BACKGROUND: Cecropins are a kind of micromolecule protein with antibacterial activity. Eukaryotic cell-expressed or artificially synthesized Cecropins is characterized by low efficiency and high cost. OBJECTIVE: To clone and express an antibacterial peptide gene of Musca domestica Cecropin A, and to identify recombinant expression product. METHODS: Mature Musca domestica Cecropin A encoding nucleotide sequence was searched from the GenBank and amplified by RT-PCR. The gene of Musca domestica Cecropin A was cloned into prokaryotic expression vector pET32a and fused with gene of Thioredoxin (Trx) and expressed in E.coli BL2l (DE3). After induction by isopropyl-β-D-thiogalactoside, the sera of the immunized rabbits were collected after rabbits were immunized with the hemolymph of housefly larvae. Recombinant protein was identified by western blot analysis and N-[Tris(hydroxymethyl)methyl]glycine-sodium dodecylsulfate-polyacrylamide gel electrophoresis.RESULTS AND CONCLUSION: After induction by isopropyl-β-D-thiogalactoside, E.coli BL21 expressed mature Cecropin. Rabbit anti- housefly larvae sera, N-[Tris(hydroxymethyl)methyl]glycine-sodium dodecylsulfate-polyacrylamide gel electrophoresis and western blot analysis results confirmed that expression products were mature Cecropin. These suggest that prokaryotic expression system can be utilized to obtain natural mature Cecropin.

MicroRNA (miRNA) regulate the expression of target mRNA according to the specific base pairing. They are involved in proliferation,differentiation and apoptosis of cells. The expression of miRNA has distinct differences between normal tissues and cancers, miRNA can be oncogenes but also suppressor genes, they play an important role in cancer. And miRNA are promising to be a new therapeutic target. In this review, we will discuss the advance in the research of miRNA in cancer.

Objective: To observe the clinical effects of needling acupoints Shenting(GV 24),Neiguan(PC 6) and Sanyinjiao(SP 6) in the treatment of migraine. Methods: Thirty cases of Germen patients with migraine were treated mainly by Shenting(GV 24), bilateral Neiguan(PC 6) and Sanyinjiao(SP 6), as well as bilateral Hegu(LI 4) and Taichong(LR 3), Touwei(ST 8),Yangbai(GB 14), Cuanzhu(BL 2), Sizhukong(TE 23), Shuaigu(GB 8) and Sibai(ST 2) in the affected side of head, auricular Sympathetic (MA-AH 7), Er Shenmen (MA-TF 1), Heart (MA-IC) and Endocrine (MA-IC 3); another 28 cases were treated by routine acupuncture. The clinical results and immediate pain-relieving effects were observed. Results: The total effective rate was 90.0% in treatment group and 75.0% in control group, χ2= 4.57, P ＜ 0.05; the 24-hour pain-relieving effects were obviously better in treatment group than that in control group, χ2 = 10.11, P＜ 0.01. Conclusion: Needling Shenting(GV 24), Neiguan (PC 6) and Sanyinjiao(SP 6) has better effects than routine acupuncture in the treatment of migraine.

Objective:To observe whether the in-flow anesthetic gas absorber can reduce the recovery time after stopping inhalation of enflurane or isoflurane. Methods: In fixed tidal volume, minute volume and fresh gas flow, the recovery time of isoflurane or enflurane anesthesia with or without in-flow anesthetic gas absorber were compared after closing the vaporizer.Results: With in-flow anesthetic gas absorber, the time for anesthetic gas concentration in circle to reduce the MAC to 0.3was 3.3±0.5 min for isoflurane, which was significntly shorter than that without absorber (20±0.3min) ( P＜0.01 ) and the time for enflurane was 3.5±0.5min and 25±0.1min respectively. Conclusion:Anesthetic gas absorber can reduce the recovery time of either enflurane or isoflurane inhalational anesthesia significantly.

