AIM: To establish a HSP90 highly expressing cell line and study the effect of high level of HSP90 on cell stress response. METHODS: The recombined plasimid pSmycHSP, which contains the full length DNA coding for human HSP90?, was introduced into mouse fibroblast cell line NIH-3T3 by electroporation after being subcloned, purified and identified by limited enzyme digestion. Screened by G418, the positive clones were selected and identified by immunofluorescence, immunocytochemistry and Western-blotting. NIH-3T3 cells transfected with empty plasmid served as control, hyperthermia(44 ℃, 20 min, 40 min)was used to simulate oxidative stress. The activity of lactate dehydrogenase (LDH) in supernatant and damage of DNA were detected by automatic biochemistry analyzer and flow cytometer separately to analyze the effect of high-level HSP90 on cell membrane and DNA injuries under stress condition. RESULTS: The rising level of HSP90 was shown by immunofluorescence, immunocytochemistry and Western-blotting in HSP90 overexpressing cell line. There was no difference in the leakage of LDH between HSP90 overexpressing cell line and control, but the damage of DNA was more severe at 44 ℃for 20 min in HSP90 overexpressing cell line than control. Compared with control, the above indices were relieved at 44 ℃ for 40 min in HSP90 overexpressing cell line. CONCLUSION: The NIH-3T3 derived cell line, which stably expressed high level of HSP90, was established. The effect of high-level HSP90 on cells is complex at different intensity of stress, and the protection may be shown at more severe stress circumstance.

Objective To elucidate the effect of early enteral nutrition support(ENS) for the patients with severe acute pancreatitis(SAP).Methods Fifty patients with SAP were at random divided into two groups: enteral nutrition group(EN group, 25 cases), parenteral nutrition group(PN group, 25cases). PN group was given total parenteral nutrition(TPN),125 kJ/(kg?d) of energy, nitrogen intake:0.20g/(kg?d). In EN group, the naso intestinal tube was placed posterior to ligament Treitz. Since the 1st day nutrilion was infused.Results On the first?4th day, the serum RBP?PA levels were obviously decreased (P

Objective To explore the value of multi-slice spiral CT (MSCT) with three dimensional (3D) postprocessing for assessing the lung volume and compare it with pulmonary function test (PFT). Methods In 60 cases, there were 34 healthy controls and 26 patients with emphysema. CT scan and PFT were performed within 3 d in all case. The total lung was respectively scanned at the end of full inspiration and full expiration, using GE Lightspeed 16 slices spiral CT. The total lung volumes and voxel index (VI) at threshold range of -1023 Hu to -900 Hu were measured by pulmonary quantitative software of AW4.2 workstation. Results The full expiration volume(Vex) significantly correlated with residual volume(RV) (r=0.908) as well as the full inspiration volume (Vin) with total lung capacity (TLC) (r=0.897). The value of (Vin-Vex) well correlated with forced vital capacity(FVC), as well as Vex/Vin with RV/TLC, VI Ain with TLC, VI Aex with RV. Conclusion Significant correlation of lung volume accessed by MSCT with 3D technique with PFT is useful for assessing the pulmonary function.

Objective To analyze the causes of poor compliance of elderly patients with diabetes mellitus , and put forward corresponding countermeasures to control blood glucose level and delay the occurrence of complications .Methods A total of 113 elderly patients with type 2 diabetes mellitus aged 60 years and over were recruited in endocrinology department of our hospital.The self-made questionnaire was used to investigate the compliance and the factors that affected the compliance.Results There were 48 patients (42.48%) with good compliance and 65 patients (57.52%) with poor compliance.Univariate analysis showed that the level of education, monthly income, drug types, understanding of diabetes-related knowledge and whether the elderly living alone affected compliance ( P<0.05 ) .Multivariate Logistic regression analysis showed that patients with low education level, poor monthly income, medication types and living alone were the main factors that influence the compliance of elderly patients with diabetes mellitus (P<0.05).And the level of education, monthly income level and the compliance of diabetic medication was positively correlated, medication types and living alone and diabetes compliance were negatively correlated (P<0.05).Conclusion The low level of education, poor monthly income, medication types and living alone are the main factors that affect the compliance of elderly patients with diabetes.The social and family support can effectively improve the compliance of elder patients with diabetes.

