Objective To classify all the plant pollen allergens, each allergen sequence available was compared with protein family database. After that, the appearance and taxonomic distribution of each protein family could be known. When made reference to evolutionary analysis, a regular rule of the distribu-tion of all plant pollen allergens could be concluded. Methods Protein family memberships of each allergen were assigned by comparing the sequences with the Pfam database. Representative members of the most a-bundant pollen allergen families were compared with the Uniprot database using the BLAST server. Acces-sion number of all the interesting homologous could be obtained and all the sequence information could be ac-quired by Batch Entrez. Finally, the evolutionary trees were drawn with the help of MEGA4.0 software. Re-sults One hundred and sixty-eight pollen allergens were classified into 26 protein families. Profilins, pollen _allerg_1 and EF hands constituted the most abundant pollen allergen families. Profilins and EF hands oc-curred in almost all allergenic plant families, whereas allergenic Expansins, FAD_binding proteins, Amb_V allergens and Thaumatins were confined to single taxon. Conclusion It is concluded that the highly con-served sequences of allergens families such as Profilin may be one of the most important reasons of the cross reactivities in allergic diseases. The classification of pollen allergens may be helpful to clinical practice and basic research.

Objective To identify differentially expressed proteins in keloid tissue and to explore the proteomic characteristics of keloid by utilizing capillary high-performance liquid chromatography (LC) and tandem mass spectrometry (MS).Methods Tissue specimens were obtained from the lesions of 5 patients with non-familial keloid and normal skin of 5 human controls.After extraction and in-solution digestion of proteins,the resultant peptides were separated and identified by LC-MS/MS.Label-free detection and quantitation of peptides were carried out by using the DeCyder MSTM software.Results A total of 570differentially expressed peptides were identified between non-familial keloid and normal tissue (all P ＜ 0.05),which correspond to 1221 proteins.A difference greater than 1.5 folds was observed in the expressions of 293 proteins,including 124 up-regulated and 169 down-regulated proteins in non-familial keloid tissue.Conclusion Differences exist in the expression of proteins between non-familial keloid and normal skin tissue.

BACKGROUND:The good performance of resin cements used to cement fiber-posts has been confirmed, but the adhesive properties of different kinds of resin cements remain different.
OBJECTIVE:To compare the shear bond strength of three resin cements (Bisco One-step, Clearfil DC, 3M ESPE RelyX Unicem) used to cement fiber posts and to observe their adhesive properties.
METHODS:Fifteen extracted teeth were selected and the crowns were removed with a diamond bur 1-mm coronal to the cemento-enamel junction fol owed by endodontic treatment. Then the teeth were randomly divided into three groups (n=5). Glass fiber posts were cemented respectively with three resin cements, and after embedding, the roots were cut into 2-mm-thick sections for push-out tests. The mean shear bonding strengths were compared, and the failure modes were recorded and analyzed under a high-power microscope.
RESULTS AND CONCLUSION:Mean bonding strengths were (4.69±1.85) MPa for Bisco One-step group, Clearfil DC, (6.10±0.36) MPa for Clearfil DC group, and (7.04±0.92) MPa for 3M ESPE RelyX Unicem group. The bonding strengths of RelyX Unicem and Clearfil DC groups were significantly greater than that of Bisco One-step group (P<0.05), but no significant difference was between 3M ESPE RelyX Unicem group and Clearfil DC group. Al samples were mainly defined as modes Ⅱ andⅣfailure, indicating that the adhesive failure was the most mode of fracture. These findings showed that the bonding strengths of fiber posts cemented to root canals with three different resin cements system exhibit significant differences, and the interface between dentine and resin cements is the primary weak link.

Moyamoya disease is a cerebrovascular disease that is characterized by the progressive stenosis in the terminal internal carotid artery, the anterior cerebral artery and the origin of the middle cerebral artery, and compensatory enlargement of basal perforating artery. Both the genetic and environmental factors involxe in its pathogenesis. but its exact cause is unknown. Revascularization is the main treatment approach for moyamoya disease. This article reviews the progress in research on epidemiology, etiology, diagnosis and treatment of moyamoya disease.

