BACKGROUND: Deposition of lipofuscin (LF), decrease of superoxide dismutase (SOD) level and increase of malondialdehyde (MDA) level are important manifestations of senility. Some Chinese medicinal herbs have obvious advantage in delaying senility.OBJECTIVE: To explore the effects of fuling shengmai yin (FLSMY)serum on SOD, MDA and LF level of rat hippocampal neurons in vitro with vitamin E serum and blank serum as controls.DESIGN: Randomized controlled study.SETTING: Experimental Animal Department of Tongji Medical College,Huazhong Science and Technology University; Cell Culture Center of Anatomy Teaching and Research Section of Tongji Medical College,Huazhong Science and Technology University; Scientific Research Department of Wuhan Municipal Hospital of Traditional Chinese Medicine.MATERIALS: From October 2000 to February 2001, the experiment was conducted at the Experimental Animal Department of Tongji Medical College as well as Cell Culture Center of Anatomy Teaching and Research Section of Tongji Medical College, Huazhong Science and Technology University. Six Japanese male white rabbits with big ears, fed normally and weighting (2.5±0.1)kg, were divided into three groups with 2 in each group.Thirty newly born SD rats aged 1-2 days, of clean grade and either gender,were selected.METHODS: [1] Hippocampal neurons were inoculated to 24-well culture plates with the density of 2.5×105/L. Cells in each well were randomized into three groups: A. FLSMY group (fuling shengmaiyin composed of Poria,Radix Ginseng, Radix Ophiopogonis and Fructus Schisandrae; each milliliter contained 1.5 gram of the herbs); B. drug control group (serum containing vitamin E); C. blank control group (blank serum). Each group had 8 wells. After 24 hours, 1 mL serum containing FLSMY, 1ml serum containing vitamin E, or 1 mL blank serum was added into each plate well of corresponding groups. [2] On the 6th day of cell culture, XO-HPX reaction system was added to induce cell injury, in which senility model was established by producing hyperoxide anion free radicals. [3] In the process of cell culture different drug was added to the corresponding different group plate well during replacement of culture fluid each time. [4] The level of SOD, MDA, and LF was detected after 15 days of culture.MAIN OUTCOME MEASURES: Level of SOD, MDA and LF in rat hippocampal neurons.RESULTS: SOD level was higher in FLSMY group than in blank control group (P ＜ 0.01), whereas MDA level in FLSMY group was lower than that in vitamin E group (P ＜ 0.05); LF level was lower in FLSMY group than in blank group (P ＞ 0.05).CONCLUSION: FLSMY as a drink is composed of traditional Chinese medicinal herbs: Poria, Radix Ginseng, Radix Ophiopogonis and Fructus Schisandrae; each has the properties of anti-oxidation and eliminating free radicals. FLSMY can strengthen SOD activity, lower MDA level, and inhibit LF formation. Therefore, FLSMY can delay senility through protecting neurons.

OBJECTIVE: Superoxide dismutase is one of the families of enzymes which is ubiquitous, and efficiently catalyzes the dismutation of superoxide anions.There are three superoxide dismutases in human brain that are of benefit to ischemic damage. In recent years, transgenic and knockout mutant mice has been widely used in the researches of superoxide dismutases for its protective effect in cerebral ischemia.DATA SOURCES: Using the terms "Chan PH and superoxide dismutase and cerebral ischemia", the relative articles published in the English from January 1991 to December 2003, were identified by searching the MEDLINE database with computer.STUDY SELECTION: The trials with transgenic and knockout mutant mice were identified preliminarily, then all remainders were searched in full content and were divided into intervention group and controlled group, the random cases were accepted, non-random excluded.DATA EXTRACTION: A total of 38 fully studies related to cerebral ischemia and superoxide dismutase were collected, among them, 13 random cases were accepted, and 25 excluded which included 15 repeated studies and 10 irrelevant studies.DATA SYNTHESIS: Reactive oxygen species involved in redox signaling pathways in ischemic cerebral, it can also cause oxidative damage of some macromolecules cell such as lipids, proteins and neucleic acid. In the ischemic brain tissue, overproduced oxygen radicals and consumption of superoxide dismutases lead to tissue damage. Copper-zinc superoxide dismutase, manganese superoxide dismutase and extracellular superoxide dismutase have the same function which can decompose superoxide anion radicals to form H2O2 that are scavenged further, thereby ameliorate edema formation,infarction and cell death, improve the function of ischemic cerebral tissue further.CONCLUSION: Increased oxygen free radicals is an important mechanism in ischemic cerebral damage, all three SOD can ameliorate cerebral ischemia. The protect effect of three superoxide dismutase is related to DNA repairing enzyme, transcription factor, apoptosis proteins and signal transduction pathway.

Women diagnosed with breast cancer have a significantly higher risk of developing contralateral breast carcinoma.When the dignosis ofbilateral breast cancer (BBC)is established,the challenges for the doctors are to determine contralateral breast carcinoma is metastasis or primary lesions.The pathological type,receptor status,biological behavior and the prognosis of bilateral breast cancer are the issues have to be considered.On the other hand,we discuss which type of breast cancer trend to be bilateral breast cancer,thus more effective follow-up and preventive procedures would be applied on this part of patients to improve their quality of life and the curative rate.

