Objective To investigate the clinical risk factors of diabetic ketoacidosis (DKA ) combined with elevated serum amylase(AMS). Methods Totally 263 patients with DKA were enrolled in our study. They were divided into 2 groups according to the level of AMS:DKA with normal AMS (DKA,n=188);DKA with elevated AMS(HAMS,n = 75 ). HAMS patients then were divided into two subgroups according to the diagnosis with acute pancreatitis(AP)(HAMS+ AP,n = 25 )and without AP (HAMS,n = 50 ). TG,FPG and AMS were measured and analyzed with univariate analysis and multivariate analysis. Results HAMS group patients had a ratio of 28.5％ with elevated AMS and 9.5％ with AP.HAMS group had higher FPG,TG,HbA1 c,Na+ ,osmolality,BUN and Scr than DKA group(P <0.05).The levels of FPG,TG,osmolality,BUN and the time of CO2 CP recovery in HAMS+AP subgroup were all significantly higher than those in HAMS patients without AP (P < 0.05 ). Correlation analysis showed that HAMS was positively correlated with TG,FPG,Na+ ,Osmolality and BUN(P <0.05).Logistic regression analysis showed that hypertriglyceridemia was the independent risk factor of DKA combined with HAMS(OR 1.244,P <0.01),and was the independent risk factor of DKA combined with AP(OR 1.211,P <0.05). Conclusion Hypertriglyceridemia is the related risk factor of DKA combined with HAMS and AP. This study provides a theoretical basis to early screen patients with hyperamylasemia in DKA.

[Objective] We summarizes the professor Shao Changrong's experience in treating COPD by using invigorating spleen and draining dampnessmethod. [Method] This paper analyzes the etiology, pathology, therapeutic principle and relevant case revealing about the COPD patients with insufficien-cy of the spleen and phlegmatic hygrosis. And we explain the Pro. Shao's empirical prescription cal ed Ping-Ke-Hua-Tan decoction. [Results] In his view, COPD attack and aggravation are related with phlegmatic hygrosis. The fundamental reason is the insufficiency of the spleen and the external reason is phlegmatic hygrosis. Its mechanism is mostly the blending of excessiveness and deficiency. [Conclusion] Theinvigorating spleen and draining dampnessmethod is effective in treating COPD. Pro. Shao Changrong's diagnosing and treating of COPD is worthy widely clinical reference and promotion.

Objective To study the rehabilitation for batches of the wounded suffering from the earthquake in hospital. Methods 91 cases of trauma occurring in the earthquake were reported. Results There was no dead and any complication happened. 90 cases could self-care as they discharged. Conclusion Multiple specialty rehabilitation would be effective on the wounded suffering from earthquake.

OBJECTIVETo investigate the biuret reagent to detect proteins in the application, the impact of different test conditions for test results.

METHODSThe biuret method to select three different instruments, reagents, calibrators are arranged in combination to form 27 sets of detection systems, each detection system is a combination of 5 serum samples for testing, 5 measured values obtained, the selection process normality good a serum for the study to determine the mean value of all AST after culling outliers obtained in order to calculate the various detection systems use a combination of biuret reagent to detect proteins bias.

RESULTSThe use of different detection equipment to detect proteins biuret reagent bias, homogeneity of variance (P = 0.467), the difference was not statistically significant (F = 1.688, P = 0.421). different detection reagents using biuret reagent to detect proteins bias, homogeneity of variance (P = 0.574), a statistically significant difference (F = 5.784, P = 0.011). different calibrators use biuret reagent to detect proteins bias, homogeneity of variance (P = 0.467), the difference was statistically significant (F = 5.289, P = 0.000).

CONCLUSIONBiuret reagent in the detection of protein applications, impact detection reagents and calibrators will test result, during the test than when it is necessary to detect deviation detection reagents and calibrators due to be considered.

Objective To explore the relationship between health self-management skill and the severity of periodontitis disease.Methods 200 periodontitis patients (periodontitis group) and 200 health controls (control group) were assessed using the rating scale of health self-management skill for adults (AHSMSRS).Results (1) The total score of AHSMSRS in periodontitis group(139.08± 13.42),was lower than that in control group (177.51±16.16),and the difference was statistically significant (t=5.29,P＜0.01).(2) The number of different severity of periodontitis patients were mild 42 cases (21.0％),moderate 90 cases (45.0％),severe 68 cases (34.0％),respectively.Significant statistical differences were found in health self-management ability and each dimension (F=6.16-9.24,P＜0.01).Conclusion Compared with control group,health self-management skill of periodontitis patients is lower,and it may affect the condition of periodontitis.

