Objective To investigate the effect of 5‐fluorouracil on hypertrophic scar in rabbit ears .Methods The hyper‐trophic scar models in rabbit ears were reproduced and randomly divided into 3 groups (group A ,group B and group C) .On postop‐erative 60th days ,Hematoxylin‐eosin and Masson staining staining was applied to observe themorphological and collagen change of scars under light microscope .Real‐time PCR and ELISA were conducted to detect the expression of TGF‐β,PDGF ,bFGF and INF‐γ .Results HE and Masson staining showed that thinner dermis ,fewer fibroblasts and collagen fibers ,collagen fibers arranged reg‐ularly and less collagen nodule was observed group B compared with the group A and group C .Furthermore ,we found that TGF‐β, PDGF ,bFGF ,INF‐γmRNA and protein expression levels were significantly lower in group B and group C than the group A ,and group B was more effective than group C .Conclusion 5‐fluorouracil is effective for the treatment of hypertrophic scars ,and the lo‐calized injection of 5‐fluorouracil is more effective than the ointment group .

Objective To investigate the correlation between epicardial fat thickness (EAT) and the risk factors of coronary artery disease (CAD) as well as the severity of coronary lesions with echocardiography,Methods According to the results of coronary angiography,80 patients underwent coronary angiography were divided into coronary heart disease group (CHD group,4 6 cases) and without coronary heart disease group (control group,34cases),and according to coronary artorrythe severity,CHD goup was divided into subgroups of single-vessel disease (24 cases) and multivessel disease subgroup(22 cases).Tho epioardial fat thickness on the free wall of the right ventricle was measured at end-systole from the para-sterna,long-axis vlews.The relationship between EAT and the risk factors of CAD (such as age,gender,hypercholesterolemia,hypertenston,diabetes,smoking,family CAD history) as well as the severity of coronary lesions were analyzed.Results EAT in multivessel disease CAD group [(8.85 ± 1.44)mm] was significantly higher than that in single vessel CAD group[(6.86 ± 1.09) mm] and control group [(4.58 ± 1.12) mm] (all P ＜ 0.01).EAT was significantly related with the risk factors of CAD.Although the risk factors of CAD such as age,blood glucose,blood pressure were adjusted,EAT was still independently associated with CAD (OR:5.394,95 ％ CI:1.700 ～ 17.1 12,P =0.004).Concluslon The level of EAT is significantly correlated with CAD and the severity of coronary lesions.

Catch-up growth,a phenomenon that occurs after a short time growth-inhibiting condition,has been proved to be strongly associated with insulin resistance.But the mechanism of insulin resistance in the process of catch-up growth is not clear.

The model of vaginal candidiasis in Kunming mice was constructed in order to search for the optima construction conditions and provide an economic animal model of Candida albicans (C. albicans) vaginitis. Estrogen benzoate (E2) was given to mice at different concentrations ranging from 0.0 to 0.05 mg/mouse (4 levels) beginning 72 h prior to vaginal inoculation, then mice were inoculated intravaginally with various concentrations of stationary-phase C. albicans blastoconidia (ATCC90028) (5 levels) in 20 microL of phosphate-buffered saline (PBS) in each E2 level. General state, scores of genital pathology, the hyphae and vaginal fungal burden (CFU) in vaginal lavage fluid, the hydrops rate of uterus and vaginal tissues for pathological section in mice were observed and obtained at day 2, 4, 7, 14 and 21 after inoculation. The results showed the infection rate in mice was related to the dosage of E2 and concentration of C. albicans blastoconidia. Additionally there was better cross-effect between the two treated factors. The infection rate was about 80% on the day 4, and could reach 100% on the day 7 until the end of experiment after inoculated intravaginally in groups of E2I3, E2 0.025 mg/mouse injected hypodermically and inoculated intravaginally with 5 x 10(4) C. albicans blastoconidia, and large amount of hyphae and blastoconidia could be observe in superficial layer tissue and canal of vaginal by PAS. From the results in our experiment it was concluded that E2I3 was the optima construction condition in kunming mice.

To investigate the effects of the free radical, 1,1-Diphenyl-2-picylhydrazyl, on cochlear blood flow, 20 guinea pigs were divided into 3 groups at random, 6 for control group, 6 for 1 mmol/L group and 8 for 0.1 mmol/L group. 2 microliters vehicle or drugs were dropped into round window membrane (RWM). Cochlear microcirculation was monitored by laser Doppler flowmeter (LDF), and mean arterial blood flow (MABP), which was transferred by pressure conductor sensor and preamplifier, was simultaneously recorded on the computer. Our results showed that MABP was stable throughout the experiment. Cochlear blood flow (CBF) increased by 10.32% (P < 0.05) in 1 mmol/L group, and decreased by 4.89% in 0.1 mmol/L group (P < 0.05). In control group cochlear microcirculation showed no significant changes. It is concluded that DPPH exerted effects on cochlear microcirculation.
Blood Flow Velocity ; Cochlea/*blood supply ; Free Radicals/pharmacology ; Laser-Doppler Flowmetry ; Microcirculation/drug effects ; Picrates/*pharmacology ; Random Allocation

The traditional view that vitamin D is mainly involved in bone metabolism and calcium and phosphorus homeostasis,and bone-related diseases such as osteoporosis related.However,some recent studies have shown that vitamin D plays a key role in obstetrics and gynecology diseases,particularly,endometriosis and ovarian cancer,preeclampsia and gestational diabetes mellitus (GDM) in polycystic ovary syndrome influence (PCOS).Advances in vitamin D article and on the relationship between gynecological and obstetrical diseases were reviewed.

