Objective To find a grading method for assisting in evaluating hypoxia degree of patients with pulmonary heart disease non-invasively. Methods To design a quantitative method to score degree of hypoxia of 67 patients with pulmonary heart disease by using acute physiology and chronic health evaluation Ⅱ (APACHE Ⅱ) as a frame and to make comparative analysis between the grading result and artery blood gas to find the correlation. Results There was a negative correlation between evaluation mark and pH、PaO2、SaO2 and a positive correlation between the evaluation mark and PaCO2, according to statistics. Conclusions Although the grading table of hypoxia evaluation can not substitute blood gas analysis, acting as a non-invasive method to assist in evaluating the hypoxia degree of patients with pulmonary heart disease, it can not only relieve patients' repeated punctuation pain, but also achieve the goal of estimating hypoxia.Consequently the non-invasive method to evaluate hypoxia is of clinical value.

Adolescent ; Child ; Child, Preschool ; Electroencephalography ; Female ; Humans ; Infant ; Infant, Newborn ; Male ; Status Epilepticus ; diagnosis ; drug therapy ; etiology

Isoelectric focusina Electrophoresis(lFE)was use to detenmine the isoelectric point of main protein composition in Chinese Traditional Medicine (TCM ) either of animal origin Such as (EuPotyPhage sinenses)orfrom fruit or seed of plants (such as the seed of CeIosia urgeatea). Results showed that their IFE bands werealways quite distinct,which can be used to assess their quality and to distinguish them from confusable varieties.

Objective To investigate the effects of CO 2 pneumoperitoneum during laparoscopic cholecystectomy (LC) on pulmonary functions in chronic pulmonary failure rabbits and their mechanisms. Methods A total of 50 healthy male rabbits ( oryctolagus cuniculus ) were randomly divided into 4 groups: normal control group (N 0: n=5, no pneumoperitoneum), experimental control group (T 0: n=5, no pneumoperitoneum), 10 mmHg experimental group (T 10 : n=20, 10 mmHg pneumoperitoneum) and 15 mmHg experimental group (T 15 : n=20, 15 mmHg pneumoperitoneum). After the successful establishment of emphysema rabbit models, CO 2 pneumoperitoneum was conducted and maintained for 2 hours at the pressure of 10 mmHg (1.33 kPa) and 15 mmHg (2.00 kPa), respectively. Pulmonary functions (total respiratory resistance, central resistance and total airway resistance) of the 4 groups were measured by pulse oscillation technique before and after the pneumoperitoneum, respectively. Results Total respiratory resistance, central resistance and total airway resistance increased after the establishment of emphysema models. And they decreased at the end of pneumoperitoneum compared with those before pneumoperitoneum ( q=17.824, P

Objective To explore the preoperative misdiagnosis in the breast cancer and the methods to improve the diagnostic accordance rate. Methods The preoperative diagnosis and results of 358 cases with breast cancer has been analysized retrospectively in the recent ten years. Results Total Cases with present diagnostic technique diagnosed befor operation was 176 only;the accordance rate was less than 50%.With aspiration biopsy and molybdenum palladium roentgenograph,the rate was 25%(79 cases).The actual preoperative accordance rate was less than 10%(32 cases).It is said approximately 90% cases were postoperatively diagnosed. Conclusion The present excision biopsy not only has made many patients lost the chance of chemotherapy preoperation, but also interferenced the application of new therapic methods in the clinical practice.Infrared spectroscopy and ultrasonography have been commonly used in the clinical practice.With the low diagnostic accordance rate,they were applied only for screening.With simplity and high diagnostic accor dance rate,aspiration biopsy and molybdenum pall adium roentgenograph should be enrolled extensively in the clinical practice. The puncture and specimen fixing technique also should be improved.

Objective To find an effective operational approach that can mostly reduce heat and other injury to Calot's triangle when separating and anatomizing it at LC operation.Method We abandoned traditional hot separating method by electric coagulation hook which can cause hot injury easily and cold separating method simply by elastic separating plier which can cause errhysis and illegibility of operational field.We applied sucting and scraping cold separating method mainly by suction apparatus which can keep operational field clear and avoid hot injury.Result 52 cases with different cholecyst disease (including cholecystolithiasis,cholecyst polyp,acute cholecystitis with edema and acute cholecystitis with partly gangrene) were adopted by this method and achieved satisfacted effect.Conclusion Sucting and scraping cold separating method are easily operated and can mostly keep separated tube skeletonized.It can avoid hot and other injury in operation,keep operational field clear by less errhysis.To acute cholecystitis with edema it has more advantage and are worth to adopting.

