ObjectiveTo systematically review and evaluate the predictive factors of interstitial lung disease(ILD) in patients with polymyositis and dermatomyositis (PM/DM), and provide evidence for early clinical diagnosis. MethodsThe predictive factors of PM/DM-related ILD from 23 published papers were analyzed by Meta-analysis. Analysis of heterogeneity and sensitivity were performed and pooled odds ratio (OR) with 95％ confidence interval(95％CI) were calculated using either fixed or random effects models by Rev-Man 4.2 software. Stata 10.0 software was used to identify publication bias. ResultsMeta-analysis was performed to analyze seven factors including anti-Jo-1 antibody, fever, arthritis/arthralgia, Gottron's sign,antinuclear antibody(ANA), and dysphagia Raynaud's phenomenon. Factors correlated with ILD in patients with PM/DM and their OR values and 95％CI were as follows: the presence of anti-Jo-1 antibody 6.94 (4.74～10.16), fever 4.90(3.82～6.29), arthritis/arthralgia 3.93(3.21～4.80), Gottron's sign 2.52(1.24～5.14), and the presence of ANA 1.59( 1.02～2.47 ). Raynaud's phenomenon and dysphagia were not correlated with ILD in patients with PM/DM with OR values and 95％CI as 1.40 (0.97～2.01), and 1.21(0.94～1.56) respectively.ConclusionThe presence of anti-Jo-1 antibody, fever, arthritis/arthralgia, Gottron's sign and ANA are major predictive factors for ILD in patients with PM/DM.

This paper analyzed the importance of improving the humanistic quality of the students in medical university and explored the way to integrate humanistic spirit education into clinical microbiology course.

BACKGROUND: Early mammalian embryonic development and implantation is a very complex process, which is regulated by a variety of cytokines. Moreover, epidermal growth factor, transforming growth factor, leukemia inhibitory factor, insulin-like growth factor and vascular endothelial growth factor play an important role in this regulation process.
OBJECTIVE: To introduce cytokines related to early embryonic development, including types, structural features and molecular mechanisms, and meanwhile to review the effects of epidermal growth factor, transforming growth factor, leukemia inhibitory factor, insulin-like growth factor and vascular endothelial growth factor in the early embryonic development.
METHODS:A literature retrieval was performed to search relevant articles published from 1989 to 2015 using the keywords of “embryos development, epidermal growth factor, transforming growth factor, leukemia inhibitory factor, insulin-like growth factor, vascular endothelial growth factor”. Active effects of these five kinds of cytokines in overcoming the mammalian embryonic development block were reviewed and summarized to provide theoretical and experimental basis for further optimization of the embryonic culture system in vitro.
RESULTS AND CONCLUSION: Cel growth factors involved in embryonic development is the focus of the study. Insulin-like growth factor is capable of promoting the embryonic growth; epidermal growth factor exhibits multiform functions in the embryonic development; leukemia inhibitory factor directly affects oocyte cel development, and progesterone increases the expression of leukemia inhibitory factor to provide a good cytokine microenvironment for embryonic development; vascular endothelial growth factor promotesin vitro oocyte maturation, and plays a very important role in various stages of embryonic development. To clarify these regulatory patterns of cytokines cannot only contribute toin vitro embryonic culture, but also reduce embryonic apoptosis, which is of great significance for promoting embryonic development.

