Objective To compare the reliability of acute physiology and chronic health evaluation Ⅱ (APACHE Ⅱ) and APACHE Ⅲ to estimate mortality of critical patients in abdominal surgery. Methods Two hundred and sixty-one critical patients in abdominal surgery were included in this study. The clinical data of the first day in ICU were collected and evaluated with both APACHE Ⅱand APACHE Ⅲ prognostic systems and statistical analysis were performed. Probability of survival (Ps) was compared with actual mortality. Results The scores of APACHE Ⅱ and APACHE Ⅲ of death group were significantly higher than those of survival group respectively (P

OBJECTIVE:To investigate the preventive use of antibacterials in patients undergoing cesarean section in our hospital.METHODS:A total of 360 medical records of patients undergoing cesarean section were randomly sampled in 2006(30 cases per month)for an analysis of the correlation between choice of antibacterials,combination rate and course of treatment etc and curative effects.RESULTS:Of the 360 cases,86.8% used second and third generation cephalosporins,6.3% fourth generation cephalosporin(cefepime),3.8% cephradine.CONCLUSION:No positive correlation was noted between choice of antibacterials and course of treatment and the curative effects in the prevention of postoperative infection.

Objective The hippocampus has been used in Chinese medicine for a long time.Although it has the anti-decrepitude,anti-fatigue and anti-tumor effect,the molecular mechanism is not yet known.PI4-K,the first protein kinase in the PI pathway,should be the target molecular for drug screening.Methods We prepared antigen protein IOV-PI4-K through inside-out ghost,then the antibody-antigen reaction between IOV-PI4-K and its antibody A6D was tested by ELISA.Results Two different function groups,which could increase or decrease the binding affinity of PI4-K to its antibody,were screened out from the extracts of hippocampus.This fact indicated that there might be two different components to up-regulate or down-regulate the activity of PI4-K respectively.Conclusion Derivatives of the components could be developed into anti-tumor or anti-decrepitude drugs.Meanwhile,a new way for performing drug screen through ELISA was established.

Urethral stricture is one of the common complications after hypospadias repair,and it is related with the blood supply of materials used in urethra formation.The more severe and proximal hypospadias is,the higher possibility of stricture is.The patients with urethral stricture have the symptoms of stranguria and urinary retention,and they usually need re-surgery.The repair with well vascularized flap can decrease the occurrence of urethral stricture after hypospadias repair.

Using brartemicin as the leading compound, fifteen novel trehalose derivatives were designed and synthesized, and the structures were determined by 11H NMR, MS and element analysis. Inhibitory effects of the target compounds on the proliferation of A549, HepG2 and HUVEC cells were detectec by MTT assay. The abilities of adhesion, invasion and migration of A549 and HepG2 cells inhibited by the synthesized compounds were evaluated through Matrigel experiment and Transwell assay. The results showed that, the target compounds had no significant cytotoxicity (compared with the control, P>0.05) to A549, HepG2 and HUVEC cells at the dose range of 1-32 µmol.L-1. At the above dose range, the inhibitory effects of A549 cells adhesion, invasion and migration and HepG2 cells adhesion and invasion by compounds 79 and 82 are better than brartemicin.
Antineoplastic Agents ; chemical synthesis ; pharmacology ; Cell Adhesion ; Cell Line, Tumor ; drug effects ; Drug Design ; Hep G2 Cells ; drug effects ; Human Umbilical Vein Endothelial Cells ; drug effects ; Humans ; Trehalose ; analogs & derivatives ; chemistry

Using brartemicin as the leading compound, fifteen novel trehalose derivatives were designed and synthesized, and the structures were determined by 11H NMR, MS and element analysis. Inhibitory effects of the target compounds on the proliferation of A549, HepG2 and HUVEC cells were detectec by MTT assay. The abilities of adhesion, invasion and migration of A549 and HepG2 cells inhibited by the synthesized compounds were evaluated through Matrigel experiment and Transwell assay. The results showed that, the target compounds had no significant cytotoxicity (compared with the control, P>0.05) to A549, HepG2 and HUVEC cells at the dose range of 1-32 µmol.L-1. At the above dose range, the inhibitory effects of A549 cells adhesion, invasion and migration and HepG2 cells adhesion and invasion by compounds 79 and 82 are better than brartemicin.

Objective On the basis of previous researches,the paper confirmed the weight of indicators to build emergency physicians'competency models for their different career stages.Methods Using the method of analytic hierarchy process (AHP)to establish evaluation hierarchy for emergency physicians'different career stage and construct the pair wise comparison judgment matrix,checking consistency and judgment matrix at the same time.Results Achieved the construction of competency models for three different stages of emergency physicians,primary,intermediate and advanced included.Conelusion The models can dynamically clarify the career stages of emergency physicians,supporting personalized training and management of the physicians.

Objective To evaluate the effects of gender on the median effective target plasma concentration (EC50) of propofol inhibiting responses to laryngeal mask airway (LMA) insertion in patients.Methods Fiftyfive ASA physical status Ⅰ or Ⅱ patients,aged 20-60 yr,with body mass index 18-25 kg/m2,requiring LAM insertion,undergoing elective surgery,were divided into 2 groups according to the gender:male group (group M,n =27) and female group (group F,n =28).Propofol was given by target-controlled infusion.Anesthesia was induced with target-controlled infusion of propofol.The concentration of propofol was determined by using modified Dixon's up-and-down method.The initial target plasma concentration of propofol was 3.0 μg/ml (group M) or 2.7μg/ml (group F) and the ratio between the 2 successive concentrations was 1.1.Fentanyl 1 μg/kg was injected intravenously at about 10 min of propofol target-controlled infusion.LMA was inserted after the effect-site and plasma concentrations were balanced.The positive response to LMA insertion was defined as development of swallowing,coughing,biting LMA,body movement and laryngospasm during insertion.When the response to LMA insertion was positive,the concentration of propofol was increased in the next patient and when the response was negative,the concentration was decreased in the next patient.The EC50 and 95 ％ confidence interval of propofol inhibiting responses to LMA insertion were calculated.Results The EC50 (95 ％ confidence interval) of propofol was 3.11 (2.92-3.30) μg/ml in group F and 2.70 (2.55-2.85) μg/ml in group M (/ ＜ 0.05).Conclusion The EC50 of propofol inhibiting responses to LMA insertion is higher in female patients than in male patients.

Kawasaki disease (KD) is a systemic vasculitis diseases.It may be involved multisystem.The most serious complication is coronary artery lesions,which is associated with coronary atherosclerosis in adult.Coronary artery disease caused by endothelial cell dysffunction has attracted the attention of many scholars.Now summarize the research progress in recent years as follows:endothelial cell function evaluation methods,KD markers of endothelial cell injury,and the relationship of the endothelial cell dysfunction with KD coronary lesions.