),(875 ±423),(805 ±250),(1 135 ± 205) ml in control group,and there were significant differences between two groups (P ＜ 0.05).The perioperative adverse cardiac events rate was 30％ (12/40) in experimental group,lower than 45％ (18/40) in control group,but the difference was no statistically significance (P ＞ 0.05).Conclusion In the elderly patients with coronary artery disease undergoing gastrointestinal surgery,individualized fluid therapy can effectively decrease adverse cardiac events,improve postoperative gastrointestinal function,and reduce length of hospital stay.

Major infectious diseases affect human health seriously .In order to prevent , control and treat diseases effectively , the key lies in rapid detection and identification of pathogens .Rapid development and wide application of molecular biological techniques , such as PCR, gene sequencing and bio-chip technology , have greatly improved the prevention , control and treatment of major infectious diseases .The future development trend is to establish an accurate , rapid, high-throughput , portable and intelligent diagnostic technology of molecular biology .

HBV infection is the key factor that causes liver inflammation,liver injury and immune dysfunction.Chronic hepatitis caused by HBV,a non-cytotoxic virus,is a kind of immune injury induced by liver cells that clear virus infection in immune system.Effector T lymphocyte plays a central role in immune system to clear HBV in liver cells,and the outcome of HBV infection is directly influenced by its functions and quantity.After antiviral therapy for patients with sustained HBV infection,whether to restore and rebuild immune function is critical in achieving ideal end point of treatment.Technologies,mainly including stimulation and culture of HBV antigen,flow cytometry,enzyme-linked immunoassay to detect cytokines,etc,are used in detection of HBV-specific T lymphocytes.

Hepatitis B virus has a high degree of genetic variation. Multiple selection pressure,exerted by vaccine,antiviral drugs and diagnostic reagents,might favor certain mutations of HBV. HBV variation under the selective pressure may affect the pathogenesis of HBV-related liver disease,immunization effect,antiviral efficacy and laboratory tests.In this paper,we summarize the molecular characteristics and its significance of HBV variants,which will help us to manage hepatitis B.

According to the large-scale investigation on the positive rates of serum HBV markers in hospital population, the HBV infection rate in Chinese population went down gradually and the infection rate of HBV was markedly lower in the low age people. Applying epidemiological principle to design,technology standardization,multiple-center survey were the premise of reliable data on HBV infection rate in hospital population, which could be used as important basis of decision making on prevention and control.

Statement of the importance of the basic legal principles for both the law and law operation,demarcation of the scope of the basic principles for the general health law,and establishment of the legal interests under its protection.The paper proposes that the cornerstones of the general health law should observe the three basic principles of protection of citizens' healthy life,improvement of health conditions,and safeguard public health and safety,with the three clearly stipulated in the law.

Objective:To investigate the effect of the endocrine disruptor chemicals(EDCs) on female workers' reproductive system function and the correlation between female reproductive disorders and EDCs.Methods:All 2 893 subjects are female workers of a factory in Chongqing,with 1 652 as exposed group and 1 241 as control group.The data were derived from the gynecological examination.Results:The results showed that the rate of abnormal menstruation in exposed group(14.8%) was significantly(P

Objective:To construct a prokaryotic expression plasmid of PQE30-IL3-Linker-PE38KDEL and identify its recombinant protein expression.Methods:The IL3 and PE38KDEL gene were amplified by polymerase chain reaction(PCR) and cloned into the prokaryotic expression plasmid PQE30-Linker constructed after being sequenced.The recombinant vector confirmed by restriction endonucleases digestion,coenobium PCR,and DNA sequence analysis was transformed into E.coli SG13009.The expression of the protein was induced by IPTG.Relative molecular weight of the expression product was detected by SDS-PAGE.Finally,the fusion protein was examined by Western blot.Results:The results of restriction endonuclease digestion,coenobium PCR and DNA sequence analysis showed that the prokaryotic expression vector PQE30-IL3-Linker-PE38KDEL was constructed successfully.With induction of IPTG,the relative molecular weight of the expression product was identical to the expected value.The expressed 6?His-IL3-PE38KDEL fusion protein were identified at relative molecular mass of 57KD by Western blot with anti-His monoclonal antibody,showing the fusion protein expressed correctly.Conclusion:The fusion protein IL3-PE38KDEL is successfully constructed,which lays a solid foundation for the further research of protein purification and function.

Childbearing, for women, is a great event in life, and because of having little knowledge of it, ill psychological elements such as anxiety as well as dread always occur in primipara, thus midwifery and service skills should meet higher requirements. Therefore, doing good humanistic nursing is a realistic problem confronting all obstetric nursery personnel. This article, just from the aspects of transforming nursing concepts and renewing service model, tries to interpret the superiority and inevitability of humanistic obstetric service.

Adult ; Cyclooxygenase 2 ; metabolism ; Esophageal Neoplasms ; diagnosis ; metabolism ; Female ; Humans ; Pregnancy ; Receptor, Epidermal Growth Factor ; metabolism ; Vascular Endothelial Growth Factor A ; metabolism ; Young Adult