The hypoxic pulmonary vasoconstriction (HPV) in Yunan monkey has been observed. The results showed that acute alveolar hypoxia caused significant pulmonary hypertension, associated w~th art increase in pulmonary vascular resistance. The maximal response appeared 3 min after onset of hypoxia. Monkey may be proposed as moderate responder in HPV. The HPV in monkey was blocked by lipoxygenase inhibitor diethylcarbamazine and enhanced by cyclooxy genase blocker...indomethacin. It is suggested that lipoxygenase metabolites leukotrienes probably play the role of a mediator in HPV of monkey, whereas cyclooxygenase metabolites prostaglan dins act as medulator.

0.05],but patients with grade 2 and 3 had significantly lower Plasma CO levels,[(15.4?1.4),(12.5?1.7)vs(21.1?2.4)mmol/L,P

Objective To study the the effectiveness of Entecavir on serum transforming growth factor-β1 (TGF-β1) in patients with decompensated cirrhosis .Methods Eighty patients with decompensated cirrhosis were randomly divided into two groups ,the control group(n=40 cases) and the observation group (n=40 cases).The pa-tients in the control group were treated through the conventional treatmeat ,while the patients in the observation group were treated through the conventional treatment plus entecavir .They were treated for 24 weeks.Liver function,HBV DNA and serum TGF-β1 were detected.Results ALT,Tbil and ALB were improved after treatment in both groups (t=6.724,13.306,3.064,4.172,2.924,3.172,all P<0.05).They were more significantly improved in the obser-vation group than that in the control group (t=2.469,2.160,3.064,all P<0.05).HBV-DNA negative rate in the observation group was higher these in the control group (χ2 =4.82,P<0.05).Serum TGF-β1 in both groups were decreased after treatment(t=6.842,8.062,all P<0.01).There was a significant difference between two groups (t=3.178,P<0.05).Conclusion Entecavir can decrease serum TGF-β1 in patients with decompensated cirrhosis .

Objective To evaluate the expressions and roles of survivin (SVV), bcl-2 and proliferating cell nuclear antigen(PCNA) in cutaneous squamous cell carcinoma (SCC). Methods The expressions of SVV, bcl-2 and PCNA were investigated by SP immunohistochemistry technique in 82 cases of SCC and 18 normal skin samples. Results There were 79.3% of SCC patients with the expression of SVV (P

OBJECTIVE:To further exploit the active components from Loquat leaf and its gruffs.METHODS:The chief components in loquat leaf and its gruffs were extracted by ultrasound extraction and Soxhlet extraction,respectively,the sepa?ration of ursolic acid and other constituents was performed by HPLC,and the relative amounts of which were determined and compared.RESULTS:Soxhlet extraction is superior to ultrasound extraction in the extraction of loquat leaf and8components in gruffs.Under the same extraction method,the content of ursolic acid in loquat leaves is equivalent to that of gruffs.Besides,the contents of6components in gruffs were above those in loquat leaves.CONCLUSION:The essential component-triterpenic acid in loquat leaves suffered no loss after extraction with water,the active components in gruffs can be further exploited.

OBJECTIVE To compare the detection rates of Ureaplasma urealyticum(Uu),by 4 kinds rits and find out the best one.METHODS Using four kinds of mycoplasma detection kits,namely are French mycoplasmaIST2,Zhuhai Livzon′s ICS,Dark horse′s IES and Shanghai Its Prachanda,under the same conditions,the mycoplasma of the Clinical specimens for 260 cases were tested.RESULTS The detection rate of mycoplasma(Uu) for 48 hours by four kinds of brands kits about mycoplasma liquid culture was as follows 45.4% for French MycoplasmaIST2,36.2% for Zhuhai Livzon′s ICS,37.7% for Dark horse′s IES and 36.8% for Shanghai Its prachanda.The results of susceptibility test: the reagent of IST2 had.the lowest resistance to josamycin,doxycycline,clarithromycin,ofloxacin and azithromycin,and had the better results than the other three kinds of reagents.Mycoplasma reagents form different companies had significant differences in resistance rates to five kinds of antibiotics.CONCLUSIONS The results suggest that when we cultivate clinical mycoplasma should be careful to select of the mycoplasma liquid culture identification kit.