ObjectiveTo investigate the outcomes of mother a nd baby smaller than 37 gestational age caused by preeclampsia. Method sThe data for 122 preterm birth that was single pregnancy with preecla mpsia in our hospital from January 1993 to December 2002 were collected. The pri nciple of diagnosis and treatment, the outcomes were analyzed retrospectively. T he 122 pregnant women were divided into 3 groups according to their gestational age, more than 28 weeks and smaller than 31 +6 weeks was the first group (28 cases), more than 32 weeks and smaller than 33 +6 weeks was the second grou p (26 cases), more than 34 weeks and smaller than 36 +6 weeks was the thir d group (68 cases). The data were statisted with SAS software.Results The earlier the pregnancy-induced hypertension happened, the earlier the preeclampsia occured, the mean time was 14 03 d, 16 85 d,22 22 d, respect ively, (P0.05), the outcomes of mothers were good. Increasing the gestational age under the cond ition that the occurrene of comptications was not increased, 60 64% pregnant wo men accepted the treatment of improving the maturity of fetal lung. There were n o differences among the three groups at the morbidity of perinatal SGA, intracra nial hemorrhage (P 0.05) .ConclusionWe could improve the outcomes of pret erm birth caused by preeclampsia by enhancing the perinatal care, treating and t erminating pregnancy activey Increasing the gestational age, improving the matur ity of fetal lung would decrease the morbidity of severe RDS and improve the pe rinatal outcome effectively.

AIM:To investigate the effects of tanshinone IIA ( Tan) on cardiac action potential in rats with a-cute cerebral ischemia (ACI).METHODS:ACI was established in rats accordingly .Animals were divided into 3 groups:sham group, ACI group, and ACI with Tan treatment group .The defect of neural function in each group was graded , elec-trocardiogram was measured , monophasic action potential was recorded , and the levels of cardiac troponin I ( cTnI ) and creatine kinase-MB ( CK-MB) were examined by commercially available kits .RESULTS:Compared with ACI group , Tan significantly decreased the scores of the defect of neural function , reduced the incidence and duration of the abnormalities in electrocardiogram , prolonged the effective refractory period , action potential duration at 50%repolarization and action po-tential duration at 90%repolarization, and inhibited the elevation of cTnI and CK-MB levels.CONCLUSION:Tan main-tains a stable heart rhythm in ACI rats , which may be related to its protective effect on ischemic myocardium .

Objective To investigate the pathogenesis of gastroparesis in diabetic rats.Methods SD rats were randomly divided into control group and diabetes group.Ten weeks later,gastroparesis model was established.The apoptosis rate and mitochondrial membrane potential of the gastric smooth muscle cells were detected by flow cytometry.Cytochrome C was detected by immunohistochemistry.Results Compared with rats in control group:(1)Typical symptoms of diabetes mellitus appeared;The rate of gastric residual pigment was significantly higher(P

OBJECTIVE:To discuss the research and clinical application of drug eluting stents,in addition,to explore the effects of drug eluting stents on preventing and curing coronary restenosis. METHODS:The databases of Medline and Chinese CNKI were retrieved using computer from 1993 and 2009,and the literature was selected according to inclusion and exclusion criteria. The rapamycin eluting stent and paclitaxel eluting stent were widely used in preventing and curing in stent restenosis,the problems existed in this process were discussed. RESULTS:Drug eluting stents could prevent in stent restenosis following stent implantation,however,the problems such as poor adherence or stent fracture still occurred in clinic. CONCLUSION:Drug eluting stents exhibit efficiency and safety in preventing restenosis,but there still existed problems need to further explore.

Objective: To observe the effect of the combinative supplementation of Formoterol, Indomethacin and Roxithromycin on cancer cachexia (CC) and to study the mechanism. Methods: Male BALB/c mice bearing colon 26 adenocarcinoma for 9 days were served as models of cancer cachexia. The mice were divided into nine groups. Physiological conditions, body weight and food intake were documented every day. Serum levels of cytokine and nutritional markers were detected 7 days after treatment. Results: Tumor-bearing caused a wasting of non-tumor body weight and gastrocnemius muscle. Serum tumor necrosis factor-α, (TNF-α) and interleukin-6 (IL-6) levels were significantly elevated accompanied with nutrition depletion in tumor-bearing mice. After treatment with combinative supplementation of Formoterol, Indomethacin and Roxithromycin, left gastrocnemius weight and serum glucose were improved and the levels of TNF-α and triglyceride were down-regulated. Conclusion: Formoterol, Indomethacin and Roxithromycin had their own positive effects to CC mice. Drugs combinationin in tumor-bearing mice with cancer cachexia could produce synergistic action.

