Objective To investigate the effect of tonic pain stimulation on the attention network test (ANT) . Methods Twenty healthy males recruited from university were enrolled in this study, and laboratory pain was induced by cuff pressure stimulation on the upper arm. All subjects performed the task separately under pain, no-pain and pressure conditions. The reaction time and accuracy rate of the ANT were quantified. The subjective pain perception was assessed by the short-form McGill Pain Questionnaire ( SF-MPQ) . Results Across three conditions, the executive network showed significant pain effect (F = 76.31 , P< 0.001) : no-pain condition at (92.51 ±35.71 )ms, pressure condition at (94.06 ±25. 28) ms, pain condition at ( 120.24 ± 18.21 )ms. Furthermore, the average reaction time and the A-scores (i. e. affective words) in SF-MPQ caused by pain was positively correlated ( r = 0.11, P = 0.03) . Conclusion Pain can result in deficit in the executive network for attention regulation. The negative affections caused by pain can interfere with attention.

AIM: To study the effects of oxidized high-density lipoprotein (oxHDL) on the expression of monocyte chemoattractant protein-1(MCP-1) and intercellular adhesion molecule-1(ICAM-1) and intracellular free calcium concentration （［Ca2＋］i） level in cultured human umbilical venous endothelial cells(HUVECs). METHODS: The MCP-1 protein content in the medium of conditioned HUVEC was measured by ELISA, and the ICAM-1 on HUVECs was detected by indirect immunofluorescence, and ［Ca2＋］i was determined by Fluo-3/AM, the injury of cells was observed by scanning electron microscopy (SEM).RESULTS: oxHDL could induce the expression of MCP-1 and ICAM-1 in HUVECs. In oxHDL group (HUVECs were incubated with 100 mg protein/L oxHDL for 24 h), the levels of MCP-1, ICAM-1 and ［Ca2＋］i increased by 160%, 60% and 70% respectively compared with the control group (P＜0.01). When HUVECs were incubated with 300 mg protein/L oxHDL for 24 h, cells were injured obviously. CONCLUSION: By inducing the expression of ICAM-1 and MCP-1 in endothelial cells, oxHDL may promote monocyte-endothelium adhesion and monocyte migration to intima, it may promote atherosclerosis as oxidized low-density lipoprotein (oxLDL).

Objective To evaluate the effect and safety of transobturator inside-out tension-free urethral suspension (TVT-O) as the surgical treatment on post-operational recurrence of female stress urinary incontinence (SUI). Methods Eleven women with SUI recurrence who underwent TVT-O were selected in the research. Their incontinence recurred from 1 months to 8 years after the previous surgical treatment.Results The operative time was (28 ±9) min, the bleeding volume was (15 ±5) ml. Postoperative complications: urinary retention in 1 case, vaginal erosion in 1 case. Follow-up was enforced for 6-36months, median time was 16 months. Grouts-Blaivas analogue scale:among the 11 patients, 9 patients were cured, 2 patients were improved. ICI-Q-SF score: preoperative 13-21 ( 15.36 ± 2.34) scores compared to postoperative 0-9 (1.46 ±3.27) scores (t =5.850,P ＜0.01 ). I-QOL score: preoperative 23.33-36.67(29.85 ± 4.74) scores compared to postoperative 66.67-100.00 (95.15 ± 10.12) scores (t = 17.753,P ＜0.01 ). PISQ-12 score of 10 patients: preoperative 16-25 (20.10 ± 3.07) scores compared to postoperative 11-20( 14.00 ± 2.83 ) scores (t = 11.600, P ＜ 0.01 ). Conclusion If conservative treatments fail, TVT-O is highly effective and safe for the postoperative recurrent SUI.

SUMO (small ubiquitin-related modifier) is involved in the post-translational modifications of proteins,and this process is referred to as SUMOylation.SUMOylation plays an important role in the regulation of cellular activities such as strengthening the stability of the protein,nucleocytoplasmic transport,DNA repair,DNA replication,mitotic and meiotic chromosome behavior,et al.SUMOylation is a dynamic process and can be reversed by SUMO-specific proteases (SENP).Once the balance between SUMOylation and de-SUMOylation is broken,there will be an aberrant expression of SUMO or SENPs in cells to happen,which may lead to the tumor occturtrence.

