OBJECTIVE:To investigate effect of valsanan on heart function in patients with chronic heart failure(CHF).METHODS:A total of 113 CHF patients were assigned into 2 groups.Both group received routine therapy and observation group were additionally treated with valsanan.Clinical efficacies of 2 groups before and after treatment were observed in terms of cardiac function classification,LVIDD,SV,CO,LVEF and 6 min walking test.RESULTS:In observation group,the incidence of improvement of the cardiac function was higher than in control group(P

Objective To investigate the effect of emulsified isoflurane preconditioning on focal cerebral ischemia-reperfusion (I/R) injury in rats and the mechanism. Methods Fifty-six healthy male adult SD rats weighing 250-280 g were randomly divided into 4 groups (n = 14): group Ⅰ sham operation (group S); group Ⅱfocal cerebral I/R; group Ⅲ emulsified isoflurane preconditioning and group Ⅳ fat emulsion preconditioning. Focal cerebral ischemia was induced by middle cerebral artery occlusion (MCAO) using a nylon thread with rounded tip inserted into internal carotid artery and advanced cranially until resistance was met. MCAO was maintained for 2 h followed by 24 h reperfusion. In group Ⅲ and Ⅳ 8% emulsified isoflurane 7.5 ml/kg and 3% fat emulsion 7.5 ml/kg were injected intraperitoneally at 24 h before MCAO respectively. Neurologic outcome was evaluated at 24 h of reperfusion and scored (0 = no deficit, 4 = unable to crawl, loss of consciousness). The animals were then killed and brains removed. The infarct size was assessed. The apoptotic cells were detected by TUNEL and calculated. The expression of Bax, Bcl-2, cytochrome C and caspase-3 protein was detected by immunohistochemical staining. Results The neurologic deficit scores were significantly lower in emulsified isoflurane preconditioning group than in I/R group. Preconditioning with emulsified isoflurane significantly decreased the infarct size and the number of apoptotic cells and increased the expression of Bcl-2 protein and inhibited the expression of Bax, cytochrome C and caspace-3 protein. Conclusion Emulsified iscflurane preconditioning can protect the brain from focal cerebral I/R by increasing Bcl-2 protein expression and decreasing Bax protein expression, and reducing cytochrome C release from mitochondria, caspase-3 activation and neuronal apoptosis.

OBJECTIVE:To probe into the status quo and requirements of drug utilization in Haiqin Sanatorium Area of Nanjing Military Region Hangzhou Sanatorium.METHODS:The drug utilization in 710 convalescents in Sanatorium Area from May 2007 to April 2008 was analyzed statistically.RESULTS:A total of 10 categories of drugs were used in the 710 convalescents,chiefly including anti-cold drugs,antihypertensive drugs,drugs for external use,antimicrobial agents,ophthalmic drugs,otorhinolaryngological drugs,vitamins and gastrointestinal drugs.The rate of drug use conforming diagnosis was 87.32%.Oral administration occupied 71.13%.CONCLUSION:The drug utilization in convalescents in Haiqin Sanatorium Area is basically rational.However,there are also some unreasonable points in the drug utilization which remain to be tackled further.

Objective To evaluate the clinical values of human papilloma virus(HPV)testing in screening for women of early cervical cancer lesion.Methods 282 cases of cervical screening of patients had received cervical exfoliated cells collection,at the same time for a liquid thin-layer cytology test(TCT)and HPV genotyping detection.Abnormal results received histologic examina-tion,the results regarded as the diagnostic gold standard by pathological examination.Results In HPV DNA positive group(126 cases),by liquid based cytology,NILM,ASCUS,LSIL,HSIL and SCC were found in 53,22,32,18,1 cases,respectively.His-topathologic examination positive rate was 42.86%.In HPV DNA negative group(156 cases),there were 133 cases with NILM,16 cases with ASCUS,and 7 cases with LSIL.Histopathologic examination positive rate was 11.54%.Two groups of patients with pathologic positive rate compared,the difference was statistically significant(P <0.01 ).High risk type HPV detection rate in-creased with the increased degree of cervical lesions.Conclusion Women of early cervical cancer and precancerous lesions in the screening line HPV detection has important significance.

