ObjectiveTo investigate the outcomes of mother a nd baby smaller than 37 gestational age caused by preeclampsia. Method sThe data for 122 preterm birth that was single pregnancy with preecla mpsia in our hospital from January 1993 to December 2002 were collected. The pri nciple of diagnosis and treatment, the outcomes were analyzed retrospectively. T he 122 pregnant women were divided into 3 groups according to their gestational age, more than 28 weeks and smaller than 31 +6 weeks was the first group (28 cases), more than 32 weeks and smaller than 33 +6 weeks was the second grou p (26 cases), more than 34 weeks and smaller than 36 +6 weeks was the thir d group (68 cases). The data were statisted with SAS software.Results The earlier the pregnancy-induced hypertension happened, the earlier the preeclampsia occured, the mean time was 14 03 d, 16 85 d,22 22 d, respect ively, (P0.05), the outcomes of mothers were good. Increasing the gestational age under the cond ition that the occurrene of comptications was not increased, 60 64% pregnant wo men accepted the treatment of improving the maturity of fetal lung. There were n o differences among the three groups at the morbidity of perinatal SGA, intracra nial hemorrhage (P 0.05) .ConclusionWe could improve the outcomes of pret erm birth caused by preeclampsia by enhancing the perinatal care, treating and t erminating pregnancy activey Increasing the gestational age, improving the matur ity of fetal lung would decrease the morbidity of severe RDS and improve the pe rinatal outcome effectively.

AIM:To investigate the effects of tanshinone IIA ( Tan) on cardiac action potential in rats with a-cute cerebral ischemia (ACI).METHODS:ACI was established in rats accordingly .Animals were divided into 3 groups:sham group, ACI group, and ACI with Tan treatment group .The defect of neural function in each group was graded , elec-trocardiogram was measured , monophasic action potential was recorded , and the levels of cardiac troponin I ( cTnI ) and creatine kinase-MB ( CK-MB) were examined by commercially available kits .RESULTS:Compared with ACI group , Tan significantly decreased the scores of the defect of neural function , reduced the incidence and duration of the abnormalities in electrocardiogram , prolonged the effective refractory period , action potential duration at 50%repolarization and action po-tential duration at 90%repolarization, and inhibited the elevation of cTnI and CK-MB levels.CONCLUSION:Tan main-tains a stable heart rhythm in ACI rats , which may be related to its protective effect on ischemic myocardium .

Emergency gastroscopy was performed for 146 patients with upper gastrointestinal hemorrhage admitted to Tieying Hospital, Beijing to identify causes of bleeding and establish diagnosis. One hundred and thirty-nine of them were cured with endoscopic treatment, but two failed so to change to surgical operation. It suggested that early gastroscopy is a convenient, easy and safe method in diagnosis and treatment of upper gastrointestinal hemorrhage.

Fetal origins of adult disease,the thrifty phenotype hypothesis, nutritional programming,metabolic programming indicate that nutrition in early life is important.Epidemiological survey demonstrates that insulin resistance is relataed to intrauterine growth retardation,but the exact mechanism is indefinite.This article is about the influence of intrauterine growth retardation to the structure and function of pancreas,and the genes that related to insulin resistance.

Microorganism distributes in the organs of human body which connect with external environment, especially those organs in the gastrointestinal tracts, and it also plays a fundamental role in the physiopathology of the host's body. Because the microorganism is very small and has a great variety, it is difficult to reveal the significance of microorganism in the human physiopathology comprehensively and deeply. With the development of molecular biology, genomics, bioinformatics and other disciplines, the microbiome research will be more possible and easier. There are two key contents of microecology. One of these is to identify and quantify the diversity of microorganism, and the other is to reveal activity and the physiopathological function of microorganism in the host. Microbiome research methods, therefore, can be summarized as the traditional detection methods, construction of gene library, the genetic fingerprint analysis and molecular hybridization techniques and so on.
Computational Biology ; Gastrointestinal Microbiome ; Gastrointestinal Tract ; microbiology ; Humans

Objective To study far femoral malignant bone tumor salvage limb treatment with allogeneic heterotopic half-joint transplantation and the use of GSH nail Methods Eleven cases with far femoral malignant bone tumor were administered tumor resection,transplanted with allogeneic heterotoeic bone half-joint,and fixed with GSH nail Results Nine out of the 11 cases were followed up.None of them had infection,rejection or heterotopic bone fracture.All of them can move with walking after 6 months.The effects of surgery was rated as excellent for 3 cases,fine for 4 cases andbad for 2 cases according to Mankin's rating criteria.Conclusion Salvage limb treatment for bone tumor can improve patients' quality of life.Allogeneic heterotopic bone,with rich source and acceptable joint surface,is a good graft for the treatment of bone defect after bone tumor resection.

Mefformin is the first-line oral medicine used to treat type 2 diabetes.Besides its glucose-lowering effect,Mefformin can inhibit the proliferation of cancer cells,especially colorectal cancer cells.In recent years,there is mounting evidence that mefformin could reduce the risk and mortality of colorectal cancer, and its mechanism includes activating AMP activated protein kinase pathway,interfering with cell cycle,redu-cing insulin resistance,inhibiting the inflammatory response and killing colorectal cancer stem cells,etc.

As one important member of the biological sample library , blood samples are rich in biological molecules, which can be used for disease diagnosis , prognosis of disease staging , and prognosis et al.compared with tissue samples, blood samples have some advantages , such as easier to access, sampling in a row and rich test items and so on.However, after blood samples were collected, which need to be processed, packed and frozen in ice for long-term preservation container.Otherwise RNA in the blood sample is unstable and easily degradation in normal temperature transportation and preservation condition , thus subsequently affecting the molecular diagnostic biomarkers ′detection, analysis, research and development.In this paper, This review summarizes the current clinical and research work of routine blood collection and preservation methods effecting on blood RNA degradation and solution for avoiding these degrading which can help testing changings of blood biomarker more accurately .

Objective To study the expressions of MCM5 and Ki67 in cervical intraepithelial neoplasia (CIN) and cervical cancer and their clinical significance. Methods Immunohistochemistry (S-P method) was used to detect the expressions of MCM5 and Ki67 in 50 CIN and 49 cervical cancer. Results The positive expression rate of MCM5 and Ki67 in normal cervical epithelium, CIN Ⅰ-Ⅱ, CIN Ⅲ and cervical cancer were 26.7%, 72.0%, 80.0%, 97.9% and 10.0%, 32.4%, 84.0%, 97.9% respectively. The positive expression rate of MCM5 was significantly different between CIN and cervical cancer (P