Acupuncture Therapy ; methods ; Adult ; Aged ; Female ; Humans ; Intervertebral Disc Displacement ; therapy ; Male ; Middle Aged ; Young Adult

The diagnosis with unknown pathogens is an important cause of inappropriate use of antimicrobial agents. The microbiology laboratory should intensively cooperate with the clinicians to evaluate the quality of respiratory tract samples. The technicians of microbiology should strengthen the training for basic skills, such as direct smear and a variety of staining. Meanwhile, the laboratory should perform the rapid detection techniques to improve the diagnostic level of pulmonary infections.

BACKGROUND:Deep venous thrombosis is one of the common and fatal complications of total joint arthroplasty.For our clinical practice,we require duplex ultrasound and venography to evaluate the presence of deep venous thrombosis,but the diagnostic tools have several disadvantages.OBJECTIVE:To explore the appropriate and non-invasive biomarkers in the early diagnosis of deep venous thrombosis.METHODS:With the key words of Thrombosis,DVT,Biomarker,Total Joint Arthroplasty,D-dimer,Factor VIII,Thrombin Generation,Fibrin Monomer,P-selectin,Inflammatory Cytokines,Microparticles,Leukocyte Count,Genetic factors,MicroRNAs,a computer-based search was performed for articles published in PubMed database from January 1995 to March 2015.After the initial screening,the reserved articles were further detailed,summarized and concluded.RESULTS AND CONCLUSION: Totaly 66 articles were colected.This brief review is used to analyze the mechanism of the biomarkers which are classified into three levels as folow: detection of blood coagulation,inflammation markers and molecular biology and genetics-based biomarkers.The review also showed a great need of economic and safer biomarkers for the clinic and provided the theoretical basis to diagnose and predict deep venous thrombosis in an early stage.

Antimicrobials resistance can be detected by conventional susceptibility testing and genetic method. Most genetic methods are based on PCR, and can be used directly in clinical specimens to guide therapy early and rapidly.Great achievement has made in basic study of antimicrobial resistance genes in China.Applied studies in genetic method of resistance genes are needed.

Objective To investigate resistance mechanism against erythromycin in Streptococcus pneumoniae from Beijing region. Methods 116 strains of erythromycin resistant Streptococcus pneumoniae were collected from 1998 to 1999 at Peking Union Medical College Hospital Serotyping was done with "capsular swelling" technique erm/mef genes were detected with PCR, pulsed field gel electrophoresis (PFGE) and penicillin binding protein (PBP) fingerprinting technique were used to detect the DNA of resistant strains Results The prevalent serotypes in the 116 erythromycin resistant strains were 23F(30 0%),6A(19 0%),19F(13 8%),15(7 8%),23A(5 2%) 95 7% of penicillin resistant strains were also macrolide resistant, most (85%) expressing the MLS phenotype with co resistance to clindamycin The macrolide resistance determinant in 86 4% of erythromycin resistant strains was the erm gene, both the erm and mef genes were found in 6%, mef alone in 1 7% and no mechanism in 4 2% PFGE identified two clones: one a serotype 23F clone resistant to penicillin; and the other a penicillin susceptible and macrolide resistant serotype 6A clone Conclusions Ribosomal modification (erm gene coded) was the main resistance mechanism against erythromycin in Streptococcus pneumoniae in Beijing region Two resistance clones bear concern

OBJECTIVE:To determine the concentration of Dermatan Sulfate,a new antithrombotic medicine in blood or urine by spectrophotometry.METHODS:In Britton Robinson buffer solution(pH=5.8),color fading reaction occurred when Dermatan sulfate combined with neutral red dye to form ionic associate.The reduction of absorbance of the system was positively correlated to dermatan sulfate concentration,with the maximum absorption wavelength at 526 nm.RESULTS:The linear range of dermatan sulfate was 0.18～4.0?g?mL~(-1)(r=0.999 1) with a detection limit of 0.054?g?m~(-1).The average recovery was 100.3%(RSD=1.2%),with RSD of dermatan sulfate at low,medium,and high concentrations at 2.9%, 1.5%,and 1.1%,respectively.The coexisting substances did no interference on the determination results.CONCLUSION: The method is simple,accurate,and sensitive with good methodological selectivity and it achieved satisfactory results in the determination of dermatan sulfate in blood or urine.

In this paper, the general conditions in research of dermato to xicology was described. The some experimental methods were evaluated. The advanc ement of modern sciences will accelerate the development of dermatotoxicology an d make more progresses and new ideas.

AIM: To observe the effect of sibutramine in the treatment of obesity with different body weight. METHODS: Forty overweight subjects were divided into three groups according to their BMI: Group 1 (n=10), the early stage of obesity; Group 2 (n=11), obesity in degree Ⅰ; and Group 3 (n=19), obesity in degree Ⅱ. The patients conformed to the same standards of diet, sports and life style, and then took sibutramine 10 mg a day for 12 weeks. RESULTS: After taking the medicine for two weeks, the efficacy rate was 45.5 % (5) in Group 2 and 94.7 % (18) in Group 3 (P

Objective To observe the influence of Zhibitai capsule combined with atorvastatin calcium tablet on blood lipid and inflammatory factors in elder patients with dyslipidemia. Methods Sixty-four elder patients with dyslipidemia were randomly divided into control group and treatment group, with 32 cases in each group. Patients of the control group were treated with atorvastatin calcium tablet (10 mg, qd, bedtime administration), while patients in the treatment group were given Zhibitai capsule (240 mg, bid) for 6 weeks. Serum levels of TC, TG, HDL-C, LDL-C, tumor necrosis factor-alpha ( TNF-α) and interleukin-6 (IL-6) were measured before and after treatment. Results After treatment, compared with the control group, TC [(5. 18±0. 57) mmol·L-1 vs. (5. 73±0. 65)mmol·L-1], TG [(1. 52±0. 43) mmol·L-1 vs. (1. 86±0. 48) mmol·L-1], LDL-C [(3. 36±0. 48) mmol·L-1 vs. (3. 85±0. 53)mmol·L-1], TNF-α[(5. 37±2. 21) ng·L-1 vs. (7. 63±2. 59) ng·L-1] and IL-6 [(12.27±2.75) ng·L-1 vs. (15. 63±2. 92) ng·L-1] were significantly decreased (P<0. 01 for all); however, HDL-C [(1. 26±0. 25) mmol·L-1 vs. (1. 13±0. 23) mmol·L-1] was significantly increased in the patients of treatment group (P<0. 05). Conclusion Zhibitai capsule combined with atorvastatin calcium tablet may not only regulate blood lipid level, but also decrease serum tumor necrosis factor-alpha and interleukin-6 so as to inhibit inflammation reaction in elder patients with dyslipidemia.

Many specific therapeutic antibody drugs have been developed for different indications. In drug development, it has been found that the antibody isotype framework can not only affect the physical and chemical properties of therapeutic antibodies, but also influence the activity and therapeutic effect. As a result, IgG isotype selection should be considered carefully in antibody drug development strategies. Because of the unique biological characteristics, IgG4 isotype has been used in some therapeutic antibodies for which effector functions are not desired. In order to provide new ideas for the development of antibody drugs, the research and application progress of IgG4 isotype in therapeutic antibody drug development has been reviewed.
Drug Design ; Humans ; Immunoglobulin G ; chemistry ; pharmacology