Objective To study the vaginal microenvironment in pregnant women with preterm premature rupture of the membranes,and to explore the change of vaginal microenvironment in pregnant women with preterm premature rupture of the membranes.Methods 1 16 women with preterm premature rupture of the membranes were selected as preterm premature rupture of the membranes group,1 16 women with normal pregnancy outcomes were selected as control group.The vaginal pH value,vaginal lactobacilli,vaginal sialidase and HD5,IgE and SIgA levels were observed.Results At 28 weeks of pregnancy,the vaginal PH value,sialidase detection rate and HD5,IgE,SIgA levels of preterm premature rupture of the membranes group were higher than those of the control group,but there were no significant differences between the two groups(t=1.037,χ2 =1.052,t=1.282,1.051,1.861,all P>0.05).At pregnant 36 weeks,the vaginal pH value,sialidase detection rate and HD5,IgE,SIgA levels of preterm premature rupture of the membranes group[(4.13 ±0.22),71.4%,(12.53 ±1.55)pg/mL,(338.84 ±1.67)ng/mL, (53.44 ±1.57)μg/mL]were higher than those of the control group[(3.79 ±0.25),13.8%,(8.77 ±3.17)pg/mL, (167.43 ±3.66)ng/mL,(37.59 ±1.85)μg/mL],the differences were statistically significant(t=12.331,χ2 =24.816,t=5.258,9.469,7.357,all P<0.05).At 28 weeks of pregnancy,the vaginal lactobacilli detection rate of preterm premature rupture of the membranes group was lower than that of control group,but there was no significant difference(χ2 =1.723,P>0.05).At 36 weeks gestation,the vaginal lactobacilli detection rate of preterm premature rupture of the membranes group was lower than that of the control group,the difference was statistically significant (χ2 =19.545,P<0.05).Conclusion Preterm premature rupture of the membranes pregnant vaginal pH value increases,the detection rate of vaginal lactobacilli decreases,vaginal sialidase detection rate increases,vaginal immune factors HD5,IgE,SIgA level increases.

Object To investigate the pathological changes,physiological condition,neurobehavior and motor development of the 3-day-old rats with ischemic brain injury.Methods Ninety six 3-day-old male Sprague-Dawley rats were randomly assigned to experimental and control groups.Occlusion of both carotid arteries was performed in the experimental group,while the rats in the control group only received skin incisions without carotid ligation.Physical examinations and neurobehavioral development of the rats were recorded daily from the first day after operation until 3 weeks after birth,including weight,eye opening, incisor eruption,ear unfolding,righting reflex,negative geotaxis,limb placing and grasping reflex. Specimens of the brain tissue were obtained in 24 hours after operation and 3 weeks after birth for the hematoxylin-eosin staining and immunohistochemistry staining to investigate the pathological changes.All the reaults were compared between the 2 groups.Results Compared with the control group,rats in the experimental group were found growth retardation,suppression of primitive reflexes and impaired motor abilities (P < 0.05 ).The brain tissue obtained from the rats after operation showed white matter rarefaction,liquefaction and microglia hyperplasia with Hematoxylin-eosin staining and myelin formation disorder with immunohistochemistry staining.Conclusions Ischemic brain injury of the 3-day-old rats could result the mental retardation,neurobehavioral and motor development disorder because of the white matter injury.

