ObjectiveTo study the mastery, acquiring ways and need of studying the knowl-edge and skill of emergency treatment in non- medical junior college and technical secondary school students. MethodsThe survey was carried out in 600 non-medical junior college and technical sec-ondary school students to investigate their mastery and need of emergency knowledge, the results underwent analysis. ResultsThe mastery of the emergency knowledge was uneven in non- medical junior college and technical secondary school students. 100.0% of them can call 120 correcdy, 58.5% of them mastered the e-mergency treatment of carbon monoxide poisoning, and 25.0% mastered cardiac pulmonary resuscitation. All the students desired to hold the knowledge and skill of emergency treatment. Television, broadcast and internet were the main ways for them to master emergency knowledge. ConclusionsThe mastery of the knowledge and skill of emergency treatment is not good. It is urgent to educate and train them with the knowledge and skill of emergency treatment, and to improve knowledge and skill of emergency treatment.

Objective To summarize the nursing experience in prehospital first-aid of patients with cardiac traumas in order to improve the cooperation capability of prehospital first-aid. Methods The nursing cooperation of prehospital first-aid of 33 patients with cardiac trauma was analyzed retrospec-tively. Results 24 cases of them were rescued successfully, and were completely recovered without se-quelas. 4 cases complicated with severe multiple trauma, died after arriving at hospital, and 3 cases com-plicated with multiple trauma died of complications, while 2 cases with injury of thoracic aorta died during the surgery. Conclusions Early emergency treatment and judgment of traumatic condition could pro-vide valuable chance for inhospital first-aid.

Objective To study the relationship between the expression of chemokines monocyte chemoattractant protein 1 (MCP 1) and the biological behavior of colorectal carcinoma by detecting chemokine mRNA and protein in human colorectal cancerous tissues. Methods The expression of the MCP 1 mRNA was detected in colorectal carcinoma collected freshly from surgical specimens by RT PCR and the expression of the MCP 1 protein was assessed in colorectal carcinoma tissues collected from surgical specimens by immunohistochemistry. Results All the 12 colorectal carcinoma expressed the MCP 1 mRNA, which was detected by RT PCR; MCP 1 protein was detected in 90% of the tumors; The expression of the MCP 1 protein in colorectal carcinoma was correlated with its state of metastasis and the Dukes' stage. Conclusions The expression of chemokine MCP 1 in colorectal carcinoma may influence its biological behaviour.

Objective To explore the repair effect of methylcobalamin treatment of nerve after spinal cord injury.Methods 108 cases of patients with acute spinal cord injury were selected treated in our hospital from 2010 April to 2014 April , including 60 cases of male, female 48 cases, were randomly divided into Mecobalamin group(n=56) and control group(n=52), mecobalamin group was treat with a cobalt amine, control group was given conventional treatment (dexamethasone intravenous drip note).According to American spinal cord injury score (ASIA) to set sensory function, motor function and spinal cord function score criteria, observed and record ed the sensory function, motor function, recovery of neurological function and complications the two groups of patients were treated for 6 weeks and followed up for 6 months.Results Spinal cord injury patients after 6 weeks and 6 months follow-up, the improvement of sensory and motor function of mecobalamin group were significantly better than the control group ( P <0.01 );Mecobalamin group patients after first to 3 weeks of recovery of neurological function was higher than those of the control group (P<0.05);the 2 groups were found urinary tract infections, lung infections, vomiting, reaction of ulcer complications, but the difference was not statistically significant ( P>0.05).Conclusion In patients with acute spinal cord injury treated by mecobalamin treatment can improve the nerve function damage condition, improve the prognosis and quality of life.

Objective To explore the mechanism of macro-reentry atrial tachycardia and to guide catheter ablation using electroanatomic mapping system in 3 patients.Methods Three patients(two females),aged 51?12 years,with atrial tachycardia were included.The mean history of symptom was 19?11 years.Conventional electrophysiological study was performed to determine the location of atrial tachycardia before the three dimensional geometry reconstruction.After voltage and activation maps were constructed,the mechanism of tachycardia was analyzed and the slow conduction areas(critical isthmus) were verified.Radiofrequency energy was delivered using irrigated-tip catheter.Results Though there was no evidence to suggest structural heart diseases,scar areas were found in the mapped atria in all the three cases.The mechanism of atrial tachycardia was found to be counter-clockwise macro-reentry around tricuspid valve,counter-clockwise macro-reentry around superior vena cava,and figure "8" reentry in left atria in the 3 patients respectively.The respective critical isthmus was found to be between the lateral scar and tricuspid valve,the lateral scar and superior vena cava,and two scars on the left atria roof.Ablation in the critical isthmus terminated all tachycardia.There were no atrial tachycardia recurrence during a follow-up of 9-10 months.Conclusion The substrate and electrophysiological mechanism of macro-reentry atrial tachycardia could be identified clearly,and navigation ablation could be performed effectively under the guidence of electroanatomic mapping.

