Of all types of intracranial hemorrhage (ICH) in the neonates, germinal matrix intraventricular hemorrhage(GM-IVH) in the premature infant is the most common type, mainly attributed to the existence of immature germinal matrix, lt's usually lack of visible and specific symptoms and signs in the nervous system, so its early and final diagnosis depends on the imaging examine. GM-IVH can cause periventricular hemorrhagic infarction, post-hemorrhagic hydrocephalus, periventricular leukomalacia and the associated cerebellar hemorrhagic injury, which are critical determinants of neonatal morbidity, mortality, and neurodevelopmental outcome in the survivors. The overall aim of this article is to review the current knowledge of the cause,mechanisms, imaging diagnosis, complication, management and outcome of GM-IVH in the preterm infant.

Many studies have demonstrated that blockade of angiogenesis by antiangiogenic drugs in di-fferent ways can control the growth of tumor.Antiangiogenic agents can target different sites:vascular growth pro-moting f_ators,endothelial cells,basement membrane degradation,epidermal growth factor receptor,ete.Antian-giogenic agents have promising prospect.

Radiotherapy is important in cancer treatment, but improving the therapeutic effect of irradiation and decreasing its toxicity to normal human tissues is still a global problem. Epidermal growth factor receptor (EGFR) is a member of ErbB family and is an important transmembrane receptor with signal-transduction tyrosine kinase activity. EGFR can direct cellular migration, adhesion, proliferation, differentiation, and apoptosis, and plays a fundamental role in the development and growth of many types of human tumor cells. A series of preclinical studies showed that EGFR inhibitors can enhance the antitumor activity of ionizing radiation. EGFR inhibi-tors regulate radio-sensitization through multiple mechanisms, including cell cycle alterations, DNA repair modulation, and anti-angio-genesis. Reasonable application of EGFR inhibitors will effectively increase the radio-therapeutic effect, extend the local control of tu-mor, and improve a patient's quality of life.

Objective To analyze the effect of CO2 laser under suspension laryngoscope on patients with vocal cord polyp. Method 118 patients with vocal cord polyp from November 2013 to August 2015 in our hospital were chose as research subjects, all patients were divided into observation group (n=54) and control group (n=64) accord﹣ing to different treatment. Control group: patients received conventional laryngoscope resection; observation group:patients received CO2 laser under suspension laryngoscope. Then compare the two group patients' voice indexes, a﹣cute phase proteins, rehabilitation and recovery speed after treatment. Results Observation group patients' F0, HNR values were higher than control group patients, PPQ, APQ, NNE values were lower than that in control group (P<0.05);early observation group patients' postoperative serum CRP, α1-AG, CER,β2-MG values were lower than con﹣trol group patients, ALB value was higher than control group patients (P< 0.05); observation group patients' cure rate was higher than control group patients, valid and invalid rates were lower than control group patients, the first sound time, sound full recovery time, discharge time were shorter than control group patients (P< 0.05). Conclusion Vocal cord polyp patients receive laser under suspension laryngoscope treatment, can effectively promote recovery rate, have advantages of minimally invasive and rapid recovery after surgery.

P300/CBP is one of the most important high molecular weight protein histone acetyltransferase ( HAT) Although it is encoded by multiple different genes , P300/CBP is highly homologous , Because they have the similar amino acid sequence and functions ,and belong to the same class of proteins ,normolly they are all called P300/CBP.P300/CBP is involved in the activation of many kinds of transcription factors ,the protein itself alsohas acetyltransferase activity ,and is capable of acetylation of 4 core histones and transcription factor .More and more studies have confirmed the relationship of P 300/CBP variation withmultiple human diseases , including in-flammation,diabetes,heart disease and especially cancer .In tumor P300/CBP is associated with some pathways , and these pathways play a different roles in the tumor .Although P300/CBP is usually regarded as a tumor sup-pressor factor ,is plays different roles in different tumors ,This review mainly introduces the relationship of P 300/CBP with some solid tumor disease genes ,related transcription factors and their signaling pathways .

