Objective To detect the salivary levels of IgA,alpha-amylase and acetylcholinesterase (AChE) and saliva flow rates of IgA in normal controls versus Parkinson disease (PD) patients,and to analyze the correlation of these salivary biochemical indexes with disease duration,H&Y rating scale,SCOPA-AUT score,sialorrhea score,dry mouth score and the use of levodopa.Methods Thirty-eight PD patients including twenty-two patients treated with levodopa,and twenty-one normal controls were selected in our study.About 3 ml saliva was collected from each subject.Salivary IgA was detected by immunoturbidimetry.Alpha-amylase and AChE were tested by enzymatic colorimetry.The protein concentration was analyzed by BCA kit to normalize ACHE.The salivary flow rate and IgA flow rate were also calculated.Results Salivary flow rate (ml/min) was (0.39±0.22) in normal controls,and (0.35±0.28) in PD groups;the level of IgA (mg/L) was (148.3±86.1) in normal controls,and (183.7±126.3) in PD groups;IgA flow rate (μg/min) was (47.8±25.9) in normal controls,and (46.9±27.1) in PD groups;the level of alpha-amylase (U/L) was (1.63 ± 1.21) × 105 in normal control,and (1.35 ± 9.87) × 105 U/L in PD group;and the level of AChE (U/mg) was (0.12±0.08) in normal controls,and (0.09±0.05) in PD group.No statistically significant differences in above indexes were found between PD group and controls.There were negative correlations between salivary flow rate and dry mouth score (r =-0.445,P =0.005) and between alpha-amylase concentration and sialorrhea score (r=-0.327,P=0.045).The positive associations were found between IgA concentration and the SCOPA-AUT score (r =0.438,P =0.006),and between IgA flow rate and sialorrhea score (r =0.411,P =0.01).Conclusions Salivary flow rate and level of alpha-amylases tend to be decreased in PD patients,while IgA concentration has an upward tendency.Salivary alpha-amylases concentration and IgA flow rate are correlated with the symptom of sialorrhea,while IgA concentration correlates with the degree of autonomic symptoms.These measurements can be used as indexes for evaluating the lesions of the autonomic nervous system.

Splenic cyst is clinically rare.It can be classified as either true or false.Ultrasound,CT and MRI scan are helpful in diagnosing splenic cyst.Indication for a treatment depends mainly on clinical symptoms and diameter of the cyst.Now,there is a more conservative attitude on splenic surgery and the patient should be offered laparoscopic approach.Both nonoperatively treated patients and surgically treated patients should be given ultrasound examination regularly,in order to check the cyst size and exclude reappearance.

Objective The relationship between the clinicopathological features and the prognosis of breast cancer patients was evaluated by the expression of EPB41L4A-AS2 in breast cancer tissues.Methods The relationship between the expression of EPB41L4A-AS2 and the clinical features of breast cancer was evaluated by using the genome meta analysis,TCGA and Gene Expression Library(GEO) datasets.The correlation between EPB41L4A-AS2 and apoptotic pathway was verified by Western blotting.Results The results from Meta -analysis,TCGA and GEO datasets showed that EPB41L4A-AS2 was low in breast cancer tissues and was positively correlated with poor clinical and pathological features.EPB41L4A-AS2 was confirmed an association with the classical apoptosis pathway in breast cancer cell lines.In the meta-analysis of GEO,we found the high expression of EPB41L4A-AS2 with good prognosis.Conclusion EPB41L4A-AS2 inhibits tumor formation and has a high value in clinical prognosis of breast cancer.

Objective:To observe the clinical efficacy of acupuncture plus turtle-shell-partitioned moxibustion in treating diarrhea- predominant irritable bowel syndrome (IBS-D). Methods:Seventy-six patients were randomized into two groups: 39 cases in the observation group were intervened by acupuncture plus turtle-shell-partitioned moxibustion, while 37 cases in the control group by the same acupuncture treatment, once a day, 10 d as a treatment course, for 2 courses in total. The improvements of symptoms were observed at the end of the intervention in both groups. Results:The total effective rate was 89.7% in the observation group versus 67.6% in the control group, and the difference was statistically significant (P<0.05).Conclusion:Acupuncture plus turtle-shell-partitioned moxibustion can significantly improve the symptoms of IBS-D, and can produce a more significant efficacy than acupuncture alone.