Objective To study some sensitive electrophysiological parameters in surveillance of allograft rejection.Methods Forty rats underwent heterotopic heart transplantations.IMEG was re- corded by an epicardiac unipolar pacing lead fixed at the right ventricular outflow tract.QRS amplitude and heart rate were determed daily in 10 syngeneic and 30 allogeneic transplants.Syngeneic transplants were killed at 7 th postoperative day,and allogeneic transplants killed at 3 rd,5 th and 7 th postopera- tive day.Histopathologie studies were performed at every transplanted heart.Results In syngeneic group,QRS amplitude kept constant after the transplantation while no significant differences were ob- served at the 3 rd,5 th and 7 th postoperative day.QRS amplitude was dropped obviously in allogeneic group after the first two postoperative days whereas significant differences were observed at the rejec- ting and non-rejecting hearts.Conclusions IMEG is a valid method to monitor acute allograft rejec- tion.QRS amplitude is a more sensitive electrophysiological parameter to diagnose severe rejections than heart rate,while mild rejections were not detected by this method.

Objective To evaluate the effect of vascular endothelial growth factor (VEGF) expression on varicose recurrence in esophagus after devascularization in portal hypertensive rats. Methods Murine pre-hepatic portal hypertension (PHPH) was induced by partial portal vein stenosis. 3 weeks after the establishment of the PHPH, all rats including sham operation group (SO) underwent devascularization. Esophagus was sampled at the time of 1, 3, 5, 7, 14, 30, 90 days after devascularization respectively. The expression of VEGF and factor Ⅷ related antigen (FⅧAg) was detected by immunohistochemistry,and the microvessel density (MVD) was counted under FⅧAg staining. ResultsCompared to the SO rats, the MVD of esophagus in PHPH rats increased significantly before devascularization(P0.05). At the third month after devascularization, the MVD of PHPH rats was higher than that of SO rats. In SO rats, MVD increased after devascularization, reaching the peak on the third day and then levelling off.The expression of VEGF before devascularization in PHPH rats was significantly higher than in SO rats, and VEGF experssion was on the pinnacle in two groups after devascularization, but the expression time of PHPH rats was significantly longer than SO rats(P

OBJECTIVE:To study the effect of ligustrazine injection(LTZ)on DNA synthesis of vascular endothelial cell.METHODS:In vitro cultured cell line of human umbilical vein endothelial cells,ECV304,were adopted to measure the ef?fects of LTZ on DNA synthesis by 3 H-TdR incorporation.RESULTS:LTZ could inhibit DNA synthesis of ECV304in a dose-dependent manner.CONCLUSION:The mechanism of anti-angiogenesis of LTS might be associated with inhibiting DNA synthesis of vascular endothelial cell.

Objective The self-microemulsifying drug delivery system(SMEDDS) with tanshinone ⅡA was prepared in order to develop its new dosage forms.Methods Pseudo ternary phase diagrams were used to evaluate the self-microemulsification existence area under emulsifier,coemulsifer,and oil phase.The HPLC analysis in vitro was set up.Solubility in various vehicles was determined.The self-microemulsification efficiency was assessed,such as stability,particle size,and Zeta potential.Results The solubi-lity of tanshinone ⅡA in SMEDDS was about 2.5 mg/g,droplet size was within 20 nm,and the absolute value of Zeta potential was over 60 mV.The stability of SMEDDS with tanshinone ⅡA was better in centrifugal condition with high temperature but not good enough to the light.Conclusion The SMEDDS can make tanshinone ⅡA solublized in water,and is an optimum vehicle in new dosage forms of tanshinone ⅡA.

Objective To discuss the correlation of the p53 overexpression and human papilloma virus (HPV) infection with the occurrence, development of the vulvar squamous cell carcinoma in elderly women. Methods Samples from 68 patients with vulvar squamous cell carcinoma and samples of formalin-fixed paraffin-embedded blocks from 38 patients with vulvar dystrophy were determined by immunohistochemistry. HPV infection was examined with monoclonal or polyclonal antibodies. Fifteen normal vulvar skin samples were used as controls. Results Positive expression rate of p53 and HPV in vulvar squamous cell carcinoma samples were 54.4% and 45.6% respectively, in the dystrophy samples were 18.4% and 55.3% respectively. All showed significant difference between the two groups. None was positive in the controls. Conclusions There are high expression of p53 and HPV in the elderly women with vulvar squamous cell carcinoma. p53 mutation and high risk HPV infection play an important role in the development of vulvar squamous cell carcinoma.