Objective:To explore the protective effect of biochanin A(Bioch A) on lipopolysaccharide(LPS) -induced dopaminergic neurodegeneration. Method:An in vitro model of LPS-induced dopaminergic neurodegeneration was used to investigate the protective effect of Bioch A on dopaminergic neurons against LPS-induced neurotoxicity by measuring [3H] DA uptake and counting TH-immunoreactive cells. Microglia were visualized by staining for the CR3 complement receptor with monoclonal antibody OX-42. The production of nitric oxide(NO) was determined by measuring the accumulated levels of nitrite in the culture supernatant with the Griess reagent,and release of tumor necrosis factor-?(TNF-?) was measured by immunosorbent assay. The production of superoxide was determined by measuring the superoxide dismutase(SOD) -inhibitable reduction of cytochrome C. Results:BiochA dose-dependently attenuated LPS-induced decrease in dopamine(DA) uptake and the number of dopaminergic neurons in rat mesencephalic neuron-glia cultures. BiochA also significantly inhibited LPS-induced activation of microglia and production of TNF-?,NO and superoxide in rat mesencephalic neuron-glia cultures and microglia-enriched cultures. Conclusion:Biochanin A may protect dopaminergic neurons from LPS-induced injury and its effectiveness in inhibiting microglia activation may underlie one of the mechanisms.

Objective To investigate the relation of DNA content and SPF to the clinicopathological characteristic in lung cancer.Methods Fresh specimens taken from 56 patients with lung cancer and 36 patients with non-malignant pulmonary lesions were measured for DNA index(DI),S-phase fraction(SPF) by using FACSCalibur 4200 flow cytometry.Results ① DNA index(DI) of lung cancer was 1.18?0.33,0.99?0.07 in lung cancer and non-malignant groups,respectively.The percentage of heteroploid was 78.6% in lung cancer,(5.6%) in non-malignant.DI and the positive rate of heteroploid were significantly higher in lung cancer groups than that in non-malignant group(P0.05);③ It was demonstrated that SPF was significant higher in lung cancer groups than that in non-malignant group. The SPF of heteroploid tumors was higher than that of diploid tumors(P

Up to present,the research of the early-warning system for the risk of the medical quality has been carried out both at home and abroad.The majority of these researches aimed at providing theoretical guidance for the scientific decision-making of local health departments.In order to construct quality of medical care evaluation indicators system.On the bases of intelligence research,drawing on a series of internationally recognized quality of medical care evaluation system.The majority of these researches aimed at providing theoretical guidance for the scientific decision-making of local health departments.However,the hospital-related research is very limited.Therefore,hospitals were selected as objects in this study.33 experts from 6 three"A" hospitals were selected.And by using a series of internationally recognized quality health care system as a source of reference and focusing on process control and standardizing of management,the early-warning system for the risk of the medical quality has been constructed based on the combination of theory and practice.At the same time,Delphi method was used to verify the results repeatedly to form a set of early-warning system for the risk of the medical quality which is objective,scientific and approved by experts.

Non-myeloablative allogeneic hematopoietic stem cell transplantation has been extensively applied in patients with hematologic malignancies who are ineligible for conventional hematopoietic stem cell transplantation because of age or medical comorbidities. Non-myeloablative regimens lead to an initial state of mixed hematopoietic chimerism which can produce a marked effect of graft versus tumor to treat the diseases. Compared with the conventional hematopoietic stem cell transplantation, non-myeloablative allogeneic hematopoietic stem cell transplantation has a lower transplant-related mortality and incidence rate of graft-versus-host disease. The improvement of non-myeloablative regimens and the prophylaxis of diseases associated with transplantation can improve the therapeutic efficacy. Though many therapies have been introduced and proved to be successful in animal models, we still need to investigate the research trend and the problem on human body.