Manganese-enhanced MRI (MEMRI) optic nerve tracing in vivo is a technology which can reflect the axonal transport status and visual pathway connection by utilizing divalent manganese ion's transportability in axons and its characteristics of paramagnetism,and such potential optic nerve tract-tracing technique has been widely used in ophthalmologic research.Mn2+ enters the neurons through the voltage-gating Ca2+ channel and is transported by microtubule system which is not depended on cellular electric activity.However,the trans-synaptic transport of Mn2+ relies on membrane potential.Taking advantages of MEMRI,optic nerve injury-related research could be implemented.Mn2+ is an intracellular contrast agent with metal toxicity,so low dose,fractionated,targeted delivery of Mn2+ is recommended to alleviate the adverse effects of Mn2+.Herein,the mechanism of divalent manganese ion optic nerve tracing,application of MEMRI in ophthalmologic research field,drug delivery approach of divalent Mn2+ and its toxicity were summarized.

In the past few years, people have a new understanding of the mechanism of Epigenetic disease. Epigenetic modification processes involved in human disease include DNA methylation, histone acetylation and methylation, RNA-associated silencing.Epigenetic modification related with cell growth, differentiation, apoptosis, transformation and tumor progression-related gene transcription. There has been a long time that the gene mutations involved in tumor formation. In recent years.more and more evidences that genetic and epigenetic modifications leading to the formation of tumors. Epigenetic modifications has also play a very important role in the formation of tumors. This article discussed the DNA methylation.histone post-translational modification anomalies and small interference RNA impact on the formation of leukemia. It provides new prospects for the treatment of leukemia.

Objective To investigate the effect of Tetramethyl Pyrazine(TMP) for treating cerebral infarct by observing infarct areas,and to research its function mechanism by observing the changes of the expressions of ED1,TNF-? and IL-1? in the infarction region after focal cerebral ischemia reperfusion injury.Methods Sixty SD male rats in healthy condition were randomly and averagely divided into normal group,model group and TMP high,middle and low dose group.The cerebral infarct animal model was reproduced by modified thread-tie method.TMP high dose group was administered TMP 140 mg/kg,middle dose group was 120 mg/kg and low dose group was 100 mg/kg by intravenous.Then all the rats were killed.Thirty rats were taken to evaluate whether TMP can decrease the ratio of cerebral infarction areas in the TTC-reacting and formalin fixation brain tissue slices taken from the above-mentioned killed rats,with Pathology Image Analysis System.The masculine of ED1,IL-1? and TNF-? in the immuno-reacting cells in cerebral infarction brain tissue slice taken from other thirty killed rats were observed.Results TMP groups decreased the ratio of cerebral infarction areas,and also decreased ED1,TNF-? and IL-1? in immuno-reacting cell.Conclusion TMP can decrease the formation of cerebral infarction caused by cerebral ischemia reperfusion and its effect is possibly related to the activation of microglia,as well as the immuno-positive expressions of TNF-? and IL-1?.

Objective: To explore auxiliary diagnostic value of fragmented QRS complex on ECG for acute myocardial infarction (AMI).Methods: Clinical data of 102 AMI patients (AMI group) and 132 CHD patients (CHD) group, who were treated in our hospital from Apr 2013 to Apr 2014, were retrospectively studied, another 120 healthy subjects undergoing physical examination in our hospital during the same period were regarded as normal control group.AMI was diagnosed by myocardial perfusion tomography, all subjects received ECG examination, and incidence rates of fragmented QRS complex and pathological Q wave at different sites were recorded.Sensitivity, specificity and accuracy of fragmented QRS complex and pathological Q wave diagnosing AMI were compared.Results: Incidence rate of fragmented QRS complex was highest on inferior wall in AMI group, and that of pathological Q wave was highest on anterior wall in AMI group.Except fragmented QRS complex in sidewall, compared with normal control group, there were significant rise in incidence rates of fragmented QRS complex on anterior wall (0.8% vs.22.5%, 13.6%) and inferior wall (6.7% vs.26.5%, 17.4%);pathological Q wave on anterior wall (0 vs.41.2%, 29.5%), inferior wall (0.8% vs.40.2%, 35.6%) and side wall (0 vs.2%, 2.3%) in AMI group and CHD group, P<0.01 all.Compared with pathological Q wave, there was significant rise in diagnosing sensitivity for AMI (40.00% vs.89.47%), for CHD (41.94% vs.91.50%) of fragmented QRS complex (P<0.01 both), but there were no significant difference in specificity and accuracy between them (P>0.05 all).Conclusion: Fragmented QRS complex can be used as an important auxiliary index diagnosing acute myocardial infarction.When combined with pathological Q wave, it can further improve diagnostic value of ECG for acute myocardial infarction.

mia and hypertension are the major risk factors for occurrence of diabetic retinopathy.High UA may be also associated with occurrence of diabetic retinopathy.

