Five hundred elderly patients with ischemic stroke admitted from January 2008 to December 2010 were enrolled in the study,including 352 recurrent cases and 148 primary cases.There was a positive correlation of stroke recurrence with age,blood pressure,blood glucose and lipid levels,smoking and alcohol consumption of patients(P ＜0.05 ); while the stroke recurrence was negatively correlated with physical exercise(P ＜ 0.05).Stepwise multiple regression analysis indicated that the blood pressure,blood glucose and lipid levels were risk factors for the recurrence of ischemic stroke in elderly patients.

Sperm associated antigen 9(SPAG9),a new member of the c-Jun NH2-terminal kinase (JNK)interacting protein(JIP)family. plays a role in sperm-egg fusion and mitogen-activated protein kinase (MAPK)signaling pathway.Researches have shown that SPAG9 is closely related to the biological behavior of human malignant tumors, and plays an important role in the development of tumor.

This paper introduces the perioperative nursing of 47 patients undergoing intraperitoneal parastomal hernia repair.After the surgery,two cases suffered from incision infection and two cases with subcutaneous hydrops.Three patients suffered from chronic pain or foreign bodies sensation,which disappeared at 3-6 months later.No recurrence occurred at 6-24 months after the surgery.It is suggested that the key points of perioperative nursing were careful preoperative symptom care and bowel preparation,as well as postoperative observation and correct discharge instruction.

Objective To compare the effectiveness and advantage of salvage liver transplantation post-resection with primary liver transplantation for HCC patients. Methods Medline, Embase, Cochrane, CBMdisc, CNKI and Wanfang Database were searched. Randomized and nonrandomized controlled trials comparing primary liver transplantation with salvage liver transplantation were analyzed. Ten retrospective studies involving 1115 patients were included. 164 received salvage liver transplantation,and 951 patients received primary liver transplantation. Results In most researches, the two groups of patients were similar for age, sex,and cirrhosis severity at time of transplantation. Some researches showed no difference in tumor size and number between the two groups. Few studies referred to microvascular invasion. Most of the researches showed there was no significant difference in blood loss,operative time,overall survival and disease-free survival between the two groups. Conclusion Compared to primary liver transplantation, salvage liver transplantation after liver resection is associated with similar operative time, risks and overall survival. Resection first and salvage liver transplantation for recurrent tumors or liver failure seem to be a feasible treatment strategy for hepatocelluar carcinoma,especially in early-stage.

Objective To study some sensitive electrophysiological parameters in surveillance of allograft rejection.Methods Forty rats underwent heterotopic heart transplantations.IMEG was re- corded by an epicardiac unipolar pacing lead fixed at the right ventricular outflow tract.QRS amplitude and heart rate were determed daily in 10 syngeneic and 30 allogeneic transplants.Syngeneic transplants were killed at 7 th postoperative day,and allogeneic transplants killed at 3 rd,5 th and 7 th postopera- tive day.Histopathologie studies were performed at every transplanted heart.Results In syngeneic group,QRS amplitude kept constant after the transplantation while no significant differences were ob- served at the 3 rd,5 th and 7 th postoperative day.QRS amplitude was dropped obviously in allogeneic group after the first two postoperative days whereas significant differences were observed at the rejec- ting and non-rejecting hearts.Conclusions IMEG is a valid method to monitor acute allograft rejec- tion.QRS amplitude is a more sensitive electrophysiological parameter to diagnose severe rejections than heart rate,while mild rejections were not detected by this method.

Objective:To observe the expression of Clusterin in colorectal cancer tissues and study its relationship with the development and progression of colorectal cancer.Methods:Fifty-eight colorectal cancer tissues samples(32 rectal cancer tissues,26 colon carcinoma tissues) of patients with completed clinic data were obtained after surgery from the Affiliated Hospital of Guangdong Medical College.Immunohistochemical staining(SP method) was used to detect the expression of Clusterin in colorectal cancer tissue,adjacent normal tissue of colorectal cancer and normal tissue.Flow cytometry was used to examine the apoptosis of colorectal cancer cells.Results:The expression of Clusterin in colorectal cancer tissues was significantly higher than those in the adjacent tissues of colorectal cancer and normal colorectal tissue(P

