Objective To evaluate the clinical practical value of the heart rate viability test in the patients with malignant tumor during their therapies. Methods Totally 187 cases were analyzed and were divided into three different groups, which included 90 malignant tumor patients before therapy, 45 patients with chemotherapy and 52 non-malignant tumor cases (control group). We compared four HRV indexes(ASDNN5,SDANNS, SDNN and RMSSD) of the patients between the different groups as well as inside each group. Results There were statistical significances in the indexes of ASDNN5, SDANN5 and SDNN of patients at different stages before therapy, the three indexes of the stage Ⅲ group were lower than those of the stage Ⅰ group and the control group as well. The three indexes of the stage Ⅳ group compared with the stage Ⅰ group or the control group (P<0.05) were lower either. There was also statistical significances in SDNN between the stage Ⅲ and the stage Ⅱ of the before-therapy group (P< 0. 05). The indexes of SDANN5 and SDNN of the stage Ⅳ in the chemotherapy group were lower than those of the same stage in the before-therapy group(P<0.05). Conclusions The later the stage of malignant tumor patients is, the more the index of HRV declines. Chemotherapy may affect autonomic nerve function of stage Ⅳ patients with malignant tumor and decrease their HRV.

10 units allows identification of patients a high risk factors in development ARDS.The high risk fac- tors providing the greatest contribution to improve therapy in acute lung injury and prevent ARDS to occur.

BACKGROUND: At the process of wound healing, the epithelial cells and epidermal stem cells interactions is complicated which promoting the mechanisms of wound healing. The abnormities of any fine phase can be result in delayed healing or scarring. OBJECTIVE: To summarize the characterization of epithelial cells and epidermal stem cells and their interaction in wound healing so that we can recognize the mechanisms of scarring from histomorphology, biochemistry and molecular variations. METHODS: A computer-based online search of Pubmed Database was undertaken to identify the relevant articles on epithelial cell and epidermal stem cells interactions in wound healing and results in scarring published from 1974 to 2009 with the key words of "epithelial cells, epithelial stem cells, wound healing, keratinocytes, fibroblasts, hypertrophic scar, keloid" in English. At the same time, Chinese relevant articles were searched in China Journal Full-text Database (CJFD) published between 1999 and 2009 with the same key words in Chinese. A total of 43 articles were collected about the cell structure (3 articles), the cell interactions in wound healing and scarring (16 articles), the relevant on epithelial stem cells (24 articles).RESULTS AND CONCLUSION: The effect of different cells in epithelial tissue is known to all. The keratinocytes and fibroblasts had been on studying frequently, and their closely correlated to scarring. At present, the epithelial stem cells has been confirmed, but the relevant on their location, quality and specific epidermal markers are unclear which need to study and explore. With the rapidly development of science and technology, we maybe control the expression of cells by genic regulation not only culture the cells of our need but to prevent the scarring.

Brain ; physiology ; Humans ; Magnetic Resonance Imaging ; Mastication ; physiology

Evidence-based medicine (EBM) has rapidly developed in recent years and is currently a hotspot in international medicine. The guiding principle behind EBM is that any medical decision should be determined based on objective clinical and scientific evidence, doctor's professional skills and experience, and patient's needs and desires. This paper introduces the concept and practice of EBM and explains how it can be used to solve the issues in stomatology.
Evidence-Based Medicine ; Humans ; Oral Medicine

Objective:To study the significance of detection o f lymph node micrometastas is by immunohistochemical method with monoclonal anti-karatin antibody in patie nts with colorectal cancer.Methods:We examined 331 lymph nodes obtai ned from 47 colo rectal cancer patients who underwent a curative operation and diagosed by using immunohistochemistry SB techniques.Monoclonal mouse anti-keratin antibodies (AE1,AE1/AE3,AE3)were used as the primary reagents to identify tumor cells both in tumor tissues and lymph nodes.Results:A total of 36 micrometa stasis among 331 lymph nodes(11.9%) were found.Duke's C staging patients had more lymph nodes m etastasis than that in Duke's B staging patients(P＜0.05).Postoperative fol low-up revealed that recurrence rate was high and the latent period of distant metastasis was much shorter in patients with occult mirometastasis th an those without.Conclusions:Even in histologically negative lym ph nodes,there i s a considerable possibility that micrometastasis may exist.The immunohistochemical detection of monoclonal anti-keratin antibody can explica te clinical staging a nd as an indication for colorectal cancer therapy.

