Acupuncture Therapy ; instrumentation ; methods ; Aged ; Female ; Humans ; Male ; Middle Aged ; Periarthritis ; physiopathology ; therapy ; Range of Motion, Articular ; Shoulder Joint ; physiopathology ; Treatment Outcome

Objective To clone and express Schistosoma j aponicum cyclophilin B (SjCyPB) gene in E.coli,and to identify and analyze the immunity of recombinant proteins.Methods A pair of specific primers was designed according to GenBank of Schistosoma japonicum sequence.SjCyPB gene was amplify by PCR and then connected to pET28 vector.The recombinant plasmid pET28a (+)-SjCyPB was constructed and transformed into E.coli BL21 cell line,the recombinant plasmid was identified by double enzyme digestion and sequence analysis.After induced by isopropyl-B-D-thiogalaetoside (IPTG),the expressed recombinant proteinwas purified by affinity-chromatography,and then verified by Western blotting.Rats were immunized recombinant SjCyPB,andthe SjCyPB-specific IgG was detected by ELISA.Results SjCyPB gene was successfully inserted into pET28a(-) vecter which identified by double enzyme digestion and sequence analysis.Recombinant SjCyPB protein was highly expressed in E.coli.The Western Blotting analysis confirmed that the recombinant protein could specifically combine to S.japonicum-infected rabbit serum.Using recombinant protein to immunize rats,the SjCyPB-specific IgG antibody titer was 1 ∶ 51 200 detected by ELISA.Conclusion The recombinant SjCyPB is successfully constructed,and recombinant SjCyPB has immunogenicity and antigenicity.

Objective Liver is a common site of metastasis of gastric cancer and hitherto there has been no uniform treatment for liver metastasis of gastric cancer. This study was to investigate the clinical characteristics and prognostic factors of liver metastasis of gastric cancer in order to provide some evidence for the improvement of the diagnosis and treatment of the disease. Methods We ret-rospectively analyzed the clinical characteristics and survival data of 143 patients with liver metastasis of gastric cancer treated in our hospital between March 2007 and September 2012. We investigated the independent risk factors affecting the prognosis of the disease u-sing the Cox Regression Model. Results Seventy-five ( 52. 4%) of the 143 patients survived for 12 months, 41 ( 28. 7%) for 24 months, and 18 (12.6%) for 36 months, with a median survival time of 14 months. Univariate analysis revealed statistically significant differences in the survival time among the patients with different histological grades of primary gastric cancer, time of liver metastasis, types of metastatic liver cancer, extra-liver metastasis, and treatment methods ( P<0.05) . Multivariate Cox regression analysis showed the histological grade of gastric cancer to be low-or non-differentiation ( HR=2.67, 95% CI:1.51-4.72) and the type of liver meta-static cancer to be H2 ( HR=1.89, 95% CI:1.08-3.29) or H3 ( HR=2.13, 95% CI:1.32-3.44) , which were independent risk fac-tors affecting the prognosis of liver metastasis of gastric cancer, while palliative chemotherapy ( HR=0.55, 95% CI:0.32-0.95) was an independent protective factor of its prognosis. Conclusion Patients with liver metastasis from gastric cancer usually have a poor prognosis, especially those with histologically low-grade gastric cancer or type-H2 or -H3 liver metastatic cancer. The prognosis of thepatients undergoing palliative chemotherapy may have a better prognosis than those receiving best supportive care. A comprehensive therapy should be recommended to patients with liver metastasis from gastric cancer.

Spinal cord injury (SCI) is a common traumatic disease. SCI causes nutritional and metabolic abnormalities including malnu-trition and overnutrition. This article reviewed nutritional metabolism assessments, the changes and reasons after SCI. Spinal Nutrition Screening Tool (SNST) and Malnutrition Universal Screening Tool (MUST) are commonly used to evaluate malnutrition in adult. Screening Tool for the Assessment of Malnutrition in Paediatrics (STAMP) is used to evaluate malnutrition in child. Body mass index, waist circumfer-ence and dual-energy X-ray are used to evaluate overnutrition. SCI causes increasement of total caloric intake, disturbance of carbohydrate metabolism, disturbance of fat and protein metabolism, increasement of metabolic syndrome incidence, high risk of coronary heart disease, and abnormal bone metabolism. Individual diet program should be provided to SCI patients for balanced nutrition. Periodic assessments are also necessary.

