[Objective] We summarizes the professor Shao Changrong's experience in treating COPD by using invigorating spleen and draining dampnessmethod. [Method] This paper analyzes the etiology, pathology, therapeutic principle and relevant case revealing about the COPD patients with insufficien-cy of the spleen and phlegmatic hygrosis. And we explain the Pro. Shao's empirical prescription cal ed Ping-Ke-Hua-Tan decoction. [Results] In his view, COPD attack and aggravation are related with phlegmatic hygrosis. The fundamental reason is the insufficiency of the spleen and the external reason is phlegmatic hygrosis. Its mechanism is mostly the blending of excessiveness and deficiency. [Conclusion] Theinvigorating spleen and draining dampnessmethod is effective in treating COPD. Pro. Shao Changrong's diagnosing and treating of COPD is worthy widely clinical reference and promotion.

Objective To investigate the clinical risk factors of diabetic ketoacidosis (DKA ) combined with elevated serum amylase(AMS). Methods Totally 263 patients with DKA were enrolled in our study. They were divided into 2 groups according to the level of AMS:DKA with normal AMS (DKA,n=188);DKA with elevated AMS(HAMS,n = 75 ). HAMS patients then were divided into two subgroups according to the diagnosis with acute pancreatitis(AP)(HAMS+ AP,n = 25 )and without AP (HAMS,n = 50 ). TG,FPG and AMS were measured and analyzed with univariate analysis and multivariate analysis. Results HAMS group patients had a ratio of 28.5％ with elevated AMS and 9.5％ with AP.HAMS group had higher FPG,TG,HbA1 c,Na+ ,osmolality,BUN and Scr than DKA group(P <0.05).The levels of FPG,TG,osmolality,BUN and the time of CO2 CP recovery in HAMS+AP subgroup were all significantly higher than those in HAMS patients without AP (P < 0.05 ). Correlation analysis showed that HAMS was positively correlated with TG,FPG,Na+ ,Osmolality and BUN(P <0.05).Logistic regression analysis showed that hypertriglyceridemia was the independent risk factor of DKA combined with HAMS(OR 1.244,P <0.01),and was the independent risk factor of DKA combined with AP(OR 1.211,P <0.05). Conclusion Hypertriglyceridemia is the related risk factor of DKA combined with HAMS and AP. This study provides a theoretical basis to early screen patients with hyperamylasemia in DKA.

Objective To study the rehabilitation for batches of the wounded suffering from the earthquake in hospital. Methods 91 cases of trauma occurring in the earthquake were reported. Results There was no dead and any complication happened. 90 cases could self-care as they discharged. Conclusion Multiple specialty rehabilitation would be effective on the wounded suffering from earthquake.

Objective To study the expression regulation of GATA-3 on Th2 cytokines secretions in rats lung infected by respiratory syncytial virus (RSV).Methods 20 SD rates were random divided into two groups: Control group (10 rats) and RSV-infected group(10 rats, the model was established by RSV intravasation).The airway responses were observed after seven week.Lung tissue of SD rats were stained with HE, pathologic examinations of lung tissue- stained were made.GATA-3 expressions in lung tissue were detected by western-blots, respectively.Interleukin-4 (IL-4) and interleukin-5 (IL-5) in lung homogenate were detected by ELISA.Results Lung biopsy from RSV-infected group showed typical inflammation under light microscope.Airway resistance in RSV- infected group was higher than control group.Compared with control group( 1.231 ±0.31 ), the expression of GATA-3 significantly increased in RSV-infected group (1.743 ±0.21)( t =15.427, P ＜0.01).The levels of IL-4[(142.3 ± 12.3)ng/L] and IL-5[(167.5 ±14.2) ng/L] increased in RSV-infected group [(69.2±11.6) ng/L, ( 86.6 ± 14.3 ) ng/L] ( P＜0.01 ).There was a positive correlation between protein levels of GATA-3 and IL-4 in lung tissues (r=0.864, P＜0.01 ), and a positive correlation between GATA-3 and IL-5 in lung tissues(r=-0.756, P＜0.01).Conclusion RSV-infection may up regulate the expression of GATA-3 in lung, which contributed to inflammation of Th2 type.