Gastrointestinal stromal tumor(GIST) ,a kind of rare tumor,takes 1%～3% of all tumors in digestive tract,but are the most common in mesenehymal neoplasm.At present,the primal treatment of GIST is surgical operation,standard and rationality of surgery is the significant fatter that affects its curative effect.While imatinib is the main therapeutic tool for GIST that can' t benn resected or recurrence after operation.There is no definite circumscription between optimum and malignancy,there are many factors that influence the prognosis of GIST.We commonly apply Fletcher grading to evaluate the risk of GIST.

Objective To observe the efficacy of Lipo PGE combined with Shuxuetong injection in treatment of diabetic peripheral neuropathy (DPN). Methods The 68 DPN patients were randomly divided into treatment group with Lipo PGE plus Shuxuetong injection and comparison group with Shuxuetong alone. The treatment period was 14 days. Results The pain, numbness,paresthesia and other symptoms in the limbs of patients were significantly reduced in treatment group than in comparison group (P＜0. 01). At the same time, the conduction velocity of median nerve and sural nerve was significantly increased in treatment group after treatment (P＜0.05), there were no significant differences in comparison group before and after the treatment. Conclusions The treatment of DPN with Lipo PGE combined with Shuxuetong injection is safe and effective.

Objective To observe the expression of intercellular adhesion molecule 1(ICAM-1)and vascular cellular adhesion molecuh 1(VCAM-1)in placenta in patients with hypertensive disorders in pregnancy,investigate their roles in patients with hypertensive disorders in pregnancy and correlation.Methods Fifty-two patients with hypertensive disorders in pregnancy(disease group)were collected,including 11 csses with gestational hypertension(GH),18 cases with mild pre-eelampsia(MP)and 23 cases with gevere pre-eclampsia(SP),26 cases of normal pregnant women as control group.Immunohistochemistry and image analytical technique were employed to show the expression of ICAM-1 and VCAM-1.Results The quantities of ICAM-1 and VCAM-1 expressed in the placental vascular endothelial cell were 78.85%(41/52)and 75.00%(39/52)in disease group,26.92%(7/26)and 23.08%(6/26)in control group,there was a significant difference between the two groups(P<0.01).and those in SP patients[100.00%(23/23)and 100.00%(23/23)]were higher than those in MP patients[77.78%(14/18)and 72.22%(13/18)]and GH patients[36.36%(4/11)and 27.27%(3/11)](P<0.05 or<0.01).The quantities of ICAM-1 and VCAM-1 expressed in the placental villous syncytlotrophoblast were cell 38.46%(20/52)and 44.23% (23/52)in disease group,100.00%(26/26)and 96.15%(25/26)in control group,there was a significant difference between the two groups(P<0.01),and those in SP patients[8.70%(2/23)and 13.04%(3/23)]were lower than those in MP patients[44.44%(8/18)and 50.00%(9/18)]and GH patients[90.91%(10/11)and 100.00%(11/11)](P<0.05 or<0.01).Conclusion Abnormal expression of ICAM-1 and VCAM-1 in the placental vascular endothelial and villous syncytiotrophoblast cell in patients with hypertensive disorders in pregnancy may participate in pathogenic process of vascular endothelial damage of hypertensive disorders in pregnancy.