BACKGROUND:The successful establishment of human embryonic stem cel lines in vitro is of great significance to human embryonic development mechanism and developmental biology, cel and tissue transplantation in the treatment of certain diseases. OBJECTIVE:To summarize the progress of in vitro culture of embryos and establishment of embryonic stem cel lines, to explore the influential factors for in vitro culture of embryos, and the methods of culturing human discarded embryos, isolating inner cel mass and establishing embryonic stem cel lines, as wel as the establishing conditions for embryonic stem cel lines. METHODS:With the key words of“embryo, embryonic stem cel s, coculture, sequential culture”, the first author searched CNKI and SCI databases for literatures concerning in vitro culture and transplantation of embryos and establishment of embryonic stem cel lines published from 2000 to 2014. Systematic evaluation was conducted. Final y, 58 literatures were retained for result analysis. RESULTS AND CONCLUSION:The culturing condition for embryos in vitro is the key factor affecting embryo transfer outcomes, including culture medium component and culture system. In previous studies, the component and application of culture medium have changed greatly, and the culture system has altered from single culture to coculture and sequential culture. Ethical issues and embryonic origin restrictions restrict the establishment of human embryonic stem cel lines. Clinical y discarded low-quality embryos can be used as one of the material sources to establish human embryonic stem cel lines, which can effectively lessen the problem of embryo shortage during the establishment of human embryonic stem cel lines and reduce ethical disputes.

Objective To analyze the clinical risk factors of the occurrence of severe proliferative vitreoretinopathy (PVR) after scleral reattachment surgery. Methods A total of 4 031 eyes of 4 031 consecutive patients with reghmatogenous retinal detachment (RRD) and PVR (grade C1 or less), on whom the scleral buckling was performed, were retrospectively studied. Twenty-two clinical characteristics of the patients (including the ocular tension, condition of lens and vitreous, characteristics of retinal detachment, whether or not with choroidal detachment, et al) were recorded. In 4 031 patients, 2 660 were followed up for more than 3 months, and 72 (in PVR group) of the 2 660 patients underwent the second surgery (vitreoretinal surgery) because of the occurrence of postoperative severe PVR; in the other 2 588patients, 72 (72 eyes) with retinal reattachment for more than 3 months were selected randomly as the control. The data were analyzed in SPSS (10.0) software. Results Logistic regression analysis revealed that the significant risk factors for PVR were incomplete posterior vitreous detachment (P2 DD,P

Objective To observe and compare the regulating effect between electroacupuncture and sham electroacupuncture on the symptoms of menopausal transition and levels of estradiol (E2), follicle-stimulating hormone (FSH), and luteinizing hormone (LH), for evaluating the clinical efficacy of electroacupuncture in mitigating the relevant symptoms of menopausal transition. Method Thirty eligible subjects with menopausal transition were randomized into a treatment group and a control group, 15 cases in each group. The treatment group was intervened by electroacupuncture at the ordinary acupoints, while the control group was by electroacupuncture at non-meridian points. The E2, FSH, and LH contents were detected after intervention and at the 20th week during the follow-up study, the flush score and Menopause Rating Scale (MRS) were evaluated respectively after 4-week and 8-week treatment and at the 20th week and 32nd week during the follow-up study, and the safety of electroacupuncture was also estimated.Result The flush and MRS scores were significantly changed after 4-week and 8-week treatment and at the 20th week and 32nd week during the follow-up study in both groups compared to that before the treatment in the same group (P<0.01,P<0.05). There was a significant difference in comparing the changes of MRS score after 8-week treatment between the two groups (P<0.01). There were significant differences in comparing the changes of flushing scores between the two groups after 8-week treatment and at the 20th week and 32nd week during the follow-up study (P<0.01). The serum levels of E2, FSH, and LH were significantly changed in the treatment group after 8-week treatment (P<0.05). There were significant differences in comparing the changes of FSH and LH levels between the two groups after 8-week treatment (P<0.05). The difference in comparing the change of serum E2 level between the two groups at the 20th week during the follow-up study was statistically significant (P<0.05). Conclusion Electroacupuncture can improve the flushing intensity and produce a benign regulation on the relevant hormone levels in menopausal transition, though this regulation is insignificant when the treatment terminates.

