Objective To explore the clinical application and value of MIPO technique in treating distal tibial fracture.Methods During February 2007 and January 2009,27 patients with distal tibial fractures were treated by MIPO technique using LCP.Based on the AO fracture classification system,7 patients were classified into A2,9 into A3,7 into B2 and 4 into C1.The mean age was 43 years old ranging from 26 to 72 years old.Results The mean follow-up duration was 12 months ( between 4 and 21 months ).According to the Johner-Wruhs score classification system,all the patients were well treated,with excellent efficacy in 19 patients,good in 7 patients and acceptable in 1 patient.No infection or skin putrescence occurred.Conclusion MIPO technique is an ideal measure to treat distal tibial fracture with the advantages such as less incision,higher healing rate,nicer appearance,more stable fixation,less tissue infection,earlier functional exercise and weightbearing activity.

Objective To study the indications of carotid endarterectomy(CEA) and perioperative managements . Methods The clinical data of 11 patients with transient ischemia attack (TIA) and carotid artery stenosis who underwent CEA were reviewed retrospectively. Results After CEA, the TIA symptoms disappeared in all patients, and the chronic symptoms of cerebral ischemia had obvious improvement in 4 cases . There were no serious postoperative complications, such as cerebral hemorrhage, hemiplegia, etc. Conclusions CEA may be considered when one or both sides of carotid stenosis more than 50% is discovered by Duplex scanning, digital subtraction angiography or MRA in patients with TIA. Stage operation for bilateral carotid occlusive lesion is safer. Paying attention to perioperative managements is important for reducing operative complications.

Objective:To evaluate the efficacy and security of two type of rotary nickel titanium system (Twisted File and ProTaper Universal)for root canal preparation based on micro-computed tomography (micro-CT).Methods:Twenty extracted molars (including 62 canals)were divided into two experimen-tal groups and were respectively instrumented using Twisted File rotary nickel titanium system (TF)and ProTaper Universal rotary nickel titanium system (PU)to #25/0.08 following recommended protocol. Time for root canal instrumentation (accumulation of time for every single file)was recorded.The 0-3 mm root surface from apex was observed under an optical stereomicroscope at 25 ×magnification.The presence of crack line was noted.The root canals were scanned with micro-CT before and after root canal preparation.Three-dimensional shape images of canals were reconstructed,calculated and evaluated. The amount of canal central transportation of the two groups was calculated and compared.Results:The shorter preparation time [(0.53 ±0.1 4)min]was observed in TF group,while the preparation time of PU group was (2.06 ±0.39)min (P<0.05).In mid-root level,TF group shaping resulted in less ca-nal center transportation than PU group [(0.070 ±0.056)mm vs.(0.097 ±0.084)mm,P<0.05]. No instrument separation was observed in both the groups.Cracks were not found in both the groups ei-ther based in micro-CT images or observation under an optical stereomicroscope at 25 × magnification. Conclusion:Compared with ProTaper Universal,Twisted File took less time in root canal preparation and exhibited better shaping ability,and less canal transportation.

Objective: To compare the incidences of root cracks after canal instrumentation with HyFlex CM system and the ProTaper Universal system .Methods:Sixty mandibular incisors were mounted in resin blocks with simulated periodontal ligaments , and the apex was exposed .The control group of 20 teeth was not prepared , and the other 40 teeth were divided into 2 experimental groups ( n=20 ) .The 40 root canals of the experimental groups were instrumented using HyFlex CM and ProTaper Universal to the major apical foramen ( AF).The horizontal sections 1 mm, 2 mm, and 3 mm from the apex were observed under an optical stereomicroscope at 25 ×magnification .The presence of cracks was noted . Results:No cracks were found in the control teeth .Cracks were found in 1 of 20 ( 5%) teeth in HyFlex CM group , and 17 of 20 ( 85%) teeth in ProTaper Universal group .The difference between the two experimental groups was statistically significant (P<0.01).Conclusion: The HyFlex CM files caused fewer root cracks than the ProTaper Universal files during the root canal instrumentation .

Objective To determine the hair selenium level by material microwave digestion-atomic fluorescence spectrometry. Methods The selenium in hair was determined by hydride atomic fluorescence spectrometry after microwave digestion, and the method was improved by exploring the test conditions. The linear relationship and linear range of the standard curve, the detection limit, the precision and the accuracy of the sample determination were implemented under the optimized conditions. The selenium value of standard material of human hair was tested by material microwave digestion-atomic fluorescence spectrometry. Results The selenium detection limit of material microwave digestion-atomic fluorescence spectrometry was 0.095 μg/g, the correlation coefficient of standard curve was 0.9997, the relative standard deviation was 1.32% - 2.48%, the average recovery rate was 93.4% - 98.2%, and the selenium value of standard material of human hair was in the standard value range of uncertainty, which met the requirements. Conclusion The method is simple, accurate and precise, and it is an accurate and reliable method for determination of selenium in hair.