Pancreatic cancer is a highly malignant tumor of digestive tract which early diagnose is very difficult and has low rate of surgical resection of advanced pancreatic cancer. However, the rate of postoperative recurrence and metastas is high. Its prognosis is poor. To improve the prognosis of pancreatic cancer , it is necessary to improve its early diagnosis and effective prediction of postoperative recurrence and metastasis. In recent years, with the development of proteomics, the early diagnosis of pancreatic cancer and its early diagnosis of recurrence and metastasis was possible. Wsing proteomics technology for protein differences screening, isolation and identification is conductive to early detection of pancreatic proteome changes and establishment of the markers for early diagnosis, and recurrence and metastasis of pancreatic cancer.

Objective To investigate effectiveness of dexmedetomidine on preventing shivering of patients after spinal surgery.Methods A total of 78 cases with spinal surgery under anesthesia were randomly divided into study group(dexmedetomidine treatment) and the control group(saline treatment).Heart rate(HR)and mean arterial pressure were observed and recorded at 15,30,60 min after intubation,before and after extubation.Ramsay sedation score,chills grading,chills occur time and postoperative adverse reactions were recorded.Results In study group,HR were ((72.3 ± 5.2) time/min,(83.2 ± 4.5) time/min),mean arterial pressure were((72.7 ±5.0) mmHg,(90.3 ±7.9) mmHg) at before and after extubation,significant lower than that of control group((73.6 ±4.9) time/min,(88.9 ±4.9) time/min; (78.5 ±4.3) mmHg,(95.5 ± 14.9)mmHg;P＜0.05).The incidence of shivering (7.7％) in study group was significantly lower than the control group(30.8 ％,x2 =6.685,P =0.009).Ramsay sedation score at immediately,30 min,60 min into the PACU were (3.1 ± 1.0),(2.1 ± 0.4),(2.0 ± 0.3) respectively,higher than those of control group ((2.0 ± 0.4),(1.3±0.2),(1.1 ±0.3) ; F inter-group =12.543,P＜0.001;F inner-group =9.391,P ＜0.001;F interaction =5.972,P =0.023).The incidence of adverse reactions such as nausea and vomiting in the control group was higher than those of the study group(P =0.003),while the incidence of dry mouth in study group was higher (P =0.002).Conclusion Intraoperative dexmedetomidine can significantly reduce the incidence and degree of shivering in patients with orthopedic spinal surgery after general anesthesia.Dexmedetomidine is proved to be a more ideal drug to prevent shivering in orthopedic spine surgery after general anesthesia.

Translational medicine is an emerging medical research pattern,its connotation includes a two-way process with effective translation of the latest basic research results into the available clinical medical technologies and products,and give the feedback of the clinical medical problems to the laboratory.It's a kind of bidirectional research,namely Bench to Bedside and Bedside to Bench.Translational medicine is one of the medical science frontiers.In recent years,scientists around the world pay high attention to the clinical translational applications of research results of hepatobiliary surgery,and relevant research and regulations formulation have started under the leadership of government.Actually,translational medicine plays an important role in the success of hepatobiliary surgery in liver transplantation era till now.China is expected to be at the forefront in hepatobiliary surgery and translational medicine in the world in the near future.Chinese surgical circles should have the whole world in view,set in the worldwide development in translational medicine,accelerate the dissemination of ideas in-depth to improve the technology and curative effect.

This article introduced the principle of cuvette feed of the STA compact hemoglutination analyzer and solutions for its malfunction.

Objective To evaluate the clinical significance of K-ras mutation in plasma cell-free DNA from patients with colorec-tal carcinoma .Methods Plasma cell-free DNA in 212 patients with colorectal carcinoma were extracted and identified by Globin gene .K-ras mutation was amplified by RE-PCR and compared with results of pathological biopsy samples DNA .Results Plasma cell-free DNA were successfully obtained from 212 cases .But no plasma cell-free DNA were found in normal control .Out of 212 ca-ses ,34 cases showed K-ras mutation(16 .00% )in cell-free DNA ,and 33 cases showed positive in pathological biopsy samples DNA (15 .57% ) .It was found two kinds of K-ras mutation on code 12-GGT to GAT ;GGT to GTT .K-ras mutation was not related to tumors′position(χ2 =3 .34 ,P=0 .61);to differentiation degree(χ2 =2 .38 ,P=0 .29);to Dukes staging(χ2 = 2 .84 ,P=0 .38);to lymphoid nodes′metastasis(χ2 =0 .98 ,P=0 .37);to age(χ2 =1 .86 ,P=0 .39) .Conclusion Tumor-derived DNA can be detected in plasma of underlying cancer patients .For colorectal carcinoma patients ,detecting K-ras mutation of plasma cell-free DNA have the same clinical significance as biopsy samples .Moreover ,it′s more convenient and non-invasive .