Objective To classify all the plant pollen allergens, each allergen sequence available was compared with protein family database. After that, the appearance and taxonomic distribution of each protein family could be known. When made reference to evolutionary analysis, a regular rule of the distribu-tion of all plant pollen allergens could be concluded. Methods Protein family memberships of each allergen were assigned by comparing the sequences with the Pfam database. Representative members of the most a-bundant pollen allergen families were compared with the Uniprot database using the BLAST server. Acces-sion number of all the interesting homologous could be obtained and all the sequence information could be ac-quired by Batch Entrez. Finally, the evolutionary trees were drawn with the help of MEGA4.0 software. Re-sults One hundred and sixty-eight pollen allergens were classified into 26 protein families. Profilins, pollen _allerg_1 and EF hands constituted the most abundant pollen allergen families. Profilins and EF hands oc-curred in almost all allergenic plant families, whereas allergenic Expansins, FAD_binding proteins, Amb_V allergens and Thaumatins were confined to single taxon. Conclusion It is concluded that the highly con-served sequences of allergens families such as Profilin may be one of the most important reasons of the cross reactivities in allergic diseases. The classification of pollen allergens may be helpful to clinical practice and basic research.

Cardiomyopathy, Dilated ; etiology ; pathology ; therapy ; Humans ; Myocardium ; pathology

AlM:To compare the results of lOL Master, contact and immersion A-scan ultrasound measurements for anterior chamber depth ( ACD ) , and evaluate the difference and consistency.METHODS:Fifty-eight cases (98 eyes) with age-related cataract during July to October in 2013 did the A-scan ultrasound with contact and immersion measurements and lOL Master to get the results of ACD. Difference in measurements between methods was assessed using the variance analysisi. Consistency was assessed using Bland-Altman.RESULTS:The ACD measured by lOL Master was 2. 31~3. 90mm, the mean was 3. 03 ± 0. 38mm. The ACD measured by contact A- scan ultrasound was 1. 51 ~4. 06mm, the mean was 2. 88 ± 0. 56mm. The ACD measured by immersion A-scan ultrasound was 1. 99 ~4. 17mm, the mean was 3. 17±0. 38mm. The results of lOL Master and contact A - scan ultrasound had statistical differences (P=0. 022<0. 05). The results of lOL Master and immersion A - scan ultrasound had statistical differences (P=0. 031<0. 05). The results of contact A-scan ultrasound and immersion A-scan ultrasound had statistical differences (P=0. 000<0. 05). The consistency between three methods was poor. CONCLUSlON: The rank of ACD of patients with cataract is immersion A-scan ultrasound, lOL Master and contact A-scan ultrasound. The consistency is poor, and the three methods can’t be interchanged clinically.

Cell Growth Processes ; Hemangioma ; pathology ; physiopathology ; Histiocytoma, Benign Fibrous ; pathology ; physiopathology ; Humans ; Neoplasms ; physiopathology

Objective To investigate the incidence of abnormal thyroid function in pregnant women and explore the significance of thyroid function screening in early pregnancy. Methods We randomly selected 630 cases of pregnant women who received routine inspection in the obstetric clinic in our hospital, and gave them five items of thyroid function screening besides routine check at the same time. Then we statistically analyzed the test results, and followed up 61 cases of pregnant women with hypothyroidism or subclinical thyroid function (hypothyroidism) to learn the treatment and adverse pregnancy outcomes,so as tto explore the correlation between hypothyroidism, subclinical hypothyroidism and adverse pregnancy outcomes. Results In 630 cases of pregnant women,there were 8 cases of hyperthyroidism (1.3%),61 cases of hypothyroidism or subclinical hypothyroidism (9.7%),including 5 cases of hypothyroidism (0.8%), 56 cases of subclinical hypothyroidism (8.9%) .There was statistically significant difference in TSH and FT4 between hypothyroidism group and normal control group ( <0.01) .There was statistically significant difference in TSH,FT4 and TT4 between subclinical hypothyroidism group and normal control group ( <0.05) . In 61 cases of hypothyroidism and subclinical hypothyroidism, 39 patients received levothyroxine therapy,22 received no treatment. The incidence of adverse pregnancy outcomes in the treatment group was significantly lower than the untreated group ( <0.05) . Conclusion Thyroid function testing in 6-20 weeks in early pregnancy has important significance in timely founding gestational thyroid dysfunction, so it should be as routine examination project.

Objective To explore the effect on the neural endocrine and gastro intestinal functions when using Chinese traditional medicine in the treatment after the gyno-pathy operation of the wond's complete cutting off. Methods To compare 86 postoperative cases of gyno-pathy (womb's complete cutting off) being treated partly with Chinese medicine wiht the same 75 cases treated during the same period without Chinese medicine in order to investigate the different effect on the recovery of their neural endocrine and gastric intestinal functions. HZ Results The result shows that the patients in the tested group can recouer quickly while the neural endocrine and the gastro intestinal functions of the opposite group cannot recouer or recover very slowly in a long time. Conclusion Traditional Chinese medicine is rather helpul for the recovery of thd postoperative gyno-pathy patients if it is correctly used .

Objective To investigate the indications, maternal and fetal/neonatal risks and treatments of pregnancy after liver transplantation. Methods The related published literatures were summarized and reviewed.Results Most females can recover a regular menstrual cycle. Hypertension, preeclampsia, bacterial and viral infection resulted from immuno-suppression therapy and increasing rate of caesarian section are the major risks for the mother. Abortion and pre-term delivery, premature rupture of fetal membrane, hypoadrenalism, abnormality, immune deficiency, hepatitis virus B and cytomegalovirus and bacterial infection are the major risks for the fetus/neonate.Conclusion Satisfactory results can be obtained in most pregnancies after liver transplantation. Evaluation before gestation and surveillance during gestation by the related departments are mandatory. A guideline based on large cases is necessary for pregnancy after liver transplantation.

Expression of PRL receptor mRNA in Jurkat D1.1 cell line was confirmed by RT PCR. CD154 expression of Jurkat cells was significantly increased when these cells were incubated with PHA and recombinant human PRL, suggesting that binding of PRL to its receptor promotes CD154 expression which plays a role in immune regulation.

Objective:To investigate the expression of prolactin receptor(PRLr) in human immune system.Methods:Specimens of human immune system were obtained from both central and peripheral immune organs, including thymus(thymoma), bone marrow, lymph node and peripheral blood mononuclear cells. Specific fragments of PRLr mRNA were obtained through RNA isolation and RT-PCR amplification and confirmed by DNA sequencing.Results:PRLr mRNA was detected from the thymoma, bone marrow, lymph node and peripheral blood mononuclear cells. All the PRLr cDNA fragments generated by PCR were as long as the expected length, which was 276 bp. The sequencing result of the cDNA was identical to the sequence of PRLr cDNA in GeneBank.Conclusion:The study confirmed that prolactin receptor mRNA was expressed in human central immune organs such as thymus and bone marrow and peripheral immune organs such as lymph node and peripheral blood mononuclear cells. This phenomenon provided a biologic structural evidence that neuro-endocrine hormone PRL might play a modulating role on immune cells from receptor's point of view.

Allopurinol is a xanthine oxidase inhibitor,it can reduce production and excretion of uric acid and oxygen free radicals generation. Allopurinol has good therapeutic effects on gout and hyperuricemia,and can relief symptoms of prostatitis. In addition, allopurinol has protective effects on ischemical reperfusion injury,inflammation and tumor.