Aim To explore the modulation of Salvia miltiorrhiza on hyperpolarization-activated current (Ih) channels in dorsal root ganglion (DRG) neurons of rats and identify the mechanism of Salvia miltiorrhiza in alleviating pain and inhibiting calcium overload. Methods The effect of Salvia miltiorrhiza injection on Ih channels in DRG neurons of rats were examined by using whole-cell patch clamp technique. Results The experimental results showed that the amplitude of Ih evoked by -150 mV was (-1.06±0.18) nA. The Ih could be fitted well into the single kinetics and the time constant of activation, τ was clearly voltage-dependent with τ=(322.14±28.81) ms at -100 mV, decreasing to τ=(62.51±9.78) ms at -150 mV. The reversal potential of Ih was (-35.03±1.12) mV measured from tail currents. But no significant differences were found between the DRG neurons in the absence and presence of Salvia miltiorrhiza injection (10%, 25%, 50%) in the current amplitude, the time constant of activation and the reversal potential. The only difference between the DRG neurons in the absence and presence of Salvia miltiorrhiza injection was the half-activation potential of Ih. In control recordings the half-activation potential was (-106.07±3.59) mV. By comparison, the half-activation potentials changed to (-111.59±3.79) mV (n=31 neurons, P＜0.05), (-119.37±4.96) mV (n=31 neurons, P＜0.05) and (-121.23±3.86) mV (n=31 neurons, P＜0.05) in the presence of 10%, 25%, 50% Salvia miltiorrhiza injection, respectively. Conclusion Only the half-activation potential of Ih in the arthritic and neuropathic rat models shifted in the depolarizing direction, which increased the electrophysiological activity of Ih and made it related to peripheral hyperalgesia. The selective inhibition of Salvia miltiorrhiza on the electrophysiological activity of Ih may be one of the mechanisms underlying its analgesic effects.

BACKGROUND:After cerebral ischemia/reperfusion injury,as a executioner caspase, procaspase-3 and caspase-3-like activity increased significantly. We observe both the dephosphorylated and phosphorylated procaspase-3, and try to find out their variations during the processes of cerebral ischemia/reperfusion injury.OBJECTIVE:Observe the expression of procaspase-3 in hippocampus following transient forebrain ischemia.DESIGN: A randomized and controlled experiment.DEPARTMENT:Department of Biochemistry and Molecular Biology,Capital University of Medical Sciences.SETTING:Department of Hyperbaric Oxygen of Beijing Chaoyang Hospital Affiliated to Capital University of Medical Sciences.METHODS:Transient forebrain ischemia was induced by bilateral common carotid occlusion (BCCAO) for 20 minutes. Hippocampus was obtained at reperfusion time points of 6 hours, 12 hours,24 hours and 48 hours respec-tively after 20 minutes of BCCAO. Sham-operated group did not occlude bi-lateral common carotid, and hippocampus was obtained at reperfusion time point of 24 hours. Western Blotting was used to detect the level of procaspase-3.MAIN OUTCOME MEASURES:The comparison of total procaspase-3,dephosphorylated procaspase-3 and phosphorylated procaspase-3 level in hippocampus between each group.3 level: Total procaspase-3 level increased in hippocampus at reperfusion time points of 12 hours and 24 hours post-BCCAO (9 133.1 ±2 216.3,dephosphorylated procaspase-3 level increased in hippocampus at reperfusion time point of 24 hours post-BCCAO (7812.0±1625.1, 3825.8±155.6, P was not significant (P ＞ 0.05) as compared with the expression levels in sham-operated mice.CONCLUSION: Procaspase-3 is upregulated after ischemia/reperfusion.The increment of dephosphorylated form of procaspase-3 was higher than that of phosphorylated form of procaspase-3 upon cerebral ischemia/reperfusion injury, which indicates that cerebral ischemia/reperfusion injury possibly induced the dephosphorylation of procaspase-3 and promoted its transforming into activated form.

Severe acute pancreatitis (SAP) is a critical disease which has a high mortality. In order to make the diagnosis and treatment much more promptly, rationally and effectively, this article briefly introduces some methods for assessment of the severity and risk of SAP by clinical manifestation, risk factors,scoring systems, radiology and laboratory examinations.

In HCl medium,trace o-dinitrobenzene can sensitively inhibit the discoloration reaction of neutral red oxidized by KIO4. The optimum neagent is 0.55 mL neutral red, 0.60 mL HCl, 0.6 mL KIO4, respectively. The neaction ttmperatune is 80℃， neaction time is 6 min. The optimum condition and kinetic parameters are investigated in detail,a new method for determination of trace o-dinitrobenzene is proposed.　The　detection limit is 7.7×10-7　g/L and the linear range is 0.0～4.0　μg/L o-dinitrobenzene.　It has been　applied to the determination of trace o-dinitrobenzene in environmental water sample with　satisfactory results.