Objective To compare the therapeutic efficacies between conventional acupuncture plus acupoint sticking and conventional acupuncture alone in treating allergic rhinitis.Method Sixty patients were randomized into two groups, 30 cases in the treatment group and 30 cases in the control group. The treatment group was intervened by conventional acupuncture plus acupoint sticking, while the control group was intervened by conventional acupuncture alone. The symptoms grading scores were evaluated before and after the treatment to compare the scores of sneezing, runny nose, stuffy nose and itchy nose of the two groups, and the therapeutic efficacies were compared.Result The total effective rate was 96.7% in the treatment group, significantly higher than 80.0% in the control group (P<0.05);after the intervention, the symptoms scores in the treatment group were significantly lower than those in the control group (P<0.05), indicating that the therapeutic efficacy of the treatment group was superior to that of the control group. Conclusion Conventional acupuncture plus acupoint sticking can effectively improve the symptoms of allergic rhinitis, and its therapeutic efficacy is superior to that of the conventional acupuncture.

Objective To investigate the clinical efficacy of different waveform electroacupuncture in treating convalescence-stage peripheral facial paralysis.Methods Ninety-four patients with convalescence-stage peripheral facial paralysis were randomly allocated: 22 cases to treatment group 1, 25 cases to treatment group 2, 23 cases to treatment group 3 and 24 cases to the control group. The same acupoints were selected in different groups. Treatment group 1 received electroacupuncture with continuous waves; treatment group 2, electroacupuncture with sparse-dense waves, treatment group 3, electroacupuncture with interrupted waves; the control group, simple electroacupuncture. Treatment was given once every other day, five times as a course. The Sunnybrook Facial Grading System score was recorded in every group before and at one and three months after treatment. The clinical therapeutic effects were compared between the groups.Results The total efficacy rate was 86.4% in treatment group 1, 88.0% in treatment group 2, 82.6% in treatment group 3 and 62.5% in the control group. There was a statistically significant difference in the total efficacy rate between each of the treatment groups and the control group (P<0.05). There was no statistically significant difference in the total efficacy rate between the treatment groups (P>0.05). In each of the treatment groups, there was a statistically significant difference in the Sunnybrook score between before treatment groups and at one or three months after treatment (P<0.05). There was a statistically significant difference in the Sunnybrook score at one and three months after treatment between each of the treatment groups and the control group (P<0.05). There was a statistically significant difference in the Sunnybrook score at one month after treatment between treatment group 3 and treatment group 1 or 2 (P<0.05).Conclusions Different electroacupuncture treatments all have a marked clinicaleffect on convalescence-stage peripheral facial paralysis. Electroacupuncture with interrupted waves is more effective in restoring facial muscle function in the early stage of convalescence.

Objective To explore the effect on efficacy and safety of Wuling capsule on patients with post-stroke depression.Methods From July 2012 to September 2014, 90 post-stroke depression patients were selected in our hospital.The patients were divided into treatment group (n=45) and control group (n=45) by random number table method.The post-stroke depression patients in control group were treated with fluoxetine as clinical drug program.The post-stroke depression patients in treatment group were treated with Wuling capsule as clinical drug program, six weeks for a course of treatment, both group were treated after two courses,the clinical indicators improve effect, clinical treatment, changes of cytokines, incidence of adverse reactions of the patients between two groups were compared and analyzed.Results Hamilton depression scale (HAMD) scores and Barther index (BI) scores of patients in treatment group post-treatment were better than control group (P<0.05) .Compared with control group, the ratio of clinical efficiency and total efficiency significant in treatment group were increased significantly (P<0.05).The patients in treatment group post-treatment, the levels of cytokine of interleukin-6 (IL-6) and tumor necrosis factor-α(TNF-α) decreased significantly (P<0.05) .Compared with control group, the incidences of adverse effect of patients in treatment group were decreased (P<0.05).Conclusions Wuling capsule has certain therapeutic advantages for improving clinical efficacy and reducing adverse reactions for patients with post-stroke depression.It could as the therapy of choice for post-stroke depression patients.