Matrix metalloproteinase (MMP) are a family of zinc-dependent endoproteinases whose enzymatic activity is directed against components of the extracelluar matrix(ECM). Their activities are inhibited by the tissue inhibitor of metalloproteinase (TIMP). MMP exerted an important role in invasion and metastasis of gastrointestinal cancer through degrading the ECM. With further research on inhibition of MMP, synthetic MMP inhibitor will have good prospects in the treatment of tumor invasion and metastasis.

Objective Through the statistical analysis on literature on asthenia cold syndrome in recent ten years,to comprehend the present situation of studies on the feature and objectivization of symptom distribution of asthenia cold syndrome.Methods Collect literature on asthenia cold syndrome from the periodicals of traditional Chinese medicine and integrated traditional and western medicine from the year 1996 to 2006.The described features and objective indices of symptom distribution of this syndrome were systematically analyzed and summarized.Results In the literature,the diagnostic criteria of asthenia cold syndrome were made mainly by the authors themselves,few referring to others',being different in the descriptions of symptom distribution.The researches on objectivization indices were scattered,most of which took the single or multiple index(es) as subject(s).Conclusion Cold limbs,tiredness,aversion to cold,and loose stools are possibly significant in the diagnosis of asthenia cold syndrome.The way of the studies on diagnostic criteria and its objectivization of asthenia cold syndrome need to be improved.

OBJECTIVE: To study the bioequivalence of domestic oxcarbazepine dispersible tablets. METHODS: 20 healthy male volunteers were randomly given oxcarbazepine dispersible tablets (test tablet) and oxcarbazepine tablets (reference tablet) via p.o. The plasma concentration of active metabolite (MHD) of oxcarbazepine were determined by HPLC. 3p97 software was used to calculate pharmacokinetic parameters and the bioequivalence of oxcarbazepine were evaluated. RESULTS: After treatment main pharmacokinetic parameters of test tablet vs. reference tablet were as follows: Cmax(4.231?0.841) ?g?mL-1 vs. (4.350?0.861) ?g?mL-1; tmax(4.13?0.43) h vs. (4.28?0.44) h; t1/2(14.17?2.66) h vs. (14.44?2.09) h; AUC0～60 (113.00?22.25) mg?h?L-1 vs. (118.11?14.20) mg?h?L-1; AUC0～∞(126.35?20.94) mg?h?L-1 vs. (130.54?16.17) mg?h?L-1. The relative bioavailability of test tablet to reference tablet was (95.67?12.80)%. CONCLUSION: Two kinds of tablets were bioequivalent.

Hereditary erythrocyte glucosephosphate isomerase (GPI) deficiency is thefourth most common cause of non-spherocytic hemolytic anemia. A case of GPI deficiencywas confirmed after screening a panel of 18 red cell enzymes and GSH. The GPI variantwas characterized by biochemical parameters including GPI activity in erythrocytes andplasma, low substrate activity, electrophoretic mobility, pH optimum, Michaelis constant(Km), thermostability, and substrate analogue (GAL-6-P) utilization rate. A new GPIvariant was found and was designated as GPI-Guangzhou.

OBJECTIVE The bibliometric index of papers published in Chinese Journal of Nosocomiology in sequential 11 years was analyzed by the citation method in order to get comprehensive understanding of the articles in academic quality and level.METHODS The cited articles in the journal were analyzed and evaluated using quantitative approach on the basis of the data searched from Chinese Medical Citation Index(CMCI) database developed by Medical Library of Chinese PLA.RESULTS The total cites,immediacy index and impact factors of the journal articles were turned to increase in years.Authors were widely distributed,however,mainly concentrated in Beijing,Shanghai,Guangzhou and some developed provinces as well as the affiliated hospitals of the large medical universities.CONCLUSIONS With comparatively high quality in articles which can fully reflect the scientific achievements and development in the field of nosocomial infections in China,the journal is evaluated as one of core journals in medical even in the whole scientific journal collections.

AIM: To explore the effects and mechanisms of classic prescription Jiajian Yun? Decoction(JJYND) on ventricular remodeling. METHODS: Two kinds of ventricular remodeling model were induced by injecting L-thyroxine intraperitoneally or banding abdominal aorta in rats.The effects of JJYND on heart index(HI) and concentrations of neuroendocrine factors such as serum nitric oxide(NO),myocardium angiotensin Ⅱ(Ang Ⅱ),endothelin-1(ET-1),activities of glutathione peroxidase(GSH-Px),superoxide dismutase(SOD),concentration of myocardium hydroxyproline(Hyp),cross section area of cardiomyocyte were determined. RESULTS: JJYND could significantly reduce the heart index,cardiomyocyte cross section area,elevate serum NO concentration,restrain myocardium Ang Ⅱ,ET-1,Hyp concentrations and improve myocardium GSH-Px、SOD activities. CONCLUSION: Prescription JJYND has good effects on attenuating ventricular remodeling.

Aging is a complex pathological and physiological phenomenon characterized by the decline of many functions in human. Several lines of experimentation showed that the defect of the mitochondria function is one of the driving forces for the progress of aging. During the development of aging, the alteration of biology of mitochondria, the accumulation of the generation of "free radical"in mitochondria, the mutation in mitochondria genomes and the control of the progress of death by mitochondria were suggested to be a number of novel mechanisms for mitochondria in aging. The experiment data focused on the role of mitochondria in aging are reviewed.