Objective To explore the efficacy and safety of transcatheter intra-arterial hyperthermo-chemotherapy for advanced ovarian carcinoma. Methods 36 patients with unresectable or recurred ovarian carcinoma were divided into two groups randomly: treatment group(n=20) and control group(n=16). Transcatheter intra-arterial infusion warmed to 62 degree chemo-agents via the ovarian tumor supplied artery was performed in the treatment group while routine intraarterial chemotherapy was done in the control group. The CA125 level dynamic change, short-term efficacy and side effect after the interventional procedure were compared between two groups.Results The CA125 level decreased significantly in the treatment group more than that in the control group(?

Intracellular stimulating and recording method was used by two glass microelectrodes on crayfish small axon. Physiologic and physical parameters of small nerve fiber were measured. 4—aminopyridine (4-AP). a selective inhibitor of the potassium channel,made"b" (an irritability index,a constant in Weiss's formula) decreased(p

Objective To investigate the influence of nicotine on the metabolism of dopamine (DA) in the striatum and on the spontaneous locomotor activity of MPTP-induced PD mice. Methods 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced PD model in mice was prepared. The effects of MPTP and nicotine on the levels of dopamine, its metabolites of 3,4-dihydroxyphenlacetic acid (DOPAC) and hemovanillic acid (HVA) were analyzed with high-performance liquid chromatography and electrochemical detection(HPLC-EC); also, tyrosine hydroxylase (TH)-positive nerve fibers were measured by immunohistochemical analysis. Results After injection of Nicotine, the spontaneous locomotor activity of MPTP-treated C57BL mice was significantly lower than that of the control mice, suggesting the nicotine's inhibiting effects ( 44.86?8.51 vs. 57.28?3.27, P

Objective To understand the house-dust mite fauna in the school dormitory in Guangzhou city.Methods With the direct smear method and saturation solution of NaCl floatation method,a total of 970 bed-dust samples collected from the five school dormitory buildings were examined,from Oct.to Dec.in 2006.The house-dust mite in the samples were counted and classified.Results The prevalence rate of mite was 98.76%.About 72% of the samples,the number of mites was 500 and 3 000/g dust.Mean number of the mites in bed-dust samples showed a significant difference between the male and female student dormitory,and among five dormitory buildings.The mean number of mites usually decreased as the floor increased.Dermatophagoides pteronyssinus,Dermatophagoides farinae,gamasid mites,scab mites and pyemotidae etc were found in the samples.Conclusion House-dust mites universally exist in bed-dust of the school dormitory in Guangzhou city and the number is larger.Dermatophagoides pteronyssinus,Dermatophagoides farinae are the dominant kinds among the mites.

OBJECTIVE:To compare the efficacy and safety of only paroxetine vs. paroxetine combined with alprazolam in the treatment of diabetes complicated with anxiety and depression. METHODS:Totally 86 patients with diabetes complicated with anxi-ety and depression were randomly divided into observation group and control group. The patients in observation group were given paroxetine 20 mg,qd,and alprazolam 0.4 mg,tid;patients in control group were given paroxetine alone. The treatment course lasted for 8 weeks in 2 groups. The clinical data was compared,including fasting plasma glucose(FPG),2 h postprandial glucose (2 h PG),glycated hemoglobin (HbA1c),cortisol,adrenocorticotropic hormone (ACTH) levels and scores of Hamilton anxiety scale (HAMA) and Hamilton depression scale (HAMD). The adverse reactions were observed. RESULTS:After treatment,FP-BG,2 h PG,HbA1c,cortisol,ACTH levels and scores of HAMA and HAMD in observation group were significantly lower than control group,with significant difference(P<0.05). There were significant differences in the incidence of adverse reactions be-tween 2 groups(P>0.05). CONCLUSIONS:Compared with paroxetine alone,paroxetine combined with alprazolam can improve more in blood glucose,endocrine levels and adverse mood symptoms in the treatment of diabetes complicated with anxiety and de-pression,with similar safety.

AIM:To find out the effect of oxidative stress on macrophage colony-stimulating factor (M-CSF) gene expression in macrophages.METHODS: The effect of tert-butyl hydroperoxide (tbOOH) on M-CSF expression in RAW264.7 cells was investigated by RT-PCR method.RESULTS: The results showed that tbOOH (1 5?10 -4 mol/L) could induce M-CSF mRNA expression in RAW264.7 cells; and the induction became greater with increase in tbOOH concentration. Furthermore, the induction of M-CSF in RAW264.7 cells by tbOOH could be attenuated by cycloheximide or acetovanilone added in the medium.CONCLUSION: The results indicated that tbOOH could transcriptionally induce M-CSF expression in RAW264.7 cells, and de novo protein synthesis and intracellular superoxide (O  2) production might be involved in the process.