Objective To analysis the clinical characteristics, treatment and prognosis for patients with cervical cancer with lymph lode of paraaortic and /or supraclavicular metastasis after combined treatment in two years. Methods Data of 20 patients with lymph lode of paraaortic and /or supraclavicular metastasis of cervical cancer were analyzed retrospectively. Results There was no evidence of lymph node of paraaortic and supraclavicular metastasis before the first combined treatments according to the MRI/CT/SPECT scans in these 20 patients. However, lymph lode of paraaortic and/or supraclavicular metastasis were seen in two years after treatments, the shortest is two months. One patient was progressing during treatment whose pathologic diagnosis is mucinous adenocarcinoma, died in 6 months from treatment start. There was only paraaortic metastasis in 15 patients, among these, 4 patients were found lymph lode of left supraclavical metastasis after retreatments in 3-6 months. Both paraaortic and left-supraclavical lymph node metastasis in 4 cases. Among these 20 cases, recurrence and to give up treatment, whose median survival time is 7.50 months, recurrence and to gain treatment, whose median survival time is 18.98 months, there was significant difference (x2=7.23, P<0.01). Conclusion Patients with cervical cancer with lymph lode of paraaortic and/or supraclavicular metastasis after combined treatment will obviously extend survival time after retretments, which has positive therapy significance.

Objective To observe the effeet of cotransduction of wt-p53,B7-1 and GM-CSF genes mediated by recombinant adenovirus on cell growth, apoptosis and immunogenicity of neuroblastoma cell line SH-SY5Y, Methods We transfected SH-SYSY cells separately with recombinant adenovirus, recombinant adenovirus mediating human wild type p53(wt-p53) gene and BB-102.We separately named them with SH/Ad,SH/p53 and SH/BB-102 group.The protein expression of p53, B7-1 and GM-CSF were measured by Western blot, FCM and enzyme linked immunosorbent assay. Cell number was counted and growth curves were drawed. Cell apoptosis was tested by FCM. Lymphocyte proliferation and cytokine secretory was evaluated by mixed lymphocyte culturing. Results After transection with BB- 102, p53, B7-1, and GM-CSF, high-efficiency expression of target genes were found. Growth of the cells was inhibited, apoptosis was induced in the SH/p53 and SH/BB-102 groups. Proliferation of lymphocytes was stimulated and cytokine increased visorously in the SH/BB-102 group. Condusions After transfection with BB-102, growth of the SH-SY5Y cells was inhibited,apoptosis was induced,and immunogenicity was greatly enhanced.

Objective To study the effect of berberine on the lipid metabolism and the expression of ox-LDL,CD36 and PPAR? in the peritoneal macrophage(PM) and alveolar macrophage(AM) from the type 2 diabetic rats. Methods Sprague-Dauley rats were randomly divided into groups of normal control,high-fat diet,type 2 diabetes and berberine treatment.The levels of blood glucose,insulin and lipid were measured biochemically,the content of ox-LDL in macrophage and the protein expression of CD36 and PPAR? in macrophage were assayed by ELISA,the mRNA expression of CD36 and PPAR? in macrophage was disclosed by RT-PCR. Results Compared with the control group, the levels of glucose,insulin and total cholesterol (TC),triglycerides(TG),low density lipoprotein-cholesterol (LDL-C) in blood were increased significantly(P0.05). The content of ox-LDL,the protein and mRNA expressions of CD36 and PPAR? in PM and AM increased in type 2 diabetic group(P

Objective To develop a pathology information system and provide a pathology testing platform with digitalization,normalization and optimization. Methods Based on the principal and requirement of information engineering theory to analyze the profession,flow-sheet management and data needs of pathology information system. The pathology examination and related testing were analyzed mainly,included some special requirements in installation of work position,quality control,cost management,which were transformed conceptual and logical structure of database and realized physical composition on Oracle database. The reciprocal software codes were researched by PB program,which can build database in servers and install application software in front end computer. Results The system that was specified the components of the clinical data entry,pathology examination registry,clinical document repository,EMR application server and the e-node accessing server. The system was tested and the result indicated that the system was an effective and scalable. Conclusion The pathology information system can be not only used realize the sharing of EMR documents across multiple hospital,but also support the collection clinical data within hospital clinical system integration.