Objective To clone and analyze the coding region of methyltransferase gene from four species of Huperziaceae, the species included Huperzia serrata var.longipetiolata, Phlegariurus minchegensis, Phlegariurus austrosinicus and Phlegariurus petiolatus.Methods The methyltransferase coding region sequences were cloned by RT-PCR with the template of total RNA extracted from four Huperziaceae.Then the sequences were analyzed by means of BLAST and MEGA 5.0 software.ResuIts The coding region sequences of the four species of Huperziaceae were highly similar to the methyltransferase in the database.ConcIusion The methyltransferase genes of the four species of Huperziaceae were cloned in this study.The result will provide a foundation for further elucidate the mechanism of Huperzine A biosynthesis and metabolic pathways in Huperziaceae plants.

Objective To evaluate the effect of azithromycin on class Ⅰ integron-integrase gene (intI 1 )mRNA expression in biofilm-forming (BF)Pseudomonas aeruginosa (PA).Methods intI 1 of 10 PA strains isolated from a hospital were detected,1 strain with positive BF+intI 1 was selected for culture,blank control group and three az-ithromycin trial groups (divided according to 3 concentrations:16 mg/L,32 mg/L,and 64 mg/L)were set,experi-ments were repeated 5 times,expression of intI 1 mRNA were detected by RT-PCR.Results Relative expression of intI 1 mRNA in azithromycin groups of 16 mg/L,32 mg/L,64 mg/L,and control group were (1 .15 ±0.04), (12.47±3.10),(19.71 ±0.78 ),and (1 .00 ±0.00),respectively,there were significant difference among four groups(F =163.82,P <0.001 );intI 1 mRNA expression between 16mg/L azithromycin group and control group was not significantly different (P >0.05),but among other groups were significantly different (P <0.05 ),intI 1 mRNA expression in azithromycin groups increased with the enhancing concentration of azithromycin in culture so-lution .Conclusion Expression of intI 1 gene mRNA in BF PA can be up-regulated by the present of azithromycin, which may improve the probability of drug-resistant genes,and promote drug-resistant gene recombination.

Libraries are faced with the crisis of being marginalized in the era of network.Medical academic library should thus adapt to the changing information behaviors of users in study , teaching and scientific research and pro-vide their service according to the user needs-orientedsubject librarian service model , mobile library service model, and sharing commons service model.The service provided in the top 18 medical academic libraries in China according to the 3 models was analyzed, which showed their low popularization rate, insufficient depth and repeti-tion.Measures that should be taken for the solution of such problems were put forward.

Objective To study the nursing of totally endoscopic coronary artery bypass grafting (TECAB)using da Vinci robot.Methods 60 patients who accepted TECAB from January 2007 to December 2010 were selected.All patients underwent coronary arteriography,which showed severe stenosis in left anterior descending artery(LAD).8 of them complicated with right coronary artery stenosis or left circumflex coronary (LCX)stenosis.All patients were given education and nursing care from admission to discharge.Results After operation,angina pectoris alleviated in all cases,no surgery-related complications occurred.The bridge vessel was all cleared.All patients were discharged after rehabilitation.Conclusions Totally robotic TECAB is a new minimally-invasive surgery.Perioperative education is important.In early postoperative course,nurses should pay attention to bleeding condition of drainage and pulmonary nursing.Early postoperative exercise is a must,which can promote the recovery of all systems.

Objective To investigate the nursing intervention on the effect of diagnosis and treatment of emergency patients with urolithiasis. Methods 98 patients with urolithiasis in emergency department of our hospital from January 2009 to January 2011 were randomly assigned into the observation group and the control group with 49 cases in each group. The control group received routine care, on this basis the observation group was given systemic nursing intervention. The tolerance, SAS score and satisfaction degree was compared. Results 40 patients in the observation group was well tolerated, accounting for 81.6%, significantly higher than 63.3% of the control group. SAS score and satisfaction degree of the observation group were significantly better than the control group. Conclusions Nursing intervention for emergency patients with urolithiasis can significantly reduce pain, improve the mental state and increase their satisfaction degree, which is worthy of clinical application.