The microarray technology used in biological and medical research provides a new idea for the diagnosis and treatment of cancer. To find different types of cancer and to classify the cancer samples accurately, we propose a new cluster ensemble framework Dual Neural Gas Cluster Ensemble (DNGCE), which is based on neural gas algorithm, to discover the underlying structure of noisy cancer gene expression profiles. This framework DNGCE applies the neural gas algorithm to perform clustering not only on the sample dimension, but also on the attribute dimension. It also adopts the normalized cut algorithm to partition off the consensus matrix constructed from multiple clustering solutions. We obtained the final accurate results. Experiments on cancer gene expression profiles illustrated that the proposed approach could achieve good performance, as it outperforms the single clustering algorithms and most of the existing approaches in the process of clustering gene expression profiles.
Algorithms ; Cluster Analysis ; Gene Expression Profiling ; methods ; Humans ; Neoplasms ; genetics ; Transcriptome

Objective:To explore the correlation between lymph node metastasis rate and prognosis in non-small cell lung cancer (NSCLC) patients with stage Ⅲ A/N 2. Methods:The clinical data of 350 NSCLC patients with stage Ⅲ A/N 2 receiving surgical treatment from January 2012 to June 2015 in Shanxi Provincial Cancer Hospital were retrospectively analyzed. The patients were divided into the high lymph node metastasis rate group and the low lymph node metastasis rate group according to the optimal cutoff value of lymph node metastasis rate determined by Cox hazard regression model. The relationship between clinicopathological features and lymph node metastasis rate was analyzed. Prognostic risk factors were analyzed by using Cox regression model, and overall survival (OS) and disease-free survival (DFS) of both groups were analyzed by using Kaplan-Meier method. Results:The optimal cutoff value of lymph node metastasis rate was 0.2. There were 180 cases with high lymph node metastasis rate (>0.2) and 170 cases with low lymph node metastasis rate (≤0.2). The proportion of the patients with adenocarcinoma and the highest lymph node metastasis in the high lymph node metastasis rate group was higher than that in the low lymph node metastasis rate group [72.2% (130/180) vs. 52.9% (90/170), 52.8% (95/180) vs. 29.4% (50/170), all P<0.05]. The proportion of patients with jumping N 2 lymph node metastasis, single station N 2 lymph node metastasis and single station N 2 lymph node metastasis in the highest group was lower than that in the low lymph node metastasis rate group [51.1% (92/180) vs. 71.8% (122/170), 25.0% (45/180) vs. 44.1% (75/170), 38.9% (70/180) vs. 75.3% (128/175), all P<0.05]. Cox multi-factor regression model analysis showed that adenocarcinoma, multiple stations N 2 lymph node metastasis, lymph node metastasis rate were independent risk factors of DFS for NSCLC patients with stage Ⅲ A/N 2 ( HR = 2.201, 95% CI 1.444-3.355; HR=2.971,95% CI 1.950-4.529; HR=3.543, 95% CI 1.874-6.699; all P<0.05). Lymph node metastasis rate was an independent risk factor of OS for NSCLC patients with stage Ⅲ A/N 2 ( HR = 3.669, 95% CI 1.941-6.938, P<0.05). The 5-year OS rate of the low lymph node metastasis rate group was higher than that of the high lymph node metastasis rate group (64.00% vs. 36.58%, χ2 = 11.422, P = 0.001). The 5-year DFS rate in the low lymph node metastasis rate group was higher than that in the high lymph node metastasis rate group (45.00% vs.18.32%, χ2 = 13.624, P<0.01). Conclusion:Lymph node metastasis rate is an independent influencing factor for the prognosis of NSCLC patients with Ⅲ A/N 2 stage, and it can effectively evaluate the prognosis.

Objective Observe effects of TIMP-3 gene transient expression on proliferation,migration and apoptosis of VSMCs.Methods Transfect VSMCs separately in three groups as control,empty plasmid and recombinant group mediated by cationic liposome.First,calculate cells,quantify MMP-2 and analyse TIMP-3 mRNA at 24,48 and 72 h;Second,measure cell apoptosis rate and calculate migrated cells as well after 48 h.Results There were no difference between control group and empty plasmid group in cell growth,MMP-2's content,TIMP-3 mRNA expression and apoptosis rate,cells migration rate;but those of recombinant group were greatly different in other two(P