Objective: To observe the stability of tinidaz ole mixed with cefradin in glucose injection under room temperature or 37 ℃ for 8 hours.　Methods:Concentrations of tinidazole and cefrad in were determined by ultraviolet spectrophotometry and pH by a pH meter.　Results: The concentrations of tinidazole and cefradin and the pH of the solution did not change significantly under room temperature and 37 ℃ for 8 hours.　Conclusions: Tinidazole can be mixed with c efradin in glucose injection for clinical use under room temperature or 37 ℃.

Japan’s long-term care insurance takes grassroots governments as its insurer and the elderly and part of the non-elderly as its insured;The costs is shared by government、society和individual, and is, payed through isti-tutions, communities and hothes. Since its establishment, its security level has been increasing, the role of preven-tion has been rising, the status of community-based and home-based has been highlighting, and the cooperation a-mong relevant industry has been strengthening. But at the same time, its costs was soaring during the past decade, and the continued development of the system is facing transformation pressure now. Take this as a warning, the devel-opment of long-term care in China should learn its strong points, but also try to avoid its shortcomings.

An analytical method of reversed phase high performance liquid chromatography (RP-HPLC) was developed for simultaneous determination of 5-hydroxymethylfurfural and nine phenolic compounds (including (+)-catechin, (-)-epicatechin, chlorogenic acid, rutin, caffeic acid, protocatechuic acid, syringic acid, ferulic acid and p-coumaric acid) in 20 min.Ten components were detected and separated successfully by Diamonsil C_(18) column (150 mm x4.6 mm, 5 μm) at wavelength of 280 nm and column temperature of 42 ℃, with acetonitrile and 3% acetic acid solution as the mobile phase in gradient elution.The resultant correlation coefficients of the ten compounds were between 0.9911 and 0.9995 with detection limit from 0.2 to 0.5 mg/L, the RSD less than 2.4% and the average recoveries from 89.4% to 98.3%.These experimental results demonstrate that 5-hydroxymethylfurfural and the nine phenolic compounds in different rice wine samples can be determined with the new method for practical uses.

Objective To investigate the clinical effect of laparoscopic radical hysterectomy in the treatment of early-stage endometrial cancer.Methods A total of 60 consecutive patients preoperatively diagnosed as having stage Ⅰ or Ⅱ endometrial cancer from August 1998 to December 2004 was divided into two groups,receiving either laparoscopic(Laparoscopic Group) or open radical hysterectomy(Open Group).According to the involvement of the muscular layer,whether or not a pelvic lymphadenectomy was required was determined.Perioperative parameters and survival time were prospectively studied.Results As compared with the Open Group,the Laparoscopic Group presented a longer operation time(247.8?77.8 min vs 196.6?63.7 min;t=2.789,P=0.007),similar blood loss(265.0?187.6 ml vs 350.0?210.9 ml;t=-1.649,P=0.104),similar number of resected lymph nodes(22.0?5.0 vs 22.8?5.2;t=-0.607,P=0.546),insignificant difference in complication rate (10/30 vs 15/30;?2=1.714,P=0.190),earlier time to first passing flatus(44.3?11.1 min vs 55.2?12.8 min;t=-3.524,P=0.000),and shorter hospital stay(8.7?3.2 d vs 10.2?2.0 d;t=-2.177,P=0.034).The patients in the Laparoscopic Group were followed for 28.6?17.4 months with tumor-free survival.In the Open Group,a follow-up for 28.9?16.6 months found 3 deaths form metastasis or recurrence.Conclusions Laparoscopic radical hysterectomy and pelvic lymphadenectomy may be an alternative for early-stage endometrial carcinoma.

OBJECTIVE To study the clinical distribution and the drug resistance of extended-spectrum ?-lactamases (ESBLs) producing Klebsiella pneumoniae and Escherichia coli. METHODS The clinical isolates were identified by VITEK-60 of Bio-Merieux of France. K-B agar diffusion method and confirmed test were used to detect the drug resistance of ESBLs-producing K. pneumoniae and E. coli. RESULTS The incidence of ESBLs was 52.8% in E. coli and 63.5% in K. pneumoniae. E. coli was mostly isolated from urine (22.1%). The second was isolated from pus/wound (11.7%). Majority strains were isolated from surgical and pediatric wards. K. pneumoniae was mostly isolated from sputum and throat swab (37.8%), 21.8% from neonate ward and 16% from child ICU. CONCLUSIONS To cure infections caused by ESBLs-producing K. pneumoniae and E. coli, imipenem is the best choice and piperacillin/tazobactam is the second choice.

