ObjectiveTo observe the effect of probucol combined with atorvastatin in treatment of stenosis carotid atherosclerosis.MethodsEighty-six patients with stenosis carotid atherosclerosis were separated into two groups with 43 cases each by random digits table.The patients were treated with probucol combined with atorvastatin in observation group and atorvastatin only in control group.The changes of arteriosclerotic plaque and blood fat pre- and poet-treatment were observed.Results There was no significant difference in the levels of lumen area (LN) and intima-media thickness (IMT) pre-treatment between two groups (P＞ 0.05).Compared with pre-treatment,the levels of LN and IMT post-treatment were significantly decreased in observation group(P ＜ 0.05 ),while no significant difference in control group (P ＞0.05).There was significant difference in the levels of LN and IMT post-treatment between two groups [(0.59 ±0.19) cm2 vs.(0.72 ±0.28) cm2,(2.76 ±0.41 ) mm vs.(3.27 ±0.57) mm](P＜ 0.05).There was no significant difference in the levels of total cholesterol (TC),triacylglycerol (TG),low density lipoprotein cholesterol (LDL-C),high density lipoprotein cholesterol (HDL-C) pre-treatment between two groups (P ＞0.05).Compared with pre-treatment,the levels of TC,TG,LDL-C post-treatment were decreased,HDL-C was increased,there were significant differences (P ＜ 0.05 ).Post-treatment,the levels of TC,TG,LDL-C in observation group were lower and HDL-C was higher than those in control group (P ＜ 0.05).Conclusion Probucol combined with atorvastatin in treatment of stenosis carotid atherosclerosis can improve arteriosclerotic plaque and low blood fat,which can be applied in clinic.

Chemokine is a small protein which plays an impor-tant role in men's physiological function.It has chemotactic ac-tivity and is often secreted by immune cells and glial cells like microglia or astrocytes.Through the effect of chemokine recep-tors on target cells,various immune cells can achieve directional migration and play an important role in the diseases related with immunity and inflammation.CCL2,also known as monocyte chemotactic protein-1 (MCP-1 ),is one member of chemokine CC subfamily (βsubfamily).It can chemokine monocytes, macrophages and T lymphocytes to affect their phagocytosis func-tion and produce antibodies to combat invading microorganisms. In recent years,it has been found that CCL2 plays a key role in the occurrence and development of the problems concerning cen-tral nervous system and immune system as well as cancer, AIDS,leukemia,diabetes and other diseases.This thesis is to give an elaboration on the latest research on CCL2 and the rele-vant diseases.

Objective To study the expression site and the role of low-affinity nerve growth factor receptor(P75) in hepatic stellate cells(HSCs) of fibrotic human and rat liver.Methods Left lobe of liver was removed from CCl-4-induced rats fibrosis model and human fibrotic tissue sample was taken from liver puncture.The tissue specimen was routinely paraffin embedded and expression of P75 was detected by histochemical staining using polyclonal antibody against P75.Additionally,HSCs was cultivated and P75 was immunochemically assayed for P75 binding activity. Results P75 was seen abundantly on cell membrane of the cultured HSCs.In the fibrotic tissue of rat liver as well as human fibrotic liver P75 was also positively expressed.Similarly,P75 was found mainly a hepatocye surface in histochemical staining.P75 was not seen by histochemical staining in the normal tissue of human and rat liver.Conclusion P75 used as a new target for the treatment of hepatic fibrosis.

Objective To study the influence of serum containing Ziqi Ruangan Pill on inhibitory effect of alcohol LO2 and to investigate the regulation on apoptosis related genes and the protective mechanism for LO2. Methods Rabbit sera saline containing Ziqi Ruangan Pill was prepared. The apoptotic LO2 model was established by being cultured with alcohol. The effect of serum on the model was detected by MTT method. Identification of apoptosis was carried out using flow cytometry and Hoechst3325 fluorescent staining. The expression of apoptotic inducing gene,casepase3 and NF-?B was detected by Real-time RT PCR and Western-Blot. Results The serum containing Ziqi Ruangan Pill could regulate the expression of the casepase3,NF-?B and reduce the apoptosis. Conclusion Ziqi Ruangan Pill can protect the LO2 by regulating the expression of apoptosis stimulative gene casepase3 and NF-?B.

