Objective:Toclone the OPGgene ofhuman beingand construct its recombinant adenovirus and then observe its effects on the differentiation of bone marrow stem cells(BMSCs).Methods:The cDNAs of human OPG in hepatic tissue were obtained by using RT-PCR method.The cDNAs then were recombined with competent homologous cells to form the AdOPG and the shuttle vector was used during this stage.The rabbit BMSCs were isolated and cultured in vitro,and were infected with AdOPG.The OPG expression of BMSCs was identified by RT-PCR and Western blot.The expression of ALP was detected by chromatometry and ALP dyeing five days after the infection.Results:The OPG gene of human being was successfully cloned and its sequence consistent with the Gene bank.The titre of constructed AdOPG can reach 109 efu/ml.The ALP activity of BMSCs was(21 024?507)IU five days after the infection of AdOPG,whereas the value was(3 079?89)IU in the control group and(2 156?78)IU in the blank control group.Conclusion:The OPG can induce the osetogeinc differentiation of BMSCs.

Biorefinery that utilizes renewable biomass for production of fuels, chemicals and bio-materials has become more and more important in chemical industry. Recently, steam explosion technology, acid and alkali treatment are the main biorefinery treatment technologies. Meanwhile, low temperature plasma technology has attracted extensive attention in biomass refining process due to its unique chemical activity and high energy. We systemically summarize the research progress of low temperature plasma technology for pretreatment, sugar platflow, selective modification, liquefaction and gasification in biomass refinery. Moreover, the mechanism of low temperature plasma in biorefinery and its further development were also discussed.
Biomass ; Biotechnology ; methods ; Biotransformation ; Chemical Industry ; Cold Temperature ; Plasma Gases

Gene Expression Profiling ; Humans ; Laser Therapy ; Lung Neoplasms ; genetics ; Microdissection ; methods ; RNA, Neoplasm ; isolation & purification

Objective To explore the application of cognitive intervention and drug intervention in patients with chronic obstructive pulmonary disease(COPD).Methods A total of 86 patients with chronic obstructive pulmonary disease(COPD)admitted from February 2016 to July 2016 were included in this study.The patients were divided into experimental group and control group according to the order of admission.43 patients in the control group received routine measures,In the experimental group,43 patients received cognitive intervention and drug intervention,compared the clinical treatment effect and treatment compliance of the two groups after intervention.Results The compliance rate of disease cognition,smoking,medication,pulmonary function,family oxygen therapy,respiration exercise,rehabilitation therapy,nutritional support and influenza vaccination were significantly better in the experimental group than in the control group(P<0.05)(1.2±0.7)times,the number of acute attack was(2.0±0.8)times and the quality of life was(4.4±0.9)points,which was significantly better than that of the control group(P<0.05).Conclusion Cognitive intervention and drug intervention have a positive effect on the treatment compliance of patients with chronic obstructive pulmonary disease,improve the quality of life of patients,and have significant clinical application value.

BACKGROUND:Anterior cruciate ligament (ACL) reconstruction is the primary treatment method for ACL rupture. Currently, studies on ACL reconstruction involve histology and embryology, anatomical structure, biomechanics, reconstruction materials, operating technology, and rehabilitation after reconstruction. However, clinical epidemiological studies describing ACL rupture and reconstruction remain scarce. OBJECTIVE:To analyze the clinical epidemiological characteristics of ACL rupture and reconstruction to provide guidance for prevention and treatment of ACL rupture. METHODS:Data of 352 patients forage, gender, cause and mechanism of injury,treatment time, and the impact of ACL rupture on menisci and articular cartilage were gathered. Meanwhile, the events during surgery, operation methods and reconstruction materials were analyzed. RESULTS AND CONCLUSION:ACL rupture mostly occurred in young men,andhappened more often to theleft knee; male patients got hurt in basketbal, footbal and accidental injuries,whilefemale patients got hurt in the accidental injuries, badminton and sking injuries, internal rotation with valgus stress accounted for the predominant injury mechanism. The ACL reconstruction wasmostlyperformedwithin1-3 months after ACL rupture, often accompanied by meniscal and articular cartilage damage. Lateral meniscus injury incidence was relatively stable, medial meniscusinjury incidence increased significantly over the half year after ACL rupture. Most articular cartilage injury occurred to patelar cartilage.Asignificant increase in medial condylar cartilage damage over 1 year after ACL rupturewas often observed. Anatomic single-bundle ACL reconstruction was the primary surgical approach, the resident ridge and the lateral bifurcate ridge could be used to position bone tunnel and autogenous semitendinosus and gracilis tendon were the most commonly used reconstructionmaterials. Our results indicate that anatomic ACL reconstruction should be performed as early as possibleinrestore knee joint stability and prevent secondary injury of the medial meniscus and cartilage of medial femoral condyle.

