Laparoscopic and robotic techniques change the view of groin anatomy from inside to outside.The amplification view of laparoscopy extends surgeons' visual field,with clear and precise anatomy,less bleeding and certain outcome.Robotic flexible joints increase angle of suture to abdominal wall.In this background,hernia surgeons pay close attention to closure of abdominal wall defect,choice and fix of mesh as well as status of robotic technique in hernia filed.

Female ; Hernia, Inguinal ; etiology ; pathology ; surgery ; Humans ; Male ; Risk Factors

Many specific therapeutic antibody drugs have been developed for different indications. In drug development, it has been found that the antibody isotype framework can not only affect the physical and chemical properties of therapeutic antibodies, but also influence the activity and therapeutic effect. As a result, IgG isotype selection should be considered carefully in antibody drug development strategies. Because of the unique biological characteristics, IgG4 isotype has been used in some therapeutic antibodies for which effector functions are not desired. In order to provide new ideas for the development of antibody drugs, the research and application progress of IgG4 isotype in therapeutic antibody drug development has been reviewed.
Drug Design ; Humans ; Immunoglobulin G ; chemistry ; pharmacology

Adult ; Female ; Health Knowledge, Attitudes, Practice ; Humans ; Hygiene ; Male ; Middle Aged ; Occupational Health ; Young Adult

Objective To investigate whether emergency rooms in major general public hospitals are reasonably designed and convenient for patients. Methods 78 major public hospitals were selected from all over China, except Hong Kong, Macau and Taiwan, for emergency room convenience analysis. The standards in use were the "Standards of Hospitals" of Joint commission International Accreditation. Results The investigation found in such hospitals the following: poor road conditions outside the clinic and insufficient guides in the clinic; long-than-expected distance (normally over 50 meters) from ER to imaging departments (such as X-ray and CT scan service); ERs in provincial capital cities are significantly more convenient than those in non-capital cities; distance from ERs to CT service is significantly shorter in hospitals in eastern China than those middle and western China. Conclusions Parameters on ER's service quality from "Standards of Hospitals" could serve as a guideline to emergency medicine in China. ERs in China need to improve with conspicuous ER signs, unblocked passageways, and convenient access to inspection departments.

Objective To explore the abnormal inflammatory cytokine profile of salpingitic infertility by means of cytometric bead assay(CBA). Methods To assay representative inflammatory cytokines of 40 patients of salpingitic infertility(PSI) and 40 healthy control by CBA, and compared 30 samples of serum with peritoneal fluid collected from identical subject. Results Experiment data displayed that in PSI the Th lymphocytes were decreased significantly (t =2. 1236,P <0. 05), Ts lymphocytes increased markedly, and the ratio of Th/Ts markedly decreased ( t = 2. 8392, P < 0. 01 ), compared with those of healthy control.(Pre-)inflammatory eytokines (IL-8, IL-1β, IL-6 and TNF-α) were all significantly higher than those of healthy controls ( t = 4. 9302,5. 5449,3. 4975,5. 6623, respectively, all P < 0. 01 ) ; while anti-inflammatory cytokines ( IL-10, IL-12 ), also increased markedly ( t = 5. 3428,5.9847, respectively, all P < 0. 01 ).Beside IL-10, all other cytokine concentrations presented in serum were higher than that of peritoneal fluid;IL-8(t =2.2113,P <0.05),IL-llS(t =2.9756,P <0.01), IL-6(t =2.3298,P <0.05), TNF-α (t =3. 2895, P < 0. 05 ), IL-12 ( t = 3. 3281, P < 0. 05 ), implying more sensitive of serum samples. Conclusion PSI displayed extensive disorders of the inflammatory cytokine network, CBA can be used to probe the disharmony condition at high through-out and high sensitivity.