OBJECTIVE To study the incidence of ?-lactamases and oxacillin-resistant Staphylococcus aureus and their drug resistance in our hospital. METHODS The clinical isolates were identified by VITEK-60 of Bio-Merieux of France. K-B agar diffusion method and confirmed test were used to detect the drug resistance of oxacillin-resistant S. aureus. RESULTS Among 175 S. aureus isolates, the incidence of ?-lactamases was 94.3% and the incidence of oxacillin-resistant S. aureus was 28.6%. Oxacillin-resistant S. aureus was mostly isolated from pus/wound (52.0%), then from sputum and throat swab (32.0%). CONCLUSIONS To cure infections caused by ?-lactamases-noproducing S. aureus, penicillin is the best choice. For infections caused by ?-lactamases-producing but oxacillin sensitive S. aureus, the first generation cephalosporins are the best choice, while curing infections caused by ?-lactamases-producing and oxacillin- resistanct, S. aureus nitrofurantoin and linezolid are the best choice. The resistance rate to vancomycin is zero. It should be carefully used in therapy.

Objective To develop an in vitro culture system for Cryptosporidium parvum in Madin-Darby canine kidney(MDCK) cell and observe its life cycle(from desquamate to oocyst).Methods Oocysts of C.parvum were co-cultured with MDCK cells in vitro.Culture condition was optimized and the life cycle of C.parvum investigated.Results The optimal culture conditions for C.parvum in MDCK cells were 2.0?105 cells cultured for 12 h, and infected by 1.0?105 oocysts in the Dulbecco′s Modified Eagle Medium with 5% FBS.Following 72 h co-culture, desquamate, sporozoites, trophozoites, meronts, microgametocytes, macrogametocytes, zygote, thin-wall oocyst, and thick-wall oocyst appeared orderly.Between the 60th and 72th hour, many oocysts emerged.Inoculated by the C.parvum-infected cell culture supernatant at the 48th hour, the immunosuppressed mice became infected.Conclusion The culture system provides a model for propagation of the parasites and demonstrates a complete in vitro life cycle of C.parvum.

Chemotherapeutic drugs conventionally used for their cytotoxic activity can also exert their influence on the immune system by grooming the tumor microenvironment,abrogating immune tolerance and inciting immune reconstitution.Due to their immunomodulatory features,chemotherapeutics,used as a preconditioning regimen and combined with subsequent immunotherapy,can mobilize the immune system to generate antitumor immune response.The synergy between chemotherapy and immunotherapy has brought some enlightenment to the development of new protocols for cancer treatment.

Objective: To observe effect of salvia miltiorrhiza and ligustrazine injection on patients with acute episode of chronic pulmonary heart disease (CPHD) complicated pulmonary artery hypertension. Methods: A total of 40 CPHD patients with pulmonary artery hypertension were randomly divided into routine treatment group (n=20, received routine treatment) and observation group (n=20, received salvia miltiorrhiza and ligustrazine based on routine treatment). Another 20 healthy subjects were regard as healthy control group. The pulmonary artery pressure (PAP), partial pressure of oxygen in artery (PaO2) , left ventricular ejection fraction (LVEF) and levels of C-reactive protein (CRP) and endothelin-1 (ET-1) were examined and analyzed in all groups before and after treatment. Results: After treatment, compared with routine treatment group, there were significant increase in total effective rate (75% vs. 90%) and PaO2 [（83.87±14.53）mmHg vs.（92.95±13.54）mmHg], and significant decrease in mean PAP [mPAP, （55.43±9.65）mmHg vs.（45.52±8.89）mmHg], CRP [(6.37±2.12) mg/L vs. (3.29±0.84) mg/L] and ET-1 [(52.37±20.79) ng/L vs. (40.29±16.04) ng/L] in observation group (P<0.05~0.01). There was no significant difference in LVEF between the two groups after treatment (P>0.05). Conclusion: For patients with acute episode of chronic pulmonary heart disease complicated pulmonary artery hypertension salvia miltiorrhiza and ligustrazine injection can significantly improve clinical effects，right heart function, decrease mean pulmonary artery pressure, improve endothelial function and inhibit inflammatory reactions.

In order to improve nursing informationization,and to promote the development of nursing informatics, our nursing department set up a new position called informatics nurse and a series of working systems and man-agement methods were developed. After implementation,the informatics nurse had achieved various goals,including:playing a role in developing 60 nursing information system programs,improving 11 medical and nursing process modification,collecting 399 items of nursing information system problems with 338 items solved,completing 22 nurs-ing information system using instructions,and organizing training meetings and lectures in the hospital for 12 times. The informatics nurse has been appreciated by medical staff and IT engineers who was believed to have promoted the development of nursing informatics in our hospital.