This article introduces the proteomic study of the cell lines,tissue,pancreatic juice and serum from well-diagnosed patients of pancreatic cancer,benign pancreatic disease,normal control,etc.The highly specific and sensitive biomarkers and their significant roles in the mechanism of development and progress of pancreatic cancer,early diagnosis,therapy monitoring,prognosis,follow-up,and so on,are presented and discussed.

Immune system is the autologous defence system to resist disease.αβ TCR+ CD3+ CD4-CD8-NK1.1-double negative T cell is a special T lymphocyte which has been described in both humans and rodent models.In recent years,the prognosis has been made in the mechanisms by which DN Treg target antigen-specific T cells and tumor cells.DN Treg cells play an important role in immune regulation,immunosuppression,autoimmune and resistance to the host reaction,HIV infection and antitumor.The study mainly introduces the advance of DN Treg cells in immune treatment.

Neural invasion is an important invasion pathway of pancreatic cancer.New research shows that neural invasion of pancreatic cancer related genes in the sequential role effect,through the cell signal transduction,regulation of specific growth factors,adhesion molecules,matrix metallopmteinase and other related systems,then changed in the generation,resulting in the cancer cells invasion of the nerve tissue eventually.We reviewed the progress of neural invasion of pancreatic cancer in this paper.

Pancreatic cancer is a highly malignant tumor of digestive tract which early diagnose is very difficult and has low rate of surgical resection of advanced pancreatic cancer. However, the rate of postoperative recurrence and metastas is high. Its prognosis is poor. To improve the prognosis of pancreatic cancer , it is necessary to improve its early diagnosis and effective prediction of postoperative recurrence and metastasis. In recent years, with the development of proteomics, the early diagnosis of pancreatic cancer and its early diagnosis of recurrence and metastasis was possible. Wsing proteomics technology for protein differences screening, isolation and identification is conductive to early detection of pancreatic proteome changes and establishment of the markers for early diagnosis, and recurrence and metastasis of pancreatic cancer.

Severe acute pancreatitis is a relatively common surgical disease. Patients have a hypermeta-bolic state and nutritional support for patients with SAP is needed. Early enteral nutrition support is a viable option and can improve the nutritional status of patients, protect the gut barrier function, reduce bacteria translocation to regulate the inflammatory response and reduce hospitalization costs. Combined with our do-mestic and foreign literatures in recent years, this review the feasibility and practical implementation of enter-al nutrition in severe acute pancreatitis treatment summarizs.

DNT cells(CD4-CD8-double-negative T cells)ale newly discovered immune cells,whose surface ex press neither CD4 nor CD8 molecules.In recent years,the prognosis and survival quality of tumour patient have been greatly improved through cell immunotherapy.Immunocyte can kill the tumour directly Or through immunoregu lation.The study of DNT cells(double-nnegative T cell)on cancer cells will be of vital significance.

The proteome is the entire complement of proteins expressed by a genome, cell, tissue or organism,which are all types of proteins expressed by one cell in the specific physiological or pathological states. The study of proteomics not only can help us to understand the general rules of activity of proteins,but also illuminate the pathologic mechanisms of numerous diseases. By proteomic analysis of normal individual and pathological individuals, we can find some disease-specific protein molecules, which not only can be a new target for drug design, but also can be a molecular markers of some diseases especially tumors.

In recent years,the prognosis and survival quality of tumour patient would be improved greatly through cell immunotherapy,which becomes the priority scientific research.Immunoeytes can kill the tumour cell directly or through immunoregulation.CD4CD8 double-negative T cells is a newly found subset of T cells whose SUlface express neither CD4 nor CD8.This article mainly introduces the advancement of one subet of DNT cell,DN Tregs,in tumor.

Immune system is the autologous defense system for people to resist disease. Immunotherapy stimulates the immune system to resist disease, including treatment with immunocyte and vaccine. In recent years, the prognosis and survival quality of tumour patients have been greatly improved through cell immunotherapy by immunoregulation and killing directly on tumor. Immunotherapy becomes the hotspot in research.The study of DN Treg cell on immunotherapy will be of vital significance.

Pancreatic cancer is one of the malignant tumor with a high case fatality rate.Because the disease onset symptoms is not obvious,and the disease develops quickly, the malignant degree is high, so it is difficult to do early diagnosis in clinical, leading to poor treatment effect.In recent years, more and more of the discovery and application of pancreatic tumor markers provide a new way for early diagnosis of pancreatic cancer.This article is a review of the current status of the research on the tumor markers associated with the early diagnosis of pancreatic cancer.