OBJECTIVE:To prepare pazufloxacin mesilate ophthalmic gel and to establish its quality control method.METHODS:The gel was prepared with pazufloxacin mesilate ophthalmic as principal agent and with PVA-124 as base material.The content of pazufloxacin mesilate was determined by HPLC and the stability of the gel was investigated as well.RESULTS:The prepared gel was yellowish liquid and its property fitted the description of Chinese Pharmacopeia(2005 edition).The linear range of pazufloxacin mesilate was 20.0～80.0 ?g?mL-1(r=0.999 9),and the average recovery was 98.67%(RSD=0.79%,n=3).No stratification was noted for the sample within 2-month storage under room temperature.CONCLUSION:The preparation technique of the pazufloxacin mesilate ophthalmic gel is simple and feasible,its quality control method is rapid and the determination result is accurate and reliable.

OBJECTIVE:To prepare penciclovir hollow suppository and establish its quality control method.METHODS:Penciclovir hollow suppository was prepared by heat-melting method.The content of penciclovir was determined by HPLC and the stability of the suppository was investigated as well.RESULTS:The preparation was ivory yellow hollow suppository and its property fitted the description of Chinese Pharmacopeia(2005 edition).The linear range of penciclovir was 5.00～100.00 ?g?mL-1(r=0.999 7),and its average recovery was 98.69%(RSD=0.81%).No obvious change was noted for the property of the suppository within 1 year storage under temperature away from light.CONCLUSION:The preparation technique of the penciclovir hollow suppository is simple and feasible,its quality control method is rapid in operation and its determination result is accurate and reliable.

Objective To explore the curative effects of comprehensive care in the patients with hysteromyoma treated with UAE. Methods120 hysteromyoma patients were divided into 2 groups randomly.The observation group were treated with integrated nurse including psychology,cognition and praxiology.The control group were treated with conventional care. ResultsThe rates of VVR and low-grade fever in the observation group was lower than that of control group(P＜0.05).The spain in the observation group were less than that of control group(P＜0.01). ConclusionThe effective nursing intervention could reduce the complications after operations.

Objective To investigate the effects of different selective attention on the amplitudes of distortion product otoacoustic emission (DPOAE) .Methods DPOAE measurements were performed in 30 young adults (60 ears) in no task (baseline) ,visual selective attention and auditory selective attention ,respectively .The suppression of DPOAE amplitudes were observed in different selective attentions .The visual selective attention was to counter the number of letter Q presented on computer screen .The auditory selective attention was to counter the number of 2 .0 kHz toneburst in the insert earphone .Results Visual and auditory selective attention both decreased the DPOAE amplitudes in mid - low frequencies (0 .75 ~ 2 .0 kHz) .The suppression effects of visual selective attention were significant stronger than that of auditory attention in 0 .75 ~ 1 .0 kHz .The suppression amplitudes were 8 .54 ± 4 .76 and 5 .27 ± 2 .32 dB at 0 .75 kHz ,respectively .They were 7 .66 ± 5 .22 and 3 .22 ± 2 .15 dB at 1 .0 kHz ,re‐spectively .There were significant differences between the two selective attentions suppression (P< 0 .05) .Conclu‐sion Visual and auditory selective attention can both decrease the DPOAE amplitudes .The suppression effects of visual selective attention were significant stronger than that of auditory attention .

Objective To explore the short-term efficacy and safety of edaravone combined with clopidogrel in treatment of acute cerebral infarction (ACI).Methods 120 cases of ACI were selected and they were randomly divided into the observation group(60 cases)and the control group according to the visiting order(60 cases).On the basis of routine treatment of ACI,the control group were treated with clopidogrel,and the observation group were added with edaravone combined with clopidogrel.All patients were treated continuously for 14 days.Patients' neural function,activities of daily living,and adverse reactions were evaluated before and after treatment.All patients were followed up for 1 year to record recurrence.Results After treatment,patients' limb movements,neural functions and language had varying degrees of recovery.Total efficiency was 93.33％ and 78.33％ respectively for the observation group and the control group.Grading of the neurologic impairment and life ability of the observation group were both better than the control group.The one-year recurrence rate was 5.77 ％ and 9.26％ for the observation group and the control group,and the difference had statistical significance (P ＜ 0.05).No obvious adverse reaction in the 2 groups was found.Conclusion The curative effect of edaravone combined with clopidogrel is remarkable in treating ACI,and it can remit patients' neurological deficit,improve their daily life ability,reduce the recurrence rate,and has high safety.

To assure the quality and safety of nuclear medicine imaging,quality control for the imaging equipment is essential.Quality control consists of a series of special tests for assurance and adjustment of equipment accuracy according to specific standards.The development and status of quality control and the standards for nuclear medicine imaging equipment are investigated and reviewed in this study.The results show that the quality control and feasibility of the standards are both in need of improvement in China.