BACKGROUND: Transplantation of bone marrow mesenchymal stem cells (MSCs) improves functional recovery after spinal cord injury (SCl), but the mechanisms involved remain unclear.OBJECTIVE: To observe the effects of MSCs transplantation on the expression of brain-derived neurotrophic factors (BDNF) in rats after SCl.DESIGN, TIME AND SETTING: Randomized, controlled, animal experiment. The study was performed at the Laboratory of Neuroanatomy, China Medical University from April to July 2003.MATERIALS: A total of 64 SD rats, aged 3 months, of either gender, weighing 250-300 g, were used. Of them, 4 were randomly selected to isolate and culture MSCs, and the remaining were used to establish SCl models.METHODS: The 60 rats were randomly divided into 3 groups. Seven days after SCl, MSCs group (n=24) was transplanted with 5 μL culture solution containing 1×109/L MSCs to the injury site using micro-injection; PBS group (n=24) was transplanted with 5μ L PBS, and the blank control group (n=12) with 5μ L normal saline.MAIN OUTCOME MEASURES: The rats were sacrificed at 7, 14, and 28 days post-surgery. MSCs morphology was observed and the expression of BDNF at the lesion areas was examined by immunohistochemistry,RESULTS: All 60 rats were included in the final analysis. After ten subcultures, the cell proliferative capacity was reduced, and cell body turned to flat; the MSCs protiferation and morphous could be maintained by adding basic fibroblast growth factor.Transplantation of MSCs enhanced the expression of BDNF compared with PBS and blank control groups at 7, 14, and 28 days post-surgery (P ＜ 0.05); white no significant difference was found between PBS and blank control groups (P ＞ 0.05).

Objective To investigate the short-term treatment effectiveness of the auditory integrative training(AIT) on intelligence, language and gross motor function in children with spastic cerebral palsy (CP).Methods Sixty patients with spastic CP, aged 2 to 4 years old,who had been given all the routine conventional rehabilitation at the Rehabilitation Department of the People's Hospital of Guangxi Zhuang Autonomous Region from January 2011 to December 2013 were randomly divided into AIT treatment group (30 cases) and control group without AIT (30 cases), all patients were investigated by using Gesell Developmental Scales and the Gross Motor Function Measure (GMFM)-66 before and after 3-month therapy, and the changes in the regional cerebral blood flow of children (rCBF) were detected by single photon emission computed tomography (SPECT) before and after treatment.Results The scores of development quotient (DQ) of the treatment group after the AIT in areas of adaptive behavior, personal-social behavior,language(52.44 ± 13.43,52.07 ± 11.57,57.19 ± 6.18) were higher than those of the control group (45.09 ± 11.02,45.86 ± 9.66,53.44 ± 5.49), and the differences between the 2 groups were greatly significant statistically(t =-2.32,-2.26,-2.49, all P ＜ 0.05).There were no significant difference in DQ of gross motor, fine motor and scores on the GMFM-66 in the treatment group before and after treatment [40.40 ± 8.57,49.50 ± 14.20, (55.81 ±8.72) scores vs 44.03 ±11.90,46.34±9.78,(58.81 ±7.86) scores,t=-1.42,1.08,-0.52,all P＞ 0.05].SPECT detected abnormalities in all patients (100.00％) both in the 2 groups, compared with the control group,the rCBF was improved significantly after treatment in the treatment group (86.67％) (x2 =35.49 ,P ＜ 0.05).Conclusions The treatment of AIT can greatly improve the intelligence, language development and the brain function in children with spastic CP.It is an effective adjutant of rehabilitation method for children with spastic CP to improve intelligence and language development, and has less influence on motor function.

Objective To explore the role of CGRP and progesterone along with it on the etiopathology of PIH. Methods 8 specimens of umbilical vein endothelial cells from women with PIH were cultured. Four groups were divided when cultured cells were confluent: the sera of normal pregnancy women (group 1), the sera of women with PIH (group2), the mixture of the sera of women with PIH and the injection of progesterone (group3), and the same amount of media (group 4). The cultured cell were added respectively into the four different kinds of media. After 24h of incubation, the concentration of endothelin (ET) and CGRP in media were measured by radioimmunoassay. Results 1. Comparing with group1, the concentration of CGRP in group 2 decreased and ET increased significantly. Statistic difference of ET/CGRP was found between the two groups. 2. Comparing with group 1, the concentration of CGRP in group 3 was unchanged and ET increased obviously. No difference of ET/CGRP was shown between them. 3. Comparing with group 3, in group 2, the concentration of CGRP decreased obviously and that of ET unchanged. Significant difference of ET/CGRP was found. Conclusions Progesterone, along with CGRP, might delay the onset and progressing of PIH.

Through the research and practice of computer basic course at medical colleges, the paper summarizes the experience of using modern technical means, to reform the traditional teaching ideas, teaching methods, teaching means and teaching management, to diversify teaching resources sharing. It also analyzes the shortcomings and makes recommendations to further strengthen the course development.