Objective To review the advantages and disadvantages of common attention deficit hyperactivity disorder(ADHD) animal models,published in 2000-2014 at home and abroad,with an attempt to provide new ideas for the future study of the pathogenesis in ADHD.Methods Major online database including CNKI,Wan Fang databases,OVID,Pubmed databases were searched in January 2015,using the key wordsattention deficit hyperactivity disorder, animal modelsand so on,to analysis the advantages and disadvantages of each type of experimental animal models and research value in ADHD.Results Totally 29 studies were enrolled,and the analysis show that the current commonly used experimental animal models of ADHD are spontaneously hypertensive rats,dopamine transporter gene knockout mice,lack of mutant mice model,neonatal 6 hydroxy dopamine damage of juvenile rat model,neonatal hypoxic rats model,X-ray irradiation injury model of rat hippocampus and other brain tissue damage model and isolated feeding model,and each animal model has the advantages,disadvantages and special research value of the pathogenesis of ADHD.Conclusion The specific pathogenesis of ADHD is unclear,but the neuroendocrine changes especially HPA axis attracted much attention in recent years.this paper summarizes the research at home and abroad comparison of several common animal models of ADHD.neuroendocrine changes in SHR and ADHD children have a certain degree of consistency in HPA axis,looking for ADHD children and SHR in neuroendocrine aspects of consistency is likely to open a new path to search the pathogenesis in ADHD.

Objective To investigate the correlation between abnormal lipid metabolism and coronary artery lesions. Methods Two hundred and sixty seven patients were devided into the control group ( n =101) and the coronary heart disease (CHD) group ( n =166), which included single vessel lesion ( n =54), double vessel lesions ( n =68) and triple vessel lesions ( n =44) according to coronary angiography. At the same time the lipid components were examined and the ralationship between serum lipid level and coronary artery lesions were analysed. Results Compared with the control, the total cholesterol (TC), low density lipoprotein cholesterol (LDL-C) and triglyceride (TG) level were significantly elevated while but high density lipoprotein cholesterol (HDL-C) was lowered in the CHD group. The severity of coronary lesions was increased with the elevation of TC, LDL-C, apolipoproteinB (ApoB), TC/HDL-C, LDL-C/HDL-C, and the reduction of HDL-C. There were significant differences in terms of lipid level beteewn the CHD group and the control. The analysis showed that TC/HDL-C and LDL-C/HDL-C were most directly correlated with the coronary artery lesions in the CHD group. Conclusion The results suggest that the severity of coronary artery lesions correlated with abnormal lipid metabolism, and LDL-C/HDL-C, TC/HDL-C are better indicators than other lipid components.

Objective To observe the clinical effect of bisoprolol combined with simvastatin in the treatment of elderly patients with unstable angina pectoris.Methods 60 elderly patients with unstable angina pectoris were randomly divided into control group and study group according to the digital table method.The patients in the control group were treated with simvastatin,and the patients in the study group were treated with bisoprolol and simvastatin.The clinical therapeutic effect and adverse reactions of the two groups were compared.Results The total effective rate and the rate of ECG (86.67% and 80%)in the study group were significantly higher than those of the control group (76.67% and 46.67%)(χ2 =4.02,7.1 8,all P <0.05 ).The total incidence rate of adverse reactions (26.67%)in the study group had no significant difference compared with the control group (χ2 =0.08,P >0.05). Conclusion Bisoprolol combined with simvastatin had better clinical effect and small adverse reactions in the treat-ment of elderly patients with unstable angina.It was worthy of clinical use.

Simulating physiological neuronal and hormonal conditions during digestive and interdigestive periods, the study identified the changes of the motility of biliary system including bile duct and sphincter of Oddi (SO) before and after cholecystojejunostomy. Thirty-five rabbits were divided into five groups randomly. The experimental groups received the venous injection of CCK 10 ng/kg, erythromycin 10 mg/kg, atropine 3 micrograms/kg and L-NAME 10 mg/kg respectively. Each rabbit underwent manometry through introducing a three-lumen catheter via the papilla retrogradely, using the low-compliance papillary infusion system. Then the gallbladder and the upper segment of the jejunum was anastomosed and the manometric procedures repeated after one week. SO basal pressure was increased, contraction amplitude decreased, contraction time shortened after cholecystojejunostomy. L-NAME, CCK and erythromycin could all excite SO. L-NAME could increase basal pressure and contraction amplitude, CCK increase basal pressure contraction amplitude and frequency, and erythromycin increase contraction amplitude, respectively. But comparing with that before cholecystojejunostomy, the increasing extent was decreased. The tensional and spontaneous contractions of the SO were under the control of the neural and hormonal mechanism. The anastomosis of gallbladder and jejunum and the drainage of bile made the tensional contraction stronger, but the spontaneous contraction weakened after the operation due to the decreases of the sensitivity of SO to hormonal factors. The clinical symptoms may not be relieved when the patients with SO dysfunction accepted cholecystojejunostomy.
Cholecystectomy/*adverse effects ; Common Bile Duct/*physiopathology ; Gallbladder Emptying/*physiology ; Jejunostomy/*adverse effects ; Manometry/methods ; Muscle Contraction ; Postoperative Period ; Random Allocation ; Sphincter of Oddi/*physiopathology