Objective:To make a synthetic comment on treatment of children cerebral palsy with acupuncture in recent 10 years in China.Methods:Related papers were gotten from the journals between 1999-2008 in Chinese journal full-test database and VIP information-Chinese technology journal database by computer retrieval.The collected 143 papers were analyzed from the terms of clinical acupoints selection,treatment methods,time,and mechanism,and then summarized the clinical effects of acupuncture in treating children cerebral palsy.Results:The acupoints selection was focus on scalp acupuncture functional area and body points(including scalp points).Beside acupuncture treatment,the combination of acupoint embedding therapy,acupoint injection,and massage were also used.The therapy time was different from 20min to 4h,and the treatment course was usually be counted by month.The effects of acupuncture treatment were considered to be significant through improving brain circulation and recovering brain cells' function. Conclusion:Acupuncture treatment had advantages in improving the child'limbs function,nevertheless further studies on the research methods,study design and mechanism were all needed.

Radiation therapy plays an important role in the multidisciplinary treatment of early stage breast cancer. While breast conservative treatment has become an established strategy, recent clinical investigations focus mainly on the possibility of modification to the standard practice, including the significance of tumor bed boost after whole breast irradiation, the criteria for low-risk patients not to receive adjuvant radiotherapy after breast conservative surgery and the feasibility of partial breast irradiation. Post-mastectomy radiotherapy to the chest wall and regional lymph nodes in high-risk patients has been proved to reduce the local-regional recurrence by 2/3. However, only a few trials support the improvement of survival, and significant controversy exists concerning the role of post-mastectomy radiotherapy in intermediate-risk patients. Current review summarizes the advances and controversies stated above for the treatment of early stage breast cancer.

Embryonic stem cell possess multi-directional differentiation capacity and can differentiate into all sorts of cells of three germ layers,it has the widely use in the treatment of tissue repair.Recent study shows that,embryonic stem cells can differentiate into cartilage cells in the effect of cytokines,hormones,micro-environment etc.In the is review,the author explore several studies and review the effects of different factors to the differentiation of embryonic stem cell to chondrocyte.

WIF-1 is an important antagonist in the Wnt/?-catenin pathway.As a tumor suppressor, WIF-1 is down-regulated in majority cancers but when it is correlated with WIF-1 promoter hypermethylation and treated with demethylation drug, its expression can be restored.Functional loss of WIF-1 due to promoter hypermethylation plays an important role in a tumor's pathogenesis, possibly mainly through aberrant canonical Wnt/?-catenin signal activation.The correlation of the expression of WIF-1 with clinicopathological features in different cancers is not identical.Detecting WIF-1 and promoter hypermethylation may offer help for early diagnosis, therapy and predicting prognoses.

The curriculum for general practitioner training does not include much knowledge on medical humanities except for medical ethics,which can not meet the requirement of general practitioners' defined duty.This article analyzes the necessity of adding social psychological skills to the training and assessment of general practitioners from the following aspects of the nature of general practitioners' duty,and the disadvantages of medical humanities education in current Chinese medical universities.Then the proposition of this system is also presented.

Objective To study the effects of ligustrazine(LTZ) on the preeclampyic umbilical serum induced-expression of precollagen I,III in cultured human umbilical artery smooth muscle cell(HUSMC). Methods The cultured HUSMC was treated with LTZ for 30 min, and then incubated with medium containing 20% serum either from women with preeclampsia or normal pregnant women for 48h. The cell activity was determined by MTT, the cell cycle was analyzed by flow cytomerty, and RT-PCR analysis was used to dectect the expression of precollagen I,III. Results The cell proliferation, percentage of S and G2/M phases, and expression of precollagen I obviously increased in HUSMC incubated with preeclampsia umbilical serum compared with normal pregnant women one(P