Objevtive To evaluate the efficacy and safety of intensive pulse light (IPL) on treating the telangiectasis and dermal atrophy due to corticosteroid-dependent dermatitis.Methods We applied the IPL on 13 patients who had telangiectasis and dermal atrophy caused by corticosteroid-dependent dermatitis every three to four weeks.The treatment lasted three to eight times.The patients's skin types were Fitzpatrick Ⅲ-V and they had had corticosteroid therapy from 15 months to 13 years.Results To telangiectasis,3 patients had excellent effect afterwards;7 patients achieved fair good effect;Another patients also achieved improvement;2 cases had no positive effect;The total effective rate reached 76.92%.10 patients of dermal atrophy among the total 13 had improvement of the thickness of dermis.Conclusion Intensive pulse light can improve the telangiectasis and dermal atrophy of corticosteroid-dependent dermatitis.It needs less recovery time with fewer side effects and therefore IPL is an effective method for the Iesions.

Synuclein-γ (SNCG) is overexpressed in many advanced stages cancers, including breast cancer, ovarian cancer, prostate cancer, lung cancer, liver cancer, esophagus cancer, and colon cancer.SNCG stimulates the proliferation, metastasis, and drug resistance of tumor cells, through interacting with BubR1 and damage mitotic checkpoint. A peptic ANK targeted at SNCG can inhibit activity of SNCG and may be developed as an adjuvant therapy.

AIM: To establish the quality standard for Compound Yiqi Granules (Radix Astragali, Radix Achyranthis Bidentatae, etc.). METHODS: Radix Achyranthis Bidentatae; Herba Epimedii were identified by TLC. The content of astragaloside IV in Compound Yiqi Granules was determined by TLCS. RESULTS: The methodological study showed a good linear correlation at the range of 1.12-5.60 ?g of astragaloside IV. The average recovery of astragaloside IV was 97.69% and RSD was 2.10%, respectively. CONCLUSION: The method is simple, accurate and with good repeatability. The method can be used for quality control of Compound Yiqi Granules.

Background Glucocorticoid drugs have been used increasingly in ophthalmology.It has been established that glucocorticoid are associated with a rise in intraocular pressure (IOP) and the development of glaucoma.Selective laser trabeculoplasty (SLT) is thought to be effective in treating patients with glucocorticoidinduced elevated IOP.Objective This study was to assess the efficacy of SLT in lowering IOP in patients with glucocorticoid-induced ocular hypertension.Methods A retrospective case series study was adopted.SLT around 360° chamber angle was performed in 9 eyes of 9 patients with glucocorticoid-induced glaucoma in Eye & ENT Hospital of Fudan University,including 5 eyes of 5 patients owing to use of glucocorticoid eye drops for long-term after laser in situ keratomileusis (LASIK) and 4 eyes of 4 patients who received intravitreal injection of 0.1 ml triamcinolone acetonide (TA) (4.0 mg) for macular edema induced by central retinal vein occlusion(CRVO).All of the patients were lack of preexisting glaucoma or ocular hypertension and underwent unsuccessful maximum tolerated medical therapy before SLT treatment.The base IOP was 35-44 mmHg and glucocorticoid drugs were ceased for 2-12 months prior to the SLT.The patients were followed-up for 6 months.IOP was measured and recorded before and 1 hour,1 week,1 month and 3 months,6 months after SLT.The difference of IOP was compared by repeated measures analysis of variance and multiple comparison analysis.Results All the patients received single SLT operation.The mean IOP was (40.0±2.9) mmHg before operation,but the IOP was (37.9±8.1),(34.9±5.9),(27.6±6.7),(21.6±6.9) and (17.9±2.9)mmHg 1 hour,1 week,1 month,3 months and 6 months after SLT.The IOP at 1 month,3 months and 6 months after SLT were significantly lower than that before operation (all at P＜0.05).Two patients received a filtration surgery for uncontrolled IOP at 1 month and 3 months after SLT respectively,and another patient still used 2 kinds of lowing-IOP eye drops until the end of following-up duration.Conclusions SLT reduce IOP in 6 eyes of 9 patients with glucocorticoid-induced increased IOP from the initial 1 month through 6 months after SLT.

Objectives:To investigate the experience in insertion and management of central venous catheter. Methods:132 cases received insertion of central venous catheter.The site of catheter tip was determined with the method of electrocardiograph.The insertion depth was calculated with method of Fujii.The catheter was managed with strictly sterile technique and its lumen was washed with 0.1 mol/L NaOH 2.0 ml. Results:All catheters were inserted smoothly and its tips lay in suitable sites.128 pieces of catheter were pulled out after finished infusion. Conclusions:A right method of insertion and management is in favor for the use of a central venous catheter.

Objective To study the correlation between ths DSA manifestations and expression of serum VEGF in the patients with cervical cancer.Methods Imaging data of 40 cases with cervical cancer confirmed by clinic were collected,and DSA were performed during interventional therapy in all cases,and the serum VEGF was detected by enzyme-linked immunosorbent assay(ELISA)which was compared with that in 40 healthy women as contrast.Results(1)Serum VEGF level in the patients with cervical cancer was significantly higher than that in control group(P＜0.01),and the serum VEGF level was significantly higher in Ⅲ and Ⅳ stage of cervical cancer than that in Ⅱ stage(P＜0.05).(2)There was positive correlation between tumor stain in DSA and the serum level of VEGF(P＜0.05).Conclusion There was positive correlation between the degree of blood supply and the serum level of VEGF.