Objective To clone and express Schistosoma j aponicum cyclophilin B (SjCyPB) gene in E.coli,and to identify and analyze the immunity of recombinant proteins.Methods A pair of specific primers was designed according to GenBank of Schistosoma japonicum sequence.SjCyPB gene was amplify by PCR and then connected to pET28 vector.The recombinant plasmid pET28a (+)-SjCyPB was constructed and transformed into E.coli BL21 cell line,the recombinant plasmid was identified by double enzyme digestion and sequence analysis.After induced by isopropyl-B-D-thiogalaetoside (IPTG),the expressed recombinant proteinwas purified by affinity-chromatography,and then verified by Western blotting.Rats were immunized recombinant SjCyPB,andthe SjCyPB-specific IgG was detected by ELISA.Results SjCyPB gene was successfully inserted into pET28a(-) vecter which identified by double enzyme digestion and sequence analysis.Recombinant SjCyPB protein was highly expressed in E.coli.The Western Blotting analysis confirmed that the recombinant protein could specifically combine to S.japonicum-infected rabbit serum.Using recombinant protein to immunize rats,the SjCyPB-specific IgG antibody titer was 1 ∶ 51 200 detected by ELISA.Conclusion The recombinant SjCyPB is successfully constructed,and recombinant SjCyPB has immunogenicity and antigenicity.

Objective Liver is a common site of metastasis of gastric cancer and hitherto there has been no uniform treatment for liver metastasis of gastric cancer. This study was to investigate the clinical characteristics and prognostic factors of liver metastasis of gastric cancer in order to provide some evidence for the improvement of the diagnosis and treatment of the disease. Methods We ret-rospectively analyzed the clinical characteristics and survival data of 143 patients with liver metastasis of gastric cancer treated in our hospital between March 2007 and September 2012. We investigated the independent risk factors affecting the prognosis of the disease u-sing the Cox Regression Model. Results Seventy-five ( 52. 4%) of the 143 patients survived for 12 months, 41 ( 28. 7%) for 24 months, and 18 (12.6%) for 36 months, with a median survival time of 14 months. Univariate analysis revealed statistically significant differences in the survival time among the patients with different histological grades of primary gastric cancer, time of liver metastasis, types of metastatic liver cancer, extra-liver metastasis, and treatment methods ( P<0.05) . Multivariate Cox regression analysis showed the histological grade of gastric cancer to be low-or non-differentiation ( HR=2.67, 95% CI:1.51-4.72) and the type of liver meta-static cancer to be H2 ( HR=1.89, 95% CI:1.08-3.29) or H3 ( HR=2.13, 95% CI:1.32-3.44) , which were independent risk fac-tors affecting the prognosis of liver metastasis of gastric cancer, while palliative chemotherapy ( HR=0.55, 95% CI:0.32-0.95) was an independent protective factor of its prognosis. Conclusion Patients with liver metastasis from gastric cancer usually have a poor prognosis, especially those with histologically low-grade gastric cancer or type-H2 or -H3 liver metastatic cancer. The prognosis of thepatients undergoing palliative chemotherapy may have a better prognosis than those receiving best supportive care. A comprehensive therapy should be recommended to patients with liver metastasis from gastric cancer.

The effects of intravenous injection of purifed EC on the rectal tempera-ture in normal rabbits and febrile rabbits induced by ET and concentrations of Fe~(2+), Zn~(2+),Cu~(2+) in serum were observed. The results showed that EC could lower the rectal tempera-ture in normal rabbits and also the increased rectal temperature in febrile rabbits. Mean-while, EC could inhibit the formation of the second phase of ET-fever. But EC had noeffect on the changed concentrations of FC~(2+), Zn~(2+), Cu~(2+) in serum caused by ET. Thesefindings suggest that EC may particiate in the regulation of body temperarure under physio-logical and febrile conditions, but not of acute phase response produced by ET.

AIM: To investigate the effects of oxidized high-density lipoprotein (oxHDL) on membrane fluidity and the expression of lymphocyte function associated antigen(LFA-1) of monocyte. METHODS: The membrane fluidity of THP-1 cells was assayed by fluorescence anisotropy with DPH (1,6-dipheny-1,3,5-hexatriene), a fluorescent probe; The LFA-1 expression on THP-1 cells were assayed by flow cytometry with indirect immunofluorescence.RESULTS: The membrane fluidity of THP-1 cells was reduced by 45% and 52% respectively ( P