Objective To classify all the plant pollen allergens, each allergen sequence available was compared with protein family database. After that, the appearance and taxonomic distribution of each protein family could be known. When made reference to evolutionary analysis, a regular rule of the distribu-tion of all plant pollen allergens could be concluded. Methods Protein family memberships of each allergen were assigned by comparing the sequences with the Pfam database. Representative members of the most a-bundant pollen allergen families were compared with the Uniprot database using the BLAST server. Acces-sion number of all the interesting homologous could be obtained and all the sequence information could be ac-quired by Batch Entrez. Finally, the evolutionary trees were drawn with the help of MEGA4.0 software. Re-sults One hundred and sixty-eight pollen allergens were classified into 26 protein families. Profilins, pollen _allerg_1 and EF hands constituted the most abundant pollen allergen families. Profilins and EF hands oc-curred in almost all allergenic plant families, whereas allergenic Expansins, FAD_binding proteins, Amb_V allergens and Thaumatins were confined to single taxon. Conclusion It is concluded that the highly con-served sequences of allergens families such as Profilin may be one of the most important reasons of the cross reactivities in allergic diseases. The classification of pollen allergens may be helpful to clinical practice and basic research.

Objective To observe the effect of cupping plus Chinese medicinal fumigation on expectoration in patients with chronic obstructive pulmonary disease (COPD) due to phlegm-heat obstructing the lung. Method Eighty-two patients with COPD due to phlegm-heat obstructing the lung were randomized into a treatment group and a control group, 41 cases in each group. The control group was given regular Western medications, while the treatment group was intervened by cupping plus Chinese medicinal fumigation in addition to the treatment given to the control group, once a day, 5 d as a treatment course. The therapeutic efficacies were observed after 2 treatment courses. Result The clinical effective rate of the treatment group was higher than that of the control group, with a statistical significance (P<0.05). Conclusion Cupping plus Chinese medicinal fumigation can enhance the expectoration in patients with COPD due to phlegm-heat obstructing the lung.

Expression of PRL receptor mRNA in Jurkat D1.1 cell line was confirmed by RT PCR. CD154 expression of Jurkat cells was significantly increased when these cells were incubated with PHA and recombinant human PRL, suggesting that binding of PRL to its receptor promotes CD154 expression which plays a role in immune regulation.

Objective:To investigate the expression of prolactin receptor(PRLr) in human immune system.Methods:Specimens of human immune system were obtained from both central and peripheral immune organs, including thymus(thymoma), bone marrow, lymph node and peripheral blood mononuclear cells. Specific fragments of PRLr mRNA were obtained through RNA isolation and RT-PCR amplification and confirmed by DNA sequencing.Results:PRLr mRNA was detected from the thymoma, bone marrow, lymph node and peripheral blood mononuclear cells. All the PRLr cDNA fragments generated by PCR were as long as the expected length, which was 276 bp. The sequencing result of the cDNA was identical to the sequence of PRLr cDNA in GeneBank.Conclusion:The study confirmed that prolactin receptor mRNA was expressed in human central immune organs such as thymus and bone marrow and peripheral immune organs such as lymph node and peripheral blood mononuclear cells. This phenomenon provided a biologic structural evidence that neuro-endocrine hormone PRL might play a modulating role on immune cells from receptor's point of view.

Objective To investigate the indications, maternal and fetal/neonatal risks and treatments of pregnancy after liver transplantation. Methods The related published literatures were summarized and reviewed.Results Most females can recover a regular menstrual cycle. Hypertension, preeclampsia, bacterial and viral infection resulted from immuno-suppression therapy and increasing rate of caesarian section are the major risks for the mother. Abortion and pre-term delivery, premature rupture of fetal membrane, hypoadrenalism, abnormality, immune deficiency, hepatitis virus B and cytomegalovirus and bacterial infection are the major risks for the fetus/neonate.Conclusion Satisfactory results can be obtained in most pregnancies after liver transplantation. Evaluation before gestation and surveillance during gestation by the related departments are mandatory. A guideline based on large cases is necessary for pregnancy after liver transplantation.

OBJECTIVE:To discuss the development prospect of the hospital pharmacists in clinical trials and to accumulate experience for the development of hospital pharmacy. METHODS: The current strengths,weakness,opportunities and threats of the hospital pharmacists were systematically analyzed and evaluated with the Porter's five strengths and PSET models;the development strategies were formulated by SWOT matrix. RESULTS: There were not only the apparent strengths and weakness but also the opportunities and threats for hospital pharmacists in clinical trials. CONCLUSION: At present,hospital pharmacists should take advantage of the opportunities of clinical trials market,stick to the aim of the professional development,spare no effort in discipline construction and training of professional talents and establish the space for their development and their indispensable position by giving priority to scientific research with service as target.

OBJECTIVE:To prospectively investigate the effects of enalapril in decreasing ACE activity,renal function protection in diabetic nephropathy(DN)patients and prevention of deterioration from DN METHODS:62 DN patients were divided into two groups,31 in each group,the sex,age,disease duration and condition were comparable In treatment group,patients received enalapril 10mg/d in addition to routine anti-hyperglycemic and anti-hypertensive agents,in control group,patients had only routine treatment The observation lasted 18～36 months(mean 26 months) The BP,blood sugar and renal functions were compared between two groups before and after treatment RESULTS:In treatment group,blood sugar,BP and renal functions were significantly improved after treatment of enalapril(P0 05) CONCLUSION:Enalapril can lower ACE activity and protect the renal function,these effects do not depend on it’s anti-hypertension result

Objective To explore the effect on the neural endocrine and gastro intestinal functions when using Chinese traditional medicine in the treatment after the gyno-pathy operation of the wond's complete cutting off. Methods To compare 86 postoperative cases of gyno-pathy (womb's complete cutting off) being treated partly with Chinese medicine wiht the same 75 cases treated during the same period without Chinese medicine in order to investigate the different effect on the recovery of their neural endocrine and gastric intestinal functions. HZ Results The result shows that the patients in the tested group can recouer quickly while the neural endocrine and the gastro intestinal functions of the opposite group cannot recouer or recover very slowly in a long time. Conclusion Traditional Chinese medicine is rather helpul for the recovery of thd postoperative gyno-pathy patients if it is correctly used .

Objective To investigate the effect of Atorvastatin Combined Naoxintong Capsule treats to cerebral infarction and carotid atherosclerosis.Methods 134 patients with cerebral infarction and carotid atherosclerosis were diagnosed by MRI or CT scan.They were randomly divided into two groups.67 cases were given atorvastatin versus aspirin received routine treatment in the control group and 67 cases were used Naoxintong capsule in observation group on the basis of routine treatment in the observation group.The blood lipid,serum high sensitive C-reactive protein (hs-CRP) level,neural function defect score (NIHSS),carotid artery intima-media thickness (IMT) were comperred before and after treatment in two groups for treated with 1 courses (6 months).Results The blood lipid had no significant difference between two groups before and after treatment (P ＜ 0.05).The IMT,NIHSS score of high sensitivity C-reactive protein levels had no significant difference in two groups before and after treatment (P ＜ 0.05).The scores of NIHSS,IMT,high sensitive C-reactive protein level respectively (10.08 ± 1.82) ％,(1.06 ± 0.13)mm,(3.89 ± 1.41) mg/L of observation group were more decreased significantly than the control group (respectively (13.92 ±2.01)％,(1.24 ±0.15)mm,(5.02 ± 1.52) mg/L after treatment.Conclusion Atorvastatin Combined Naoxintong Capsule in treatment of cerebral infarction and carotid artery gruel kind hardening shows good therapeutic effect,it is worth for clinical use.