Objective:To establish a headspace GC method for the determination of 7 kinds of residual solvents in bicalutamide, including dichloromethane, n-hexane, tetrahydrofuran, ethanol, ether, acetone and ethyl acetate. Methods: The residual solvents in the substance were determined by GC equipped with an FID detector and linked with an Agilent DB-624 capillary column (30. 0 m × 0. 25 mm × 1. 4 m). The inlet temperature was 200℃ and the FID detector temperature was 250℃. The column temperature was raised by program:the initial temperature was 35℃, maintained for 13 min, raised to 180℃ with a rate of 100℃/min, and maintained for 5 min. The carrier gas was nitrogen and the flow rate was 1. 2 ml·min-1. The heated temperature of the headspace oven was 90℃, the heated time lasted 30 min, and the injection volume was 1. 0 ml. The solution medium was dimethyl sulphoxide (DMSO). Results:Each solvent could be completely separated, and the calibration curve of each solvent showed good linear relationship with good accuracy. Conclusion:The method can be applied for the determination of residual solvents in bicalutamide.

[Objective] The purpose of this study was to evaluate the association of ultrasound findings and different treatments with the prognosis of the pump twin in twin reversed arterial perfusion sequence (TRAP).[Methods] The 55 cases that were diagnosed as TRAP during the period from January 4th 2010 and August 31st 2015 were included.Ultrasound appearances of monochorionic twins were documented in detail when the twins were diagnosed with TRAP initially.At the initial evaluation,presence or absence of the following abnormalities were documented,including the ratio of the acardiac twin to the weight of the pump twin,hydrops fetalis in the pump twin,cardiomegaly in the pump twin,polyhydramnios in the pump twin,oligohydramnios in the acardiac twin and different treatments.The relationship between these ultrasound characteristics and different treatments with mortality of pump twin were analyzed with logistic regression.[Results] A total of 55 cases with TRAP were diagnosed in our institution and three cases were lost to follow up.Selective reduction was performed in 21 cases,termination of pregnancies in 13 and expectant management in 18.Excluding the cases that underwent termination of pregnancy,the remaining 39 cases were divided into two groups according treatment,expectant management group (n=18) and selective reduction group (n=21).Overall survival rate of the pump twin in these two groups was 66.7％ and 71.4％,respectively,P =0.748.The markers of perinatal outcome were not significantly different between expectant management group and selective reduction group.Logistic regression demonstrated that cardiomegaly in the pump twin (OR=21.73,95％CI:1.15 ～ 203.89,P ＜ 0.001) were significant correlated with mortality of the pump twin.[Conclusion] The prognosis markers were not significantly different between conservative management and selective reduction group.Cardiomegaly in the pump twin should be considered as risk predictors for mortality of the pump twin in TRAP.

Objective To detect the anti EZH2 autoantibodies IgG expression in hepatocellular carcinoma patient's serum samples and investigate the relationship of anti EZH2 autoantibodies IgG expression with clinicopathological parameters.Methods ELISA method was used to detect the anti EZH2 autoantibodies in serum of 120 cases of HCC patients and compared with 115 healthy controls.Results Serum anti EZH2 antibodies in patients with hepatocellular carcinoma group the content of IgG was 2.015±0.568 μg/ml group,anti EZH2 antibodies IgG content control health was 1.185±0.289 μg/ml.There was significant difference between two groups (t=12.48,P＜0.001).Anti EZH2 antibodies of IgG in serum of pa ticnts with hepatocellular carcinoma was up-regulated and the degree of tumor size,tumor the degree of differentiation,TNM grading and intrahepatic metastasis (t=8.485 ～ 57.93,P＜0.001).And age,sex,drinking,whether HBV infection had no significant difference (t=1.22～5.20,P＞0.05).Conclusion The expression of anti EZH2 autoantibodies in serum was related to the occurrence and development of hepatocellular carcinoma,and the detection of serum anti EZH2 autoantibodies in serum of liver cancer may be helpful to judge the malignant behavior and prognosis of hepatocellular carcinoma (HCC).which may be used as a refcrcnce for the evaluation of the prognosis of HCC patients with IgG antibody against IgG.