Objective To observe the expression of intercellular adhesion molecule 1(ICAM-1)and vascular cellular adhesion molecuh 1(VCAM-1)in placenta in patients with hypertensive disorders in pregnancy,investigate their roles in patients with hypertensive disorders in pregnancy and correlation.Methods Fifty-two patients with hypertensive disorders in pregnancy(disease group)were collected,including 11 csses with gestational hypertension(GH),18 cases with mild pre-eelampsia(MP)and 23 cases with gevere pre-eclampsia(SP),26 cases of normal pregnant women as control group.Immunohistochemistry and image analytical technique were employed to show the expression of ICAM-1 and VCAM-1.Results The quantities of ICAM-1 and VCAM-1 expressed in the placental vascular endothelial cell were 78.85%(41/52)and 75.00%(39/52)in disease group,26.92%(7/26)and 23.08%(6/26)in control group,there was a significant difference between the two groups(P<0.01).and those in SP patients[100.00%(23/23)and 100.00%(23/23)]were higher than those in MP patients[77.78%(14/18)and 72.22%(13/18)]and GH patients[36.36%(4/11)and 27.27%(3/11)](P<0.05 or<0.01).The quantities of ICAM-1 and VCAM-1 expressed in the placental villous syncytlotrophoblast were cell 38.46%(20/52)and 44.23% (23/52)in disease group,100.00%(26/26)and 96.15%(25/26)in control group,there was a significant difference between the two groups(P<0.01),and those in SP patients[8.70%(2/23)and 13.04%(3/23)]were lower than those in MP patients[44.44%(8/18)and 50.00%(9/18)]and GH patients[90.91%(10/11)and 100.00%(11/11)](P<0.05 or<0.01).Conclusion Abnormal expression of ICAM-1 and VCAM-1 in the placental vascular endothelial and villous syncytiotrophoblast cell in patients with hypertensive disorders in pregnancy may participate in pathogenic process of vascular endothelial damage of hypertensive disorders in pregnancy.

Objective To explore an applicable method for isolation and purification of Cryptosporidium parvum oocysts with high purity, recovery and vigor from mouse feces. Methods Four techniques were used for isolating and purifying C.parvum oocysts from mouse feces: modified saturated saline flotation, percoll gradient centrifugation, CsCl gradient centrifugation and the classical discontinuous sucrose gradient centrifugation. Oocysts received from the methods were used respectively to infect in vitro bovine fallopian tube epithelial cells (BFTE) and the development of the oocysts was examined under microscope after 48 h and 72 h cultivation. Results The number of oocysts received by the classical discontinuous sucrose gradient centrifugation [(2.86?0.08)?107] was significantly higher than that of percoll gradient centrifugation [(1.52?0.08)?107] (P0.05). Oocysts received from CsCl gradient centrifugation showed higher purity than those by discontinuous sucrose gradient centrifugation. Conclusion In comparison to the classical discontinuous sucrose gradient centrifugation, operation of the modified saturated saline flotation is easier and faster, and the purity of oocysts isolated by CsCl gradient centrifugation is higher.

AIM: To study the chemical components and contents of the volatile oil from the Rubia tinctorum L.. METHODS: The volatile oil was extracted by steam distillation-extract method,the chemical constituents of the volatile oil from the herb were identified by gas chromatography-mass spectrometry(GC-MS). RESULTS: Thirty compounds were identified,which accounted for over 86.32% of the volatile oil. CONCLUSION: The major components were theaspirane(17.37%),2-furaldehyde(11.10%),benzeneacetaldehyde(6.01%),eugenol(5.47%),ionone(3.87%),and so on.

Objective To explore the common complications and methods of treatment in the malignant obstruction of upper alimentary tract with stent insertion. Methods With interventional procedure under fluoroscopic guidance fourteen self expanding stents were implanted in twelve patients, including nine with strictures or obstructions of esophagus, three with obstructions of gastroduodenum. Of the fourteen, nine were coated stents and five were uncoated stents. Results All stents were implanted successfully, but complications after the procedure occurred sometimes. There complications included: 1. Food bolus obstructed in three patients. 2. Chest pain occurred in four patients. 3. Tumour overgrowth or hyperplasia of granulation tissue in three patients caused restenosis of gastrointestinal tract. 4. Stent replacement in three patients. 5. Hemorrhage occurred in two patients (over 300 ml) causing threat to life. Conclusions The implantation of self expanding stent is a simple and effective method offering good palliation for upper alimentary tract obstructions. The complications shoud be treated correctly.

Objective To develop an in vitro culture system for Cryptosporidium parvum in Madin-Darby canine kidney(MDCK) cell and observe its life cycle(from desquamate to oocyst).Methods Oocysts of C.parvum were co-cultured with MDCK cells in vitro.Culture condition was optimized and the life cycle of C.parvum investigated.Results The optimal culture conditions for C.parvum in MDCK cells were 2.0?105 cells cultured for 12 h, and infected by 1.0?105 oocysts in the Dulbecco′s Modified Eagle Medium with 5% FBS.Following 72 h co-culture, desquamate, sporozoites, trophozoites, meronts, microgametocytes, macrogametocytes, zygote, thin-wall oocyst, and thick-wall oocyst appeared orderly.Between the 60th and 72th hour, many oocysts emerged.Inoculated by the C.parvum-infected cell culture supernatant at the 48th hour, the immunosuppressed mice became infected.Conclusion The culture system provides a model for propagation of the parasites and demonstrates a complete in vitro life cycle of C.parvum.