BACKGROUND:The research for mesenchymal stem cels in the treatment of liver cirrhosis has made great progress. However,t-test analysis is often misused in clinical practice. OBJECTIVE:To analyze the efficacy and safety of umbilical cord mesenchymal stem cel therapy for liver cirrhosis by repeated measurement method. METHODS: A total of 27 patients with decompensated liver cirrhosis underwent conventional medical treatments, including liver protection treatment and symptomatic treatment. At 1 week after hospitalization, patients were given intravenous transplantation of umbilical cord mesenchymal stem cels (passages 2-4), cel viability≥ 90%, stem cel counting≥ 2×107 cels, four times with an interval of 5-7 days. Analysis of variance based on repeated measurement data was performed to analyze the liver function changes at different time after umbilical cord mesenchymal stem cel transplantation. RESULTS AND CONCLUSION: The results of univariate repeated measurement showed that at 2 and 3 months after treatment, the serum albumin level was increased and the total bilirubin level in serum was decreased significantly (P < 0.05); at 3 months after treatment, the content of aspartate aminotransferase decreased, and the content of cholinesterase increased significantly (P < 0.05). No patient appeared to have liver and other organ tumors during the observation period. These findings indicate that umbilical cord mesenchymal stem cel transplantation is effective and safe in the treatment of liver cirrhosis, which can significantly improve patient's liver functions.

Objective To investigate the feasibility of transplanting bone marrow stromal cells (BMSCs) in the injured spinal cord for differentiation into functional oligodendrocytes in rats. Methods The ultra-structure of transplanted BMSCs in the injured rat spinal cord was observed at 1,3,5 weeks after transplantation by immuno-electron microscopy and low temperature embedding.The expressions of myelin basic protein (MBP) and proteolipid protein (PLP) in the transplanted BMSCs in the injured spinal cord were observed at 1,3,5 weeks after transplantation by immunofluorescence staining and laser confocal scanningmicroscopy. Results The organelles in the BMSCs migrated into the white matter of the spinal cord anddeveloped at 1 week after transplantation,showing the ultra-structure features of oligodendrocytes.The BMSCs migrating into the white matter of the spinal cord showed mature ultra-structure features of oligodendrocytes and formed myelin-like structures at 3 and 5 weeks after transplantation.The BMSCs migrating into the white matter of the spinal cord expressed the MBP and PLP at one week after transplantation,and continued to express the MBP and PLP at 3 and 5 weeks after transplantation. Conclusion The BMSCs transplanted into the injured spinal cord nay partly differentiate into functional oligodendrocytes.

ObjectiveLamiophlomis rotate is the rare perfumes Tibetan medicine.This article aimed to explore the current research of Lamiophlomis rotate on the base of literature.MethodsEstablish database of researching literature on Lamiophlomis rotate in domestic published between 2000 and 2010.Results Altogether 210 articles distributed in 112 kinds of journals were found.These articles involved with 8 areas of clinical research(113 article),pharmacological research(18 article),component analysis(35 article),preparation process (15 article),breed and cultivation of seeds (4 article),new drug testing (3 article),biological characteristics and drug economics.32 articles gained fimancial assistance from fund.Three articles on breed and cultivation of seeds gained financial assistance from fund,occupying 75.00％ of such research.Conclusion There were few articles published in core journals,besides relatively scattered distribution of researching institute and journals.Studies on breed and cultivation of seeds should be strengthened.

Objective To observe the effects of minimally traumatic puncture drainage of hematoma combined with Salviae Miltiorrhizae injection(丹参注射液) on the edematous volume around the lesion and neural functional defect in patients with hypertensive cerebral hemorrhage.Methods Eighty-nine patients with hypertensive cerebral hemorrhage were randomly divided into two groups: treatment group(n=45) and control group(n=44).All the patients had received minimally traumatic puncture drainage of hematoma,dehydration therapy,hemostatics,anti-inflammatory and anti-hypertensive agents, etc.In addition,Salviae Miltiorrhizae injection was continuously injected intravenously in the patients in the treatment group for three weeks.Cranial CT was performed to measure the volume of edema around the lesion at 7,14 and 21 days before and after operation,neurologic impairment degree scores(NDS) were assessed by National Institutes of Health Stroke Scale(NIHSS),and at the same time the fatality rate and re-hemorrhagic situation were observed at 14 and 30 days before and after initiating the operation.Results The areas of perilesional edema in both groups were conspicuously diminished at 7,14 and 21 days after initiating the operation,the area of perilesional edema diminished gradually,and significantly smaller in the therapeutic group than that in the control group at 14 and 21 days(P