Objective To explore the status quo of the career planning of the second child nursery under the two-child fertility policy, and to provide reference for carrying out the targeted management intervention. Method From April to May of 2017, 592 nurses of the second child were surveyed using the general data questionnaire and the career planning scale. Results The nurses' career planning score (34.81 ± 3.59) was different, and the career planning of the two nurses with different age, job title, title, establishment, department and income and fertility was different and statistically significant. Nurses with plan to a second child are with less desire to further study and promotion than those without plan to a second child (all P<0.001) promotion to open more than two children before the low policy (P <0.05). Conclusion Nurses' career planning for births is on a middle level. Nursing managers should pay attention to the management of nurses' career planning , create a career development environment , provide humanistic care , strengthen self-awareness and career self-development of nurses who have two births , so as to improve the level of their career planning to promote the nursing team development.

Objective To study the value and feasibility of temporary emergency bedside cardiac pacing. Method Two hundred patients with severe witnessed bradycardia were treated with temporary emergency cardiac pacing. We treated 130 patients with emergency bedside pacing and 70 patients with x-ray-guided pacing. Results Emergency bedside pacing was successful in 127 patients except three patients and no postoperative complications occurred. X-ray-guided pacing was successful in all 70 patients but three patients experienced complications: one deep venous thrombosis and two cardiac tamponades due to myocardial perforation. The pacing electrodes were more likely to be displaced in X-ray-guided pacing than in emergency bedside pacing (six cases versus three cases) . The door-to-operation time was 30-90 min for x-ray-guided cardiac pacing and 5-15 min for emergency bedside pacing. Needle-to-pacing times were similar for both procedures (3.5 ± 1.5 min for x-ray guided pacing versus 4± 2.5 min for bedside pacing). Conclusions Temporary emergency bedside cardiac pacing is a rapid, efficient and safe procedure for treating severe witnessed bradycardia. The technique is easily mastered and may prove lifesaving in an emergency.

Objective To investigate the clinical significance of IL-27 in hepatocellular carcinoma ( HCC) in the early stage , and to compare it with alpha-fetoprotein ( AFP) in diagnostic performance .Methods Enzyme-linked immunosorbent assay was used to detect the serum level of IL-27 in the HCC group and control group .Receiver operator characteristic ( ROC) curve was used to ana-lyze two indicators and the logistic regression model predicted value ( PRE) was obtained .Results The study indicated the concentra-tion of IL-27 in HCC group [(364.19 ±177.55)pg/ml] was significantly higher than the control group [(255.49 ±94.33)pg/ml], the area under the curve (AUC) of IL-27 and AFP were (0.804) and (0.818), respectively.Logistic regression obtained the regres-sion model PRE that contains AFP and IL-27, with a predicted area under the ROC curve for HCC (0.901), while the diagnostic sen-sitivity (84.8%) and specificity (96.7%) were significantly higher than the capability of individual diagnosis of each indicator .Con-clusions In this study we obtained the model which can be used for clinical diagnosis of hepatocellular carcinoma in future .

BACKGROUND: P75 neurotrophin receptor (P75NTR) has a bidirectional role in promoting and inhibiting bone formation in bone tissue. Overexpression of P75NTR can inhibit the osteogenic mineralization of bone marrow mesenchymal stem cells. P75NTR can inhibit the degradation of fibrin and the formation of new blood vessels around the fracture, leading to fracture nonunion. Its inhibition of bone tissue repair is related to its mediating apoptosis channels. OBJECTIVE: To study the effect of silencing P75NTR on the growth activity and alkaline phosphatase activity of bone marrow mesenchymal stem cells, and to study the ectopic osteogenesis ability implanted in demineralized bone matrix to construct tissue engineering bone complex. METHODS: Lentivirus-mediated silencing P75NTR was transfected into bone marrow mesenchymal stem cells. The expression of fluorescent protein and P75NTR protein was detected by fluorescence inverted phase contrast microscope and western blot assay. Two or three days after transfection, CCK-8 assay was used to detect cell activity. After osteogenic induction solution induced differentiation culture for 7 and 14 days, alkaline phosphatase activity was detected by enzyme labeling method. Lentivirus-mediated silencing P75NTR transfected bone marrow mesenchymal stem cells were cultured with compound demineralized bone matrix. Optical inverted phase contrast microscope and scanning electron microscope were used to observe the adhesion of demineralized bone matrix and bone marrow mesenchymal stem cells. After further osteogenic differentiation for 7 days, tissue engineered bone complex was subcutaneously implanted in the rat back for 4 weeks. The osteogenesis was observed by hematoxylin-eosin staining and alkaline phosphatase staining. RESULTS AND CONCLUSION: (1) Lentiviral-mediated silencing P75NTR transfection rate was approximately 70%. The expression of P75NTR target protein was significantly lower than that of untransfected group and negative virus control group (P < 0.05). (2) Compared with untransfected group and negative virus control group, the cell activity of silencing P75NTR transfected group increased significantly at 5 and 6 days (P < 0.05). Compared with the untransfected group and the negative virus control group, the alkaline phosphatase activity of silencing P75NTR transfected group increased significantly at 7 and 14 days after osteogenic induction (P < 0.05). (3) Silencing P75NTR transfected bone marrow mesenchymal stem cells and demineralized bone matrix formed a good adhesion; the tissue engineered bone complex significantly enhanced the ability of ectopic bone formation. (4) The results showed that bone marrow mesenchymal stem cells transfected with silencing P75NTR mediated by lentivirus could be implanted in decalcified bone matrix to form a good tissue engineered bone complex, which has a certain heterotopic osteogenesis effect and provides important theoretical basis for bone tissue engineering in the treatment of bone defects.