To determine the relationship between Barrett's esophagus (BE) and features of gastroesophageal acid reflux, 24 h esophageal pH monitoring was performed in 90 patients. The patients were divided into 3 groups: 31 subjects with BE, 21 with mild esophagitis and 38 with severe esophagitis. The following parameters were evaluated: the percentage time of pH < 4; the number of reflux episodes over 5 min; the duration of longest episodes and DeMeester score over total period and the auterior three parameters in erect and supine position. All these parameters in BE were significantly different from those with mild esophagitis (P < 0.01) and not significantly different from those with severe esophagitis (P > 0.05). During supine position all the above parameters in BE were significantly different from those with reflux esophagitis (P < 0.05). It is concluded that the quantity of acid reflux is not an important factor in development of BE in gastroesophageal reflux (GER), and the acid reflux in supine position might be important in development of BE in GER.
Barrett Esophagus/etiology ; Barrett Esophagus/*physiopathology ; Esophagitis, Peptic/etiology ; Gastroesophageal Reflux/*complications ; Hydrogen-Ion Concentration ; Supine Position

Objective To explore the relationship between the expression of MutS homolog2 (MSH2) and CyclinD1 proteins in breast cancer and their clinical significance.Methods Expression of MSH2 and CyclinD1 proteins was detected in 67 cases of invasive ductal carcinoma of the breast by immunohistochemistry.Results The positive expression rate of MSH2 and CyclinD1 proteins was 53.7％ (36/67)and 47.8％ (32/67)respectively.The expression of MSH2 and CyclinD1 protein was closely related to lymph nodes metastasis(P ＜ 0.05).Expression of MSH2 and CyclinD1 protein was negatively correlated with each other in breast cancer(r =-0.251,P =0.040).Conclusion CyclinD1 may play a role in DNA mismatch repair(MMR) system by downregulating the expression of MSH2 and thus influences the tumorigenesis and progression of breast cancer.

Objective To analyze quality of life (QOL) and its related factors in community middle-aged and aged people with osteoporosis (OP). Methods A total of 152 OP patients in one community in Shanghai were surveyed and their QOL was evaluated with a questionnaire integrated with the QUALEFFO-41 questionnaire. Factors were analyzed using a Kruskal-Wallis test and stepwise multiple regression. Results The QOL scores of these middle-aged and aged people with OP were generally low (62.58 + 14.06). Age, education level, milk intake, a history of fractures or illness, OP grade and menopause time were significant factors predicting QOL scores. Stepwise multiple regression indicated that fractures, illness, education level and menostasis time had a linear relationship with QOL. Conclusion The key points in improving the QOL of middle-aged and aged people with OP in the community are health education, early diagnosis and comprehensive treatment.

Objective To investigate whether antisense integrin αV and β3 gene therapy has antitumor activity for hepatocellular carcinoma.Methods In this study,120 male nude mice at the age of 4 weeks old were devided into 4 groups randomly.The antisense integrin αV and β3 expression vectors were iniected into HepG2 hepatomas established in nude mice to monitor tumor growth after 6 weeks.Tumot inhibit rate was calculated.Tunor microvessel density(MVD)was determined by immunohistochemical staining,and tumor apoptosis by TUNEL. Results The tumor weight of the control group,αV group,β3 group and αVβ3 group was(1.38±0.92)g、(1.28±0.27)g、(1.30±0.34)g、(1.08±0.16)g respectively.The difference between each two groups was significant(q12=4.76,q13=3.73,q14=14.28,P＜0.05);The MVD was(17.53±1.88)、(16.06±1.92)、(15.83±2.00)、(14.86±1.69)respectively,(q12=3.91,q13=4.55,q14=7.13,P＜0.01);The AI of the control group,αV group,β3 group and αVβ3 group was 10.53%±3.29%,19.80%±4.06%,21.93%±3.26%,24.03%±4.45%respectively,with the difference being significant among groups(q12=29.41,q13=33.52,q14=39.7,P＜0.01).Conclusions Antisense gene therapy targeting αV and β3 integrins exerts dramatic inhibition on the growth of the tumor.