Objective To study the vaginal microenvironment in pregnant women with preterm premature rupture of the membranes,and to explore the change of vaginal microenvironment in pregnant women with preterm premature rupture of the membranes.Methods 1 16 women with preterm premature rupture of the membranes were selected as preterm premature rupture of the membranes group,1 16 women with normal pregnancy outcomes were selected as control group.The vaginal pH value,vaginal lactobacilli,vaginal sialidase and HD5,IgE and SIgA levels were observed.Results At 28 weeks of pregnancy,the vaginal PH value,sialidase detection rate and HD5,IgE,SIgA levels of preterm premature rupture of the membranes group were higher than those of the control group,but there were no significant differences between the two groups(t=1.037,χ2 =1.052,t=1.282,1.051,1.861,all P>0.05).At pregnant 36 weeks,the vaginal pH value,sialidase detection rate and HD5,IgE,SIgA levels of preterm premature rupture of the membranes group[(4.13 ±0.22),71.4%,(12.53 ±1.55)pg/mL,(338.84 ±1.67)ng/mL, (53.44 ±1.57)μg/mL]were higher than those of the control group[(3.79 ±0.25),13.8%,(8.77 ±3.17)pg/mL, (167.43 ±3.66)ng/mL,(37.59 ±1.85)μg/mL],the differences were statistically significant(t=12.331,χ2 =24.816,t=5.258,9.469,7.357,all P<0.05).At 28 weeks of pregnancy,the vaginal lactobacilli detection rate of preterm premature rupture of the membranes group was lower than that of control group,but there was no significant difference(χ2 =1.723,P>0.05).At 36 weeks gestation,the vaginal lactobacilli detection rate of preterm premature rupture of the membranes group was lower than that of the control group,the difference was statistically significant (χ2 =19.545,P<0.05).Conclusion Preterm premature rupture of the membranes pregnant vaginal pH value increases,the detection rate of vaginal lactobacilli decreases,vaginal sialidase detection rate increases,vaginal immune factors HD5,IgE,SIgA level increases.

Objective:To analyze and compare the effect of integration of Traditional and Western Medicine treatment and western treatment for patients with severe acute pancreatitis.Methods:According to the including criteria,nineteen randomized controlled trials of this topic were enrolled into the analysis.The detail about the trial design characters of the subjects, results of the studies were reviewed and analyzed by Revman 4.1 software.Results:Compared with integration of Traditional and Western Medicine treatment,western treatment was associated with a significantly higher incidence of mortality(RR=0.35,95%CI 0.25～0.48,P

The mercury content in soil and sediment, although minimal, but its toxicity is substantial.Different speciation of mercury has different toxicity, so the determination of total mercury can not fully reveal its toxicity and bioavailability.The speciation of mercury has become more and more indispensable.This paper summarizes the extraction and determination methods of the total and the speciation mercury in soil and sediment, and the extraction and determination of methyl mercury.

Objective To compare the clinical efficacy of three different concentrations of ropivacaine combined with morphine and granisetron on postoperative patient controlled analgesia by epidural anesthesia.Methods Ninety postoperative patients were randomly divided into three groups with 30 cases each.The patients in group Ⅰ received 0.25% ropivacaine,in group Ⅱ 0.20% ropivacaine,in group Ⅲ 0.125% ropivacaine.Each group combined with morphine(6?g/ml) and ciranisetron(0.03mg/ml).The patients controlled epidural analgesia(PCEA) continually infused of 2ml/h.Motor block(in limb) was assessed with Bromage scale.Results Visual analogue scales(VAS) were observed at 6,12,24 and 48h postoperatively.Pain score was higher in group Ⅲ than that in group Ⅰ and Ⅱ.Motor block by Bromage scale was higher in group Ⅰ than that in group Ⅱ and Ⅲ.Each group had no anesthetic complication.Conclusion PCEA by 0.20% ropivacaine combined with morphine and granisetron can provide a good postoperative analgesia and have no significant motor retarded.It is worth popularizing.

AIM: To study the effects of oxidized high-density lipoprotein (oxHDL) on the expression of monocyte chemoattractant protein-1(MCP-1) and intercellular adhesion molecule-1(ICAM-1) and intracellular free calcium concentration （［Ca2＋］i） level in cultured human umbilical venous endothelial cells(HUVECs). METHODS: The MCP-1 protein content in the medium of conditioned HUVEC was measured by ELISA, and the ICAM-1 on HUVECs was detected by indirect immunofluorescence, and ［Ca2＋］i was determined by Fluo-3/AM, the injury of cells was observed by scanning electron microscopy (SEM).RESULTS: oxHDL could induce the expression of MCP-1 and ICAM-1 in HUVECs. In oxHDL group (HUVECs were incubated with 100 mg protein/L oxHDL for 24 h), the levels of MCP-1, ICAM-1 and ［Ca2＋］i increased by 160%, 60% and 70% respectively compared with the control group (P＜0.01). When HUVECs were incubated with 300 mg protein/L oxHDL for 24 h, cells were injured obviously. CONCLUSION: By inducing the expression of ICAM-1 and MCP-1 in endothelial cells, oxHDL may promote monocyte-endothelium adhesion and monocyte migration to intima, it may promote atherosclerosis as oxidized low-density lipoprotein (oxLDL).