Noninvasive continuous positive airway pressure (CPAP) for the ventilation treatment of obstructive sleep apnea-hypopnea syndrome (OSAHS) is the first-line treatment method. This article aims to describe the effect of surgical operation especially for the UPPP in the individualized comprehensive treatment of OSAHS and the importance of surgical technique. Lower compliance is the bottleneck of CPAP therapy in clinical application, for the OSAHS patients with treatment failure in CPAP or those cannot accept CPAP therapy, when with no other ideal instrument therapeutics, accurate diagnosis of position in airway obstruction with an adequate surgical operation is the treatment,of choice. Surgical operation is particularly important either as a fore-lying means to improve the CPAP treatment compliance or as an independence treatment method of OSAHS. The pharyngeal cavity is the most common obstructive plane in patients with OSAHS. The operation of traditional UPPP aiming at the expansion of pharyngeal cavity is the classics surgery to solve obstruction in this plane, the lower operation effective rate is the main reason of restriction in its development. How to improve the effective rate of surgical treatment of OSAHS is our surgical goal. The effective rate of surgical operation treatment in OSAHS rely on the following sides: to follow the OSAHS individualized comprehensive treatment principle, reasonable choice of surgical operation indication, the precise localization diagnosis of upper airway obstruction, adequate surgical operation and skilled surgical techniques.
Airway Obstruction ; Continuous Positive Airway Pressure ; Humans ; Palate ; surgery ; Pharynx ; surgery ; Sleep Apnea, Obstructive ; surgery ; Treatment Failure

BACKGROUND:Bone defects often occur after the reduction of periarticuar fractures, and bone grafting is required to fil bone defects, thereby to make the early support of articular surface and prevent the col apse and shift of the articular surface. Al ograft cancellous bone is the transplant material for the treatment of bone defects, but its osteogenesis ability is poor. Autologous red marrow has osteogenesis ability. Therapeutic efficiency of al ograft cancellous bone combined with autologous red marrow for periarticular fractures is stil yet to be
assessed.
OBJECTIVE:To study clinical effect of locking plate fixation and al ograft cancellous bone combined with autologous red marrow in the treatment of periarticular fractures.
METHODS:Forty-three cases of periarticular fractures were incorporated into the Orthopaedics Department of the Third Hospital of Hebei Medical University. After cutting the articular surface anatomical y, composite particles of the red marrow and al ograft cancellous bone were implanted into bone defects, and then, an anatomic locking plate was used. Medial lateral or bilateral locking plate was used for tibial plateau fractures. Dorsal or volar locking plate was used for distal radius fractures, and distal tibial medial or lateral locking plate was used for distal tibial fractures.
RESULTS AND CONCLUSION:Forty-three patients were fol owed up for 12 months to 6 years, an average of 4.3 years. X-ray films and CT scans review showed that 43 patients’ col apse fracture al reached bony union. Fresh fracture healing time was 2-6 months, an average of 4 months;the healing time for old fracture was 3-7 months, an average of 5.5 months. After bone grafting, 43 patients al had no significant immune rejection, two cases showed more wound exudates, and the wound was healed by dressing after 2 weeks. One case had wound infection, the wound was healed after 4 weeks of draining and dressing, and the infection had no recurrence fol owing up for 4 years and 1 month. Forty patients were satisfied with bone grafting, accounting for 93%, and three cases dissatisfied, accounting for 7%, based on Mankin and Komender’s standard assessment. The results confirmed that al ograft cancellous bone combined with autologous red marrow transplantation can play a supporting role in treating periarticular fractures to prevent the col apse of the articular surface and fracture displacement, and to provide reconstruction materials for periarticular defects. Its long-term goal is fracture healing.

[Objective]To discuss the clinical effect of Fanggan decoction on H1N1 Influenza virus.[Method]Randomly divided 95 cases into 2 groups,treatment group 48 and control one 47.Treatment group takes Fanggan decoction,the control one uses reducing fever tablet or anti-virus drugs if needed.[Result]The effective rate was 89.6% for treatment group and 85.1% for the control one.The treatment group was better than the control one on the reducing fever time,cough duration and cold symptom time.[Conclusion]Fanggan decoction has a good effect on H1N1 influenza virus.It also can be one of the therapies to relieve influenza patients in Zhejiang Province.

Objective To analyze clinical manifestation and identify prognostic factors in patients with acute respiratory distress syndrome (ARDS) caused by cytomegalovirus (CMV) pneumonia after kidney transplantation.Methods Fifty five kidney transplant recipients' data were analyzed in a case-control study of patients with a primary discharge diagnosis of ARDS caused by CMV pneumonia.Results The mortality of ARDS after kidney transplantation was 56.4%;when association with mechanical ventilation,it was 72.5%.In multivariate Logistic regression analysis,numbers of complications (odds ratioOR2.60,95% confidence intervalCI1.00~6.76),severity of diffuse infiltrates in chest X-ray (OR 11.83,CI 1.14~123.07),mechanical ventilation (OR 11.83,CI 1.14~123.07) were independent risk factors to death.Conclusion Even in modern era,kidney transplant recipients with ARDS after kidney transplantation are still at high risk for death.Numbers of complications,severity of diffuse infiltrates in chest X-ray,mechanical ventilation are associated with poor outcomes.