Gastrointestinal bleeding is a severe,complicated and commonly seen disease in Department of Digestive Diseases,the frequent etiology is peptic ulcer,acute gastric mucosal lesion,esophageal gastric varices and digestive tract tumors. In recent years,clinicians gradually noticed a kind of disease characterized by acute,recrudescent and life-threatening bleeding,that is the non-variceal vascular originated gastrointestinal hemorrhage. The guidelines and scholars have not paid enough attention to this problem. However,non-variceal vascular originated gastrointestinal hemorrhage is not uncommon,and is difficult and tricky in its management for clinicians. Therefore,clinicians should pay sufficient attention to the characteristics and therapeutic principles of non-variceal vascular originated gastrointestinal hemorrhage.

Objective To study the impact of tetramethylpyrazine on genes expression in colitis mice by means of cDNA microarray.Methods Thirty mice were divided into normal control group,0.9% NaCI solusion group and tetramethylpyrazine group with 10 in each group.Except the mice in normal group,all mice were treated with oxazolone enema,mRNA was extracted from colons of 0.9% NaCI solusion group and tetramethylpyrazine group.The eDNA probes were prepared and labeled with dyes Cy3 or Cy5.Then the fluorescent probe was hybridized with eDNA microarray and scanned for fluorescent intensity.The differently expressed genes were identified by analysis of gene expression profile.Two differently expressed genes (interleukin-4 and -10) were further validated by fluorogenic quantitative RT-PCR.Results There were 432 (2.86%) differently expressed genes in tetramethylpyrazine group.Of which,307 genes were up-regulated and 125 genes were down-regulated.The function of some genes were well known.Conclusions Tetramethylpyrazine,which can depress inflammatory reaction,anti-adhesive molecule and improve immune systems,is a potential therapy for ulcerative colitis although more detailed curing targes of such medicine need to be further study.

This study is to investigate the effect and its possible mechanisms of lidamycin (LDM) combined with tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) in human non-small cell lung cancer (NSCLC) cells. MTT assay was used to determine the growth inhibition of the two ingredients on H460 cells. Apoptosis was examined by Annexin V-FITC/PI staining, flow cytometry assay and DNA-specific dye Hoechst 33342 staining. The level of TRAIL receptor and apoptosis-associated protein expression was detected by Western blotting analysis. The results showed that the IC50 value of LDM and TRAIL for H460 cells was 4.603 x 10(-10) mol x L(-1) and 915.3 ng x mL(-1) respectively, but the IC50 value of LDM was 3.064 x 10(-11) mol x L(-1) and 1.611 x 10(-11) mol x L(-1) when different concentrations of LDM was combined with 50 and 100 ng x mL(-1) TRAIL respectively. And the CDI value was less than 1. The apoptosis ratios also increased in the combination group relative to the single-agent treatment and the untreated control. Furthermore, the induction of the cleavage of PARP and the activation of Caspase-3 and Caspase-8 by the combination were more effective than LDM or TRAIL alone. At last, the level of death receptor 5 (DR5) expressions increased in a dose-dependent manner and time-related pattern. The data indicate that LDM inhibits the growth of H460 cells in vitro. DR5 induction contributes to enhancement of TRAIL-induced apoptosis by LDM in human non-small lung cancer cells.

Objective To investigate the repairative effect of local high expression of glial cell line-derived neurotrophic factor (GDNF) in the repair of sciatic nerve defects. Green fluorescent protein (GFP) gene was used as reporter gene. Two groups including gel matrix control group and gel matrix plus MSCs group were set ( 10 rabbits per group). Methods GDNF secreting mesenchymal stem cells (MSCs) mediated by lentiviral vectors were suspended in Matrigel to produce the artificial graft, and then it was locally implanted to a 20 mm-long sciatic nerve defect in rabbits. Results In the GDNF group,the nerve continuity was restored in seven rabbits and the functional amelioration of the wounded limb was apparent. While there found no restoration of the nerve continuity in the control groups. The migration distance of GDNF secreting MSCs was 20 mm, suggesting a good integration of the transplanted cells with host cells. The glial fibrillary acidic protein (GFAP) + green fluorescent protein (GFP) and -100 protein GFP accounted for 60% and 70% respectively in the experimental group, while it was only 40% and 30% in gel matrix plus MSCs group. Conclusions Local implantation of GDNF secreting MSCs can promote the regeneration and functional recovery of the sciatic nerves. GDNF seems to play an important role in inducing transdifferentiation of MSCs to Schwann cell lineage.

Adult ; Humans ; Orthodontics, Corrective ; methods ; Periodontium