SWOT method was used to analyze the internal strengths,weaknesses,external opportunities and threats of Nanjing General Hospital of Nanjing Military Command,PLA. Based on the results,four development policy were put forward:developing the sections prepared for war; inducing invigorative system in human resource management; enhancing the value of nosocomial brand; strengthening of the audit about the medical cost accounting.

Objective To explore the effect of Kangfu antiphlogistic suppository combined with ceftriaxone on the treatment of chronic pelvic inflammation.MethodsFrom March 2015 to January 2017 in Deqing hospital of traditional Chinese medicine, 80 cases with chronic pelvic inflammatory as research object were divided into the observation group and the control group, 40 cases in each group.The control group were given Ceftriaxone, at this basis, the observation group were given Kangfu antiphlogistic suppository, The effect and the adverse reaction in the two groups were compared.ResultsThe effect was 92.5% in the observation group, which was higher than that in the control group 77.5%, the difference was statistically significant,P<0.05.There was no adverse reaction in the 2 groups.ConclusionThe effect is exactly that Kangfu antiphlogistic suppository combined with ceftriaxone sodium on the treatment of chronic pelvic inflammation, which is worthy of clinical use.

Aim To investigate the effect of Sonic Hedgehog on normal hearts and hypoxic-ischemic myo-cardial cells. Methods A method for left anterior de-scending artery ( LAD ) ligation was employed to con-struct the myocardial infarction model, and ultrasonic cardiogram was used for identification. Western blot and immunofluorescence staining were used to detect expressions of Shh, Ptch-1, Smo and Gli-1 in H9C2 cells and H2 O2-induced H9C2 cells, and that in 12 ca-ses of myocardial infarction tissues and 9 cases of nor-mal myocardium, respectively. Agonist and antagonist of Shh pathway were adminstered in the hypoxic-ische-mic myocardial H2 O2-induced H9C2 cell model, and once again expressions and strength of Shh, Ptch-1, Smo, Gli-1 were detected. Results Shh and Gli-1 were not expressed in normal hearts, but expressed in hearts with myocardial infarction;Ptch-1 and Smo were expressed in both normal hearts and hearts with myo-cardial infarction. Under the action of agonist, expres-sions of Shh and Gli-1 increased in the hypoxic-ische-mic H9C2 cell model. Similarly, Shh and Gli-1 were not expressed in normal H9C2 cells, but in H2 O2-in-duced H9C2 cells, and Ptch-1 and Smo were expressed in both normal H9C2 and in H2 O2-induced H9C2 cells. Conclusion Shh signaling pathway can be acti-vated in the condition of ischemia and oxidative stress, and then it promotes the repairing of myocardial cell damage.

[Objective] To evaluate and describe CT findings in patients with pathologically confirmed retroperitoneal fibrosis. [Methods] CT findings in 8 patients (6 males, 2 females) pathologically confirmed retroperitoneal fibrosis were retrospectively reviewed and pertinent literature was discussed. [Results] Retroperitoneal fibrosis showed similar features in CT imaging: retroperitoneal irregular mass around abdominal aorta embedding adjacent tissue, with mild or moderate enhancement, unilateral or bibateral ureterectasis with hydronephrosis. [Conclusions] The pristine clinical manifestation of retroperitoneal fibrosis is occult and nonspecific. CT examination is a valuable approach to diagnose retroperitoneal fibrosis.

[Objective] To evaluate the effect of Tiepi Fengdou Granule(TFG) as adjuvant treatment in cancer patients during chemotherapy.[Methods] Twenty patients were treated with TFG,and then we added up integral calculus of Yin Deficiency symptoms,tested blood routine,hepatic and renal function and subgroup of T-cell and NK cell.[Results] The Yin Deficiency symptoms of 7 cases(35%) were improved significantly,and 13 cases were partly improved(65%).Each integral calculus of Yin Deficiency symptoms was descended.The tested blood routine,hepatic function and subgroup of T-cell and NK cell were changed significantly.[Conclusions] TFG could control the symptoms of Yin Deficiency and improve immune function for cancer chemotherapy patients.