0.05); the effective rates in rest pain in the two groups were 41.25% and 57.97%,respectively(P

OBJECTIVE:To establish an RP-HPLC method for the determination of Berberine hydrochloride in Keyang-min tincture.METHODS:The sample separation was performed on Agilent Eclipse XDB C18(250 mm?4.6 mm,5 ?m),and the mobile phase was acetonitrile-5 mmol?L-1 perfluorooctane sulfonate(32∶68) at flow rate of 1 mL?min-1.The detection wavelength was 346 nm and the temperature of column was 35 ℃.RESULTS:The linear range of Berberine hydrochloride was 0.059 2～0.888 3 ?g(r=0.999 9).The average recovery rate was 99.71%(RSD=1.18%,n=6).CONCLUSION:The met-hod is sensitive,accurate,and reproducible,and suitable for the determination of Berberine hydrochloride in Keyangmin tincture.

OBJECTIVE:To study the effect of Hordeum vulgare on hormone level in male mice.METHODS:Male mature mice(5～6 months) were treated with Hordeum vulgare decoction 1 mL(equivalent to 0.5 g?mL-1 crude drug) bid for 14 consecutive days.Follicle-stimulating hormone(FSH),luteinizing hormone(LH),estradiol(E2) and testosterone(T) in blood were monitored.RESULTS:In Hordeum vulgare group compared normal control,serum E2 increased significantly,serum level of testosterone decreased significantly,while FSH and LH levels remained stable.CONCLUSION:Hordeum vulgare can influence the hormone level in male mice by influencing the gonad axis of the mice.

OBJECTIVE:To explore the reason for the "tragedy of anti-commons" in biomedical patent and its effect on the development of biomedical industry.METHODS:The mathematical models of "commons" and "anti-commons" gambling were established based on the analysis on emergence of "patent bush" resulted from the abuse of biomedical patents,and the profit conflict of the patents power related to elementary researcher and applicant researcher was analyzed.RESULTS:The potential "tragedy of anti-commons" affects the enthusiasm of the biomedical researchers,hinders the rapid development of biomedical industry and the improvement of technological level.CONCLUSIONS:The corresponding measures should be taken from aspects such as system,research model,and patent applicant model to balance the possessiveness and exclusiveness,avoid the "tragedy of anti-commons" and facilitate the development of biomedical industry.

Antibodies, Monoclonal ; therapeutic use ; Antibodies, Monoclonal, Humanized ; Antineoplastic Agents ; therapeutic use ; Antineoplastic Agents, Hormonal ; therapeutic use ; Antineoplastic Agents, Phytogenic ; therapeutic use ; Breast Neoplasms ; drug therapy ; pathology ; ultrastructure ; Carcinoma, Ductal, Breast ; drug therapy ; pathology ; ultrastructure ; Chemotherapy, Adjuvant ; Female ; Humans ; Neoadjuvant Therapy ; Nitriles ; therapeutic use ; Taxoids ; therapeutic use ; Trastuzumab ; Treatment Outcome ; Triazoles ; therapeutic use

Objective: To probe into the relationship between changes of sex hormone levels and TCM syndrome differentiation in climacteric women.Methods: According to TCM syndrome differentiation,55 cases of women in menopause were divided into three groups: kidney yang deficiency,kidney yin deficiency and the normal;the correlation between the three types and E2,FSH and LH was explored by statistical methods,and then they were compared with procreative women.Results: In all the 55 cases,kidney yin deficiency covered 70.90%,kidney yang deficiency covered 16.36%,the normal covered 12.72%;changes of E2 correlated positively with symptom scores of kidney yang deficiency and kidney yin deficiency;changes of FSH and LH correlate negatively with that of kidney yang deficiency and kidney yin deficiency.And they all correlated closely with menopause.Conclusion: the main syndrome type of climacteric women was kidney yin deficiency;E2↓,FSH↑ and LH↑ was expression of physiological kidney deficiency of climacteric women.

Objective To study the influence of pine-seed oil upon body weight,blood lipid and lipid peroxidation of mice fed on high fat feed.Method The mice were fed by high fat feed(80% basal feed added 20% lard,HF) and HF added pine-seed oil(80% high fat feed added 20% pine-seed oil,SHF).At 45 d and 90 d,the body fat content,blood lipid level,and malonyldehyde(MDA),lipofusin(LP),catalase(CAT),superoxide dismutase(SOD) and glutathione peroxidase(GSH-Px) of brain and liver homogenate were measured respectively.Results Pine-seed oil could reduce the body weight serum triglyceride(TG),total cholesterol(TC),low density lipoprotein-cholesterol(LDL-C) content,but increase high density lipoprotein-cholesterol(HDL-C) of the mice fed by HF.It suggested that pine-seed oil had definite effect on regulating blood lipid.Compared with normal control(NC) group,the activities of CAT、T-SOD、GSH-Px of brain and liver in HF group were remarkably lower,but MDA and LP remarkably higher.It suggested that HF could promote lipid peroxidation in brain and liver of mice.Compared with HF group,the activites of CAT,T-SOD and GSH-Px of brain and liver in SHF group were remarkably higher,but MDA remarkably lower.Conclusion Pine-seed oil can reduce the body weight,blood lipid,and lipid peroxidation of mice fed by HF.

OBJECTIVE: To study the bioequivalence of domestic oxcarbazepine dispersible tablets. METHODS: 20 healthy male volunteers were randomly given oxcarbazepine dispersible tablets (test tablet) and oxcarbazepine tablets (reference tablet) via p.o. The plasma concentration of active metabolite (MHD) of oxcarbazepine were determined by HPLC. 3p97 software was used to calculate pharmacokinetic parameters and the bioequivalence of oxcarbazepine were evaluated. RESULTS: After treatment main pharmacokinetic parameters of test tablet vs. reference tablet were as follows: Cmax(4.231?0.841) ?g?mL-1 vs. (4.350?0.861) ?g?mL-1; tmax(4.13?0.43) h vs. (4.28?0.44) h; t1/2(14.17?2.66) h vs. (14.44?2.09) h; AUC0～60 (113.00?22.25) mg?h?L-1 vs. (118.11?14.20) mg?h?L-1; AUC0～∞(126.35?20.94) mg?h?L-1 vs. (130.54?16.17) mg?h?L-1. The relative bioavailability of test tablet to reference tablet was (95.67?12.80)%. CONCLUSION: Two kinds of tablets were bioequivalent.

Objective To understand the plasma Platelet Microparticiles(PMPs)level and its clinical significance in patients with type 2 diabetes mellitus(T2DM).Methods Venous blood samples were obtained from 96 of T2DM patients and 30 of non-diabetic control subjects.PMPs,CD61P positive on platelets,were quantified by flow cytometry.Meanwhile,plasma levels of PLT,FPG,TC,TG,HDL-C,LDL-C and LP(a)were detected,as well as the levels of MA and TRF from urine samples.Results PMPs counts were significantly greater in 2 year or up 2 year groups than in controls.PMPs count was associated with levels of urine MA and TRF,but not related with other indexes.Conclusion PMPs may be a marker of microvascular disease in Type 2 diabetes.