Objective To investigate the effect of intratracheal instillation of curosurf on neonatal respiratory distress syndrome ( NRDS ) in children with right ventricular function.Methods 52 patients with NRDS were retrospectively selected and divided into two groups according to different treatment.The patients in the conventional group were treated with nasal airway ventilation.Based on the conventional group, the curosurf group was taken with curosurf.26 cases were in each group.The blood gas index (PaCO2, PaO2, pH), inflammatory reaction (TNF-α, IL-10), SF of the two groups were compared, the complications and curative effect of the two groups before and after treatment were taken for statistics.Results There was no significant difference in pH value between the two groups at each time point.The PaO2 expression levels in the curosurf group at different time points after treatment were significantly lower than the conventional group (P<0.05).The levels of TNF-αand IL-10 in the curosurf group were more stable than those in the control group at different time points after treatment (P<0.05), and the degree of SF increasing at different time points were higher (P<0.05).The total effective rate 80.77% of the curosurf group was significantly higher than that of the conventional group 61.54%(P<0.05).The total complication rate 19.24% had no significant differences with the conventional group 23.08%.Conclusion Intratracheal instillation of CsA in the treatment of NRDS has the advantages of simple operation, little side effect, rapid recovery of blood gas index and inflammatory factors, so it is a feasible method for clinical treatment of NRDS.

Objective　To study the effects of hypoxia and glutamic acid on the kinetic properties of NMDA receptor channel of the hypothalamic neurons in rats. Methods　Cell-attached mode patch clamp technique was employed to record the single channel current of the NMDA receptor. Results　The open probability of NMDA receptor channel was increased after acute hypoxia compared with that of normal state, the open time τ1,τ2 was changed from (0.33±0.10)ms，(4.36±0.26)ms to (0.93±0.22)ms，(7.64±0.72)ms, and the close time τ1,τ2 was from (18.03±3.50)ms，(171.50±19.10)ms to (3.42±1.02)ms，(19.39±3.07)ms. The mean open probability was changed from 0.12±0.05 in normal state to 0.66±0.36 in hypoxia state. Furthermore, glutamic acid can increase open time and open probability of NMDA receptor channel,decrease close time. Conclusion　The excitability and the open probability of NMDA receptor channel of hypothalamic neurons increased by hypoxia is related to glutamate.

Objective To study the effect of extracts of Smilax china L. on inhibition the experimentally induced benign prostatic hyperplasia ( BPH) , and screen the effective fraction. Methods The BPH model was built on the castrated rats by subcutaneous injection of testosterone propionate. Male rats were randomly divided into eight groups ( n=6 ):sham operation, model control, petroleum ether fraction, acetic ether fraction, n-butyl alcohol fraction, water fraction, macroporous resin fraction ( FMR) , and total extracts group. The rats were treated with testosterone propionate by subcutaneous injection for consecutive 3 weeks. Meanwhile, rats were orally administrated with the six extract fractions of S. china L. After the last administration, serum was separated for the determination of prostatic acid phosphatase ( PACP ) , prostate was weighed and histopathological examination was carried out to evaluate the inhibitory effect of S. china L. against BPH. Results All of the six fractions from S. china L. could inhibit BPH, and the n-butanol fraction, water fraction and FMR showed better inhibitory effect, which significantly decreased the prostatic index by 52. 80%, 50. 93% and 67. 70%, respectively, remarkably reduced serum PACP, and notably improved the prostate gland morphology compared with the model group. Among the three fractions, FMR showed the strongest effect against BPH. Conclusion S. china L. ameliorates the experimentally prostatic hyperplasia, and FMR showes the best effect, which might be the bioactive components against BPH.

Objective To investigate serum anti-insulin-like growth factor-Ⅱ mRNA binding protein1(IMP1) autoantibody IgG expression in hepatocellular carcinoma(HCC) patient and its relationship with clinicopathological parameters.Methods ELISA method was used to detect serum anti-IMP1 autoantibody IgG in 120 cases of HCC,then the detection results were compared those in 115 healthy individuals(control group).The relationship between serum anti-IMP1 autoantibody IgG level with clinicopathological parameters in HCC patients was analyzed.Results The serum anti-IMP1 autoantibody IgG levels in the HCC group and control group were(1.512±0.685),(1.080±0.301)μg/mL respectively,the HCC group was significantly higher than the control group with statistical difference(P<0.001);serum anti-IMP1 autoantibody IgG in HCC patients was related with the tumor size,tumor differentiation degree,TNM grade and intrahepatic metastasis(P<0.001),and had no obvious relation with the age,sex,drinking and HBV infection(P>0.05).Conclusion Serum anti-IMP1 autoantibody IgG expression increased is increased in HCC patient.The detection of serum anti-IgG autoantibody IMP1 level in HCC is conducive to judge the malignancy degree of HCC.