Establishing harmonious physician-patient relationship is importamt to set up the harmonious society.Some hospitals try in recent years a line the single disease grows the price control charges,which suggest its importamt role in seting up harmonious physician-patient relationship,and greatly attract public's concern as the new measure in this aspect.

OBJECTIVE: To establish the HPLC method for the determination of chloramphenicol and dapsone in compound chloramphenicol cream. METHODS: The analytical column was Hypersil ODS and the mobile phase consisted of 0.1%sodium heptanesulfonate solution (a mixture of 0.1% sodium heptanesulfonate 500 mL and dimethylformamide 5 mL plus acetic acid glacial 0.5 mL)-acetonitrile (75∶25) at a flow rate of 1.0 mL?min-1. The detection wavelength was 272 nm and the sample size was 20 ?L. RESULTS: The linear ranges of chloramphenicol and dapsone were 25.59～153.54 ?g?mL-1 (r=0.999 9) and 12.57～75.42 ?g?mL-1 (r=0.999 9), respectively. The average recovery rates were 100.62% (RSD=0.92%) and 100.93% (RSD=1.22%), respectively. CONCLUSION: The method is simple and accurate and can be used for the quality control of compound chloramphenicol cream.

It is the traditional virtue of Chinese to be honest, and honest-trust is the foundation of the development of market economy. The phenomenon of honest-trust lacking in the field of medical care is a comprehensive result for many factors. It's a new subject to advocate sincere service and spread honest-trust culture for the construction of medical ethics. Therefore, we should put emphasis on the medical ethics education, strengthen the consciousness of sincere service, perfect the criteria of medical ethics, and set up the credit system of medical organization and medical staff.

Objective To observe the efficacy of Lipo PGE combined with Shuxuetong injection in treatment of diabetic peripheral neuropathy (DPN). Methods The 68 DPN patients were randomly divided into treatment group with Lipo PGE plus Shuxuetong injection and comparison group with Shuxuetong alone. The treatment period was 14 days. Results The pain, numbness,paresthesia and other symptoms in the limbs of patients were significantly reduced in treatment group than in comparison group (P＜0. 01). At the same time, the conduction velocity of median nerve and sural nerve was significantly increased in treatment group after treatment (P＜0.05), there were no significant differences in comparison group before and after the treatment. Conclusions The treatment of DPN with Lipo PGE combined with Shuxuetong injection is safe and effective.

Objective To explore the influence of vacuum sealing drainage(VSD) technology in snake bite treatment.Methods 108 patients of snake bites need to incision decompression were randomly divided into two groups,VSD group (VSD) 58 cases,conventional group (wound change dressings ordinarily) 50 cases.The secondary suture of time,the number of change dressings,material consumption fees,antibiotics use cost,wound infection rate,in hospital,average length of hospital stay were compared between two groups.Results Compared the VSD group with the conventional group,the secondary suture of time (t =11.743,P ＜ 0.05),the number of change dressings (t =50.661,P ＜ 0.05),material consumption fees (t =9.054,P ＜ 0.05),antibiotics use cost (t =21.515,P ＜ 0.05),wound infection rate (x2 =9.155,P ＜ 0.05),in hospital,average length of hospital stay (t =10.350,P ＜ 0.05) had significant differences.Conclusion VSD technique can be a ideal way to treat snake bites patients and worth popularizing at this stage.

Due to the complicated and changeable clinical symptoms,and inexact pathogenesis,leukemia was facing huge challenges in diagnosis and therapy.Aptamer could be selected through CELL-SELEX.The aptamer not only display high affinity and specificity to target cell,but also it could discriminate the specific stage of cell,such as different classification,normal and tumor cells.So CELL-SELEX has great potention in the researches of leukemia.This paper systematically summarized the advantages of CELL-SELEX,the application in specific leukemia diagnosis,targeted therapy and the discovery of cancer biomarkers.(Chin J Lab Med,2013,36:377-379)