The KCNQ gene family encodes five Kv7 channel sub-units(Kv7. 1-5) and four of these (Kv7. 2-5) channels are ex-pressed in the central nervous system ( CNS ) . Neuronal Kv7 channels participate in both pre-and post-synaptic modulation of neurotransmissions. Activation of neuronal Kv7 channels inhibits neuronal excitability and limits the release of monoaminergic neu-rotransmitter. Kv7 channel openers provide novel therapeutic op-tions in the treatment of disease states characterized by overactiv-ity of monoaminergic neurons ( e. g. schizophrenia, anxiety and drug abuse) . This review summarizes the studies on expression and functional role of Kv7 channels in monoaminergic neurotrans-mission in the CNS and the potential therapeutic effect of Kv7 channel openers on the disease characterized by overactivity of monoaminergic neurons.

Prostate cancer is the most prevalent male urogenital malignancy.Androgen deprivation therapy is the principal method for initial treatment for the patients,but the majority of them will eventually develop progressive disease,a status called castration-resistant prostate carcinoma.Lots of susceptibility genes,tumor suppressor genes and oncogenes,and their variations rdevant to the occurrence and development of prostate cancer have been revealed by the studies of molecular oncology.These findings on the molecular basis of prostate carcinogenesis will further improve the strategies on prevention,diagnosis and clinical management for prostate carcinoma.

OBJECTIVE To apply D-test for detection of inducible resistance of erythromycin to clindamycin in staphylococci and investigate their resistant rate to erythromycin and clindamycin. METHODS According to the standards of NCCLS to detect inducible resistance of erythromycin to clindamycin in staphylococci . RESULTS Co-resistance to erythromycin and clindamycin accounted for 50% and 22.8% in 77 strains of staphylococci (20 of S.aureus and 57 of coagulase-negative staphylococci). The rate of erythromycin resistance and clindamycin sensitivity accounted for 20% and 59.6%. D-test positive rate was 50% and 61.8% of which were erythromycin resistant and clindamycin sensitive respectively. CONCLUSIONS D-test for detection of inducible resistance of erythromycin to clindamycin in staphylococci should be checked in clinical microbiology laboratory in order to help physicians to select MLSb antimicrobial agents correctly.

OBJECTIVE To evaluate the efficacy,the eradication rates of pathogens and safety of levofloxacin in patients with community-acquired pneumonia(CAP) in comparison with therapy using a combination of cefuroxime plus azithromycin.METHODS Fifty five patients with CAP were randomly divided into two groups: levofloxacin alone and cefuroxime plus azithromycin,and the efficacy,the eradication rates of pathogens and the rates of side effects were observed.RESULTS From 30 patients in the levofloxacin group,25 patients(83.3%) were clinically cured and 3 patients(10.0%) were improved.And from 25 patients in the cefuroxime plus azithromycin group, 20 patients(80.0%) were clinically cured and 3 patients(12.0%) were improved. The eradication rates of pathogens were 85.7% and 88.9%,respectively.And the rates of side effects were 3.3% and 4.0%,respectively.CONCLUSIONS There are no significant differences in the efficacy,the eradication rates of pathogens and safety between 2 groups in treating community-acquired pneumonia.

AIM:To construct human interleukin-18 receptor ?(IL-18R?) and interleukin-18 receptor ?(IL-18R?) eukaryotic expression vectors named pcDNA3.1-zeocin/IL-18R? and pcDNA3.0/IL-18R? respectively,and then stably transfect pcDNA3.0/IL-18R? into 293 cells in order to establish the cell line stably expressing IL-18R? and cell model which responds IL-18 signal transduction in vitro. METHODS:The experiment was performed at the laboratory of Department of Immunology,Peking University Health Science Center from June 2006 to May 2007. DNA encoding IL-18R? or IL-18R? was amplified and cloned into pcDNA3.1-zeocin or pcDNA3.0 vector,respectively. The recombinant of pcDNA3.0/IL-18R? was transfectd into 293 cells. After screening culture by G418,stable transfected cell line was established and the expression of IL-18R? was identified by Western-blot assay. The stable cell line with the combinant eukaryotic expression vector pcDNA3.1-zeocin /IL-18R? to establish the IL-18 signaling pathway,which was detected by NF-?B-dependent Luciferase. RESULTS:The eukaryotic expression vectors of IL-18R? and IL-18R? were constructed successfully. The gene of IL-18R? stably expressed in 293 cells and cell model of IL-18 signaling transduction in vitro could be established in these stable 293 cell lines. CONCLUSION:The establishment of NF-?B-activated cell model in vitro which responds to IL-18 can provide solid foundation for further experimental studies on IL-18 signaling transduction pathway.