Hereditary erythrocyte glucosephosphate isomerase (GPI) deficiency is thefourth most common cause of non-spherocytic hemolytic anemia. A case of GPI deficiencywas confirmed after screening a panel of 18 red cell enzymes and GSH. The GPI variantwas characterized by biochemical parameters including GPI activity in erythrocytes andplasma, low substrate activity, electrophoretic mobility, pH optimum, Michaelis constant(Km), thermostability, and substrate analogue (GAL-6-P) utilization rate. A new GPIvariant was found and was designated as GPI-Guangzhou.

Matrix metalloproteinase (MMP) are a family of zinc-dependent endoproteinases whose enzymatic activity is directed against components of the extracelluar matrix(ECM). Their activities are inhibited by the tissue inhibitor of metalloproteinase (TIMP). MMP exerted an important role in invasion and metastasis of gastrointestinal cancer through degrading the ECM. With further research on inhibition of MMP, synthetic MMP inhibitor will have good prospects in the treatment of tumor invasion and metastasis.

OBJECTIVE The bibliometric index of papers published in Chinese Journal of Nosocomiology in sequential 11 years was analyzed by the citation method in order to get comprehensive understanding of the articles in academic quality and level.METHODS The cited articles in the journal were analyzed and evaluated using quantitative approach on the basis of the data searched from Chinese Medical Citation Index(CMCI) database developed by Medical Library of Chinese PLA.RESULTS The total cites,immediacy index and impact factors of the journal articles were turned to increase in years.Authors were widely distributed,however,mainly concentrated in Beijing,Shanghai,Guangzhou and some developed provinces as well as the affiliated hospitals of the large medical universities.CONCLUSIONS With comparatively high quality in articles which can fully reflect the scientific achievements and development in the field of nosocomial infections in China,the journal is evaluated as one of core journals in medical even in the whole scientific journal collections.

To train the clinical ideation of students majoring in medicine is one of the goals of medical education. Applying the integrated teaching methods to the physiology theory education is helpful to cultivating students′abilities to think and practice the clinical ideation and application,inspiring their interests in study,evoking their ideation and enhancing their abilities to learn initiatively and solving problem independently.

Objectiove To explore the teaching effectiveness of PBL teaching methods the teaching of cardiothoracic surgery.Methods Random clinical medical intern students 2008-2009 in our department were divided into 2 groups,with PBL teaching methods used in experimental group,and traditional teaching methods in the control group.Results The excellent and failure rates of the exam scores of the students in the experimental group were 75% and 4% respectively.while in the control group were 43% and 18% respectively.After statistical treatment,compared with the excellent and failure rates in the experimental group and in the control group,the difference is of high significance.Conclusion PBL teaching method in cardiothoracic surgery in the teaching effectiveness of teaching is better,worthy of promotion.

Objective To explore the effect of follow-up by telephone calls on the medication compliance of patients with cancer pain.Methods Two hundred and forty hospitalized cancer patients suffering from moderate to severe cancer pain were included in the study. All the patients were provided with education on pain treatment before discharge.Then they were randomly assigned to receive telephone follow-up or routine nursing after discharge.The medication compliance were compared 2 months after discharge.Result The medication compliance in the observation group was significantly better than that in the control group.Conclusion Telephone follow-ups can improve medication compliance in patients with cancer pain.

Objective To evaluate the sterilization effect of Er:Yag laser irradiation combined with 2%chlorhexidine gel on single tube premolar enterococcus faecalis infection model.Methods After disinfection test was performed without molars bacterial infection,200 single rooted premolar root canal were selected.10μL enterococcus faecalis( ATCC 29212) bacterium suspension were inoculated into single intratracheal with high pressure sterilization and and then were cultured for 2 weeks.200 infected premolar model were divided into four groups:control group (n=50),A group (n=50),B group (n=50) and C group (n=50).The control group did not receive any disinfec-tion treatment,group A received Er:Yag laser irradiation and 2%chlorhexidine gel disinfection,B group received only Er:Yag laser irradiation,the C group received only 2% chlorhexidine gel disinfection.After disinfection,premolars were put into 10mL sterile water,mixed thoroughly,and then 1mL liquid were taken and diluted by10 times.Then the 200μL dilutions lipuid were coated on BHI agar plate,and plate could be selected for counting colony count.Results The average number of colonies was(127 ±12) in the control group,average number of group A(10 ±3) and which was lower than that group C (48 ±6)(t=12.005,P=0.000) and group C (43 ±5) (t=19.277,P=0.000). There were only 2 cases,which had obvious ablation phenomena in group A.Conclusion Disinfection effect of Er:Yag laser irradiation combined with 2%chlorhexidine gel is better than that of Er:Yag laser irradiation or 2% chlo-rhexidine gel disinfection.The incidence rate of the ablation phenomenon was low.

Allogeneic peripheral blood stem cells transplantation is the most effective method for maligant hematological disease. The author reviewed the indication of acceptance of HLA mismatch kinds and the prediction of the results by selecting the measures such as pretreatment,mobilization,stem cells disposal,immunity reconstruction,graft failure management in order to improve the engraft rate.

AIM: To explore the effects and mechanisms of classic prescription Jiajian Yun? Decoction(JJYND) on ventricular remodeling. METHODS: Two kinds of ventricular remodeling model were induced by injecting L-thyroxine intraperitoneally or banding abdominal aorta in rats.The effects of JJYND on heart index(HI) and concentrations of neuroendocrine factors such as serum nitric oxide(NO),myocardium angiotensin Ⅱ(Ang Ⅱ),endothelin-1(ET-1),activities of glutathione peroxidase(GSH-Px),superoxide dismutase(SOD),concentration of myocardium hydroxyproline(Hyp),cross section area of cardiomyocyte were determined. RESULTS: JJYND could significantly reduce the heart index,cardiomyocyte cross section area,elevate serum NO concentration,restrain myocardium Ang Ⅱ,ET-1,Hyp concentrations and improve myocardium GSH-Px、SOD activities. CONCLUSION: Prescription JJYND has good effects on attenuating ventricular remodeling.

Aging is a complex pathological and physiological phenomenon characterized by the decline of many functions in human. Several lines of experimentation showed that the defect of the mitochondria function is one of the driving forces for the progress of aging. During the development of aging, the alteration of biology of mitochondria, the accumulation of the generation of "free radical"in mitochondria, the mutation in mitochondria genomes and the control of the progress of death by mitochondria were suggested to be a number of novel mechanisms for mitochondria in aging. The experiment data focused on the role of mitochondria in aging are reviewed.