Lung cancer is the most common cancer worldwide, accounting for approximately 18%of all cancer-related deaths. Circulating tumor cells (CTCs) have the capacity to spread into the blood stream. This capacity of CTCs is one of the most critical fac-tors for unfavorable clinical outcome of patients with NSCLC. Detection of CTCs using a simple blood test may assist in early detec-tion of lung cancer. Researchers have attempted to isolate CTCs from peripheral blood using various techniques. Although reports on CTC detection are abundant, methodological aspects concerning sensitivity, specificity, and reproducibility have prevented a clear ap-praisal of the clinical impact. This paper gives a brief description of each CTC detection method.

Cell Research got all impact factor of 3. 426, which was the highest Chinese SCI journal, but ranked 4th by journal's h index. By analyzing the cites and citations of Cell Research from 1999-2006 using the method of bibliometrics, four main characteristics of Cell Research were found:①The quantity of papers published Oil Cell Research is low and their quality is hish. ②Advantageous opening, with authorship patterns, editorial members and peer reviewers from home and abroad. ③Journal published in English is easy to perform international academic exchange. ④International operation model also played an important role in Cell Research's success.

Objective To study the effects of violet flower Bidai extracts on body weight,blood lipid level,serum leptin level,the activity of lipoprotein lipase and hepatic lipase of obese rats,and to discuss the mechanism of violet flower Bidai extracts on weight reduction.Methods Male obese rats which induced by high energy diet were randomly divided into four groups.All rats were given high energy diet.One control group was gavaged with normal saline and one western medicine group was gavaged with sibutramine,and two violet flower Bidai extracts groups was gavaged with water solutions of violet flower Bidai extracts at doses of 6 g(crude drug)/kg BW,12 g(crude drug)/kg BW,respectively.After six weeks,body weight,obese index,weight of abdominal fat tissue,fasting lipid,leptin in serum,the activity of lipoprotein lipase and hepatic lipase were measured.Results Body weight,obese index,weight of abdominal fat tissue in the two violet flower Bidai extracts groups were significantly lower than those in obese control group(P

0.05).The splicing destruction among each group was the main breakage method between materials and fiber posts.The surface treatment of fiber posts with sandblast or hydrogen peroxide significantly enhanced the bond strength of the composite resin tested.The surface treatment of fiber posts with silane did not enhance the bond strength of the composite resin.

Statins may not only lower cholesterol concentration,reduce the incidence of heart diseases and relieve kidney damage,but also stimulate bone formation obviously,as suggested by a number of observation studies and animal researches.However,the exact mechanism of promoting bone formation has not been very clearly yet.Moreover,their adverse effects and their interaction with other drugs still need further clinical trials.In this review,on the basis of clinical data about the effects of hydroxymethylglutaryl-CoA(HMG-CoA)reductase inhibitors,we summarize current hypotheses on their mechanism and safety,in order to provide reference for the domestic bone reconstruction research.

Objective To understand the freshmen’s status of hepatitis B virus infection,immunological effect after vaccina-tion against hepatitis B from a university in Ankang city,to provide a scientific basis for university’s prevention from hepati-tis B.Methods Analyzed the results of five markers of hepatitis B virus infection and alanine aminotransferase (ALT)of 25 895 freshmen in a college in Ankang for resent 12 years,and observed the HBsAb serocoversion in serum 1 year after vacci-nation against hepatitis B and after strengthened immunity.Results The HBeAg positive rate of the freshmen was 4.07%, among which the male was 5.58% (281/5 035),and the female was 3.14% (258/8 214).The difference had significance of statistic (P<0.05).The college students’HBeAg positive rate from rural area and from urban area were 4.77% and 2.93%respectively,the difference had significance of statistic (P<0.05).There was no significant statistic difference in immune effect of hepatitis B vaccine for different sex (χ2=5.80,P<0.05).Their HBsAb positive rate was 95.15% after injected with 10μg hepatitis B and 5μg hepatitis B vaccine,which had significance of statistic (P<0.05).Conclusion The hepatitis B virus infection rate and the hepatitis B protective antibody of the college freshmen in Ankang district was lower than that of the whole country and had a decreased tendency.It showed the male’s infection rate of hepatitis B virus was higher than the female,the rural was higher than the urban,and the immune effect of hepatitis B vaccine proved to be good.It should be-strengthened immunity for non-responders to ensure the immune effect if necessary.